A nosocomial outbreak of Serratia marcescens producing inducible Amp C-type beta-lactamase enzyme and carrying antimicrobial resistance genes within a class 1 integron

ABSTRACT The aim of this study was to investigate the phenotypic and genotypic antimicrobial resistance, integrons, and transferability of resistance markers in 243 aerobic bacteria recovered from pork at slaughter in the People's Republic of China. The organisms belonged to 22 genera of gram-negative bacteria (92.2%) and gram-positive bacteria (7.8%). High levels of resistance were detected to tetracycline, trimethoprim-sulfamethoxazole, and ampicillin (36.2 to 54.3%), and lower levels were detected to nitrofurantoin, cefotaxime, gentamicin, ciprofloxacin, and chloramphenicol (7.8 to 29.2%). Across species, genes conferring antimicrobial resistance were observed with the following frequencies: blaTEM, 40.7%; blaCMY-2, 15.2%; blaCTX-M, 11.5%; sul2, 27.2%; sul1, 14.4%; tet(A), 5.4%; tet(L), 5.4%; tet(M), 5.0%; tet(E), 3.7%; tet(C), 3.3%; tet(S), 2.5%; and tet(K), 0.8%. Various antimicrobial resistance genes were found in new carriers: blaTEM in Lactococcus garvieae, Myroides odoratimimus, Aeromonas hydrophila, Staphylococcus sciuri, Raoultella terrigena, Macrococcus caseolyticus, Acinetobacter ursingii, Sphingobacterium sp., and Oceanobacillus sp.; blaCMY-2 in Lactococcus lactis, Klebsiella oxytoca, Serratia marcescens, Acinetobacter baumannii, and Myroides phaeus; tet(L) in M. caseolyticus; sul1 in Vibrio cincinnatiensis; sul2 in Acinetobacter bereziniae, Acinetobacter johnsonii, and V. cincinnatiensis; and the class 1 integron and gene cassette aadA2 in V. cincinnatiensis. Approximately 6.6% of isolates contained class 1 integrons, and one isolate harbored class 2 integrons. Plasmid associated intI1 and androgen receptor–encoding genes were transferred into Escherichia coli J53 and E. coli DH5α by conjugation and transformation experiments, respectively. Our study highlights the importance of aerobic bacteria from pork as reservoirs for antimicrobial resistance genes and mobile genetic elements that can readily be transferred intra- and interspecies.

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Porcine commensalEscherichia coli: A reservoir for class 1 integrons associated with IS26

10.1101/158808 ◽

2017 ◽

Author(s):

Cameron J. Reid ◽

Ethan R. Wyrsch ◽

Piklu Roy Chowdhury ◽

Tiziana Zingali ◽

Michael Liu ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Genomic Analysis ◽

Metal Resistance ◽

Whole Genome Sequence ◽

Class 1 Integron ◽

E Coli ◽

Class 1 Integrons ◽

Antimicrobial Resistance Genes ◽

Class 1

AbstractPorcine faecal waste is a serious environmental pollutant. Carriage of antimicrobial resistance and virulence-associated genes (VAGs) and the zoonotic potential of commensalEscherichia colifrom swine is largely unknown. Furthermore, little is known about the role of commensalE. colias contributors to the mobilisation of antimicrobial resistance genes between food animals and the environment. Here, we report whole genome sequence analysis of 141E. colifrom the faeces of healthy pigs. Most strains belonged to phylogroups A and B1 and carried i) a class 1 integron; ii) VAGs linked with extraintestinal infection in humans; iii) antimicrobial resistance genesblaTEM, aphAl, cmlA, strAB, tet(A)A,dfrA12, dfrA5, sul1, sul2, sul3; iv)IS26;and v) heavy metal resistance genes (merA, cusA, terA). Carriage of the sulphonamide resistance genesul3was notable in this study. The 141 strains belonged to 42 multilocus sequence types, but clonal complex 10 featured prominently. Structurally diverse class 1 integrons that were frequently associated with IS26 carried unique genetic features that were also identified in extraintestinal pathogenicE. coli(ExPEC) from humans. This study provides the first detailed genomic analysis and point of reference for commensalE. coliof porcine origin, facilitating tracking of specific lineages and the mobile resistance genes they carry.Conflict of Interest StatementNone to declare.

