Improved nanomechanical and in-vitro biocompatibility of graphene oxide-carbon nanotube hydroxyapatite hybrid composites by synergistic effect

2021 ◽  
Vol 117 ◽  
pp. 104376
Author(s):  
Jeevan Jyoti ◽  
Abhimanyu Kiran ◽  
Manjit Sandhu ◽  
Amit Kumar ◽  
Bhanu Pratap Singh ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Carbon Nanotube ◽  
Synergistic Effect ◽  
Hybrid Composites ◽  
In Vitro Biocompatibility

Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering

2018 ◽  
Vol 32 (10) ◽  
pp. 1392-1405 ◽  
Author(s):  
Ali Deniz Dalgic ◽  
Ammar Z. Alshemary ◽  
Ayşen Tezcaner ◽  
Dilek Keskin ◽  
Zafer Evis
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Protein Adsorption ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Osteosarcoma Cell ◽  
In Vitro Biocompatibility ◽  
Microscopy Techniques

In this study, novel graphene oxide–incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(ε-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide. Three-dimensional poly(ε-caprolactone) scaffolds were prepared by wet electrospinning and reinforced with silicate-doped nano-hydroxyapatite/graphene oxide nanocomposite groups to improve bone regeneration potency. Microstructural and chemical characteristics of the scaffolds were investigated by X-ray diffraction, Fourier transform infrared spectroscope and scanning electron microscopy techniques. Protein adsorption and desorption on material surfaces were studied using fetal bovine serum. Presence of graphene oxide in the scaffold, dramatically increased the protein adsorption with decreased desorption. In vitro biocompatibility studies were conducted using human osteosarcoma cell line (Saos-2). Electrospun scaffold group that was prepared with effective concentrations of silicate-doped nano-hydroxyapatite and graphene oxide particles (poly(ε-caprolactone) – 10% silicate-doped nano-hydroxyapatite – 4% graphene oxide) showed improved adhesion, spreading, proliferation and alkaline phosphatase activity compared to other scaffold groups.

Design of a graphene oxide-BODIPY conjugate for glutathione depletion and photodynamic therapy

2D Materials ◽  
2021 ◽  
Author(s):  
Giacomo Reina ◽  
Amalia Ruiz ◽  
Barbara Richichi ◽  
Giacomo Biagiotti ◽  
Gina Elena Giacomoazzo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Photodynamic Therapy ◽  
Cell Viability ◽  
Catalytic Mechanism ◽  
Glutathione Transferase ◽  
Bathochromic Shift ◽  
3D Models ◽  
Glutathione Depletion ◽  
In Vitro Biocompatibility

Abstract Boron dipyrromethene derivates (BODIPYs) are promising photosensitisers (PSs) for cancer treatment using photodynamic therapy (PDT). This study investigates the functionalisation of graphene oxide (GO) with a BODIPY derivate for glutathione (GSH) depletion and PDT. The functionalisation of GO with a 3,5-dichloro-8-(4-boronophenyl) BODIPY via a diol derivatisation with the phenyl boronic acid moiety at the meso position of the BODIPY core, allowed to preserve the intrinsic properties of GO. We demonstrated that both chlorine atoms were substituted by GSH in the presence of glutathione transferase (GST), inducing a relevant bathochromic shift in the absorption/emission features and thus generating the active PS. Ex vitro assessment using cell lysates containing cytoplasmatic GST revealed the intracellular catalytic mechanism for the nucleophilic substitution of the GO-BODIPY adduct with GSH. Confocal microscopy studies showed important differences in the cellular uptake of free BODIPY and GO-BODIPY and revealed the coexistence of GO-BODIPY, GO-BODIPY-GS, and GO-BODIPY-GS2 species inside vesicles and in the cytoplasm of the cells after 24 h of incubation. In vitro biocompatibility and safety of GO and GO-BODIPY were evaluated in 2D and 3D models of prostate adenocarcinoma cells (PC-3), where no toxicity was observed up to 100 µg/mL of GO/GO-BODIPY in all treated groups 24 h post-treatment (cell viability > 90%). Only a slight decrease to 80% at 100 µg/mL was observed after 48 h of incubation. We demonstrated the efficacy of a GO adduct containing an α-chlorine-substituted BODIPY for the simultaneous depletion of intracellular GSH and the photogeneration of reactive oxygen species using a halogen white light source (5.4 mW/cm2) with a maximum in the range of 500-800 nm, which significantly reduced cell viability (< 50%) after irradiation. Our study provides a new vision on how to apply BODIPY derivates and potentiate the toxicity of PDT in prostate and other types of cancer.

