scholarly journals Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Jiayi Xing ◽  
Mei Du ◽  
Mingxiu Wang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Organic Acids ◽  
Plant Hormones ◽  
Regulatory Networks ◽  
Amylase Activity ◽  
Flavonoid Biosynthesis ◽  
Market Demand ◽  
Potential Mechanism ◽  
Flavonoid Synthesis ◽  
Germinated Seeds

AbstractPolygonatum cyrtonema Hua (Huangjing, HJ) has medicinal and edible value in China. However, the seeds of this plant are naturally difficult to germinate. Therefore, to elucidate the mechanism underlying the germination of this plant in order to meet the market demand, the metabolomic and transcriptomic analyses were performed in this study. We observed that plant hormones and α-amylase activity were differentially regulated when comparing germinated and un-germinated seeds. In addition, the metabolites related to phenylpropanoid and flavonoid biosynthesis were significantly up-accumulated in germinated seeds. Hydroxycinnamoyl derivatives and organic acids were observed to be significantly decreased during germination. The results of this study suggested that compared to un-germinated seeds, germinated seeds promote flavonoid synthesis and inhibit lignin synthesis which could be beneficial to the germination of HJ seeds. Furthermore, these results suggested that starch if hydrolyzed into glucose, which could provide the necessary energy for germination. Our results may help to establish a foundation for further research investigating the regulatory networks of seed germination and may facilitate the propagation of HJ seeds.

scholarly journals Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Jiayi Xing ◽  
Chihong Zhang ◽  
Min Zhou ◽  
...  
Keyword(s):  
Seed Germination ◽  
Plant Hormone ◽  
Amylase Activity ◽  
Sinapic Acid ◽  
Market Demand ◽  
Coumaric Acid ◽  
Potential Mechanism ◽  
Growth Cycles ◽  
Hydrolysis Of ◽  
Germinated Seeds

Abstract Background: Polygonatum cyrtonema Hua is a Chinese traditional medicine in the Liliaceae family. It have unique medical and edible value. However, the Polygonatum cyrtonema seeds are naturally difficult to germinate. Its reproductive and growth cycles should be accelerated through artificial technologies for meeting the market demand. Therefore, to beak the seeds dormancy and to elucidate the mechanism of germination, the metabolomics and transcriptomics analysis were performed in this study. Results: The results indicate that plant hormone (auxin, GA, ABA) were involved in seed germination. Furthermore, the expression levels of unigenes and α-amylase activity were increased in germinated seeds. In addition, the metabolites of phenylpropanoid and flavonoid biosynthesis were accumulated in Polygonatum cyrtonema seeds. Hhydroxycinnamyl (caffeic acid, coniferin and sinapic acid et al.), organic acids (benzoic acid, ferulic acid, and coumaric acid etc.) were significantly decreased, while flavonoids significantly accumulated during the germination. The results suggest that the metabolites of germinated seeds were mainly to promote flavonoids synthesis and to inhibit the lignin synthesis, which was beneficial to germination of Polygonatum cyrtonema. And hydrolysis of starch to glucose can provides necessary energy for germination. Conclusions: Our results give a novel insights into the regulating networks of seed germination and pave the way for Polygonatum cyrtonema seeds propagation and cultivation.

Dormancy-breaking Studies on Echinacea angustifolia

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 456f-457 ◽  
Author(s):  
Ali O. Sari ◽  
Mario R. Morales ◽  
James E. Simon
Keyword(s):  
Native Americans ◽  
Seed Germination ◽  
Tap Water ◽  
The United States ◽  
Market Demand ◽  
Dormancy Breaking ◽  
Echinacea Angustifolia ◽  
Native Populations ◽  
Percent Germination ◽  
Dry Heating

Echinacea is a medicinal plant native to North America. It was used extensively by native Americans in the treatment of their ailments. It is presently one of the most popular medicinal plants in the United States. Its popularity has created a large market demand for the roots and foliage of the plant. The gathering of echinacea from the wild is leading to the reduction of native populations and the destruction of its genetic diversity. Cultivation of medicinal echinaceas is hindered by a low seed germination. Dormancy breaking studies were done on freshly harvested seeds of Echinacea angustifolia. Seed lots were placed under light at a constant temperature of 25 °C and at alternate temperatures of 25/15 °C for 14/10 h, respectively. Germination was more rapid and uniform and percent germination higher at 25 °C than at 25/15 °C. Seed tap-water soaking, dry heating, and sharp heating alteration did not increase germination. The application of 1.0 mM ethephon (2-chloroethylphosphoric acid) increased seed germination to 94% at 25 °C and 86% at 25/15 °C. Untreated seeds gave 65% germination at 25 °C and 11% at 25/15 °C. The application of 2500 mg·L–1 and 3500 mg·L–1 of GA to dry seeds and 2500 mg·L–1 to seeds that have been soaked under tap water and then dried increased germination to 82%, 83%, and 83% at 25 °C and 64%, 78%, and 64% at 25/15 °C, respectively.

