The short-term degradation of cellulosic pulp in lake water and peat soil: A multi-analytical study from the micro to the molecular level

Diabetes mellitus is associated with a high incidence and poor prognosis of cardiovascular disease. The aim of the present study was to examine the effect of relatively short-term (5 weeks) Type I diabetes on the left ventricle, the right ventricle and the vessel (vein) on the left ventricle of the myocardium at molecular level by FTIR (Fourier-transform infrared) microspectroscopy. The rats were categorized into two groups: control group (for the left ventricle myocardium, n=8; for the right ventricle myocardium, n=9; for the vein, n=9) and streptozotocin-induced diabetic group (for the left ventricle myocardium, n=7; for the right ventricle myocardium, n=9; for the vein, n=8). Two adjacent cross-sections of 9 μm thickness were taken from the ventricles of the hearts in two groups of rats by using a cryotome. The first sections were used for FTIR microspectroscopy measurements. The second serial sections were stained by haematoxylin/eosin for comparative purposes. Diabetes caused an increase in the content of lipids, an alteration in protein profile with a decrease in α-helix and an increase in β-sheet structure as well as an increase in glycogen and glycolipid contents in both ventricles and the vein. Additionally, the collagen content was found to be increased in the vein of the diabetic group. The present study demonstrated that diabetes-induced alterations in the rat heart can be detected by correlating the IR spectral changes with biochemical profiles in detail. The present study for the first time demonstrated the diabetes-induced alterations at molecular level in both ventricle myocardia and the veins in relatively short-term diabetes.

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Molecular Mechanisms of Oxytocin Signaling at the Synaptic Connection

Neural Plasticity ◽

10.1155/2018/4864107 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

Jan Bakos ◽

Annamaria Srancikova ◽

Tomas Havranek ◽

Zuzana Bacova

Keyword(s):

Neurodevelopmental Disorders ◽

Molecular Mechanisms ◽

Neuronal Excitability ◽

Molecular Level ◽

Short Term ◽

Oxytocin Receptors ◽

Potential Therapeutic Intervention ◽

Early Alteration

Aberrant regulation of oxytocin signaling is associated with the etiology of neurodevelopmental disorders. Synaptic dysfunctions in neurodevelopmental disorders are becoming increasingly known, and their pathogenic mechanisms could be a target of potential therapeutic intervention. Therefore, it is important to pay attention to the role of oxytocin and its receptor in synapse structure, function, and neuron connectivity. An early alteration in oxytocin signaling may disturb neuronal maturation and may have short-term and long-term pathological consequences. At the molecular level, neurodevelopmental disorders include alterations in cytoskeletal rearrangement and neuritogenesis resulting in a diversity of synaptopathies. The presence of oxytocin receptors in the presynaptic and postsynaptic membranes and the direct effects of oxytocin on neuronal excitability by regulating the activity of ion channels in the cell membrane implicate that alterations in oxytocin signaling could be involved in synaptopathies. The ability of oxytocin to modulate neurogenesis, synaptic plasticity, and certain parameters of cytoskeletal arrangement is discussed in the present review.

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Short-term effects of dietary bovine milk on fatty acid composition of human milk: A preliminary multi-analytical study

Journal of Chromatography B ◽

10.1016/j.jchromb.2020.122189 ◽

2020 ◽

Vol 1154 ◽

pp. 122189

Author(s):

Adele Cutignano ◽

Francesco Siano ◽

Raffaele Romano ◽

Alessandra Aiello ◽

Fabiana Pizzolongo ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Human Milk ◽

Acid Composition ◽

Analytical Study ◽

Bovine Milk ◽

Short Term ◽

Short Term Effects

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In-Situ Molecular-Level Elucidation of Organofluorine Binding Sites in a Whole Peat Soil

Environmental Science & Technology ◽

10.1021/es3026769 ◽

2012 ◽

Vol 46 (19) ◽

pp. 10508-10513 ◽

Cited By ~ 18

Author(s):

James G. Longstaffe ◽

Denis Courtier-Murias ◽

Ronald Soong ◽

Myrna J. Simpson ◽

Werner E. Maas ◽

...

