Geographical variation in seed germination and biochemical response of milk thistle (Silybum marianum) ecotypes exposed to osmotic and salinity stresses

2020 ◽  
Vol 152 ◽  
pp. 112507
Author(s):  
Hossein Hammami ◽  
Bijan Saadatian ◽  
Seied Amir Hossein Hosseini
2018 ◽  
Vol 64 (3) ◽  
pp. 1-10 ◽  
Author(s):  
Agnieszka Rosińska ◽  
Hanna Dorna ◽  
Dorota Szopińska ◽  
Lidia Irzykowska ◽  
Katarzyna Seidler-Łożykowska

Summary Introduction: : Milk thistle (Silybum marianum (L.) Gaertn.) is an important medicinal plant. Achenes of milk thistle contain sylimarin, protecting liver cells against toxic compounds. Objective: The aim of the research was to find an optimum method of evaluation of milk thistle seed germination. Methods: Ten seed samples were tested. The seeds were germinated: on top of blotter paper, on top of blotter paper after seed disinfection, between pleated blotter paper, in rolled blotter paper and in sand. Germination at the first and final counts, the percentages of abnormal seedlings and dead seeds were determined. The correlation coefficients between seed germination, evaluated with various methods, and seedling emergence were calculated. Moreover, fungi associated with seeds and diseased seedlings were identified. Results: The lowest percentage of normal seedlings was observed after germination on the top of blotter. Highly significant positive correlations were noted between seedling emergence and seed germination at the final count evaluated in rolled paper, between pleated paper and in sand. The fungi from genera: Alternaria, Fusarium, Penicillium, Trichoderma, Ulocladium and Verticillium were frequently identified on seeds and seedlings. Conclusions: Infestation with fungi significantly affected milk thistle seed germination and plant emergence. Germination in rolled blotter paper may be recommended for evaluation of milk thistle seed germination, as the most practical and significantly correlated with seedling emergence.


Weed Science ◽  
1978 ◽  
Vol 26 (4) ◽  
pp. 395-398 ◽  
Author(s):  
J. A. Young ◽  
R. A. Evans ◽  
R. B. Hawkes

The germination of seeds (achenes) of milk thistle [Silybum marianum(L.) Gaertn.] was investigated. One month after harvest, milk thistle seeds had afterripening requirements related to germination temperature that limited germination to 10 to 20 C temperatures. The time required to satisfy afterripening requirements was dependent on germination temperature. Generally the higher the incubation temperature during germination, the longer the afterripening requirement (up to a maximum of 5 months). Once afterripening requirements were satisfied, milk thistle seeds germinated over a temperature range of from 0 to 30 C. Optimum germination occurred with 2 to 15 C 16-h cold periods alternating with 10 to 30 C 8-h warm periods. Emergence of milk thistle seedlings decreased with increased burial depth, but substantial emergence occurred from 8 cm. Germination on the surface of the soil or litter was greatly reduced compared to that with slight soil or litter coverage. Potassium nitrate (KNO3) added to the germination substrate at 1.0 mM enhanced the germination of milk thistle seeds at 2 and 5 C incubation temperatures.


2015 ◽  
Vol 5 (1) ◽  
pp. 538-544
Author(s):  
Ivana Safrankova ◽  
Kolackova P ◽  
Rutivckova G

Milk thistle is grown in the Czech Republic as a medicinal herb; silymarin is isolated from its achenes and used for the production of liver and gallbladder medicine. The quality and content of the active compound is influenced not only by environmental factors, but also by pests and pathogens. The occurrence of pests of milk thistle variety Silyb was observed in two localities during the years 2011 2013. In the year 2011 the mycoflora of seeds of four milk thistle varieties was determined. Representatives of 15 species were isolated from the seeds, most of them saprophytic. 21 fungal species were isolated and identified from milk thistle plants during the vegetation; Septoria silybi among the most important ones. Possibilities of protection of milt thistle against pathogens are discussed.


2011 ◽  
Vol 24 (7) ◽  
pp. 1011-1018 ◽  
Author(s):  
N. Chand ◽  
Din Muhammad ◽  
F. R. Durrani ◽  
M. Subhan Qureshi ◽  
Sahibzada S Ullah

2019 ◽  
pp. 25-38
Author(s):  
M. A. Dorri ◽  
B. Kamkar ◽  
M. Aghdasi ◽  
A. R. Safahani

Milk thistle (Silybum marianum) is used as a medicinal plant in the treatment of liver diseases. In order to investigate germination and seedling growth in S. marianum subjected to NaCl, a three replicated experiment was carried out in a randomized complete block design in the laboratory of Gorgan University of Agricultural Sciences and Natural Resources. Treatments included seven salinity levels (50, 75, 100, 125, 150, 175 and 200 mM) and a control (distilled water). Germination percentage (GP), mean germination time (MGT), seed-ling vigour index (SVI), shoot, root and seedling weight were measured. The effect of salinity levels was significant on GP, SVI, MGT and seedling weight and length (p ≤ 0.01). However, the NaCl concentration effect was not significant on shoot : root length ratio and or shoot : root weight ratios. Results showed that germination decreased when salinity increased, while MGT increased. MGT was 1.75 times higher than in the control at the highest salt concentration. MGT difference was not significant between 75 mM NaCl and con-trol, while it dramatically increased by increasing the NaCl concentration from 150 to 200 mM NaCl. Reduc-tion slope and salt tolerance index (STI) were estimated for germination (0.54 and 231.9) and seedling stage (0.24 and 237.4). According to the results, milk thistle could be considered as a valuable medicinal plant in fairly salinized areas.


2001 ◽  
Vol 7 (3-4) ◽  
Author(s):  
G. Karimzedah ◽  
R. Omidbaigi ◽  
Bakhshai D.

Milk thistle (Silybum marianum (L.) Gaertn) has been used in medicine since ancient times, but it has been cultivated as a medicinal plant only in recent decades. The ripe fruit of milk thistle contains flavonoids, which are used to prepare anti-hepatotoxic drugs. The main purpose of this study was to substantiate the effects of irrigation and row spacing on growth, seed yield and the content of active substances (silybin and silymarin) in milk thistle. The results showed that the suitable amount of irrigation was 20 mm and the appropriate row spacing was 25 cm.  


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