Influence of Heat on Seed Germination of Cistus Laurifolius and Cistus Ladanifer

1992 ◽  
Vol 2 (1) ◽  
pp. 15 ◽  
Author(s):  
L Valbuena ◽  
R Tarrega ◽  
E Luis
Keyword(s):  
Seed Germination ◽  
High Temperatures ◽  
Exposure Time ◽  
Germination Rate ◽  
Heat Exposure ◽  
Cistus Ladanifer ◽  
Different Temperatures ◽  
Petri Dishes ◽  
Germinated Seeds

The influence of high temperatures on germination of Cistus laurifolius and Cistus ladanifer seeds was analyzed. Seeds were subjected to different temperatures for different times, afterwards they were sowed in plastic petri dishes and monitored for germinated seeds over two months.The germination rate observed in Cistus ldanifer was greater than in Cistus laurifolius. In both species, heat increased germination percentages. For Cistus laurifolius higher temperatures or longer exposure times were needed. Germination percentages of Cistus ladanifer were lower when heat exposure time was 15 minutes.It must be emphasized that germination occurred when seeds were not treated, while seeds exposed to 150�C for 5 minutes or more did not germinate.

scholarly journals Genome-wide miRNA analysis and integrated network for flavonoid biosynthesis in Osmanthus fragrans

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yong Shi ◽  
Heng Xia ◽  
Xiaoting Cheng ◽  
Libin Zhang
Keyword(s):  
Secondary Metabolites ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Expression Profiles ◽  
Flavonoid Biosynthesis ◽  
Integrated Network ◽  
Osmanthus Fragrans ◽  
Flavonoid Synthesis ◽  
Genome Wide

AbstractBackgroundOsmanthus fragransis an important economical plant containing multiple secondary metabolites including flavonoids and anthocyanins. During the past years, the roles of miRNAs in regulating the biosynthesis of secondary metabolites in plants have been widely investigated. However, few studies on miRNA expression profiles and the potential roles in regulating flavonoid biosynthesis have been reported inO. fragrans.ResultsIn this study, we used high-throughput sequencing technology to analyze the expression profiles of miRNAs in leaf and flower tissues ofO. fragrans. As a result, 106 conserved miRNAs distributed in 47 families and 88 novel miRNAs were identified. Further analysis showed there were 133 miRNAs differentially expressed in leaves and flowers. Additionally, the potential target genes of miRNAs as well as the related metabolic pathways were predicted. In the end, flavonoid content was measured in flower and leaf tissues and potential role of miR858 in regulating flavonoid synthesis was illustrated inO. fragrans.ConclusionsThis study not only provided the genome-wide miRNA profiles in the flower and leaf tissue ofO. fragrans, but also investigated the potential regulatory role of miR858a in flavonoid synthesis inO. fragrans. The results specifically indicated the connection of miRNAs to the regulation of secondary metabolite biosynthesis in non-model economical plant.

scholarly journals Effects of plant hormones and 20-hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedlings growth

2007 ◽  
Vol 6 (24) ◽  
pp. 2792-2802 ◽  
Author(s):  
Bakrim Ahmed ◽  
Lamhamdi Mostapha ◽  
Sayah Fouad ◽  
Chibi Fatiha
Keyword(s):  
Seed Germination ◽  
Plant Hormones ◽  
Lycopersicum Esculentum

scholarly journals Primary metabolite mobilization during germination in rosewood (Aniba rosaeodora Ducke) seeds

2008 ◽  
Vol 32 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Renata Braga Souza Lima ◽  
José Francisco de Carvalho Gonçalves ◽  
Silvana Cristina Pando ◽  
Andréia Varmes Fernandes ◽  
André Luis Wendt dos Santos
Keyword(s):  
Seed Germination ◽  
Amylase Activity ◽  
Starch Content ◽  
Initial Period ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Alpha Amylase ◽  
Primary Metabolite ◽  
Radicle Growth ◽  
Starch Mobilization

This study aimed to characterize protein, oil, starch and soluble sugar mobilization as well as the activity of alpha-amylase during rosewood seed germination. Germination test was carried out at 25°C and the following parameters were analyzed: percentage of germination, initial, average, and final germination time. Seed reserve quantification was monitored in quiescent seeds and during different stages of radicle growth. Starch mobilization was studied in function of a-amylase activity. Germination reached 87.5% at the initial, average, and final time of 16, 21 and 30 days, respectively. Oil mobilization showed a negative linear behavior, decreasing 40% between the first and the last stage analyzed, whereas protein levels increased 34.7% during the initial period of germination. Starch content (46.4%) was the highest among those of the metabolites analyzed and starch mobilization occurred inversely to the observed for soluble sugars; alpha-amylase activity increased until the 15th day, a period before radicle emission and corresponding to the highest starch mobilization. The high percentage of rosewood seed germination may be related to the controlled condition used in the germination chamber as well as to high seed reserve mobilization, in special oil and starch.