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Tn6450, a Novel Multidrug Resistance Transposon Characterized in a Proteus mirabilis Isolate from Chicken in China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02192-17 ◽

2018 ◽

Vol 62 (4) ◽

pp. e02192-17 ◽

Cited By ~ 7

Author(s):

Yan-Peng Chen ◽

Chang-Wei Lei ◽

Ling-Han Kong ◽

Jin-Xin Zeng ◽

Xiu-Zhong Zhang ◽

...

Keyword(s):

Multidrug Resistance ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Proteus Mirabilis ◽

Mobile Elements ◽

Fluoroquinolone Resistance ◽

Class 1 Integron ◽

Content Type ◽

Antimicrobial Resistance Genes ◽

Class 1

ABSTRACT A novel 65.8-kb multidrug resistance transposon, designated Tn6450, was characterized in a Proteus mirabilis isolate from chicken in China. Tn6450 contains 18 different antimicrobial resistance genes, including cephalosporinase gene blaDHA-1 and fluoroquinolone resistance genes qnrA1 and aac(6′)-Ib-cr. It carries a class 1/2 hybrid integron composed of intI2 and a 3′ conserved segment of the class 1 integron. Tn6450 is derived from Tn7 via acquisition of new mobile elements and resistance genes.

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Multidrug resistantAeromonasspp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Letters in Applied Microbiology ◽

10.1111/lam.13138 ◽

2019 ◽

Vol 68 (5) ◽

pp. 370-377 ◽

Cited By ~ 4

Author(s):

S. Hossain ◽

P.S. Dahanayake ◽

B.C.J. De Silva ◽

M.V.K.S. Wickramanayake ◽

S.H.M.P. Wimalasena ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Danio Rerio ◽

Class 1 Integron ◽

Gene Cassettes ◽

Antimicrobial Resistance Genes ◽

Class 1

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Draft Genome Sequence of a Clinical Isolate ofSerratia marcescens, Strain AH0650_Sm1

Genome Announcements ◽

10.1128/genomea.01007-15 ◽

2015 ◽

Vol 3 (5) ◽

Cited By ~ 2

Author(s):

Yu Wan ◽

Claire L. Gorrie ◽

Adam Jenney ◽

Mirjana Mirceta ◽

Kathryn E. Holt

Keyword(s):

Antimicrobial Resistance ◽

Serratia Marcescens ◽

Resistance Genes ◽

Draft Genome ◽

Multidrug Resistant ◽

Resistant Isolate ◽

Beta Lactamase ◽

Multidrug Efflux ◽

Multidrug Efflux Pumps ◽

Antimicrobial Resistance Genes

Serratia marcescensstrain AH0650_Sm1 is a clinical multidrug-resistant isolate from Australia. Here, we report its annotated draft genome comprising 20 contigs. We identified chromosomal antimicrobial resistance genes including atet(41) variant, anaac(6′)-Icvariant,ampC, a metallo-beta-lactamase, and several putative multidrug efflux pumps, as well as a novel prophage.

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Integron types, gene cassettes, antimicrobial resistance genes and plasmids of Shigella sonnei isolates from outbreaks and sporadic cases in Taiwan

Journal of Medical Microbiology ◽

10.1099/jmm.0.022517-0 ◽

2011 ◽

Vol 60 (2) ◽

pp. 197-204 ◽

Cited By ~ 21

Author(s):

Chung-Yu Chang ◽

Po-Liang Lu ◽

Chung-Che Lin ◽

Tsong-Ming Lee ◽

Mei-Yin Tsai ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Shigella Sonnei ◽

