Identification of α-tubulin, Der f 33, as a novel allergen from Dermatophagoides farinae

The extracts of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) have various therapeutic effects, including inflammation and allergy. In this study, gomisin M2 (GM2) was isolated from S. chinensis and its beneficial effects were assessed against atopic dermatitis (AD). We evaluated the therapeutic effects of GM2 on 2,4-dinitrochlorobenzene (DNCB) and Dermatophagoides farinae extract (DFE)-induced AD-like skin lesions with BALB/c mice ears and within the tumor necrosis factor (TNF)-α and interferon (IFN)-γ-stimulated keratinocytes. The oral administration of GM2 resulted in reduced epidermal and dermal thickness, infiltration of tissue eosinophils, mast cells, and helper T cells in AD-like lesions. GM2 suppressed the expression of IL-1β, IL-4, IL-5, IL-6, IL-12a, and TSLP in ear tissue and the expression of IFN-γ, IL-4, and IL-17A in auricular lymph nodes. GM2 also inhibited STAT1 and NF-κB phosphorylation in DNCB/DFE-induced AD-like lesions. The oral administration of GM2 reduced levels of IgE (DFE-specific and total) and IgG2a in the mice sera, as well as protein levels of IL-4, IL-6, and TSLP in ear tissues. In TNF-α/IFN-γ-stimulated keratinocytes, GM2 significantly inhibited IL-1β, IL-6, CXCL8, and CCL22 through the suppression of STAT1 phosphorylation and the nuclear translocation of NF-κB. Taken together, these results indicate that GM2 is a biologically active compound that exhibits inhibitory effects on skin inflammation and suggests that GM2 might serve as a remedy in inflammatory skin diseases, specifically on AD.

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Effect of Co-Inoculation of Bacillus sp. Strain with Bacterial Endophytes on Plant Growth and Colonization in Tomato Plant (Solanum lycopersicum)

Microbiology Research ◽

10.3390/microbiolres12020032 ◽

2021 ◽

Vol 12 (2) ◽

pp. 480-490

Author(s):

Ahsanul Salehin ◽

Ramesh Raj Puri ◽

Md Hafizur Rahman Hafiz ◽

Kazuhito Itoh

Keyword(s):

Plant Growth ◽

Solanum Lycopersicum ◽

Plant Growth Promotion ◽

Tomato Plant ◽

Growth Promotion ◽

Growth Parameters ◽

The Other ◽

Time Interval ◽

Bacillus Sp ◽

F 33

Colonization of a biofertilizer Bacillus sp. OYK strain, which was isolated from a soil, was compared with three rhizospheric and endophytic Bacillus sp. strains to evaluate the colonization potential of the Bacillus sp. strains with a different origin. Surface-sterilized seeds of tomato (Solanum lycopersicum L. cv. Chika) were sown in the sterilized vermiculite, and four Bacillus sp. strains were each inoculated onto the seed zone. After cultivation in a phytotron, plant growth parameters and populations of the inoculants in the root, shoot, and rhizosphere were determined. In addition, effects of co-inoculation and time interval inoculation of Bacillus sp. F-33 with the other endophytes were examined. All Bacillus sp. strains promoted plant growth except for Bacillus sp. RF-37, and populations of the rhizospheric and endophytic Bacillus sp. strains were 1.4–2.8 orders higher in the tomato plant than that of Bacillus sp. OYK. The plant growth promotion by Bacillus sp. F-33 was reduced by co-inoculation with the other endophytic strains: Klebsiella sp. Sal 1, Enterobacter sp. Sal 3, and Herbaspirillum sp. Sal 6., though the population of Bacillus sp. F-33 maintained or slightly decreased. When Klebsiella sp. Sal 1 was inoculated after Bacillus sp. F-33, the plant growth-promoting effects by Bacillus sp. F-33 were reduced without a reduction of its population, while when Bacillus sp. F-33 was inoculated after Klebsiella sp. Sal 1, the effects were increased in spite of the reduction of its population. Klebsiella sp. Sal 1 colonized dominantly under both conditions. The higher population of rhizospheric and endophytic Bacillus sp. in the plant suggests the importance of the origin of the strains for their colonization. The plant growth promotion and colonization potentials were independently affected by the co-existing microorganisms.

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Specific suppression of antigen-antibody reactions by a dialysate from Dermatophagoides farinae.

Journal of Pharmacobio-Dynamics ◽

10.1248/bpb1978.10.104 ◽

1987 ◽

Vol 10 (2) ◽

pp. 104-111 ◽

Cited By ~ 8

Author(s):

AKIHIDE KODA ◽

NAOKI INAGAKI ◽

NOBUO TSURUOKA ◽

MICHIO DAIKOKU ◽

HIROICHI NAGAI ◽

...

Keyword(s):

Dermatophagoides Farinae ◽

Specific Suppression ◽

Antigen Antibody

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Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652010000400017 ◽

2010 ◽

Vol 82 (4) ◽

pp. 941-951 ◽

Cited By ~ 2

Author(s):

Cui Yu-bao ◽

Ying Zhou ◽

Shi Weihong ◽

Ma Guifang ◽

Li Yang ◽

...

Keyword(s):

Large Scale ◽

Alpha Helix ◽

Random Coil ◽

Reference Sequence ◽

Scale Production ◽

Dermatophagoides Farinae ◽

E Coli ◽

Large Scale Production ◽

Solid Foundation ◽

Group 2

To obtain the recombinant group 2 allergen product of Dermatophagoides farinae (Der f 2), the Der f 2 gene was synthesized by RT-PCR. The full-length cDNA comprised 441 nucleotides and was 99.3% identical to the reference sequence (GenBank AB195580). The cDNA was bound to vector pET28a to construct plasmid pET28a(+)-Der f 2, which was transformed into E. coli BL21 and induced by IPTG. SDS-PAGE showed a specific band of about 14kDa in the hole cell lysate. s estiated by chroatography, about 3.86 g of the recobinant product as obtained, which conjugated with serum IgE from asthmatic children. The protein had a signal peptide of 17 amino acids. Its secondary structure comprised an alpha helix (19.86%), an extended strand (30.82%), and a random coil (49.32%). The subcellular localization of this allergen was predicted to be at mitochondria. Furthermore, its function was shown to be associated with an MD-2-related lipid-recognition (ML) domain. The results of this study provide a solid foundation for large-scale production of the allergen for clinical diagnosis and treatent of allergic disorders.

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