Orally administered β-glucan improves the hemolytic activity of the complement system in horses

Background and Aim: Immune-modulating molecules mainly act on innate immune cells, which are central to early defense against invading pathogens and contribute to developing adaptive immunity. Yeast-extracted β-glucan, a model immune-modulating molecule, is widely used in several animal species; however, its effect on horse immune parameters has not been thoroughly investigated yet. This study aimed to evaluate the effects of orally administered β-glucan on selected innate immune parameters in horses. Materials and Methods: Eighteen thoroughbred horses were assigned equally into three groups as follows: One control group (no β-glucan) and two β-glucan experimental groups (one received 125 mg and the other 2 g of β-glucan per day for 28 days). Blood samples were collected before and at the end of the experiment for hematological analysis, whole blood phagocytosis, respiratory burst assays, and to assess the serum lysozyme and complement hemolytic activities. Results: At the end of the experiment, significant decreases (p<0.05) in monocyte numbers were observed in the control horses (258.8±45.9 vs. 115.3±41.5) and in those fed 125 mg/day of β-glucan (208.8±72.3 vs. 99.2±60.7), whereas a significant increase in numbers was noted in the horses that were fed 2 g/day of β-glucan (303.5±45.8 vs. 429.8±86.0; p<0.05). The natural hemolytic activity of the complement was higher only in horses fed 2 g/day of β-glucan (p=0.018) compared to the other groups. The hemolytic activity in the classical pathway was higher in those fed 125 mg/day (p=0.0035) and 2 g/day of β-glucan (p=0.0001). Conclusion: β-glucan improves important innate immune parameters and might be fed to horses before stressful events.

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Das Komplementsystem

Hämostaseologie ◽

10.5482/ha-1191 ◽

2012 ◽

Vol 32 (04) ◽

pp. 276-285 ◽

Cited By ~ 3

Author(s):

V. Frauenknecht ◽

V. Schroeder

Keyword(s):

Coronary Artery Disease ◽

Complement System ◽

Innate Immune System ◽

Innate Immune ◽

The Other ◽

Atherosclerotic Plaques ◽

Inflammatory Processes ◽

Artery Disease ◽

The Complement System ◽

Novel Therapeutic

SummaryAtherosclerotic diseases such as coronary artery disease and ischaemic stroke are caused by chronic inflammation in arterial vessel walls. The complement system is part of the innate immune system. It is involved in many processes contributing to onset and development of atherosclerotic plaques up to the final stage of acute thrombotic events. This is due to its prominent role in inflammatory processes. In addition, there is increasing evidence that interactions between complement and coagulation provide a link between inflammation and thrombosis. On the other hand, the complement system also has an atheroprotective function through the clearance of apoptotic material.The knowledge of these complex mechanisms will become increasingly important, also for clinicians, since it may lead to novel therapeutic and diagnostic options. Therapies targeting the complement system have the potential to reduce tissue damage caused by acute ischaemic events. Whether early anti-inflammatory and anti-complement therapy may be able to prevent atherosclerosis, remains a hot topic for research.

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Skin Mucosal, Serum Immunity and Antioxidant Capacity of Common Carp (Cyprinus carpio) Fed Artemisia (Artemisia annua)

Annals of Animal Science ◽

10.2478/aoas-2020-0011 ◽

2020 ◽

Vol 20 (3) ◽

pp. 1011-1027 ◽

Cited By ~ 13

Author(s):

Iman Sarhadi ◽

Ebrahim Alizadeh ◽

Ehsan Ahmadifar ◽

Hossein Adineh ◽

Mahmoud A.O. Dawood

Keyword(s):

