Acetylcholinesterase inhibitors neostigmine and physostigmine inhibit induction of alpha-amylase activity during seed germination in barley, Hordeum vulgare var. Jyoti

Life Sciences ◽  
2007 ◽  
Vol 80 (24-25) ◽  
pp. 2386-2388 ◽  
Author(s):  
Veena Beri ◽  
Rajendra Gupta
2008 ◽  
Vol 32 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Renata Braga Souza Lima ◽  
José Francisco de Carvalho Gonçalves ◽  
Silvana Cristina Pando ◽  
Andréia Varmes Fernandes ◽  
André Luis Wendt dos Santos

This study aimed to characterize protein, oil, starch and soluble sugar mobilization as well as the activity of alpha-amylase during rosewood seed germination. Germination test was carried out at 25°C and the following parameters were analyzed: percentage of germination, initial, average, and final germination time. Seed reserve quantification was monitored in quiescent seeds and during different stages of radicle growth. Starch mobilization was studied in function of a-amylase activity. Germination reached 87.5% at the initial, average, and final time of 16, 21 and 30 days, respectively. Oil mobilization showed a negative linear behavior, decreasing 40% between the first and the last stage analyzed, whereas protein levels increased 34.7% during the initial period of germination. Starch content (46.4%) was the highest among those of the metabolites analyzed and starch mobilization occurred inversely to the observed for soluble sugars; alpha-amylase activity increased until the 15th day, a period before radicle emission and corresponding to the highest starch mobilization. The high percentage of rosewood seed germination may be related to the controlled condition used in the germination chamber as well as to high seed reserve mobilization, in special oil and starch.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Jiayi Xing ◽  
Mei Du ◽  
Mingxiu Wang ◽  
...  

AbstractPolygonatum cyrtonema Hua (Huangjing, HJ) has medicinal and edible value in China. However, the seeds of this plant are naturally difficult to germinate. Therefore, to elucidate the mechanism underlying the germination of this plant in order to meet the market demand, the metabolomic and transcriptomic analyses were performed in this study. We observed that plant hormones and α-amylase activity were differentially regulated when comparing germinated and un-germinated seeds. In addition, the metabolites related to phenylpropanoid and flavonoid biosynthesis were significantly up-accumulated in germinated seeds. Hydroxycinnamoyl derivatives and organic acids were observed to be significantly decreased during germination. The results of this study suggested that compared to un-germinated seeds, germinated seeds promote flavonoid synthesis and inhibit lignin synthesis which could be beneficial to the germination of HJ seeds. Furthermore, these results suggested that starch if hydrolyzed into glucose, which could provide the necessary energy for germination. Our results may help to establish a foundation for further research investigating the regulatory networks of seed germination and may facilitate the propagation of HJ seeds.


2015 ◽  
Vol 135 (2) ◽  
pp. AB4
Author(s):  
Andrea A. Pappalardo ◽  
Sherlyana Surja ◽  
Caitlin M. Campion ◽  
Sarah J. Aldrich ◽  
James N. Moy

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
María Dolores Contreras-Aguilar ◽  
Damián Escribano ◽  
María Martín-Cuervo ◽  
Fernando Tecles ◽  
Jose Joaquín Cerón

2021 ◽  
Vol 42 (6supl2) ◽  
pp. 3633-3650
Author(s):  
Matheus Santin Padilha ◽  
◽  
Cileide Maria Medeiros Coelho ◽  
Natalia Carolina Moraes Ehrhardt-Brocardo ◽  
◽  
...  

Seeds with high vigor have greater capacity for hydrolysis and mobilization of stored reserves, which results in the formation of vigorous seedlings, and this behavior is observed under abiotic stress conditions. This study proposes to investigate the relationship of the enzyme alpha-amylase in lots of common-bean seeds with contrasting vigor, when subjected to the absence and presence of salt stress, aiming to identify the relationship of this enzyme with the vigor of the seed lot under these conditions. Seven common-bean cultivars were used. Physiological quality was determined by germination, vigor index and seedling length. The mobilization of reserves was evaluated under absence and presence of salt stress simulated with a NaCl solution with a concentration of 50 mmol L-1. The analyzed variables regarding reserve mobilization were reserve reduction, reserve reduction rate, seedling dry weight, reserve mobilization rate, starch, starch reduction rate and alpha-amylase activity. Results showed that the stress condition negatively affected all the evaluated variables; however, the cultivars classified as having greater vigor showed better physiological performance under stress. Salt stress in common-bean seeds affects seedling performance and reduces alpha-amylase activity during germination, and high-vigor seed lots exhibited higher enzyme activity in the no-stress condition.


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