scholarly journals Comparative evaluation of the Bio-Rad Geenius HIV-1/2 Confirmatory Assay and the Bio-Rad Multispot HIV-1/2 Rapid Test as an alternative differentiation assay for CLSI M53 algorithm-I

2013 ◽  
Vol 58 ◽  
pp. e85-e91 ◽  
Author(s):  
L. Malloch ◽  
K. Kadivar ◽  
J. Putz ◽  
P.N. Levett ◽  
J. Tang ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Rapid Test ◽  
Confirmatory Assay ◽  
Hiv 1

scholarly journals Comparative evaluation of the Geenius HIV 1/2 Confirmatory Assay and the HIV-1 and HIV-2 Western blots in the Japanese population

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0198924 ◽  
Author(s):  
Makiko Kondo ◽  
Koji Sudo ◽  
Takako Sano ◽  
Takuya Kawahata ◽  
Ichiro Itoda ◽  
...  
Keyword(s):  
Japanese Population ◽  
Comparative Evaluation ◽  
Western Blots ◽  
Confirmatory Assay ◽  
Hiv 1

scholarly journals Evaluation of the Bio-Rad Geenius HIV 1/2 Assay as Part of a Confirmatory HIV Testing Strategy for Quebec, Canada: Comparison with Western Blot and Inno-Lia Assays

2019 ◽  
Vol 57 (6) ◽  
Author(s):  
Bouchra Serhir ◽  
Céline Desjardins ◽  
Florence Doualla-Bell ◽  
Marc Simard ◽  
Cécile Tremblay ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Western Blot ◽  
Hiv Testing ◽  
Rapid Test ◽  
Testing Strategy ◽  
Blood Samples ◽  
Confirmatory Assay ◽  
Hiv 1 ◽  
Hiv Negative ◽  
Specificity Rate

ABSTRACT The rapid confirmatory Bio-Rad Geenius HIV 1/2 assay was evaluated as an alternative to the HIV-1 Western blot (WB) confirmatory assay. A total of 370 retrospective samples collected from 356 patients were tested. Sensitivity of the Geenius assay to detect HIV-1 and HIV-2 infections was 100% and 97%, respectively, and that of the WB assay was 86% and 39%, respectively. Geenius reduced the number of indeterminate results by 85% and exhibited a differentiation capacity for HIV-1 and HIV-2 of 100% and 89%, respectively. Three of 10 patients presenting with an early HIV infection (1 to 2 weeks before seroconversion by WB) were positive using Geenius. None of the HIV-negative samples were positive using Geenius or WB. However, 7% and 10% of them were indeterminate with Geenius and WB, respectively, leading to a specificity rate of 93% for Geenius and 90% for WB. Ninety cadaveric samples (54 negative, 23 HIV-1 positive, and 3 HIV-1 indeterminate) were tested with Geenius, leading to a sensitivity of 100%, a specificity of 96%, and an indeterminate rate of 4%. Our results indicate that the Bio-Rad Geenius HIV 1/2 rapid test exhibits better sensitivity to detect HIV-1 infections and better performance than WB to confirm and differentiate between HIV-1 and HIV-2 infections. The performance of this new confirmatory assay to detect early infections, to reduce the rate of indeterminate status, and to confirm HIV-1 infection in cadaveric blood samples makes Geenius a potent reliable alternative to the WB.

scholarly journals Chronic and Early Antiretroviral Therapy Impact Human Immunodeficiency Virus (HIV) Serological Assay Sensitivity, Leading to More False-Negative Test Results in HIV Diagnosis

2020 ◽  
Vol 222 (10) ◽  
pp. 1660-1669
Author(s):  
Karolien Stoffels ◽  
Fien Vanroye ◽  
Virginie Mortier ◽  
Laurent Debaisieux ◽  
Marie-Luce Delforge ◽  
...  
Keyword(s):  
False Negative ◽  
Rapid Test ◽  
Undetectable Viral Load ◽  
Test Results ◽  
Negative Test ◽  
Immunodeficiency Virus ◽  
Confirmatory Assay ◽  
Rapid Tests ◽  
Hiv 1 ◽  
False Negative Test

Abstract This retrospective study evaluated the reactivity of 3 human immunodeficiency virus (HIV) confirmatory assays (INNO-LIA, Geenius, and MP) and 7 HIV rapid tests on samples from 2 different study populations in Belgium. For the early-treated cohort (83 HIV-1 adult patients treated within 3 months after infection), HIV-1 diagnosis was not obtained in at least 1 confirmatory assay in 12.0% (10/83) and in an HIV rapid test in 31.3% (26/83). Confirmation assay sensitivities ranged from 87.5% to 95.2%, whereas rapid test assay sensitivities ranged from 75.9% to 100%. The time to treatment initiation or the length of time on treatment did not have a statistical influence on the probability to obtain a false-negative test result. The fastest reversion was demonstrated after 4 months of treatment. Among the long-term treated cohort (390 HIV-1 patients with ≥ 9 years of undetectable viral load), false-negative test results were found in at least 1 HIV confirmatory assay for 2.1% (8/390) of the patients and in a HIV rapid test for 4.9% (19/390). Confirmation assay sensitivities ranged from 98.1% to 99.5%, whereas rapid test sensitivities ranged from 96.2% to 100%. Longer treatment increased nonreactivity of the HIV rapid tests (P = .033). Undetectable viral load decreases the sensitivities of HIV diagnostic tests, and further monitoring of the performance of serological assays is advised.

