Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent

2021 ◽  
Author(s):  
Huimin Pang ◽  
Jinwen Li ◽  
Zhiwei Miao ◽  
Shu Jie Li
Keyword(s):  
Chondroitin Sulfate ◽  
Amylase Activity ◽  
Alpha Amylase ◽  
Inhibitory Effects ◽  
Hypoglycemic Agent

scholarly journals Inhibition of Pancreatic α-Amylase Activity by a Weight-Loss Herbal Formula RCM-107

2019 ◽  
Author(s):  
Shiqi Luo ◽  
George Lenon ◽  
Harsharn Gill ◽  
Andrew Hung ◽  
Daniel A. Dias ◽  
...  
Keyword(s):  
Weight Loss ◽  
Amylase Activity ◽  
Synergistic Effects ◽  
Glycaemic Index ◽  
Alpha Amylase ◽  
Inhibitory Effects ◽  
Active Compounds ◽  
Residue Contact ◽  
The Individual ◽  
Amylase Inhibitors

Reducing carbohydrates digestion by having low glycaemic index (GI) foods has been linked to weight loss. Inhibiting related enzymes is an alternative way to decrease carbohydrate digestion. RCM-107, an eight-herb formula that is modified from the RCM-104, has indicated significant weight-loss action in clinical trials. However, no research has been conducted to study its effect on the activity of porcine pancreatic alpha-amylase (PPA), which is involved in carbohydrate absorption. In this paper, we used fluorescence PPA inhibition assay to investigate the inhibitory effects of RCM-107 and the individual herbs present in this herbal mixture on amylase activity. Subsequently, molecular docking predicted the key active compounds that may be responsible for the enzyme inhibition. According to our results, both the RCM-107 formula and several individual herbs displayed α-amylase inhibitory effects. Also, marginal synergistic effects of RCM-107 were also detected. In addition, alisol B, (-)-epigallocatechin-3-gallate (EGCG) and plantagoside have been predicted as the key active compounds that may be responsible for the α-amylase inhibition effect of RCM-107 according to inter-residue contact analysis. Finally, Glu233, Gln63, His305, Asp300 and Tyr151 are predicted to be markers of important areas with which potential amylase inhibitors would interact.

Inhaled Salmeterol Induces Salivary Alpha Amylase Activity in Healthy Subjects

2015 ◽  
Vol 135 (2) ◽  
pp. AB4
Author(s):  
Andrea A. Pappalardo ◽  
Sherlyana Surja ◽  
Caitlin M. Campion ◽  
Sarah J. Aldrich ◽  
James N. Moy
Keyword(s):  
Healthy Subjects ◽  
Amylase Activity ◽  
Alpha Amylase ◽  
Salivary Alpha Amylase

scholarly journals Primary metabolite mobilization during germination in rosewood (Aniba rosaeodora Ducke) seeds

2008 ◽  
Vol 32 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Renata Braga Souza Lima ◽  
José Francisco de Carvalho Gonçalves ◽  
Silvana Cristina Pando ◽  
Andréia Varmes Fernandes ◽  
André Luis Wendt dos Santos
Keyword(s):  
Seed Germination ◽  
Amylase Activity ◽  
Starch Content ◽  
Initial Period ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Alpha Amylase ◽  
Primary Metabolite ◽  
Radicle Growth ◽  
Starch Mobilization

This study aimed to characterize protein, oil, starch and soluble sugar mobilization as well as the activity of alpha-amylase during rosewood seed germination. Germination test was carried out at 25°C and the following parameters were analyzed: percentage of germination, initial, average, and final germination time. Seed reserve quantification was monitored in quiescent seeds and during different stages of radicle growth. Starch mobilization was studied in function of a-amylase activity. Germination reached 87.5% at the initial, average, and final time of 16, 21 and 30 days, respectively. Oil mobilization showed a negative linear behavior, decreasing 40% between the first and the last stage analyzed, whereas protein levels increased 34.7% during the initial period of germination. Starch content (46.4%) was the highest among those of the metabolites analyzed and starch mobilization occurred inversely to the observed for soluble sugars; alpha-amylase activity increased until the 15th day, a period before radicle emission and corresponding to the highest starch mobilization. The high percentage of rosewood seed germination may be related to the controlled condition used in the germination chamber as well as to high seed reserve mobilization, in special oil and starch.

scholarly journals Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
María Dolores Contreras-Aguilar ◽  
Damián Escribano ◽  
María Martín-Cuervo ◽  
Fernando Tecles ◽  
Jose Joaquín Cerón
Keyword(s):  
Pilot Study ◽  
Amylase Activity ◽  
Alpha Amylase ◽  
Salivary Alpha Amylase ◽  
Abdominal Disease

scholarly journals Vigor and alpha-amylase activity in common bean seeds under salt stress conditions

2021 ◽  
Vol 42 (6supl2) ◽  
pp. 3633-3650
Author(s):  
Matheus Santin Padilha ◽  
◽  
Cileide Maria Medeiros Coelho ◽  
Natalia Carolina Moraes Ehrhardt-Brocardo ◽  
◽  
...  
Keyword(s):  
Salt Stress ◽  
Common Bean ◽  
Stress Condition ◽  
Amylase Activity ◽  
Reduction Rate ◽  
Stress Conditions ◽  
Alpha Amylase ◽  
Reserve Mobilization ◽  
Relationship Of ◽  
The Relationship

