Genetic epidemiology of skeletal system aging in apparently healthy human population

AbstractHigh titers of anti-NMDAR1 autoantibodies in brain cause anti-NMDAR1 encephalitis that displays psychiatric symptoms of schizophrenia and/or other psychiatric disorders in addition to neurological symptoms. Low titers of anti-NMDAR1 autoantibodies are reported in the blood of a subset of the general human population and psychiatric patients. Since ∼0.1-0.2% of blood circulating antibodies cross the blood-brain barriers and antibodies can persist for months and years in human blood, it is important to investigate whether chronic presence of these blood circulating anti- NMDAR1 autoantibodies may impair human cognitive functions and contribute to the development of psychiatric symptoms. Here, we generated mice carrying low titers of anti-NMDAR1 autoantibodies in blood against a single antigenic epitope of mouse NMDAR1. Mice carrying the anti-NMDAR1 autoantibodies are healthy and display no differences in locomotion, sensorimotor gating, and contextual memory compared to controls. Chronic presence of the blood circulating anti-NMDAR1 autoantibodies, however, is sufficient to specifically impair T-maze spontaneous alternation in the integrity of blood-brain barriers across all 3 independent mouse cohorts, indicating a robust cognitive deficit in spatial working memory and/or novelty detection. Our studies implicate that chronic presence of low titers of blood circulating anti-NMDAR1 autoantibodies may impair cognitive functions in both the general healthy human population and psychiatric patients.

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Occurrence and significance of Cryptococcus neoformans in the oropharynx and on the skin of a healthy human population

Journal of Clinical Microbiology ◽

10.1128/jcm.6.4.325-327.1977 ◽

1977 ◽

Vol 6 (4) ◽

pp. 325-327 ◽

Cited By ~ 1

Author(s):

H S Randhawa ◽

D K Paliwal

Keyword(s):

Cryptococcus Neoformans ◽

School Children ◽

Human Population ◽

Printing Press ◽

Serological Tests ◽

Healthy Human ◽

Cryptococcal Antigen ◽

Laboratory Workers ◽

Antigen Antibody ◽

Healthy Persons

Cryptococcus neoformans was cultured on one occasion from the oropharyngeal washings in 1 of 820 and from the interdigital areas of the feet in 6 of 723 healthy persons. Among the positive persons were two school children, two laboratory workers, one gardener, one plumber, and one printing press worker. Repeat cultures were negative in all seven positive persons; serological tests for cryptococcal antigen/antibody were negative in three. The results demonstrate that C. neoformans may occur as a transient inhabitant of the oropharynx or skin of healthy people.

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Analysis of inflammatory markers in apparently healthy automobile vehicle drivers in response to exposure to traffic pollution fumes

Pakistan Journal of Medical Sciences ◽

10.12669/pjms.36.4.2025 ◽

2020 ◽

Vol 36 (4) ◽

Author(s):

Hina Riaz Ahmed ◽

Binafsha Manzoor Syed ◽

Zulfiqar Laghari ◽

Suleman Pirzada

Keyword(s):

Inflammatory Markers ◽

Cross Sectional Study ◽

Traffic Pollution ◽

Healthy Human ◽

Cross Sectional ◽

Positive Correlation ◽

Markers Of Inflammation ◽

Tnf Α ◽

Traffic Pollutants ◽

Apparently Healthy

Objective: This study aimed to evaluate pattern of markers of inflammation in apparently healthy drivers who exposed to traffic fumes. Methods: This cross-sectional study was conducted from June 2016 to January 2017 at Liaquat University of Medical & Health Sciences (LUMHS), Jamshoro. It looked into the effects of traffic pollutants on markers of inflammation including CRP, Leukocytes count, IL-6, TNF-α, TNF-β of healthy human volunteers. Eighty-seven, apparently healthy, non-smoking automobile vehicle drivers, having daily contact of traffic exhaust for at least six hours, aged between 18-40 years recruited for this study. Levels of traffic-generated pollutants P.M2.5, P.M10, NOx were recorded in different areas of Hyderabad City. Results: P.M2.5 found to be positively correlated with markers of inflammation including IL-6 (rs = 0.99), TNF-α (rs = 0.41), CRP mg/dl (rs = 0.99) , neutrophils (rs = 0.29), lymphocytes (rs = 0.31), eosinophils (rs = 0.20), monocytes (rs = 0.42) and basophils (rs = 0.16). Positive correlation present among IL-6 (rs = 0.21), TNF-α (rs = 0.49) and CRP mg/dl (rs = 0.22) (rs = -0.31), Leukocytes (rs = 0.14) neutrophils (rs = 0.31), lymphocytes (rs = 0.21), monocytes (rs = 0.50), basophils (rs = 0.17) with P.M10. NOx showed positive correlation with IL-6 (rs = 0.22), TNF-α (rs = 0.48), CRP (rs = 0.22), neutrophils (rs = 0.31), lymphocytes (rs = 0.13), basophils (rs = 0.17) and monocytes (rs = 0.48). Conclusion: Findings of our study suggest that almost all markers of inflammation are positively correlated with traffic pollutants and this condition might raise the risk of systemic diseases. doi: https://doi.org/10.12669/pjms.36.4.2025 How to cite this:Riaz H, Syed BM, Laghari Z, Pirzada S. Analysis of inflammatory markers in apparently healthy automobile vehicle drivers in response to exposure to traffic pollution fumes. Pak J Med Sci. 2020;36(4):---------. doi: https://doi.org/10.12669/pjms.36.4.2025 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Asclepias curassavica—A Multicentric, Randomised, Double-Blind Homoeopathic Pathogenetic Trial

Homœopathic Links ◽

10.1055/s-0039-3400248 ◽

2019 ◽

Vol 32 (03) ◽

pp. 174-180

Author(s):

Rajpal Rajpal ◽

Vinay Kr. Singh ◽

V.A. Siddiqui ◽

C. Nayak ◽

A.K. Majumder ◽

...

Keyword(s):

The Body ◽

Human Beings ◽

Healthy Human ◽

Double Blind ◽

Medical Specialists ◽

Asclepias Curassavica ◽

Central Council ◽

Laboratory Investigations ◽

Two Phases ◽

Apparently Healthy

Abstract Objective To elicit the pathogenetic response of the drug Asclepias curassavica in homoeopathic potencies on healthy human beings. Methodology Drug Asclepias curassavica was proved by the Central Council for Research in Homoeopathy through randomised, double-blind, placebo-controlled method. The study was conducted at four centres. The drug was proved in two potencies (6C and 30C) on 67 apparently healthy volunteers who were selected after conducting pre-trial medical examination by the medical specialists and routine laboratory investigations. In the first phase, volunteers were given 56 doses (04 doses per day for 14 days) of placebo. In the next two phases, 56 doses (04 doses per day for 14 days) of each potency or placebo were consumed. The symptoms generated during the trial period were noted by the volunteers and elaborated by the Proving Masters. The data obtained from all the four centres was compiled at proving-cum-data processing cell at CCRH headquarters after de-coding. Observations Out of the 44 provers who were on actual drug trial, 21 manifested symptoms. Drug was able to produce symptoms in both the potencies more or less related to every part of the body. Conclusion The pathogenetic responses elicited during the proving trial expands the scope of use of the drug Asclepias curassavica and will benefit the research scholars and clinicians. These symptoms will carry more value when verified clinically.

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