Antioxidant Capacity ◽

Growth Performance ◽

Common Carp ◽

Cyprinus Carpio ◽

Artemisia Annua ◽

The Other ◽

Control Group ◽

Feed Conversion ◽

Skin Mucus ◽

Immune Parameters

AbstractIn this study, the effect of artemisia (Artemisia annua) leaves extract (ALE) on the immune system, growth performance and antioxidant capacity of common carp (Cyprinus carpio) was investigated. Fish with initial weight (90.32 ± 1.12 g) were fed various levels of ALE (0, 0.5, 1 and 1.5 g/kg) for 8 weeks. The results showed that the growth performance (FW, WG, SGR, PER, and LER) in fish fed 1 g/kg ALE was increased significantly (P<0.05) and the feed conversion ratio (FCR) was improved in fish fed 1 and 1.5 g/kg ALE in comparison to the control group (P<0.05). Skin mucus total protein was higher in fish fed diet containing 0.5 g/kg ALE than the other experimental groups (P<0.05), while the lysozyme, immunoglobulin and protease was increased by increasing of ALE in diets. Respiratory burst activity and serum immune parameters were higher in fish fed 0.5 g/kg ALE than the other treatments (P<0.05). Increasing of catalase (CAT) and decreasing of malondialdehyde (MDA) were observed in fish fed ALE, while glutathione peroxidase (GPx) increased only in fish fed 0.5 g/kg ALE. Hence, it can be concluded that ALE has considerable potential as a natural immunostimulant and growth promotor supplement for the common carp’s aquaculture.

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Structure and activation of C1, the complex initiating the classical pathway of the complement cascade

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1616998114 ◽

2017 ◽

Vol 114 (5) ◽

pp. 986-991 ◽

Cited By ~ 34

Author(s):

Simon A. Mortensen ◽

Bjoern Sander ◽

Rasmus K. Jensen ◽

Jan Skov Pedersen ◽

Monika M. Golas ◽

...

Keyword(s):

Serine Protease ◽

Innate Immune System ◽

Innate Immune ◽

Lectin Pathway ◽

Classical Pathway ◽

Structural Studies ◽

Proteolytic Activation ◽

Molecular Patterns ◽

The Complement System ◽

Recognition Molecule

The complement system is an important antimicrobial and inflammation-generating component of the innate immune system. The classical pathway of complement is activated upon binding of the 774-kDa C1 complex, consisting of the recognition molecule C1q and the tetrameric protease complex C1r2s2, to a variety of activators presenting specific molecular patterns such as IgG- and IgM-containing immune complexes. A canonical model entails a C1r2s2with its serine protease domains tightly packed together in the center of C1 and an intricate intramolecular reaction mechanism for activation of C1r and C1s, induced upon C1 binding to the activator. Here, we show that the serine protease domains of C1r and C1s are located at the periphery of the C1r2s2tetramer both when alone or within the nonactivated C1 complex. Our structural studies indicate that the C1 complex adopts a conformation incompatible with intramolecular activation of C1, suggesting instead that intermolecular proteolytic activation between neighboring C1 complexes bound to a complement activating surface occurs. Our results rationalize how a multitude of structurally unrelated molecular patterns can activate C1 and suggests a conserved mechanism for complement activation through the classical and the related lectin pathway.

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Combined Effects of Leucas aspera, Oxy-Cyclodextrin and Bentonite on the Growth, Serum Biochemistry, and the Expression of Immune-Related Gene in Nile Tilapia (Oreochromis niloticus)

Turkish Journal of Fisheries and Aquatic Sciences ◽

10.4194/1303-2712-v21_3_05 ◽

2020 ◽

Vol 21 (03) ◽

pp. 147-158

Author(s):

Amitha Kurian ◽

Sreeja Lakshmi ◽

Femi John Fawole ◽

Caterina Faggio ◽

Preetham Elumalai

Keyword(s):

Nile Tilapia ◽

Digestive Enzyme ◽

Juvenile Fish ◽

Serum Biochemistry ◽

Innate Immune ◽

Control Group ◽

Leucas Aspera ◽

Immune Parameters ◽

Digestive Enzyme Activities ◽

Immune Related Genes

This study investigated the effects of a combination of Leucas aspera, Oxy-cyclodextrin and sodium bentonite (LOB) on growth, digestive enzyme activity, innate immune response, haematology, and expression of immune-related genes in Nile tilapia. A total of 240 juvenile fish (20.15±0.05g) were randomly distributed into four dietary groups in triplicate and fed respective diets containing a graded level of LOB at 0 g kg-1 (Control), 0.3 g kg-1 (T1), 0.6 g kg-1 (T2) and 0.9 g kg-1 diet (T3) for 60 days. After 60 days, higher growth was observed in fish fed T2 diet (P < 0.05). Digestive enzyme activities and innate immune parameters were significantly higher in T3 group. Some of the haematological parameters reported statistically higher counts in T2 group (P<0.05), whereas erythrocyte indices and WBC counts were significantly higher in T3 group. Liver- kidney activities were recorded low in T3 group. Urea and creatinine were higher in control group, whereas T2 group recorded the least value. The highest relative expression of IL-1β, IgM-heavy chain, TGF-β and IFN-γ were recorded in T2 group, but TNF-α was upregulated in T3 group. The results showed that 0.6 - 0.9 g kg1 of LOB is recommended for inclusion in diet.

