scholarly journals PC208 Subendothelial Matrix-Targeted Liposomal Nanoparticles for Vascular Therapeutics

2017 ◽  
Vol 65 (6) ◽  
pp. 195S-196S
Author(s):  
Jonathan S. Cudnik ◽  
Michael Best ◽  
Stacy S. Kirkpatrick ◽  
Michael B. Freeman ◽  
Oscar H. Grandas ◽  
...  
Keyword(s):  
Subendothelial Matrix ◽  
Liposomal Nanoparticles

Comparison of the Effect of Adipocyte-derived Stem Cells and Curcumin Nanoliposomes with Phenytoin on Open Cutaneous Wound Healing in Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Mohammadreza Ebrahimzade ◽  
Mohammad Mirdoraghi ◽  
Ameneh Alikarami ◽  
Sahar Heidari ◽  
Tayebeh Rastegar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Tissue ◽  
Healing Time ◽  
Daily Work ◽  
Expression Of Genes ◽  
Skin Ulcers ◽  
Immobility Time ◽  
Liposomal Nanoparticles ◽  
Chromosome Staining

Background: Reducing the healing time of wounds can decrease the patient`s immobility time and their medical costs,leading a faster return of the patients to daily work. Objective: To compare the effect of adipose-derived stem cells and curcumin-containing liposomal nanoparticles with phenytoin on wound healing. Method: After anesthesia of the rats, open skin ulcers were made by a bistoury blade.Subsequently,stem cells were re-moved from the adipose tissue of theupper border of the epididymis. Then,the originality of stem cells was confirmed by the flow cytometry. The fusion method was used to prepare the liposome;and also nanoliposomal particles wereconfirmedby using the DLS microscope.The percentage of recovery and the cell count was measured with IMAGEJ.The expression of genes was assessed by PCR. The number of fibro blasts was counted by immuno histo chemistry techniques.The amount of collagen was determined by Tri-chromosome staining and the number of capillaries was enumerated byH & E staining. Results: The expression of TGF-β1 gene, vascular number, wound healing rate and the numberof fibroblasts increased significantly in adipose tissue-derived stem cells and curcumin nanoliposome groups(p<0.05);the wound surface was also decreased significantly(p<0.05). Conclusion: Based on the results of our research, adipose tissue-derived stem cells and curcumin nanoliposomescan heal wounds efficiently.

Enhanced Antimicrobial Activity of Nisin-Loaded Liposomal Nanoparticles against Foodborne Pathogens

2012 ◽  
Vol 77 (3) ◽  
pp. M165-M170 ◽  
Author(s):  
Yunyun Zou ◽  
Hyeon-Yong Lee ◽  
Yong-Chang Seo ◽  
Juhee Ahn
Keyword(s):  
Antimicrobial Activity ◽  
Foodborne Pathogens ◽  
Liposomal Nanoparticles

scholarly journals Monocyte adhesion to subendothelial components

Blood ◽  
1987 ◽  
Vol 69 (4) ◽  
pp. 1265-1268
Author(s):  
JW Tobias ◽  
MM Bern ◽  
PA Netland ◽  
BR Zetter
Keyword(s):  
Cell Line ◽  
Human Leukocyte ◽  
Adhesion Assay ◽  
U937 Cells ◽  
Monocyte Adhesion ◽  
Active Components ◽  
Dot Blot ◽  
Collagen Types ◽  
Subendothelial Matrix ◽  
Soluble Acid

