scholarly journals Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

2014 ◽  
Vol 6 ◽  
pp. 384-394 ◽  
Author(s):  
Abraham T. Girgih ◽  
Rong He ◽  
Sunday Malomo ◽  
Marina Offengenden ◽  
Jianping Wu ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Functional Characterization ◽  
Hemp Seed ◽  
L Protein ◽  
Antihypertensive Peptides

scholarly journals Structural and functional characterization of the Severe fever with thrombocytopenia syndrome virus L protein

2020 ◽  
Author(s):  
Dominik Vogel ◽  
Sigurdur Rafn Thorkelsson ◽  
Emmanuelle R. J. Quemin ◽  
Kristina Meier ◽  
Tomas Kouba ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Functional Characterization ◽  
Limiting Factor ◽  
Genome Replication ◽  
Human Pathogens ◽  
Emerging Viruses ◽  
Replication Process ◽  
L Protein ◽  
Severe Fever

ABSTRACTThe Bunyavirales order contains several emerging viruses with high epidemic potential, including Severe fever with thrombocytopenia syndrome virus (SFTSV). The lack of medical countermeasures, such as vaccines and antivirals, is a limiting factor for the containment of any virus outbreak. To develop such antivirals a profound understanding of the viral replication process is essential. The L protein of bunyaviruses is a multi-functional and multi-domain protein performing both virus transcription and genome replication and, therefore, would be an ideal drug target. We established expression and purification procedures for the full-length L protein of SFTSV. By combining single-particle electron-cryo microscopy and X-ray crystallography, we obtained 3D models covering ∼70% of the SFTSV L protein in the apo-conformation including the polymerase core region, the endonuclease and the cap-binding domain. We compared this first L structure of the Phenuiviridae family to the structures of La Crosse peribunyavirus L protein and influenza orthomyxovirus polymerase. Together with a comprehensive biochemical characterization of the distinct functions of SFTSV L protein, this work provides a solid framework for future structural and functional studies of L protein-RNA interactions and the development of antiviral strategies against this group of emerging human pathogens.

Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed (Cannabis sativa L.) Protein Hydrolysates

2011 ◽  
Vol 88 (11) ◽  
pp. 1767-1774 ◽  
Author(s):  
Abraham T. Girgih ◽  
Chibuike C. Udenigwe ◽  
Huan Li ◽  
Abayomi P. Adebiyi ◽  
Rotimi E. Aluko
Keyword(s):  
Enzyme Inhibition ◽  
Cannabis Sativa ◽  
Protein Hydrolysates ◽  
Hemp Seed ◽  
L Protein ◽  
Kinetics Of

scholarly journals Structural and functional characterization of the severe fever with thrombocytopenia syndrome virus L protein

2020 ◽  
Vol 48 (10) ◽  
pp. 5749-5765 ◽  
Author(s):  
Dominik Vogel ◽  
Sigurdur Rafn Thorkelsson ◽  
Emmanuelle R J Quemin ◽  
Kristina Meier ◽  
Tomas Kouba ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Functional Characterization ◽  
Limiting Factor ◽  
Genome Replication ◽  
Human Pathogens ◽  
Emerging Viruses ◽  
Replication Process ◽  
L Protein ◽  
Severe Fever

Abstract The Bunyavirales order contains several emerging viruses with high epidemic potential, including Severe fever with thrombocytopenia syndrome virus (SFTSV). The lack of medical countermeasures, such as vaccines and antivirals, is a limiting factor for the containment of any virus outbreak. To develop such antivirals a profound understanding of the viral replication process is essential. The L protein of bunyaviruses is a multi-functional and multi-domain protein performing both virus transcription and genome replication and, therefore, is an ideal drug target. We established expression and purification procedures for the full-length L protein of SFTSV. By combining single-particle electron cryo-microscopy and X-ray crystallography, we obtained 3D models covering ∼70% of the SFTSV L protein in the apo-conformation including the polymerase core region, the endonuclease and the cap-binding domain. We compared this first L structure of the Phenuiviridae family to the structures of La Crosse peribunyavirus L protein and influenza orthomyxovirus polymerase. Together with a comprehensive biochemical characterization of the distinct functions of SFTSV L protein, this work provides a solid framework for future structural and functional studies of L protein–RNA interactions and the development of antiviral strategies against this group of emerging human pathogens.

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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