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Detection and Assessment of Interleukin 6 in Irreversible Pulp Inflamation

Revista de Chimie ◽

10.37358/rc.17.2.5445 ◽

2017 ◽

Vol 68 (2) ◽

pp. 323-327

Author(s):

Cristian Levente Giuroiu ◽

Maria Vataman ◽

Gabriel Melian ◽

Dragos Bularda ◽

Ludmila Lozneanu ◽

...

Keyword(s):

Dental Caries ◽

Interleukin 6 ◽

Dental Pulp ◽

The Other ◽

Control Group ◽

Study Group ◽

Pulp Tissue ◽

Immunohistochemical Technique ◽

Systemic Distribution ◽

Cell Zone

The study aimed to assess the number, localization and distribution of interleukin 6 (IL-6) positive cells in healthy pulp, acute and chronic pulpitis. The study group included 48 patients aged between 18-72, treated in University of Medicine and Pharmacy Grigore T. Popa Iasi, Romania. The pulpectomy was performed on 42 patients diagnosed with acute and chronic pulpitis. The other 6 patients, without signs of dental caries or periodontal disease, were submitted to extractions of teeth for orthodontic purposes, with pulpectomy performed before extraction. The pulp samples were examined with optic microscope. The detection and assessment of IL-6 were performed using immunohistochemical technique. Data were statistically analysed using non-parametric tests. According to morphopathological criteria, 42.85% were classified as acute pulpitis and 57.14% as chronic pulpitis. The pulp samples in control group were not associated with IL-6 positive cells. The analysis of all samples with acute and chronic pulpitis identified 73.80% samples with IL-6 and 26.20% associated with the absence of IL-6. The highest frequency of IL-6 positive cells was recorded in rich-cell zone of crown dental pulp. The systemic distribution of IL-6 positive cells was mostly diffused without well-defined orientation. IL-6 release in acute and chronic pulpitis is significantly higher comparing with healthy pulp tissue.

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Antimicrobial Effect of 940 nm Diode Laser based on Photolysis of Hydrogen Peroxide in the Treatment of Periodontal Disease

Revista de Chimie ◽

10.37358/rc.18.8.6478 ◽

2018 ◽

Vol 69 (8) ◽

pp. 2081-2088 ◽

Cited By ~ 1

Author(s):

Alin Alexandru Odor ◽

Edwin Sever Bechir ◽

Deborah Violant ◽

Victoria Badea

Keyword(s):

Hydrogen Peroxide ◽

Laser Therapy ◽

Diode Laser ◽

Antimicrobial Effect ◽

The Other ◽

Control Group ◽

Group 4 ◽

Periodontal Bacteria ◽

Severe Periodontitis ◽

Before And After

Moderate and severe periodontitis represents a challenge in the non-surgical periodontal therapy. Due to the lack of evidence regarding the antimicrobial effectiveness of 940 nm diode laser in periodontal treatment, this study aimed to evaluate the antimicrobial effect of hydrogen peroxide (H2O2) photolysis performed with 940 nm diode laser in the treatment of moderate and severe periodontitis. Twenty-five patients with 100 teeth were selected for this pilot study. The test teeth were randomly assigned to one of the four treatment groups: Group 1: scaling and root planning (SRP) (control group); and the following experimental groups: Group 2: H2O2; Group 3: 940 nm diode laser therapy; Group 4: 940 nm diode laser therapy and H2O2. Clinical examinations, like probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were performed before and after the treatment. The microbiological evaluation, effectuated before and after the treatment, included nine periodontal bacteria species and investigated by means of real-time PCR assay. The clinical and bacterial differences in the tested groups, was assessed between control group and the other three experimental groups, as well as between the experimental groups. The total bacteria load was reduced for all four studied groups. Group 4 (diode laser + H2O2) showed significant bacterial reduction of the major periodontal bacteria like Pg., Tf., Td., Pi., Pm., Fn (p[0.001) than the other 3 groups (p]0.001). Also the periodontal clinical parameters, like PD, CAL and BOP showed a significant reduction after the photolysis of H2O2 with the 940 nm diode laser (p[0.001). Differences between tested groups showed a significant beneficial results in regard to Group 4.It is suggested that the photoactivation of H2O2 with the 940 nm diode laser can be used successfully in adjunctive to the non-surgical periodontal treatment as a bactericidal tool.

