Enhanced innate immune parameters in Labeo rohita (Ham.) following oral administration of Bacillus subtilis

2008 ◽  
Vol 24 (2) ◽  
pp. 168-172 ◽  
Author(s):  
Rajesh Kumar ◽  
S.C. Mukherjee ◽  
Ritesh Ranjan ◽  
S.K. Nayak
Keyword(s):  
Bacillus Subtilis ◽  
Oral Administration ◽  
Labeo Rohita ◽  
Innate Immune ◽  
Immune Parameters

Effects of dietary inulin and heat-inactivated Bacillus subtilis on gilthead seabream (Sparus aurata L.) innate immune parameters

2012 ◽  
Vol 3 (1) ◽  
pp. 77-81 ◽  
Author(s):  
R. Cerezuela ◽  
A. Cuesta ◽  
J. Meseguer ◽  
M. Esteban
Keyword(s):  
Bacillus Subtilis ◽  
Sparus Aurata ◽  
Innate Immune ◽  
Gilthead Seabream ◽  
Cytotoxic Activities ◽  
Feeding Trial ◽  
Complement Activity ◽  
Immune Parameters ◽  
Group B ◽  
Dietary Treatments

In the present study, a feeding trial was conducted to evaluate the effect of inulin and heat-inactivated Bacillus subtilis, single or combined, on several innate immune activities of gilthead seabream (Sparus aurata). Forty-eight specimens were randomly assigned to four dietary treatments: 0 (control), inulin (10 g/kg, prebiotic group), B. subtilis (107 cfu/g, probiotic group), or B. subtilis + inulin (107 cfu/g + 10 g/kg, synbiotic group). After two and four weeks, six fish of each group were sampled, with the main innate immune parameters (natural haemolytic complement activity, serum and leucocyte peroxidase, phagocytosis, respiratory burst, and cytotoxic activities) being determined. Inulin or heat-inactivated B. subtilis failed to significantly stimulate the innate immune parameters assayed, although some activities showed no significant increase through these treatments. A combination of inulin and B. subtilis resulted in an increase of such parameters, with the haemolytic complement activity being the only one significantly stimulated. To conclude, inulin and B. subtilis, when administered as a synbiotic, have a synergistic effect and enhance some innate immune parameters of gilthead seabream.

Effect of dietary supplemented andrographolide on growth, non-specific immune parameters and resistance against Aeromonas hydrophila in Labeo rohita (Hamilton)

2013 ◽  
Vol 35 (5) ◽  
pp. 1433-1441 ◽  
Author(s):  
Kusunur Ahamed Basha ◽  
Ram Prakash Raman ◽  
Kurcheti Pani Prasad ◽  
Kundan Kumar ◽  
Ezhil Nilavan ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Labeo Rohita ◽  
Immune Parameters

scholarly journals Estimation of permeability to bacteriophages of intestinal mucosa of chickens with eimeriosis

2019 ◽  
Vol 49 (2) ◽  
pp. 57-63
Author(s):  
N. V. Davydova ◽  
V. Yu. Koptev ◽  
Yu. N. Kozlova ◽  
L. I. Sulimova ◽  
V. N. Afonyushkin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oral Administration ◽  
Protective Effect ◽  
Intestinal Mucosa ◽  
Clinical Picture ◽  
Biological Material ◽  
Probiotic Strain ◽  
Field Isolates ◽  
Different Types ◽  
Inflammatory Processes

In the course of the study permeability of intestinal mucosa of chickens suffering from eimeriosis while treating them with various veterinary drugs, including probiotics, was evaluated. The simulation of a typical clinical picture of eimeriosis was carried out by oral administration of suspension with coccidial oocysts (1.6 × 105/head) using a probe. To create different forms and different intensity of inflammatory processes, chickens that received various anticoccidial preparations and probiotic strain Bacillus subtilis were infected with eimeria. According to the data from an autopsy, it was found that the use of a spore probiotic based on Bacillus subtilis and anticoccidial drugs containing robenidine hydrochloride and salinomycin had a positive protective effect when treating chickens from eimeriosis. A similar picture was observed when assessing permeability of intestinal mucosa as affected by bacteriophage, whereby permeability decreased with the use of probiotics and the above-mentioned active agents. In general, the decrease in productivity was significant in all groups. However, the effect of spore-based probiotics was quite pronounced against the background of eimeria polyresistance. In the situation where anticoccidial drugs are less effective, the use of a spore-based probiotic can have a noticeable protective effect. The effect of all anticoccidial drugs under study on the concentration of oocysts and the state of the mucosa was insignificant, which indicated polyresistance of different types of eimeria isolated from biological material to these drugs. The analysis of the intestinal mucosa integrity, based on the study of mucosa permeability to bacteriophages and a Johnson and Reid scoring procedure showed that a spore probiotic based on B. subtilis and anticoccidial drugs containing robenidine and salinomycin had the best protective effect against eimeriosis caused by field isolates of eimeria. When treating chickens suffering from eimeriosis caused by polyresistant forms of E. acervulina and E. tenella, it is advisable to use probiotics alongside with drugs based on robenidine and salinomycin.

scholarly journals Orally administered β-glucan improves the hemolytic activity of the complement system in horses

2021 ◽  
Vol 14 (4) ◽  
pp. 835-840
Author(s):  
Taline Scalco Picetti ◽  
Lucas de Figueiredo Soveral ◽  
Rovian Miotto ◽  
Luana Marina Scheer Erpen ◽  
Yasmin Kreutz ◽  
...  
Keyword(s):  
Hemolytic Activity ◽  
Innate Immune ◽  
Stressful Events ◽  
The Other ◽  
Control Group ◽  
Classical Pathway ◽  
Immune Parameters ◽  
Thoroughbred Horses ◽  
Hematological Analysis ◽  
The Complement System

Background and Aim: Immune-modulating molecules mainly act on innate immune cells, which are central to early defense against invading pathogens and contribute to developing adaptive immunity. Yeast-extracted β-glucan, a model immune-modulating molecule, is widely used in several animal species; however, its effect on horse immune parameters has not been thoroughly investigated yet. This study aimed to evaluate the effects of orally administered β-glucan on selected innate immune parameters in horses. Materials and Methods: Eighteen thoroughbred horses were assigned equally into three groups as follows: One control group (no β-glucan) and two β-glucan experimental groups (one received 125 mg and the other 2 g of β-glucan per day for 28 days). Blood samples were collected before and at the end of the experiment for hematological analysis, whole blood phagocytosis, respiratory burst assays, and to assess the serum lysozyme and complement hemolytic activities. Results: At the end of the experiment, significant decreases (p<0.05) in monocyte numbers were observed in the control horses (258.8±45.9 vs. 115.3±41.5) and in those fed 125 mg/day of β-glucan (208.8±72.3 vs. 99.2±60.7), whereas a significant increase in numbers was noted in the horses that were fed 2 g/day of β-glucan (303.5±45.8 vs. 429.8±86.0; p<0.05). The natural hemolytic activity of the complement was higher only in horses fed 2 g/day of β-glucan (p=0.018) compared to the other groups. The hemolytic activity in the classical pathway was higher in those fed 125 mg/day (p=0.0035) and 2 g/day of β-glucan (p=0.0001). Conclusion: β-glucan improves important innate immune parameters and might be fed to horses before stressful events.

Sign in / Sign up

Export Citation Format

Share Document