HPLC–ESI-Q-TOF-MS for a comprehensive characterization of bioactive phenolic compounds in cucumber whole fruit extract

Husk and pellicle as the agri-food waste in the walnut-product industry are in soaring demand because of their rich polyphenol content. This study investigated the differential compounds related to walnut polyphenol between husk and pellicle during fruit development stage. By using ultra-high performance liquid chromatography-quadrupole-orbitrap (UHPLC-Q-Orbitrap), a total of 110 bioactive components, including hydrolysable tannins, flavonoids, phenolic acids and quinones, were tentatively identified, 33 of which were different between husk and pellicle. The trend of dynamic content of 16 polyphenols was clarified during walnut development stage by high-performance liquid chromatography (HPLC). This is the first time to comprehensive identification of phenolic compounds in walnut husk and pellicle, and our results indicated that the pellicle is a rich resource of polyphenols. The dynamic trend of some polyphenols was consistent with total phenols. The comprehensive characterization of walnut polyphenol and quantification of main phenolic compounds will be beneficial for understanding the potential application value of walnut and for exploiting its metabolism pathway.

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Identification and characterization of phenolic compounds in hydro methanolic extract of Achyranthes aspera (HMEA) by UPLC and MALDI-TOF-MS and in vivo antioxidant activity

Oriental Pharmacy and Experimental Medicine ◽

10.1007/s13596-012-0085-z ◽

2012 ◽

Vol 13 (1) ◽

pp. 51-59 ◽

Cited By ~ 9

Author(s):

Chandradeo Narayan ◽

Arvind Kumar

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Methanolic Extract ◽

Maldi Tof Ms ◽

Achyranthes Aspera ◽

Maldi Tof ◽

Identification And Characterization ◽

Tof Ms

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Comprehensive Characterization of Extractable Phenolic Compounds by UPLC-PDA-ESI-QqQ of Buddleja scordioides Plants Elicited with Salicylic Acid

Journal of Chemistry ◽

10.1155/2018/4536970 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

J. O. Díaz-Rivas ◽

R. F. González-Laredo ◽

J. A. Chávez-Simental ◽

J. B. Montoya-Ayón ◽

M. R. Moreno-Jiménez ◽

...

Keyword(s):

Biological Activity ◽

Salicylic Acid ◽

Phenolic Compounds ◽

Phenolic Profile ◽

Phytochemical Composition ◽

Biological Potential ◽

Medicinal Properties ◽

Metabolites Production ◽

Comprehensive Characterization

Buddleja scordioides has a rich phytochemical composition and reported medicinal properties. An increase in its secondary metabolites production could improve its functional properties. A strategy to enhance its biological potential is to subject the plant to elicitation with phytohormones such as salicylic acid under controlled environmental conditions. The present study explores the effect of exogenous application of three salicylic acid levels as elicitation treatment in B. scordioides plants. Phenolic profile, enzymatic activities, and antioxidant capacity were evaluated. Elicitation with 100 µM of salicylic acid resulted in the biosynthesis of phenolic compounds with recognized biological activity.

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