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Apoptotic Effects of Melittin on 4T1 Breast Cancer Cell Line is associated with Up Regulation of Mfn1 and Drp1 mRNA Expression

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666200211091451 ◽

2020 ◽

Vol 20 (7) ◽

pp. 790-799 ◽

Cited By ~ 2

Author(s):

Farnaz D. Moghaddam ◽

Pejman Mortazavi ◽

Somayeh Hamedi ◽

Mohammad Nabiuni ◽

Nasim H. Roodbari

Keyword(s):

Breast Cancer ◽

Mrna Expression ◽

Hemolytic Activity ◽

Breast Cancer Cells ◽

Flow Cytometric Analysis ◽

Control Group ◽

Flow Cytometric ◽

4T1 Cells ◽

4T1 Breast Cancer ◽

Apoptotic Effects

Background and Purpose: Melittin, as the main ingredient of honeybee venom, that has shown anticancer properties. The present study aimed at investigating the cytotoxic impacts of melittin on 4T1 breast cancer cells. Methods: Hemolytic activity of different concentrations (0.125, 0.25, 0.5, 1, 2, 4, 8μg/ml) of melittin was assayed and then cytotoxicity of selected concentrations of melittin (2, 4, 8, 16, 32, and 64μg/ml), 2 and 4μg/ml of cisplatin and 0.513, 0.295 and 0.123μg/ml of doxorubicin was evaluated on 4T1 cells using MTT assay. We used Morphological evaluation and flow cytometric analysis was used. Real time PCR was also used to determine mRNA expression of Mfn1 and Drp1 genes. Results: All compounds showed anti-proliferative effects on the tumor cell line with different potencies. Melittin had higher cytotoxicity against 4T1 breast cancer cells (IC50= 32μg/ml-72h) and higher hemolytic activity (HD50= 1μg/ml), as compared to cisplatin and doxorubicin. Mellitin at 16 and 32μg/ml showed apoptotic effects on 4T1 cells according to the flow cytometric analysis. The Real time PCR analysis of Drp1 and Mfn1 expression in cells treated with 16μg/ml of melittin revealed an up-regulation in Drp1 and Mfn1 genes mRNA expression in comparison with control group. Treatment with 32μg/ml of melittin was also associated with a rise in mRNA expression of Drp1 and Mfn1 as compared to the control group. Conclusion: The results of this study showed that melittin has anticancer effects on 4T1 cell lines in a dose and time dependent manner and can be a good candidate for further research on breast cancer treatment.

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Psycho-Electrophysiological Benefits of Forest Therapies Focused on Qigong and Walking with Elderly Individuals

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18063004 ◽

2021 ◽

Vol 18 (6) ◽

pp. 3004

Author(s):

Jiyune Yi ◽

Seul Gee Kim ◽

Taegyu Khil ◽

Minja Shin ◽

Jin-Hee You ◽

...

Keyword(s):

Urban Forest ◽

Nervous Activity ◽

Health Problems ◽

The Other ◽

Upper Body ◽

Sympathetic Nervous Activity ◽

Control Group ◽

Beta Band ◽

Elderly Individuals ◽

Beneficial Effects

We developed two distinct forest therapy programs (FTPs) and compared their effects on dementia prevention and related health problems for older adults. One was focused on Qigong practice in the forest (QP) and the other involved active walking in the forest (WP). Both FTPs consisted of twelve 2-h sessions over six weeks and were conducted in an urban forest. We obtained data from 25, 18, and 26 participants aged 65 years or above for the QP, WP, and control groups, respectively. Neuropsychological scores via cognition (MoCA), geriatric depression (GDS) and quality of life (EQ-5D), and electrophysiological variables (electroencephalography, bioimpedance, and heart rate variability) were measured. We analyzed the intervention effects with a generalized linear model. Compared to the control group, the WP group showed benefits in terms of neurocognition (increases in the MoCA score, and alpha and beta band power values in the electroencephalogram), sympathetic nervous activity, and bioimpedance in the lower body. On the other hand, the QP group showed alleviated depression and an increased bioimpedance phase angle in the upper body. In conclusion, both active walking and Qigong in the forest were shown to have distinctive neuropsychological and electrophysiological benefits, and both had beneficial effects in terms of preventing dementia and relieving related health problems for elderly individuals.