Keyword(s):

Binding Sites ◽

Molecular Level ◽

Peat Soil

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Speciation and Long- and Short-term Molecular-level Dynamics of Soil Organic Sulfur Studied by X-ray Absorption Near-Edge Structure Spectroscopy

Journal of Environmental Quality ◽

10.2134/jeq2010.0061 ◽

2011 ◽

Vol 40 (3) ◽

pp. 704-718 ◽

Cited By ~ 23

Author(s):

Dawit Solomon ◽

Johannes Lehmann ◽

Katrin Knoth de Zarruk ◽

Julia Dathe ◽

James Kinyangi ◽

...

Keyword(s):

Molecular Level ◽

Organic Sulfur ◽

Short Term ◽

Edge Structure ◽

X Ray ◽

X Ray Absorption

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A test of a simple model to predict short-term changes in the phosphorus concentration in lake water

SIL Proceedings 1922-2010 ◽

10.1080/03680770.1980.11896980 ◽

1981 ◽

Vol 21 (1) ◽

pp. 187-196

Author(s):

E. E. Prepas ◽

F. H. Rigler

Keyword(s):

Simple Model ◽

Lake Water ◽

Phosphorus Concentration ◽

Short Term

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Short-term Sunlight Irradiation of Organic Matter Dissolved in Lake Water Increased its Susceptibility to Subsequent Biooxidation

Acta hydrochimica et hydrobiologica ◽

10.1002/aheh.200390010 ◽

2002 ◽

Vol 30 (56) ◽

pp. 285-292 ◽

Cited By ~ 1

Author(s):

Waldemar Grzybowski

Keyword(s):

Organic Matter ◽

Lake Water ◽

Short Term ◽

Sunlight Irradiation

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Active modulation of Hydrogen bonding by sericin enhances cryopreservation outcomes

10.1101/773721 ◽

2019 ◽

Author(s):

L. Underwood ◽

J. Solocinski ◽

E. Rosiek ◽

Q. Osgood ◽

N. Chakraborty

Keyword(s):

Analytical Study ◽

Molecular Level ◽

Raman Microspectroscopy ◽

Carcinoma Cells ◽

Growth Potential ◽

Scanning Calorimetry ◽

Beneficial Effects ◽

Aqueous Environments

AbstractCryopreservation of cells without any toxicity concerns is a critical step in ensuring successful clinical translation of cell-based technologies. Mitigating the toxicity concerns related to most of the commonly used cryoprotectants including dimethyl sulfoxide (DMSO) is an active area of research in cryobiology. In recent years use of additives including polymeric proteins such has sericin have been explored as an additive to cryoprotectant formulations. In this study the thermophysical effect of addition of sericin was investigated. The effect of presence of sericin on the H-bonding strength was investigated using Raman microspectroscopy and other thermophysical effects were quantified using differential scanning calorimetry (DSC) techniques. Finally, the prospect of using sericin as an additive to cryoprotectant formulation was investigated by monitoring cellular viability and growth following exposure to cryogenic temperatures in hepatocellular carcinoma cells. Results indicate significant improvement in post-thaw viability when sericin is used as an additive to DMSO based formulations. While use of trehalose as an additive has beneficial effects by itself, combined usage of sericin and trehalose as additives did result in an improved overall long-term growth potential of the cells.Statement of SignificanceThis study provides for powerful biophysical understanding of how sericin can be used as an additive for cryoprotectant solutions, which allows storage of biologics at low temperatures. It is desirable to replace current components of cryoprotectant formulation (such as DMSO) due to innate toxicity and metabolic derangements to cells. The ability of sericin to improve cryoprotective solutions was mechanistically characterized by Raman microspectroscopy, which allows for molecular level characterization of the nature of H-bonding in aqueous environments in presence of solution components. Thermodynamic analysis of the cryoprotectant solutions containing sericin was undertaken to quantify the relation between solution composition and cryopreservation outcome. This analytical study provides a basis for designing better cryoprotectants with lower thermophysical injury and higher cellular yields.

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