Seeds with high vigor have greater capacity for hydrolysis and mobilization of stored reserves, which results in the formation of vigorous seedlings, and this behavior is observed under abiotic stress conditions. This study proposes to investigate the relationship of the enzyme alpha-amylase in lots of common-bean seeds with contrasting vigor, when subjected to the absence and presence of salt stress, aiming to identify the relationship of this enzyme with the vigor of the seed lot under these conditions. Seven common-bean cultivars were used. Physiological quality was determined by germination, vigor index and seedling length. The mobilization of reserves was evaluated under absence and presence of salt stress simulated with a NaCl solution with a concentration of 50 mmol L-1. The analyzed variables regarding reserve mobilization were reserve reduction, reserve reduction rate, seedling dry weight, reserve mobilization rate, starch, starch reduction rate and alpha-amylase activity. Results showed that the stress condition negatively affected all the evaluated variables; however, the cultivars classified as having greater vigor showed better physiological performance under stress. Salt stress in common-bean seeds affects seedling performance and reduces alpha-amylase activity during germination, and high-vigor seed lots exhibited higher enzyme activity in the no-stress condition.

Inhibitory effects ofCymodocea nodosasulphated polysaccharide onα-amylase activity, liver-kidney toxicities and lipid profile disorders in diabetic rats

2015 ◽  
Vol 121 (5) ◽  
pp. 218-227 ◽  
Author(s):  
Rihab Ben Abdallah Kolsi ◽  
Amel Ben Gara ◽  
Neila Jardak ◽  
Rim Chaaben ◽  
Abdelfattah El Feki ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Amylase Activity ◽  
Diabetic Rats ◽  
Inhibitory Effects

Heterosis, overdominance for grain yield, and alpha-amylase activity in F1 hybrids between near-isogenic Rht dwarf and tall wheats

1997 ◽  
Vol 129 (4) ◽  
pp. 371-378 ◽  
Author(s):  
J. E. FLINTHAM ◽  
W. J. ANGUS ◽  
M. D. GALE
Keyword(s):  
Grain Yield ◽  
Amylase Activity ◽  
Single Gene ◽  
Preharvest Sprouting ◽  
Alpha Amylase ◽  
F1 Hybrids ◽  
Dwarfing Genes ◽  
Heterozygous Condition ◽  
Reduced Height ◽  
Continuous Range

The Rht-B1b, Rht-D1b and Rht-B1c alleles for reduced height in wheat (the Norin 10 and Tom Thumb dwarfing genes previously known as Rht1, Rht2 and Rht3) were exploited in combinations to generate a near-continuous range of plant heights, from 53 cm to 123 cm, amongst near-isogenic homozygotes and F1 hybrids. Pleiotropic yield effects of Rht genes were measured in both homozygous (intravarietal) and heterozygous (intervarietal) genetic backgrounds. Heterosis due to overdominance of Rht genes was detected among intravarietal hybrids. The effects of heterozygosity at other genetic loci (mean dominance) were determined, independently of Rht effects, from comparisons between intravarietal and intervarietal F1 hybrids.Genotypes of intermediate plant heights gave maximum yields, in agreement with other trials of the homozygous lines, so that heterosis (hybrid exceeding best parent) for Rht yield effects was observed in crosses between tall and dwarf isogenic pairs. This heterosis combined additively with increased mean weight per grain in intervarietal crosses, generating the highest overall grain yields in hybrids with semi-dwarf stature in heterozygous genetic backgrounds. The Rht-B1c allele showed single-gene overdominance for grain yield, also the production of alpha-amylase in ripening grains of Maris Huntsman was effectively inhibited in the Rht-B1a/c intravarietal hybrid. The Rht-B1c allele thus offers advantages for both grain yield and grain quality in the heterozygous condition and should be considered as an alternative to the conventional semi-dwarfing genes Rht-B1b and Rht-D1b for F1 varieties in environments conductive to preharvest sprouting.

Enzyme-coupled ultraviolet determination of alpha-amylase activity with the GEMSAEC centrifugal analyzer.

1978 ◽  
Vol 24 (9) ◽  
pp. 1620-1624 ◽  
Author(s):  
W H Porter ◽  
R E Roberts
Keyword(s):  
Linear Response ◽  
Amylase Activity ◽  
Kinetic Studies ◽  
Alpha Amylase ◽  
Coefficients Of Variation ◽  
Endogenous Glucose ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Alpha Glucosidase ◽  
As Effects

Abstract We evaluated the Harleco alpha-glucosidase/hexokinase/glucose-6-phosphate dehydrogenase-coupled alpha-amylase method, bu use of the GEMSAEC centrifugal analyzer. Performance evaluation included kinetic studies of substrate and maltose hydrolysis as well as effects of endogenous glucose and fructose. The reagent was found to give a linear response with alpha-amylase activity to greater than 1200 U/liter. Within-run precision resulted in coefficients of variation (CV) of 0.9 to 3.2% over the range studied. Day-to-day precision corresponded to CV's of 2.4 to 4.4% over the same range of alpha-amylase procedure was found to be good (r = 0.997) for patients' sera examined.

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