Distribution, virulence, genotypic characteristics and antibiotic resistance of Listeria monocytogenes isolated over one-year monitoring from two pig slaughterhouses and processing plants and their fresh hams

2021 ◽  
Vol 336 ◽  
pp. 108912
Author(s):  
Gianluca Rugna ◽  
Elena Carra ◽  
Federica Bergamini ◽  
Giuliana Franzini ◽  
Silvia Faccini ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Processing Plants ◽  
One Year ◽  
Genotypic Characteristics

scholarly journals Biocide-Tolerant Listeria monocytogenes Isolates from German Food Production Plants Do Not Show Cross-Resistance to Clinically Relevant Antibiotics

2019 ◽  
Vol 85 (20) ◽  
Author(s):  
A. Roedel ◽  
R. Dieckmann ◽  
H. Brendebach ◽  
J. A. Hammerl ◽  
S. Kleta ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Foodborne Pathogens ◽  
Food Production ◽  
Cross Resistance ◽  
High Genetic Diversity ◽  
Content Type ◽  
Processing Plants ◽  
German Food

ABSTRACT Contamination of food during processing is recognized as a main transmission route of Listeria monocytogenes. To prevent microbial contamination, biocides are widely applied as disinfectants in food processing plants. However, there are concerns about the development of antimicrobial resistance in foodborne pathogens due to widespread biocide usage. In our study, 93 L. monocytogenes isolates from German food production facilities were (i) tested for biocide and antibiotic susceptibility using broth microdilution assays, (ii) analyzed for links between reduced biocide susceptibility and antibiotic resistance, and (iii) characterized by whole-genome sequencing, including the detection of genes coding for biocide tolerance, antibiotic resistance, and other virulence factors. Fifteen L. monocytogenes isolates were tolerant to benzalkonium chloride (BAC), and genes conferring BAC tolerance were found in 13 of them. Antibiotic resistance was not associated with biocide tolerance. BAC-tolerant isolates were assigned to 6 multilocus sequence type (MLST) clonal complexes, and most of them harbored internalin A pseudogenes with premature stop codons or deletions (n = 9). Our study demonstrated a high genetic diversity among the investigated isolates including genotypes that are frequently involved in human infections. Although in vitro adaptation studies to biocides have raised concerns about increasing cross-resistance to antibiotics, our results do not provide evidence for this phenomenon in field isolates. IMPORTANCE Foodborne pathogens such as L. monocytogenes can persist in food production environments for a long time, causing perennial outbreaks. Hence, bacterial pathogens are able to survive cleaning and disinfection procedures. Accordingly, they may be repeatedly exposed to sublethal concentrations of disinfectants, which might result in bacterial adaptation to these biocides. Furthermore, antibiotic coresistance and cross-resistance are known to evolve under biocide selection pressure in vitro. Hence, antimicrobial tolerance seems to play a crucial role in the resilience and persistence of foodborne pathogens in the food chain and might reduce therapeutic options in infectious diseases.

scholarly journals Anaerobic Digestion and Removal of Sulfamethoxazole, Enrofloxacin, Ciprofloxacin and Their Antibiotic Resistance Genes in a Full-Scale Biogas Plant

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 502
Author(s):  
Andrea Visca ◽  
Anna Barra Caracciolo ◽  
Paola Grenni ◽  
Luisa Patrolecco ◽  
Jasmin Rauseo ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Anaerobic Digestion ◽  
Resistance Genes ◽  
Emerging Contaminants ◽  
Antibiotic Resistance Genes ◽  
Animal Manure ◽  
Full Scale ◽  
Antibiotic Residues ◽  
Anaerobic Digesters ◽  
One Year

Anaerobic digestion is one of the best ways to re-use animal manure and agricultural residues, through the production of combustible biogas and digestate. However, the use of antibiotics for preventing and treating animal diseases and, consequently, their residual concentrations in manure, could introduce them into anaerobic digesters. If the digestate is applied as a soil fertilizer, antibiotic residues and/or their corresponding antibiotic resistance genes (ARGs) could reach soil ecosystems. This work investigated three common soil emerging contaminants, i.e., sulfamethoxazole (SMX), ciprofloxacin (CIP), enrofloxacin (ENR), their ARGs sul1, sul2, qnrS, qepA, aac-(6′)-Ib-cr and the mobile genetic element intI1, for one year in a full scale anaerobic plant. Six samplings were performed in line with the 45-day hydraulic retention time (HRT) of the anaerobic plant, by collecting input and output samples. The overall results show both antibiotics and ARGs decreased during the anaerobic digestion process. In particular, SMX was degraded by up to 100%, ENR up to 84% and CIP up to 92%, depending on the sampling time. In a similar way, all ARGs declined significantly (up to 80%) in the digestate samples. This work shows how anaerobic digestion can be a promising practice for lowering antibiotic residues and ARGs in soil.

