Assessing the boron nutritional status by analyzing its cummulative frequency distribution in the hair and whole blood

2018 ◽  
Vol 45 ◽  
pp. 50-56 ◽  
Author(s):  
Juraj Prejac ◽  
Andrey A. Skalny ◽  
Andrei R. Grabeklis ◽  
Suzana Uzun ◽  
Ninoslav Mimica ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Whole Blood

scholarly journals Assessing Human Molybdenum Nutritional Status by Analyzing Frequency distribution Properties in the Hair and Whole Blood with a Median Derivatives Bioassay

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1455-1455
Author(s):  
Berislav Momčilović ◽  
Juraj Prejac ◽  
Ninoslav Mimica
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Whole Blood ◽  
Biological Indicator ◽  
Linear Range ◽  
Linear Segment ◽  
Cross Sectional ◽  
Funding Sources ◽  
Sigmoid Curve ◽  
Ultra Trace

Abstract Objectives Molybdenum is the essential trace element in human nutrition since it is a part of a pterin coenzyme which is essential for the activity of various oxidases. High doses of Mo are neurotoxic to the human brain. The safe adequate intake of Mo is set at 75–200 µg·g−1, but there is no available long-term biological indicator tissue to assess the Mo nutritional status. The aim of this study was to assess human Mo nutritional status with a median derivatives bioassay. Methods We analyzed frequency distribution of hair Mo (HMo) in a prospective, observational, cross sectional and exploratory epidemiological study of 1073 apparently healthy adults (339 men, M and 734 women, W) from Zagreb, CROATIA. Whole blood Mo (WBMo) was assessed in a population subsample of 143 W and 91 M. Both hair and whole blood, a long- and short-term biological indicator, respectively were analyzed for Mo with the ICP MS at the Center for Biotic Medicine, Moscow, Russia: an ISO certified bioelement (electrolytes, trace- and ultra-trace elements) analytical laboratory. Hair Mo natural frequency distribution was analyzed with the aid of median derivatives bioassay logistic sigmoid curve for men and women separately. The range of values below the linear segment of the sigmoid curve (the adequate Mo intake) was considered to reflect Mo deficiency, whereas the values above that linear range indicate Mo dietary excess. The adequate linear range may be further subdivided into marginal (subclinical), true adequate, and ample region with a 30:60:10 ratio, respectively. Results The linear segment of the hair Mo deposition was distinctly different (ng·g−1) and higher in M (34–75) than W (27–57) with the observed median ♀/♂ ratio 0.82. The marginal (subclinical) interval ranges are M 34–46.3, W 27–36). However, there were no difference in the WBMo confidence intervals between M (455–1380) and W (480–1145); the Median ♀/♂ observed ratio is 0.93. Conclusions Adequate human population Mo nutritional status may be accurately assessed by analyzing hair Mo frequency distribution with the median derivatives bioassay. Apparently, there is gender dependent delay of Mo hair saturation in the men hair. For the first time it was possible to differentiate the subclinical levels of sparsely adequate dietary Mo. Funding Sources Institute for Research and Development of Sustainable Eco Systems, 10,000 Zagreb, Croatia.

Assessing lithium nutritional status by analyzing its cumulative frequency distribution in the hair and whole blood

2019 ◽  
Vol 36 (07) ◽  
pp. 113-119
Author(s):  
Ninoslav Mimica ◽  
Juraj Prejac ◽  
Andrey A. Skalny ◽  
Andrei R. Grabeklis ◽  
Berislav Momčilović
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Whole Blood ◽  
Cumulative Frequency ◽  
Cumulative Frequency Distribution

scholarly journals Assessing Human Iron Nutritional Status by Analyzing Its Frequency Distribution Properties in the Hair with a Median Derivatives Bioassay (P10-109-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Berislav Momčilović ◽  
Juraj Prejac ◽  
Ninoslav Mimica
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Whole Blood ◽  
Therapeutic Option ◽  
Human Nutrition ◽  
Similar Amount ◽  
Linear Segment ◽  
Cross Sectional ◽  
Funding Sources ◽  
Sigmoid Curve