Enhancing Biosynthesis of Flavonol Protective Biomaterials Using FLS Transgenic Plants

2017 ◽  
Vol 866 ◽  
pp. 29-32
Author(s):  
Darin Dangrit ◽  
Kanokporn Sompornpailin
Keyword(s):  
Tissue Culture ◽  
Transgenic Plants ◽  
Photosynthetic Pigment ◽  
Flavonoid Biosynthesis ◽  
Carotenoid Pigments ◽  
Chlorophyll B ◽  
Wild Type ◽  
Flavonoid Synthesis ◽  
Transgenic Lines ◽  
Tissue Culture Condition

Flavonol synthase (FLS) gene encodes an enzyme that is involved in conversion substrates into flavonols, quercetin and kaempferol. These substances are a subgroup of flavonoids which have an important role in both plant and human health. Many environmental factors such as temperature, pH and UV-A radiation have been studied and presented relationship with flavonoid synthesis. In this experiment, the combination of visible and UV-A lights was used as factors for elevating flavonoid biosynthesis of wild type (WT) plant and two lines of FLS transgenic plant under tissue culture condition. Both transgenic lines significantly enhanced the accumulation of quercetin and kaempferol substances nearly one fold higher than WT plant did. The photosynthetic pigment levels of chlorophyll A, chlorophyll B and carotenoid in transgenic lines are in the range 45.20-46.88, 16.34-17.04 and 13.63-13.46, while those of WT plants are 35.93, 13.18 and 10.55 (µg/g FW), respectively. Therefore, FLS transgenic plants containing high flavonol content showed a better in the protection photosynthetic pigments by less reductions of chlorophyll and carotenoid pigments.

Water potential of cherry tomato (Lycopersicon esculentum Mill.) placenta and seed germination in response to desiccation during fruit development

2004 ◽  
Vol 14 (2) ◽  
pp. 249-257
Author(s):  
Albert T. Modi ◽  
Belinda J. White
Keyword(s):  
Seed Germination ◽  
Lycopersicon Esculentum ◽  
Water Potential ◽  
Amylase Activity ◽  
Cherry Tomato ◽  
Stages Of Development ◽  
Stage Of Development ◽  
Activity Promotion ◽  
Germination Behaviour ◽  
Seed Water Content

Cherry tomato (Lycopersicon esculentum Mill.) seeds harvested from fruits at four stages of development [2, 4, 6 and 8 weeks after flowering (WAF)] were washed (1% HCl) and germinated after 72 h of desiccation at 20°C, and 76%, 49% or 12% RH. Seed α-amylase activity was determined at each stage of development and correlated with seed germination. Desiccation at 76% and 49% RH had no significant effect on the germination of seeds at 4, 6 and 8 WAF, whereas it improved germination of seeds at 2 WAF. Low RH (12%) significantly reduced seed germination at all stages of development. There was a positive correlation (r = 0.68, P ≤ 0.05) between germination and α-amylase activity during seed development. Scanning electron microscopy also revealed a correlation between starch grain occurrence in the endosperm and α-amylase activity. Placenta water potential decreased with fruit and seed maturation, and correlated negatively with improved seed germination and α-amylase activity. Promotion of seed germination by desiccation in developing cherry tomato seeds was lost 4 weeks prior to mass maturity (80 ± 3% seed water content and –2.3 ± 0.1 MPa placenta water potential). It is proposed that the water potential differential between the placenta and the seeds influences α-amylase activity and germination behaviour during development.

THE INFLUENCE OF THE EXTRACT OF SOME CROPS AND SOIL RESIDUES ON GERMINATION AND GROWTH

1960 ◽  
Vol 40 (1) ◽  
pp. 188-197 ◽  
Author(s):  
K. F. Nielsen ◽  
W. B. Woods ◽  
T. F. Cuddy
Keyword(s):  
Seed Germination ◽  
Root Length ◽  
Deionized Water ◽  
The Other ◽  
Percentage Germination ◽  
Soil Extracts ◽  
Field Crops ◽  
Time Required ◽  
Germination And Growth ◽  
Germinated Seeds

Six plant species were germinated in sand wetted with water extracts of tissues of five field crops and three soils. Deionized water was used as a check f he study was conducted using standard seed germination techniques.Alter 7 to 10 days all of the germinated seeds were harvested and measurements made of the shoot and root lengths.Alfalfa extract caused the greatest reduction in shoot and root length as well as m percentage germination. It caused the greatest increase in the time required for germination. Timothy extract was not quite as harmful as the alfalfa. Extracts of oats, corn and potatoes were still less harmful with potato extract causing the least effect.The soil extracts generally had very little effect when compared with deionized water.Plant species showed marked differences in tolerance to the extracts alfalfa being the most resistant and timothy the least.Where water alone was used rate of germination and per cent germination were as high or higher than with the other extracts, but the root and shoot lengths were not always the greatest.

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