scholarly journals Complete Genome Sequences of Six Listeria monocytogenes Sequence Type 9 Isolates from Meat Processing Plants in Norway

2018 ◽  
Vol 6 (7) ◽  
Author(s):  
Annette Fagerlund ◽  
Solveig Langsrud ◽  
Birgitte Moen ◽  
Even Heir ◽  
Trond Møretrø
Keyword(s):  
Listeria Monocytogenes ◽  
Complete Genome ◽  
Foodborne Pathogen ◽  
Sequence Type ◽  
Nanopore Sequencing ◽  
Meat Processing ◽  
Sequencing Data ◽  
Genome Sequences ◽  
Fatal Disease ◽  
Processing Plants

ABSTRACT Listeria monocytogenes is a foodborne pathogen that causes the often-fatal disease listeriosis. We present here the complete genome sequences of six L. monocytogenes isolates of sequence type 9 (ST9) collected from two different meat processing facilities in Norway. The genomes were assembled using Illumina and Nanopore sequencing data.

Behavior of Listeria monocytogenes in a Pseudomonas putida Biofilm on a Condensate-Forming Surface

2004 ◽  
Vol 67 (2) ◽  
pp. 322-327 ◽  
Author(s):  
ASHRAF N. HASSAN ◽  
DAWN M. BIRT ◽  
JOSEPH F. FRANK
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Pseudomonas Putida ◽  
Microscopic Observation ◽  
Pure Culture ◽  
Extracellular Polymeric Substances ◽  
Plate Count ◽  
Free Surfaces ◽  
Processing Plants ◽  
Humidity Chamber

Listeria monocytogenes has been isolated from condensate-forming surfaces in food processing plants. The objective of this research was to observe the behavior of L. monocytogenes on condensate-covered stainless steel with a Pseudomonas putida biofilm. L. monocytogenes–containing biofilms, either with or without added chicken protein, were incubated in a high humidity chamber at 12°C to allow formation of condensate. Samples were analyzed for attached and unattached L. monocytogenes and total plate count periodically for 35 days. Samples were also taken for microscopic observation of Listeria and bacterial extracellular polymeric substances (EPS). L. monocytogenes attached in significantly greater numbers (>3-log difference) to surfaces with preexisting P. putida biofilms than to Pseudomonas-free surfaces. L. monocytogenes survived in the presence or absence of P. putida with no added nutrients for 35 days, with numbers of survivors in the range of 3 to 4 log CFU/cm2 in the presence of P. putida and less than 2.9 log CFU/cm2 in pure culture. Attached and unattached L. monocytogenes were at similar levels throughout the incubation under all conditions studied. The addition of protein to the biofilms allowed growth of L. monocytogenes in pure culture during the first 7 days of incubation. Numbers of L. monocytogenes were not affected by the presence of P. putida when protein was present. Unattached L. monocytogenes were at levels of 3.6 to 6.7 log CFU/cm2 on the protein-containing surfaces. Microscopic observation of the condensate-covered biofilms indicated that L. monocytogenes formed microcolonies embedded within an EPS matrix over a 28-day period. This research demonstrates that L. monocytogenes can survive on condensate-forming stainless steel in low and high nutrient conditions, with or without the presence of Pseudomonas biofilm. The Listeria can detach and, therefore, have the potential to contaminate product.

scholarly journals Occurrence of Listeria monocytogenes in Ready-to-Eat Meat Products and Meat Processing Plants in Spain

Foods ◽  
2015 ◽  
Vol 4 (4) ◽  
pp. 271-282 ◽  
Author(s):  
Diego Gómez ◽  
Laura Iguácel ◽  
Mª Rota ◽  
Juan Carramiñana ◽  
Agustín Ariño ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Meat Products ◽  
Meat Processing ◽  
Processing Plants

scholarly journals Cell shape and antibiotic resistance is maintained by the activity of multiple FtsW and RodA enzymes inListeria monocytogenes

2019 ◽  
Author(s):  
Jeanine Rismondo ◽  
Sven Halbedel ◽  
Angelika Gründling
Keyword(s):  
Cell Wall ◽  
Antibiotic Resistance ◽  
Immune System ◽  
Cell Division ◽  
Listeria Monocytogenes ◽  
Protein Complexes ◽  
Inducible Promoter ◽  
Severe Form ◽  
Human Pathogen ◽  
Division Site