Abstract Objectives Iron is the essential trace element (TE) in human nutrition, but also the most frequently observed deficient element in the human nutrition. The aim of this study was to assess human iron nutritional status by assessing its frequency distribution in the hair with a median derivatives bioassay. Methods In this prospective, observational, cross-sectional, exploratory, and epidemiological study we have analyzed hair Fe (HFe) in 1073 apparently healthy adults (339 men M and, 734 women, W) from Zagreb, Croatia. Whole blood iron (WBFe) was assessed in a subsample of this population in 143 Women and 91 Men. Both hair and whole blood samples were analyzed for iron with the ICP MS at the Center for Biotic Medicine, Moscow, Russia. Hair Fe natural frequency distribution was analyzed with the median derivatives bioassay logistic sigmoid curve for men and women separately. The range of values below the linear segment of the sigmoid curve (adequate intake) was considered to reflect Fe deficiency, whereas values above that range indicate Fe dietary excess. Results Women and men have almost identical amount of HFe (median ♂12.72 µg·g-1 vs median ♀ 12.428 µg·g-1). The physiologically adequate HFe linear segment range of the sigmoid curve was 6.09–32.19 µg·g-1 for Men and 6.11 - 28.23 µg·g-1 for Women. Similarly, both women and men have very similar amount of WBFe (median ♂535.6 µg∙g-1 vs median ♀4880 µg∙g-1). The physiologically adequate WBFe linear range of the sigmoid curve was 386.4–606.1 µg∙g-1 for ♀ and 470.4–631.0 µg∙g-1 for ♂. Conclusions Human nutritional status of Fe may be reliably assessed by analyzing its frequency distribution in the hair with median derivatives bioassay. Hair iron is a rare case between the bioelements we have tested thusafar where wholeblood concentrations were higher than that in the hair. Our exploration indicates that the so called iron subclinical deficiency may be adequately quantified. Indeed, continuous subliminal iron supplementation may be a better therapeutic option than current supplementation with high doses associated with the unwanted side effects. Funding Sources Institute for Research and Development of Sustainable Ecosystems, Zagreb, Croatia.

scholarly journals Assessing Human Manganese Nutritional Status by Analyzing Its Frequency Distribution Properties in the Hair with a Median Derivatives Bioassay (P10-110-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Berislav Momčilović ◽  
Juraj Prejac ◽  
Ninoslav Mimica
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Whole Blood ◽  
Biological Indicator ◽  
Linear Range ◽  
Similar Amount ◽  
Linear Segment ◽  
Cross Sectional ◽  
Funding Sources ◽  
Sigmoid Curve

Abstract Objectives Manganese (Mn) is the essential trace element (TE) in human nutrition, but high doses of Mn are toxic to the human mentation and induce brain extrapyramidal system. The main source of manganese is our diet, but there no availale long-term biological indicator for assessing the Mn nutritional status.The aim of this study was to assess human manganese nutritional status by assessing its frequency distribution in the hair with a median derivatives bioassay. Methods In this prospective, observational, cross-sectional, exploratory, and epidemiological study we have analyzed hair Mn (HMn) in 1073 apparently healthy adults (339 men, M and 734 women, W) from Zagreb, Croatia. Whole blood manganese (WBMn) was assessed in a subsample of this population in 143 Women and 91 Men. Both hair and whole blood (short-term biological indicator) samples were analyzed for iron with the ICP MS at the Center for Biotic Medicine, Moscow, Russia: an ISO certified bioelements analytical laboratory. Hair Mn natural frequency distribution was analyzed with the median derivatives bioassay logistic sigmoid curve for men and women separately. The range of values below the linear segment of the sigmoid curve (adequate intake) was considered to reflect Mn deficiency, whereas values above that linear range indicate Mn dietary excess. Results Women and men have almost identical amount of HMn (median ♂0.234 µg·g-1 vs. median ♀ 0.208 µg·g-1). The physiologically adequate HMn linear segment range of the sigmoid curve was 0.090–0.749 µg·g-1 for Men and 0.091–0.628 µg·g-1 for Women. Similarly, both women and men have very similar amount of WBMn (median ♂0.0109 µg∙g-1 vs median ♀0.0118 µg∙g-1). The physiologically adequate WBMn linear range of the sigmoid curve was 0.0084–0.0169 µg∙g-1 for ♀ and 0.0079–0.0179 µg∙g-1 for ♂. Conclusions Human nutritional status of Mn in the population may be reliably assessed by analyzing its frequency distribution in the hair with the median derivatives bioassay. Our exploration indicates how it is possible to diagnose the subclinical states of both manganese nutritional deficiency and excess. When the Mn nutritional status is expressed in a range format it became possible to study simultaneously the subtle and complex multi-bioelement profile interactions within the adequate range of a bioelement intake. Funding Sources Institute for Research and Development of Sustainable Ecosystems, Zagreb, Croatia.