Human monocytes have been shown to penetrate the endothelial layer of large blood vessels and to adhere to the subendothelial basement membrane. To determine the active components of this process, we have studied the ability of monocytes to adhere to isolated components of the subendothelial matrix. Using a quantitative dot-blot adhesion assay, we find that monocytes adhere preferentially to immobilized laminin and elastin. The monocytes adhere less well to fibronectin and bind poorly or not at all to collagen types I and IV, or to heparan sulfate. Monocyte binding to elastin requires an intact, crosslinked molecule as no binding was observed to soluble, acid-alcohol elastin extracts, to pepsin or elastase digests of elastin, to tropoelastin monomer, or to desmosine/isodesmosine crosslinks. Similar binding profiles to elastin, laminin, and fibronectin were seen with the established human leukocyte cell line U937. The promyelocytic cell line HL60 adhered equally well to laminin but showed slightly reduced adhesion to elastin when compared with the fresh monocytes or U937 cells. Freshly isolated human erythrocytes did not demonstrate significant adhesion to fibronectin, laminin, or elastin.

scholarly journals Subendothelial matrix as the playground for “diabetic milieu” factors in development of angiopathy

2020 ◽  
Vol 36 (4) ◽  
pp. 245-253
Author(s):  
K. A. Nizheradze ◽  
A. I. Khoruzhenko
Keyword(s):  
Subendothelial Matrix

scholarly journals Collagen-dependent platelet dysfunction and its relevance to either mitochondrial ROS or cytosolic superoxide generation: a question about the quality and functional competence of long-stored platelets

2020 ◽  
Author(s):  
Ehteramolsadat Hosseini ◽  
Saba Hojjati ◽  
Safoora Afzalniaye gashti ◽  
Mehran Ghasemzadeh
Keyword(s):  
Platelet Adhesion ◽  
Oxidant Stress ◽  
Ros Generation ◽  
Reverse Correlation ◽  
Platelet Dysfunction ◽  
Mitochondrial Ros ◽  
Ros Accumulation ◽  
The Matrix ◽  
Subendothelial Matrix ◽  
Platelet Spreading

Abstract Background: Upon vascular damage, the exposed subendothelial matrix recruits circulating platelets to site of injury while inducing their firm adhesion mainly via GPVI-collagen interaction. GPVI also supports aggregatory and pro-coagulant functions in arterial shear rate even on the matrix other than collagen. Reactive oxygen species (ROS) modulate these stages of thrombosis; however augmented oxidant stress also disturbs platelet functions. Stored-dependent platelet lesion is associated with the increasing levels of ROS. Whether ROS accumulation is also relevant to collagen-dependent platelet dysfunction is the main interest of this study. Methods: Fresh PRP-PCs (platelet concentrates) were either stimulated with potent ROS-inducers PMA and CCCP or stored for 5 days. Intra-platelet superoxide (O2--) or mitochondrial-ROS and GPVI expression were detected by flowcytometery. GPVI shedding, platelet aggregation and spreading/adhesion to collagen were analyzed by western blot, aggregometry and fluorescence-microscopy respectively. Results: Mitochondrial-ROS levels in 5 days-stored PCs were comparable to those induced by mitochondrial uncoupler, CCCP while O2-- generations were higher than those achieved by PMA. Shedding levels in 5 days-stored PCs were higher than those induced by these potent stimuli. GPVI expressions were reduced comparably in CCCP treated and 5 days-stored PCs. Platelet adhesion was also diminished during storage while demonstrating significant reverse correlation with GPVI shedding. However, only firm adhesion (indicated by spreading or platelet adhesion surface area) was relevant to GPVI expression. Platelet adhesion and aggregation also showed reverse correlations with both O2-- and mitochondrial-ROS formations; nonetheless mitochondrial-ROS was only relevant to firm adhesion. Conclusion: As a sensitive indicator of platelet activation, GPVI shedding correlated with either simple adhesion or spreading to collagen, while GPVI expression was only relevant to platelet spreading. Thereby, if the aim of GPVI evaluation is to examine platelet firm adhesion, expression seems to be a more specific choice. Furthermore, the comparable levels of ROS generation in 5 days-stored PCs and CCCP treated platelets, indicated that these products are significantly affected by oxidative stress. Reverse correlation of accumulating ROS with collagen-dependent platelet dysfunction is also a striking sign of an oxidant-induced lesion that may raise serious question about the post-transfusion quality and competence of longer stored products.

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