AbstractRod-shaped bacteria have two modes of peptidoglycan synthesis: lateral synthesis and synthesis at the cell division site. These two processes are controlled by two macromolecular protein complexes, the elongasome and divisome. Recently, it has been shown that theBacillus subtilisRodA protein, which forms part of the elongasome, has peptidoglycan glycosyltransferase activity. The cell division specific RodA homolog FtsW fulfils a similar role at the divisome. The human pathogenListeria monocytogenesencodes up to six FtsW/RodA homologs, however their functions have not yet been investigated. Analysis of deletion and depletion strains led to the identification of the essential cell division-specific FtsW protein, FtsW1. Interestingly,L. monocytogenesencodes a second FtsW protein, FtsW2, which can compensate for the lack of FtsW1, when expressed from an inducible promoter.L. monocytogenesalso possesses three RodA homologs, RodA1, RodA2 and RodA3 and their combined absence is lethal. Cells of arodA1/rodA3double mutant are shorter and have increased antibiotic and lysozyme sensitivity, probably due to a weakened cell wall. Results from promoter activity assays revealed that expression ofrodA3andftsW2is induced in the presence of antibiotics targeting penicillin binding proteins. Consistent with this, arodA3mutant was more susceptible to the β-lactam antibiotic cefuroxime. Interestingly, overexpression of RodA3 also led to increased cefuroxime sensitivity. Our study highlights thatL. monocytogenesencodes a multitude of functional FtsW and RodA enzymes to produce its rigid cell wall and that their expression needs to be tightly regulated to maintain growth, cell division and antibiotic resistance.ImportanceThe human pathogenListeria monocytogenesis usually treated with high doses of β-lactam antibiotics, often combined with gentamicin. However, these antibiotics only act bacteriostatically onL. monocytogenesand the immune system is needed to clear the infection. Therefore, individuals with a compromised immune system are at risk to develop a severe form ofListeriainfection, which can be fatal in up to 30% of cases. The development of new strategies to treatListeriainfections is therefore necessary. Here we show that the expression of some of the FtsW and RodA enzymes ofL. monocytogenesis induced by the presence of β-lactam antibiotics and their combined absence makes bacteria more susceptible to this class of antibiotics. The development of antimicrobials that inhibit the activity or production of FtsW/RodA enzymes might therefore help to improve the treatment ofListeriainfections and thereby lead to a reduction in mortality.

scholarly journals A global perspective of antibiotic-resistant Listeria monocytogenes prevalence in assorted ready to eat foods: A systematic review

2021 ◽  
pp. 2219-2229
Author(s):  
Prudence Mpundu ◽  
Allan Rabson Mbewe ◽  
John Bwalya Muma ◽  
Wizaso Mwasinga ◽  
Nawa Mukumbuta ◽  
...  
Keyword(s):  
Systematic Review ◽  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Meta Analysis ◽  
Global Perspective ◽  
Valuable Insight ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Pooled Prevalence ◽  
Insight Into

Background and Aim: Listeria monocytogenes in ready-to-eat (RTE) foods remains consistently under-reported globally. Nevertheless, several independent studies conducted to investigate have elucidated the prevalence and antibiotic resistance profiles of L. monocytogenes in RTE-associated foods and their antibiotic resistance profiles. Given the rapid increase in consumption of RTE foods of both animal and plant origin, it is imperative to know the prevalence deductive data focusing on how much of L. monocytogenes is present in RTE foods, which is critical for food safety managers and retailers to assess the possible risk posed to end-users. In addition, valuable insight and another angle to the depth of the problem, we conducted a systematic review and meta-analysis to synthesize available data regarding the prevalence of L. monocytogenes in RTE foods and antibiotic resistance profiles. Materials and Methods: We conducted a meta-analysis study of L. monocytogenes and antibiotic resistance to clinically relevant antibiotics to determine the extent of L. monocytogenes contamination in RTE foods and antibiotic resistance profiles. The primary search terms, also known as keywords used, were restricted to peer-reviewed and review articles, and databases, including Google Scholars, Science-Direct, and Scopus, were searched. The inclusion of articles meeting eligibility criteria published between 2010 and 2020 after title, abstract, and full article screening. Data analysis was performed at multiple stages using quantitative meta-analysis reviews. Results: L. monocytogenes pooled proportion/prevalence was highest in chicken products determined at (22%) followed by various but uncategorized RTE foods at 21%. Regarding antibiotic resistance, profiling's highest pooled prevalence resistance was observed in penicillin at 80% resistance, followed by cephalosporin at 47%. Conclusion: Within its limitations, this study has attempted to provide insight into the pooled proportion/prevalence of L. monocytogenes in RTE foods and the antibiotic resistance profile at the global level. Determining the proportion/ prevalence of L. monocytogenes in RTE foods across the globe and antibiotic resistance profile is essential for providing quality food and reducing public health problems due to unsuccessful treatment of foodborne illness. This study provides insight into the pooled prevalence of L. monocytogenes in RTE foods and the antibiotic resistance profile. The results of this study partly endeavored to help appropriate authorities strengthen their preventive measures on specific RTE foods that are most likely to be contaminated with L. monocytogenes and antibiotic resistance profiles.

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