scholarly journals Detection of Treponema pallidum (Syphilis) Antibodies among HIV-Infected Individuals and Sexually-active Attendees of Two Health Facilities in Port Harcourt, Nigeria

2020 ◽  
pp. 17-23
Author(s):  
Iheanyi O. Okonko ◽  
Tochi I. Cookey ◽  
Sofiat Adewuyi-Oseni ◽  
Amaka M. Awanye
Keyword(s):  
Treponema Pallidum ◽  
Rapid Test ◽  
Test Strip ◽  
Cross Sectional Study ◽  
Sexually Active ◽  
Cross Sectional ◽  
Port Harcourt ◽  
Serological Reactivity ◽  
Hiv Antibodies ◽  
Hiv 1

Aim: This study reports on the detection of Treponema pallidum (syphilis) antibodies in HIV infected patients in Port Harcourt, Nigeria. Screening for syphilis was carried out to determine the prevalence levels of these infections, as biological markers of risk, modes, and time functions of their transmission. Study Design: Cross-sectional study. Place and Duration of Study: University of Port Harcourt Teaching Hospital (UPTH) and O.B. Lulu Briggs Medical Centre, University of Port Harcourt, both in Port Harcourt, Nigeria, between August 2012 and July 2015. Methods: A total of 100 HIV-infected individuals and 100 sexually-active attendees were recruited for this study. Samples of blood were collected and re-screened for the presence of HIV antibodies using the Determine HIV-1/2 (Alere), HIV ½ Stat-Pak (Chembio), and HIV-1/2/P24/O ELISA kit (Dia.Pro). The same set of samples were screened for Treponema pallidum specific antibodies using the syphilis Ultra Rapid Test Strip (ACON(R), USA) and syphilis rapid strips (Global, USA) following the respective manufacturer's instructions. Results: Among the 200 samples, serological reactivity was detected for syphilis in 3(1.5%). The incidence of syphilis was higher in males (2.0%) than in females (1.0%). Age, sex, and locality did not significantly (P>0.05) influence the rate of syphilis. Conclusion: This study further confirms the presence of syphilis among the population studied. Routine screening of Syphilis among patients is therefore advocated.

scholarly journals Characterization of New HIV Infections among Adults ≥45 Years—New York City, North Carolina, San Francisco, 2011–2013

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S15-S15
Author(s):  
Mary Evans ◽  
Anne Patala ◽  
Ellsworth Campbell ◽  
Emily Westheimer ◽  
Cynthia L Gay ◽  
...  
Keyword(s):  
Older Adults ◽  
Hiv Prevention ◽  
Hiv Infection ◽  
San Francisco ◽  
Acute Infection ◽  
Study Participant ◽  
Rapid Test ◽  
Bivariate Analysis ◽  
Similar Frequency ◽  
Hiv 1

Abstract Background While HIV prevention activities are often focused on younger people, older people can also be at risk for HIV infection. We aimed to characterize HIV transmission in older adults. Methods The STOP study was a multi-site prospective study of persons with acute HIV infection (AHI) from 2011 to 2013. Older adults were defined as ≥45 years and younger persons were 13–44 years. AHI was defined by a negative rapid test but a reactive antigen/antibody or HIV RNA test. We performed bivariate analysis using Pearson’s chi-square and odds ratios to examine associations between older age and transmission characteristics. Among persons with HIV-1 polymerase (pol) sequences, transmission linkages were inferred when the genetic distance between sequences was <1.5% and did not indicate directionality of transmission. Results Among 86,836 participants (median age, 29 years; 75.0% male; 51.8% MSM), HIV infection was diagnosed in 176 (1.46%) of 12,036 older adults compared with 1,150 (1.53%) of 74,800 younger people (P = 0.56). Among HIV-infected persons, AHI was diagnosed in similar proportions of older and younger people (13.1% vs. 12.6%; P = 0.86). Among HIV-infected persons who participated in partner notification (n = 1,326), older adults were less likely to report meeting a sex partner online (11.3% vs. 26.9%; OR 0.52, 95% CI = 0.35–0.78) and were less likely to name ≥2 sex partners (31.5% vs. 46.8%; OR = 0.28, 95% CI = 0.15–0.53) compared with younger people. Among HIV-infected persons with HIV-1 pol sequences (n = 537), similar proportions of older and younger people had viruses that genetically linked with another study participant (15.9% vs. 23.5%; OR 0.62, 95% CI 0.31–1.22) (Figure). Conclusion In this study, older adults had a similar frequency of newly diagnosed HIV infection, acute infection, and genetic linkage compared with younger people, suggesting that increased HIV prevention efforts may be needed in this population. Disclosures All authors: No reported disclosures.

The diagnostic accuracy of the HIV 1/2/subtype O Tri‐line rapid test compared with ELISA: A pilot study

Oral Diseases ◽  
2020 ◽  
Vol 26 (S1) ◽  
pp. 161-164
Author(s):  
Shumani Charlotte Manenzhe ◽  
Sizakele Pride Ngwenya ◽  
Sindisiwe Londiwe Shangase
Keyword(s):  
Pilot Study ◽  
Diagnostic Accuracy ◽  
Rapid Test ◽  
Hiv 1
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