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Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis

Scientific Reports ◽

10.1038/s41598-021-86129-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Zihao Li ◽

Mian Wang ◽

Hua Zhong ◽

Xin Huang ◽

Xinyin Wu ◽

...

Keyword(s):

Additive Model ◽

Lectin Pathway ◽

Control Group ◽

Single Nucleotide ◽

Key Enzyme ◽

Healthy Control ◽

Tea Drinking ◽

Mannan Binding Lectin ◽

The Complement System ◽

Negative Interactions

AbstractMannan-binding lectin-associated serine protease-2 (MASP-2) has been reported to play an important role as a key enzyme in the lectin pathway of the complement system. The objectives of our study were to determine whether the single-nucleotide polymorphism (SNPs) of MASP2 and the gene-tea drinking interaction were associated with the susceptibility to TB. In total, 503 patients and 494 healthy controls were contained. Three SNPs (rs12142107, rs12711521, and rs7548659) were genotyped. The association between the SNPs and susceptibility to TB were investigated by conducting multivariate unconditional logistic regression analysis. The gene-tea drinking interactions were analyzed by the additive model of marginal structural linear odds models. Both genotype AC + AA at rs12711521 of MASP2 genes and genotype GT + GG at rs7548659 of MASP2 genes were more prevalent in the TB patient group than the healthy control group (OR: 1.423 and 1.439, respectively, P < 0.05). In addition, The relative excess risk of interaction (RERI) between tea drinking and rs12142107, rs12711521, and rs7548659 of MASP2 genes was found to suggest negative interactions, which reached − 0.2311 (95% confidence interval (CI): − 0.4736, − 0.0113), − 0.7080 (95% CI − 1.3998, − 0.0163), and − 0.5140 (95% CI − 0.8988, − 0.1291), respectively (P < 0.05). Our finding indicated that the SNPs (rs12711521 and rs7548659) of MASP2 were associated with the susceptibility to TB. Furthermore, there were negative interactions between tea drinking and rs12142107, rs12711521, and rs75548659 of MASP2 gene, respectively. Our research provides a basis for studying the pathogenesis and prevention of tuberculosis.

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Antioxidant activity of Lactobacillus plantarum NJAU-01 in an animal model of aging

BMC Microbiology ◽

10.1186/s12866-021-02248-5 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Qingfeng Ge ◽

Bo Yang ◽

Rui Liu ◽

Donglei Jiang ◽

Hai Yu ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Lactobacillus Plantarum ◽

Meat Product ◽

The Other ◽

Oxidative Stress Marker ◽

Control Group ◽

Intragastric Administration ◽

Aging Effects ◽

Sterile Saline

Abstract Background Excessive reactive oxygen species (ROS) can cause serious damage to the human body and may cause various chronic diseases. Studies have found that lactic acid bacteria (LAB) have antioxidant and anti-aging effects, and are important resources for the development of microbial antioxidants. This paper was to explore the potential role of an antioxidant strain, Lactobacillus plantarum NJAU-01 screened from traditional dry-cured meat product Jinhua Ham in regulating D-galactose-induced subacute senescence of mice. A total of 48 specific pathogen free Kun Ming mice (SPF KM mice) were randomly allocated into 6 groups: control group with sterile saline injection, aging group with subcutaneously injection of D-galactose, treatments groups with injection of D-galactose and intragastric administration of 107, 108, and 109 CFU/mL L. plantarum NJAU-01, and positive control group with injection of D-galactose and intragastric administration of 1 mg/mL Vitamin C. Results The results showed that the treatment group of L. plantarum NJAU-01 at 109 CFU/mL showed higher total antioxidant capacity (T-AOC) and the antioxidant enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) than those of the other groups in serum, heart and liver. In contrast, the content of the oxidative stress marker malondialdehyde (MDA) showed lower levels than the other groups (P < 0.05). The antioxidant capacity was improved with the supplement of the increasing concentration of L. plantarum NJAU-01. Conclusions Thus, this study demonstrates that L. plantarum NJAU-01 can alleviate oxidative stress by increasing the activities of enzymes involved in oxidation resistance and decreasing level of lipid oxidation in mice.

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