Class 1 Integron ◽

Antimicrobial Resistance Genes ◽

Class 1 ◽

Sporadic Cases ◽

Plasmid Size ◽

Multiple Resistance Genes

This study analysed the presence, location and transferability of integrons and antibiotic resistance genes in 103 Shigella sonnei outbreak isolates and in 32 sporadic isolates from Taiwan. Multiple antimicrobial resistance was common in both outbreak (95 %) and sporadic (97 %) isolates. Class 1 integrons were present in 34 outbreak isolates (33 %) and in six sporadic isolates (19 %). This study is the first, to our knowledge, to identify an atypical sul3-associated class 1 integron carrying the estX-psp-aadA2-cmlA-aadA1-qacH cassette array in Shigella. Class 2 integrons carrying the dfr1-sat2-aadA1 cassette array were predominant in outbreak isolates (90 %) but were not present in sporadic isolates. Other antimicrobial resistance genes not associated with integrons were found to encode resistance to ampicillin (bla TEM), chloramphenicol (cat1), sulfonamide (sul2) and tetracycline (tetA and tetB). The most common plasmid size was 130 kb (observed in 43 and 97 % of 1998 outbreak and sporadic isolates, respectively). In conclusion, the plasmid location of resistance genes and horizontal plasmid transfer promote the spread of multiple resistance genes in outbreak and sporadic isolates of S. sonnei.

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Diversity of antimicrobial resistance genes and class-1-integrons in phylogenetically related porcine and human Escherichia coli

Veterinary Microbiology ◽

10.1016/j.vetmic.2012.06.010 ◽

2012 ◽

Vol 160 (3-4) ◽

pp. 403-412 ◽

Cited By ~ 9

Author(s):

Christina Susanne Hölzel ◽

Katrin Susanne Harms ◽

Johann Bauer ◽

Ilse Bauer-Unkauf ◽

Stefan Hörmansdorfer ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Class 1 Integrons ◽

Antimicrobial Resistance Genes ◽

Class 1

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Prevalence and Antimicrobial Resistance of Salmonella in Market Raw Oysters in Taiwan

Journal of Food Protection ◽

10.4315/0362-028x.jfp-16-336 ◽

2017 ◽

Vol 80 (5) ◽

pp. 734-739 ◽

Cited By ~ 4

Author(s):

Yung-Tsun Lo ◽

Chia-Lan Wang ◽

Bai-Hsung Chen ◽

Chung-Wen Hu ◽

Chung-Hsi Chou

Keyword(s):

Antimicrobial Resistance ◽

Health Hazard ◽

Multidrug Resistant ◽

Contamination Rate ◽

Class 1 Integron ◽

Retail Markets ◽

Antimicrobial Resistance Genes ◽

Class 1 ◽

Highly Correlated ◽

Human Health Hazard

ABSTRACT We tested 137 samples of domestic shucked oysters and 114 samples of imported oysters collected from traditional retail markets and supermarkets during 2010 and 2011 in Taiwan for the presence of Salmonella. We obtained a total of 91 Salmonella isolates, representing nine serotypes, from 80 of the domestic samples. We did not find any Salmonella in the imported oysters. The presence of Salmonella contamination tended to be specific to the area from which the oysters were harvested: the Dongshih area had a significantly higher contamination rate (68.8%) than the Budai (20.0%) and Wanggong (9.1%) areas. In addition, the rate of Salmonella contamination was higher in oysters that were packed or sold with water (P < 0.05). The most commonly identified Salmonella serotypes were Saintpaul (26.4%), Newport (22.0%), and Infantis (13.2%). We screened the isolates for susceptibility to nine antimicrobials and compared them genetically by using PCR for the class 1 integron (int1), tetA, tetB, and blaPSE-1 genes. Eighteen isolates (19.8%) were resistant to at least one antimicrobial agent, and the most frequent resistances were those to tetracycline and oxytetracycline (n = 12, 14.3%).We detected the antimicrobial resistance genes int1, tetA, tetB, and blaPSE-1 in 16.5, 26.4, 6.6, and 22.0% of the isolates, respectively. Eleven of the 18 antimicrobial-resistant isolates contained one or two int1 cassettes, suggesting that the presence of int1 is highly correlated with antimicrobial resistance in Salmonella isolates from oysters. The consumption of oysters is increasing in Taiwan, and information related to Salmonella contamination in oysters is rather limited. Our results indicate that raw oyster consumption from retail markets in Taiwan is associated with a human health hazard owing to Salmonella, including multidrug-resistant Salmonella strains.

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