Integron types, gene cassettes, antimicrobial resistance genes and plasmids of Shigella sonnei isolates from outbreaks and sporadic cases in Taiwan

This study analysed the presence, location and transferability of integrons and antibiotic resistance genes in 103 Shigella sonnei outbreak isolates and in 32 sporadic isolates from Taiwan. Multiple antimicrobial resistance was common in both outbreak (95 %) and sporadic (97 %) isolates. Class 1 integrons were present in 34 outbreak isolates (33 %) and in six sporadic isolates (19 %). This study is the first, to our knowledge, to identify an atypical sul3-associated class 1 integron carrying the estX-psp-aadA2-cmlA-aadA1-qacH cassette array in Shigella. Class 2 integrons carrying the dfr1-sat2-aadA1 cassette array were predominant in outbreak isolates (90 %) but were not present in sporadic isolates. Other antimicrobial resistance genes not associated with integrons were found to encode resistance to ampicillin (bla TEM), chloramphenicol (cat1), sulfonamide (sul2) and tetracycline (tetA and tetB). The most common plasmid size was 130 kb (observed in 43 and 97 % of 1998 outbreak and sporadic isolates, respectively). In conclusion, the plasmid location of resistance genes and horizontal plasmid transfer promote the spread of multiple resistance genes in outbreak and sporadic isolates of S. sonnei.

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Distribution of Antimicrobial Resistance Genes and Class 1 Integron Gene Cassette Arrays in Motile Aeromonas spp. Isolated from Goldfish (Carassius auratus)

Microbial Drug Resistance ◽

10.1089/mdr.2017.0388 ◽

2018 ◽

Vol 24 (8) ◽

pp. 1217-1225 ◽

Cited By ~ 11

Author(s):

Sabrina Hossain ◽

Benthotage Chamara Jayasankha De Silva ◽

Sudu Hakuruge Madusha Pramud Wimalasena ◽

Hansani Nilupama Kumari Senarath Pathirana ◽

Pasan Sepala Dahanayake ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Carassius Auratus ◽

Gene Cassette ◽

Class 1 Integron ◽

Goldfish Carassius Auratus ◽

Antimicrobial Resistance Genes ◽

Class 1

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A nosocomial outbreak of Serratia marcescens producing inducible Amp C-type beta-lactamase enzyme and carrying antimicrobial resistance genes within a class 1 integron

Journal of Hospital Infection ◽

10.1016/j.jhin.2003.07.004 ◽

2004 ◽

Vol 56 (1) ◽

pp. 29-36 ◽

Cited By ~ 19

Author(s):

M Bagattini ◽

M Crispino ◽

F Gentile ◽

E Barretta ◽

D Schiavone ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Serratia Marcescens ◽

Resistance Genes ◽

Beta Lactamase ◽

Nosocomial Outbreak ◽

Class 1 Integron ◽

Antimicrobial Resistance Genes ◽

Class 1

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Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug‐resistant motileAeromonassp. isolated from ornamental guppy (Poecilia reticulata)

Letters in Applied Microbiology ◽

10.1111/lam.13162 ◽

2019 ◽

Cited By ~ 3

Author(s):

S. Hossain ◽

B.C.J. De Silva ◽

M.V.K.S. Wickramanayake ◽

P.S. Dahanayake ◽

S.H.M.P. Wimalasena ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Poecilia Reticulata ◽

Multidrug Resistant ◽

Class 1 Integron ◽

Gene Cassettes ◽

Antimicrobial Resistance Genes ◽

Class 1

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Antimicrobial Resistance and Resistance Genes in Aerobic Bacteria Isolated from Pork at Slaughter

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-455 ◽

2016 ◽

Vol 79 (4) ◽

pp. 589-597 ◽

Cited By ~ 8

Author(s):

LILI LI ◽

RIKKE HEIDEMANN OLSEN ◽

LEI YE ◽

HE YAN ◽

QING NIE ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Klebsiella Oxytoca ◽