scholarly journals GAMBARAN PENGETAHUAN IBU HAMIL TENTANG KEKURANGAN ENERGI KRONIS (KEK) DI KLATEN

2020 ◽  
Vol 8 (1) ◽  
pp. 48-51
Author(s):  
Isnani Nurhayati ◽  
Anas Rahmad Hidayat ◽  
Tri Hartati
Keyword(s):  
Data Analysis ◽  
Nutritional Status ◽  
Pregnant Women ◽  
Frequency Distribution ◽  
Premature Birth ◽  
Energy Deficiency ◽  
Research Results ◽  
Chronic Energy Deficiency ◽  
Expectant Mothers ◽  
Pregnant Mothers

Background : The need during pregnancy affects the nutritional status of pregnant women due to lack of intake in one of the substances will lead to the need for something impaired nutrients and the need for nutrients that are not constant during gestation. Unbalanced nutritional consumption in pregnant women will result in nutritional problems such as chronic energy deficiency (KEK) that will allow miscarriage, premature birth, BBLR and bleeding after childbirth.Objective : knowing the knowledge of pregnant mothers about chronic energy deficiency in Kebondalem Lor District Puskesmas Klaten Regency.Methods : This research is a descriptive analytic with a crosssectional approach. Population is all expectant mothers in Kebondalem Lor District Puskesmas in Klaten Regency as much as 167 people. Sampling with sample clusters obtained 42 respondents. Instruments using Quesioner contain knowledge of expectant mothers about chronic energy deficiency. Univariic data analysis is the frequency distribution of pregnant women knowledge about KEK, where the research results are conducted interpretation of the data of the question ite m by calculating the answer percentage.Result : From 42 expectant mothers of pregnant women knowledge about chronic energy deficiency are largely a good as much as 18 people (42.9%) and a small portion of respondents 9 people (21.4%) Less knowledgeable.Conclusion : The knowledge of pregnant women about chronic energy deficiency in the area of KebondalemLor District, Klaten Regency most are knowledgeable well.

Quantifying manganese in lymphocytes to assess manganese nutritional status.

1989 ◽  
Vol 35 (9) ◽  
pp. 1939-1941 ◽  
Author(s):  
A Matsuda ◽  
M Kimura ◽  
M Kataoka ◽  
S Ohkuma ◽  
M Sato ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Intravenous Injection ◽  
Whole Blood ◽  
Manganese Concentration ◽  
Dependent Manner ◽  
Dose Dependent ◽  
Better Than ◽  
Manganese Concentrations

Abstract To clarify whether manganese nutritional status is better reflected by the manganese concentration in lymphocytes or in whole blood, we injected manganese solutions intravenously into manganese-deficient rats and determined manganese concentrations in lymphocytes, whole blood, and various tissues. The manganese concentrations in lymphocytes and tissues, but not in whole blood, were significantly less in manganese-deficient rats than in normal rats. These low values could be prevented by intravenous injection of manganese in a dose-dependent manner. These results suggest that, for assessment of manganese nutritional status, measurement of manganese in lymphocytes is better than that in whole blood.