Synthesis, Mechanical Properties, and in Vitro Biocompatibility with Osteoblasts of Calcium Silicate–Reduced Graphene Oxide Composites

2014 ◽  
Vol 6 (6) ◽  
pp. 3947-3962 ◽  
Author(s):  
Mehdi Mehrali ◽  
Ehsan Moghaddam ◽  
Seyed Farid Seyed Shirazi ◽  
Saeid Baradaran ◽  
Mohammad Mehrali ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Calcium Silicate ◽  
In Vitro Biocompatibility ◽  
Reduced Graphene ◽  
Oxide Composites

scholarly journals Synergistic effect of CB and GO/CNT hybrid fillers on the mechanical properties and fatigue behavior of NR composites

RSC Advances ◽  
2018 ◽  
Vol 8 (19) ◽  
pp. 10573-10581 ◽  
Author(s):  
Laiyun Wei ◽  
Xuan Fu ◽  
Mingchao Luo ◽  
Zhengtian Xie ◽  
Cheng Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Graphene Oxide ◽  
Carbon Nanotube ◽  
Carbon Black ◽  
Synergistic Effect ◽  
Fatigue Behavior ◽  
Crack Growth Resistance ◽  
Hybrid Fillers ◽  
Superior Mechanical Properties ◽  
Heat Build Up

Graphene oxide (GO) and carbon nanotube (CNT) hybrid fillers were used to replace partial carbon black (CB), and GO/CNT/CB/NR composites were prepared with excellent crack growth resistance, low heat build-up and superior mechanical properties.

Preparation of Ag Doped Hydroxyapatite- Fe3O4-Chitosan Composites: In Vitro Biocompatibility Study on MG-63 Cells for Orthopedic Applications

2018 ◽  
Vol 24 (8) ◽  
pp. 5901-5906
Author(s):  
U Anjaneyulu ◽  
B Priyadarshini ◽  
U Vijayalakshmi
Keyword(s):  
Cell Lines ◽  
Hybrid Composites ◽  
Sol Gel ◽  
Hybrid Nanocomposites ◽  
In Vitro Biocompatibility ◽  
Ball Milling Technique ◽  
Co Precipitation ◽  
Orthopedic Applications ◽  
Biocompatibility Study

Present paper deals with the development of hybrid nanocomposites which combination of Ag doped Hydroxyapatite (Ag:HAP)-Magnetite nanoparticles (Fe3O4NPs) and Chitosan. In this present investigation, we have employed sol–gel method to synthesize Ag:HAP using 5% of Ag concentrations. Furthermore, co-precipitation technique was employed to prepare Fe3O4 NPs and Ag doped HAP was mixed with it to develop hybrid composites. The planetary ball milling technique was used to incorporate the fabricated Ag:HAP-Fe3O4 composite material into the biopolymer chitosan at wt% of 50:25:25 respectively. In Vitro biocompatibility of Ag:HAP-Fe3O4 CS hybrid composites were evaluated by MTT assay using MG-63 cell lines for 24–48 h at 200–1000 μg/ml concentrations. Further, these hybrid composites were characterized by using ATR-FTIR, XRD and SEM techniques. The fabricated hybrid composite was found to be biologically compatible with MG-63 osteoblast cell lines to use in biomedical applications.

scholarly journals Layer-by-layer decorated herbal cell compatible scaffolds for bone tissue engineering: A synergistic effect of graphene oxide and Cissus quadrangularis

2020 ◽  
Vol 35 (1) ◽  
pp. 57-73 ◽  
Author(s):  
Shivaji Kashte ◽  
RK Sharma ◽  
Sachin Kadam
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Synergistic Effect ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cellular Proliferation ◽  
Human Umbilical Cord ◽  
Layer By Layer ◽  
Cissus Quadrangularis

Among various bone regenerative and repair methods, use of osteoinductive scaffold as bone grafts/substitute has gained wide importance worldwide. To develop such osteoinductive scaffold that is more natural and which spontaneously stimulates osteoblast formation without any differentiation media, we prepared electrospun poly ε-caprolactone scaffold which is further modified by means of layer-by-layer method using Cissus quadrangularis callus culture extract and graphene oxide (PCL-GO-CQ). The modified PCL-GO-CQ scaffold was compared with plain poly ε-caprolactone scaffold and poly ε-caprolactone coated only with graphene oxide. Physical properties, such as roughness, wettability, yield strength and tensile strength, of PCL-GO-CQ scaffold were found to be superior. Also, PCL-GO-CQ scaffold showed more in vitro cell compatibility with enhanced cellular proliferation on its surface. Presence of graphene oxide and Cissus quadrangularis callus in scaffold helped in the differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into osteogenic lineage without any differentiation media in less than 20 days. The synergistic effect of Cissus quadrangularis callus extract and graphene oxide in PCL-GO-CQ scaffold enhanced osteoblastic differentiation, osteoconduction and osteoinduction potential of scaffolds making them highly potential in bone regeneration and bone tissue engineering applications.

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