Gene Cassette ◽

Aerobic Bacteria ◽

Class 1 Integron ◽

Antimicrobial Resistance Genes ◽

Class 1 ◽

Myroides Odoratimimus ◽

Raoultella Terrigena

ABSTRACT The aim of this study was to investigate the phenotypic and genotypic antimicrobial resistance, integrons, and transferability of resistance markers in 243 aerobic bacteria recovered from pork at slaughter in the People's Republic of China. The organisms belonged to 22 genera of gram-negative bacteria (92.2%) and gram-positive bacteria (7.8%). High levels of resistance were detected to tetracycline, trimethoprim-sulfamethoxazole, and ampicillin (36.2 to 54.3%), and lower levels were detected to nitrofurantoin, cefotaxime, gentamicin, ciprofloxacin, and chloramphenicol (7.8 to 29.2%). Across species, genes conferring antimicrobial resistance were observed with the following frequencies: blaTEM, 40.7%; blaCMY-2, 15.2%; blaCTX-M, 11.5%; sul2, 27.2%; sul1, 14.4%; tet(A), 5.4%; tet(L), 5.4%; tet(M), 5.0%; tet(E), 3.7%; tet(C), 3.3%; tet(S), 2.5%; and tet(K), 0.8%. Various antimicrobial resistance genes were found in new carriers: blaTEM in Lactococcus garvieae, Myroides odoratimimus, Aeromonas hydrophila, Staphylococcus sciuri, Raoultella terrigena, Macrococcus caseolyticus, Acinetobacter ursingii, Sphingobacterium sp., and Oceanobacillus sp.; blaCMY-2 in Lactococcus lactis, Klebsiella oxytoca, Serratia marcescens, Acinetobacter baumannii, and Myroides phaeus; tet(L) in M. caseolyticus; sul1 in Vibrio cincinnatiensis; sul2 in Acinetobacter bereziniae, Acinetobacter johnsonii, and V. cincinnatiensis; and the class 1 integron and gene cassette aadA2 in V. cincinnatiensis. Approximately 6.6% of isolates contained class 1 integrons, and one isolate harbored class 2 integrons. Plasmid associated intI1 and androgen receptor–encoding genes were transferred into Escherichia coli J53 and E. coli DH5α by conjugation and transformation experiments, respectively. Our study highlights the importance of aerobic bacteria from pork as reservoirs for antimicrobial resistance genes and mobile genetic elements that can readily be transferred intra- and interspecies.

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Porcine commensalEscherichia coli: A reservoir for class 1 integrons associated with IS26

10.1101/158808 ◽

2017 ◽

Author(s):

Cameron J. Reid ◽

Ethan R. Wyrsch ◽

Piklu Roy Chowdhury ◽

Tiziana Zingali ◽

Michael Liu ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Genomic Analysis ◽

Metal Resistance ◽

Whole Genome Sequence ◽

Class 1 Integron ◽

E Coli ◽

Class 1 Integrons ◽

Antimicrobial Resistance Genes ◽

Class 1

AbstractPorcine faecal waste is a serious environmental pollutant. Carriage of antimicrobial resistance and virulence-associated genes (VAGs) and the zoonotic potential of commensalEscherichia colifrom swine is largely unknown. Furthermore, little is known about the role of commensalE. colias contributors to the mobilisation of antimicrobial resistance genes between food animals and the environment. Here, we report whole genome sequence analysis of 141E. colifrom the faeces of healthy pigs. Most strains belonged to phylogroups A and B1 and carried i) a class 1 integron; ii) VAGs linked with extraintestinal infection in humans; iii) antimicrobial resistance genesblaTEM, aphAl, cmlA, strAB, tet(A)A,dfrA12, dfrA5, sul1, sul2, sul3; iv)IS26;and v) heavy metal resistance genes (merA, cusA, terA). Carriage of the sulphonamide resistance genesul3was notable in this study. The 141 strains belonged to 42 multilocus sequence types, but clonal complex 10 featured prominently. Structurally diverse class 1 integrons that were frequently associated with IS26 carried unique genetic features that were also identified in extraintestinal pathogenicE. coli(ExPEC) from humans. This study provides the first detailed genomic analysis and point of reference for commensalE. coliof porcine origin, facilitating tracking of specific lineages and the mobile resistance genes they carry.Conflict of Interest StatementNone to declare.

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Tn6450, a Novel Multidrug Resistance Transposon Characterized in a Proteus mirabilis Isolate from Chicken in China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02192-17 ◽

2018 ◽

Vol 62 (4) ◽

pp. e02192-17 ◽

Cited By ~ 7

Author(s):

Yan-Peng Chen ◽

Chang-Wei Lei ◽

Ling-Han Kong ◽

Jin-Xin Zeng ◽

Xiu-Zhong Zhang ◽

...

Keyword(s):

Multidrug Resistance ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Proteus Mirabilis ◽

Mobile Elements ◽

Fluoroquinolone Resistance ◽

Class 1 Integron ◽

Content Type ◽

Antimicrobial Resistance Genes ◽

Class 1

ABSTRACT A novel 65.8-kb multidrug resistance transposon, designated Tn6450, was characterized in a Proteus mirabilis isolate from chicken in China. Tn6450 contains 18 different antimicrobial resistance genes, including cephalosporinase gene blaDHA-1 and fluoroquinolone resistance genes qnrA1 and aac(6′)-Ib-cr. It carries a class 1/2 hybrid integron composed of intI2 and a 3′ conserved segment of the class 1 integron. Tn6450 is derived from Tn7 via acquisition of new mobile elements and resistance genes.

