Exploring the sulfate patterns of chondroitin sulfate/dermatan sulfate and keratan sulfate in human pancreatic cancer

2021 ◽  
Vol 205 ◽  
pp. 114339
Author(s):  
Qiang Ren ◽  
Jian Wang ◽  
Chao Liu ◽  
Ling-xin Meng ◽  
Rui-kun Qian ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Chondroitin Sulfate ◽  
Dermatan Sulfate ◽  
Keratan Sulfate ◽  
Human Pancreatic Cancer

scholarly journals Role of keratan sulfate expression in human pancreatic cancer malignancy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Premila D. Leiphrakpam ◽  
Prathamesh P. Patil ◽  
Neeley Remmers ◽  
Benjamin Swanson ◽  
Paul M. Grandgenett ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Keratan Sulfate ◽  
Human Pancreatic Cancer

scholarly journals Contaminants in commercial preparations of ‘purified’ small leucine-rich proteoglycans may distort mechanistic studies

2017 ◽  
Vol 37 (1) ◽  
Author(s):  
Sharon J. Brown ◽  
Heidi R. Fuller ◽  
Philip Jones ◽  
Bruce Caterson ◽  
Sally L. Shirran ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Cell Growth ◽  
Chondroitin Sulfate ◽  
Nerve Cell ◽  
Western Blotting ◽  
Dermatan Sulfate ◽  
Keratan Sulfate ◽  
Mechanistic Studies

The present study reports the perplexing results that came about because of seriously impure commercially available reagents. Commercial reagents and chemicals are routinely ordered by scientists and expected to have been rigorously assessed for their purity. Unfortunately, we found this assumption to be risky. Extensive work was carried out within our laboratory using commercially sourced preparations of the small leucine-rich proteoglycans (SLRPs), decorin and biglycan, to investigate their influence on nerve cell growth. Unusual results compelled us to analyse the composition and purity of both preparations of these proteoglycans (PGs) using both mass spectrometry (MS) and Western blotting, with and without various enzymatic deglycosylations. Commercial ‘decorin’ and ‘biglycan’ were found to contain a mixture of PGs including not only both decorin and biglycan but also fibromodulin and aggrecan. The unexpected effects of ‘decorin’ and ‘biglycan’ on nerve cell growth could be explained by these impurities. Decorin and biglycan contain either chondroitin or dermatan sulfate glycosaminoglycan (GAG) chains whereas fibromodulin only contains keratan sulfate and the large (>2500 kDa), highly glycosylated aggrecan contains both keratan and chondroitin sulfate. The different structure, molecular weight and composition of these impurities significantly affected our work and any conclusions that could be made. These findings beg the question as to whether scientists need to verify the purity of each commercially obtained reagent used in their experiments. The implications of these findings are vast, since the effects of these impurities may already have led to inaccurate conclusions and reports in the literature with concomitant loss of researchers’ funds and time.

scholarly journals Identification of keratan sulfate disaccharide at C-3 position of glucuronate of chondroitin sulfate from Mactra chinensis

2016 ◽  
Vol 473 (22) ◽  
pp. 4145-4158 ◽  
Author(s):  
Kyohei Higashi ◽  
Keita Takeda ◽  
Ann Mukuno ◽  
Yusuke Okamoto ◽  
Sayaka Masuko ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Chondroitin Sulfate ◽  
Hippocampal Neurons ◽  
Uronic Acid ◽  
Dermatan Sulfate ◽  
Biological Activities ◽  
Keratan Sulfate ◽  
Structural Variations ◽  
Low Concentrations ◽  
Mactra Chinensis

Glycosaminoglycans (GAGs), including chondroitin sulfate (CS), dermatan sulfate, heparin, heparan sulfate and keratan sulfate (KS) are linear sulfated repeating disaccharide sequences containing hexosamine and uronic acid [or galactose (Gal) in the case of KS]. Among the GAGs, CS shows structural variations, such as sulfation patterns and fucosylation, which are responsible for their physiological functions through CS interaction with CS-binding proteins. Here, we solved the structure of KS-branched CS-E derived from a clam, Mactra chinensis. KS disaccharide [d-GlcNAc6S-(1→3)-β-d-Gal-(1→] was attached to the C-3 position of GlcA, and consecutive KS-branched disaccharide sequences were found in a CS chain. KS-branched polysaccharides clearly exhibited resistance to degradation by chondroitinase ABC or ACII (at low concentrations) compared with typical CS structures. Furthermore, KS-branched polysaccharides stimulated neurite outgrowth of hippocampal neurons. These results strongly suggest that M. chinensis is a rich source of KS-branched CS, and it has important biological activities.

In Vitro and In Vivo Antitumor Efficacy of Docetaxel and Sorafenib Combination in Human Pancreatic Cancer Cells

2010 ◽  
Vol 999 (999) ◽  
pp. 1-11
Author(s):  
P. Ulivi ◽  
C. Arienti ◽  
W. Zoli ◽  
M. Scarsella ◽  
S. Carloni ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Antitumor Efficacy ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells

scholarly journals Oncogene APOL1 promotes proliferation and inhibits apoptosis via activating NOTCH1 signaling pathway in pancreatic cancer

2021 ◽  
Vol 12 (8) ◽  
Author(s):  
Jiewei Lin ◽  
Zhiwei Xu ◽  
Junjie Xie ◽  
Xiaxing Deng ◽  
Lingxi Jiang ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Signaling Pathway ◽  
Density Lipoprotein ◽  
Human Pancreatic Cancer ◽  
Luciferase Reporter ◽  
Notch1 Signaling ◽  
In Vivo Experiments ◽  
Notch1 Signaling Pathway

AbstractAPOL1 encodes a secreted high-density lipoprotein, which has been considered as an aberrantly expressed gene in multiple cancers. Nevertheless, the role of APOL1 in the regulatory mechanisms of pancreatic cancer remains unknown and should be explored. We identified APOL1 was abnormally elevated in human pancreatic cancer tissues compared with that in adjacent tissues and was associated with poor prognosis. The effects of APOL1 in PC cell proliferation, cell cycle, and apoptosis was verified via functional in vitro and in vivo experiments. The results showed that knockdown of APOL1 significantly inhibited the proliferation and promoted apoptosis of pancreatic cancer. In addition, we identified APOL1 could be a regulator of NOTCH1 signaling pathway using bioinformatics tools, qRT-PCR, dual-luciferase reporter assay, and western blotting. In summary, APOL1 could function as an oncogene to promote proliferation and inhibit apoptosis through activating NOTCH1 signaling pathway expression in pancreatic cancer; therefore, it may act as a novel therapeutic target for pancreatic cancer.

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