Development and Validation of an ICP-MS Method and Its Application to Determine Multiple Trace Elements in Small Volumes of Whole Blood and Plasma

2020 ◽  
Author(s):  
E M Tanvir ◽  
Karen M Whitfield ◽  
Jack C Ng ◽  
P Nicholas Shaw
Keyword(s):  
Trace Elements ◽  
Nutritional Status ◽  
Trace Element ◽  
Human Blood ◽  
Whole Blood ◽  
Plasma Concentrations ◽  
Essential Elements ◽  
Small Sample ◽  
Plasma Samples ◽  
Element Concentrations

Abstract Essential and nonessential element concentrations in human blood provide important information on the nutritional status of individuals and can assist in the screening or diagnosis of certain disorders and their association with other causative factors. A simple and sensitive method, suitable for use with small sample volumes, for quantification of multiple trace element concentrations in whole blood and plasma has been developed using inductively coupled plasma-mass spectrometry. Method validation was performed using standard reference materials of whole blood and serum using varying sample treatments with nitric acid, water and hydrogen peroxide. The method was applied to quantify the trace element concentrations in whole blood and plasma samples (0.1 mL) from 50 adult blood donors in Queensland. The whole blood sample (5 mL) was collected in Vacutainer tubes with K2EDTA as anticoagulant. The developed method was able to quantify, in blood and plasma samples over a wide range of concentrations, several essential elements: cobalt, copper, zinc, iron, manganese and selenium; the nutritionally probably essential elements vanadium and strontium; and nonessential elements including lead, cadmium, arsenic, caesium, barium, thallium and uranium. Significant differences (P < 0.0001) were observed between whole blood and plasma concentrations for 13 elements; 5 of the measured elements, cobalt (0.49 vs. 0.36 μg/L), copper (1.0 vs. 0.75 mg/L), strontium (28 vs. 16 μg/L), barium (1.5 vs. 0.64 μg/L) and thallium (0.06 vs. 0.03 μg/L), had higher mean concentrations in plasma than in blood. Whole blood concentrations of nine trace elements were significantly correlated (P < 0.0001) with plasma concentrations. The distribution of the trace elements between human blood and plasma varied considerably for the different elements. These results indicate that, using a small sample volume, this assay is suitable for the evaluation of nutritional status as well as in monitoring human toxic elemental exposures.

scholarly journals Whole Blood Transcriptomics Is Relevant to Identify Molecular Changes in Response to Genetic Selection for Feed Efficiency and Nutritional Status in the Pig

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0146550 ◽  
Author(s):  
Maëva Jégou ◽  
Florence Gondret ◽  
Annie Vincent ◽  
Christine Tréfeu ◽  
Hélène Gilbert ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Whole Blood ◽  
Feed Efficiency ◽  
Genetic Selection ◽  
Molecular Changes ◽  
Selection For ◽  
Blood Transcriptomics

scholarly journals Implementation of Hypnobreastfeeding Therapy as an Effort to Reduce the Incidence of Underweight on Children Aged 0–6 Months

2021 ◽  
Vol 9 (E) ◽  
pp. 123-126
Author(s):  
Nur Laily ◽  
Fauzie Rahman ◽  
Andini Octaviana Putri ◽  
Meitria Syahadatina Noor ◽  
Atikah Rahayu ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Exclusive Breastfeeding ◽  
Univariate Analysis ◽  
Test Design ◽  
Mcnemar Test ◽  
Before And After ◽  
Post Test ◽  
Good Attitude

AIM: This study aims to identify implementation of hypnobreastfeeding therapy as an effort to reduce the incidence of underweight on children aged 0–6 months. METHODS: This study is a pre-experimental with pre-test and post-test design. Subjects were children aged 0–6 months who are underweight. The intervention was conducted over 2 months. Data were analyzed by univariate analysis using frequency distribution table and bivariate using McNemar test. RESULTS: Before the education through hypnobreastfeeding therapy, only 33.3% of women having a good attitude about exclusive breastfeeding to children. After the intervention for 2 months, the percentage of good attitude increase as many as 73.3% and the mother is encouraged to continue to breastfeed the child obtained that a total of 18 children (60%) of the total 30 children aged 0–6 months are underweight, which has been entered into the category of normal nutritional status. A difference in the attitude of breastfeeding and status of underweight children aged 0–6 months before and after implementation of hypnobreastfeeding therapy (p < 0.000). CONCLUSION: There are differences in the attitude of the breastfeeding mother (p = 0.012) and underweight status of children aged 0–6 months (p = 0.000) before and after the implementation of hypnobreastfeeding therapy.

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