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Multidrug resistantAeromonasspp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Letters in Applied Microbiology ◽

10.1111/lam.13138 ◽

2019 ◽

Vol 68 (5) ◽

pp. 370-377 ◽

Cited By ~ 4

Author(s):

S. Hossain ◽

P.S. Dahanayake ◽

B.C.J. De Silva ◽

M.V.K.S. Wickramanayake ◽

S.H.M.P. Wimalasena ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Danio Rerio ◽

Class 1 Integron ◽

Gene Cassettes ◽

Antimicrobial Resistance Genes ◽

Class 1

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Identification and antibiotic resistance profiling of bacterial isolates from septicaemic soft-shelled turtles (Pelodiscus sinensis)

Veterinární Medicína ◽

10.17221/65/2016-vetmed ◽

2017 ◽

Vol 62 (No. 3) ◽

pp. 169-177 ◽

Cited By ~ 13

Author(s):

TH Chung ◽

SW Yi ◽

BS Kim ◽

WI Kim ◽

GW Shin

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Present Report ◽

Antibiotic Resistance Genes ◽

Tetracycline Resistance ◽

Edwardsiella Tarda ◽

Pelodiscus Sinensis ◽

Class 1 Integron ◽

Class 1 ◽

M 14

The present study sought to identify pathogens associated with septicaemia in the Chinese soft-shelled turtle (Pelodiscus sinensis) and to characterise antibiotic resistance in these pathogens. Twenty-three isolates recovered from the livers of diseased soft-shelled turtles were genetically identified as Aeromonas hydrophila (n = 8), A. veronii (n = 3), Citrobacter freundii (n = 4), Morganella morganii (n = 3), Edwardsiella tarda (n = 2), Wohlfahrtiimonas chitiniclastica (n = 1), Chryseobacterium sp. (n = 1), and Comamonas sp. (n = 1). Most isolates (n = 21) were resistant to ampicillin whereas a low percentage of isolates was susceptible to aminoglycosides (amikacin, gentamicin, and tobramycin). PCR assays and sequence analysis revealed the presence of the qnrS2 and blaTEM antibiotic resistance genes in all isolates. The blaDHA-1, blaCTX-M-14 and blaCMY-2 genes were harboured by 17.4% (n = 4), 13.5% (n = 3) and 8.7% (n = 2) of the strains, respectively. One or more tetracycline resistance genes were detected in 60.9% (n = 14) of the isolates. Four isolates (17.4%) harboured single or multiple class 1 integron cassettes. Collectively, a variety of bacterial pathogens were involved in the occurrence of septicaemia in Chinese soft-shelled turtles and most of the isolates had multi-antibiotic resistant phenotypes. To our knowledge, the present report is the first to identify W. chitiniclastica and Comamonas sp. as causes of septicaemia in soft-shelled turtles and the first to identify Aeromonas spp. with blaCTX-M-14 and blaDHA-1 resistance genes.

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Molecular characterisation of antimicrobial resistance and virulence genes in Escherichia coli strains isolated from diarrhoeic and healthy rabbits in Tunisia

World Rabbit Science ◽

10.4995/wrs.2020.10879 ◽

2020 ◽

Vol 28 (2) ◽

pp. 81

Author(s):

Raouia Ben Rhouma ◽

Ahlem Jouini ◽

Amira Klibi ◽

Safa Hamrouni ◽

Aziza Boubaker ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Virulence Genes ◽

Antimicrobial Agents ◽

Antibiotic Resistance Genes ◽

Virulence Gene ◽

Diffusion Method ◽

Class 1 Integron ◽

E Coli ◽

Class 1

The purpose of this study was to identify Escherichia coli isolates in diarrhoeic and healthy rabbits in Tunisia and characterise their virulence and antibiotic resistance genes. In the 2014-2015 period, 60 faecal samples from diarrhoeic and healthy rabbits were collected from different breeding farms in Tunisia. Susceptibility to 14 antimicrobial agents was tested by disc diffusion method and the mechanisms of gene resistance were evaluated using polymerase chain reaction and sequencing methods. Forty E. coli isolates were recovered in selective media. High frequency of resistance to tetracycline (95%) was detected, followed by different levels of resistance to sulphonamide (72.5%), streptomycin (62.5%), trimethoprim-sulfamethoxazole (60%), nalidixic acid (32.5%), ampicillin (37.5%) and ticarcillin (35%). E. coli strains were susceptible to cefotaxime, ceftazidime and imipenem. Different variants of blaTEM, tet, sul genes were detected in most of the strains resistant to ampicillin, tetracycline and sulphonamide, respectively. The presence of class 1 integron was studied in 29 sulphonamide-resistant E. coli strains from which 15 harboured class 1 integron with four different arrangements of gene cassettes, dfrA17+aadA5 (n=9), dfrA1 + aadA1 (n=4), dfrA12 + addA2 (n=1), dfrA12+orf+addA2 (n=1). The qnrB gene was detected in six strains out of 13 quinolone-resistant E. coli strains. Seventeen E. coli isolates from diarrhoeic rabbits harboured the enteropathogenic eae genes associated with different virulence genes tested (fimA, cnf1, aer), and affiliated to B2 (n=8) and D (n=9) phylogroups. Isolated E. coli strains from healthy rabbit were harbouring fim A and/or cnf1 genes and affiliated to A and B1 phylogroups. This study showed that E. coli strains from the intestinal tract of rabbits are resistant to the widely prescribed antibiotics in medicine. Therefore, they constitute a reservoir of antimicrobial-resistant genes, which may play a significant role in the spread of antimicrobial resistance. In addition, the eae virulence gene seemed to be implicated in diarrhoea in breeder rabbits in Tunisia.

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