Green synthesis of silver nanoparticles (Ag NPs) using Gomphrena globosa (Globe amaranth) leaf extract and their characterization

2020 ◽  
Vol 33 ◽  
pp. 2209-2216 ◽  
Author(s):  
P. Tamilarasi ◽  
P. Meena
2021 ◽  
Vol 22 (22) ◽  
pp. 12562
Author(s):  
Hanen Sellami ◽  
Shakeel Ahmad Khan ◽  
Ishaq Ahmad ◽  
Abdullah A. Alarfaj ◽  
Abdurahman H. Hirad ◽  
...  

Herein, we report the green synthesis of silver nanoparticles (OE-Ag NPs) by ecofriendly green processes using biological molecules of Olea europaea leaf extract. Green synthesized OE-Ag NPs were successfully characterized using different spectroscopic techniques. Antibacterial activity of OE-Ag NPs was assessed against four different bacteriological strains using the dilution serial method. The cytotoxic potential was determined against MCF-7 carcinoma cells using MTT assay in terms of cell viability percentage. Antioxidant properties were evaluated in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. Biocompatibility was further examined by incubating the synthesized NPs with hMSC cells for 24 h. The results were demonstrated that synthesized OE-Ag NPs presented excellent log10 reduction in the growth of all the tested bacterial strains, which as statistically equivalent (p > 0.05) to the standard antibiotic drug. Moreover, they also demonstrated excellent cytotoxic efficacy against the MCF-7 carcinoma cells compared to plant lead extract and Com-Ag NPs. Green synthesized OE-Ag NPs appeared more biocompatible to hMSC and 293T cells compared to Com-Ag NPs. Excellent biological results of the OE-Ag NPs might be attributed to the synergetic effect of NPs’ properties and the adsorbed secondary metabolites of plant leaf extract. Hence, this study suggests that synthesized OE-Ag NPs can be a potential contender for their various biological and nutraceutical applications. Moreover, this study will open a new avenue to produce biocompatible nanoparticles with additional biological functionalities from the plants.


Biomedicine ◽  
2020 ◽  
Vol 39 (4) ◽  
pp. 544-549
Author(s):  
G. K. Pratap ◽  
Manjula Shantaram

Introduction and Aim: The silver nanoparticles have attained a special place in the area of nanotechnology because of their different biological applications. Fabrication of nanoparticles using green synthesis is  done because of its wide applications in different fields such as biomedical, medicine, agriculture and food engineering. This study is to develop an easy and eco-friendly method for the synthesis of Ag-NPs using leaf extracts of the medicinal plant. Materials and Methods: The medicinal plants are rich sources of various medicinal properties. Olea dioica Roxb., leaf extract was used to investigate the effects of Ag-NPs having antibacterial activity and antioxidant capacity.  The plant leaf extract contains flavonoids, alkaloids, saponins, and phenolic compounds which acts as reducing and stabilizing agents. The green synthesized silver nanoparticles were characterized by various techniques like UV- visible spectrophotometer, FTIR spectroscopy, and SEM analysis. Results: The synthesis of sliver nanoparticles from plant source, and analysis of nano particles by UV-Vis spectra, SEM and FTIR. The biological evaluations of Ag-NPs indicated an excellent inhibitory efficacy, antioxidant and antimicrobial activity for their future applications in medicine. Conclusion: The synthesized silver nanoparticles exhibited potent antioxidant and antimicrobial activities against Gram-positive and Gram-negative bacteria. The silver (Ag-NPs) nanoparticles synthesized by the pot green synthesis method proves its potential use in various medical applications. Keywords: Silver nanoparticles; Medicinal plants; Ag-NPs; Olea dioica Roxb.,


2018 ◽  
Vol 8 (5-s) ◽  
pp. 301-305
Author(s):  
DR SHOBHA RANI TENKAYALA ◽  
Pagadala R Sougandhi ◽  
Mekala. Reddeppa ◽  
Sapara Sekhar Harini ◽  
R. Gangadhara

In this study, rapid, simple approach was applied for synthesis of silver nanoparticles by using Psidium guajava aqueous leaf extract. The plant extract acts as both reducing agent and capping agent. The green synthesized silver nanoparticles were characterized by using physic-chemical techniques viz, UV-Visible spectroscopy, Fourier Transform Infrared Spectrophotometer [FTIR], Particle size analyser and Scanning electron microscopy. UV-Visible spectrophotometer showed absorbance peak in the range of 419nm.The compounds responsible for silver ions and the functional groups present in plant extract were identified and investigated by FTIR technique. The characterization data reveals that the particles were in crystalline in nature with an average size of 62nm. The silver nanoparticles (Ag NPs) were rapidly synthesized using aqueous extract of guava leaf with AgNO3 solution within 15min at room temperature, without the involvement of any hazardous chemicals. Keywords: Nano particles, green synthesis, Silver,  Psidium guajava and reducing agents.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Arif Nazir ◽  
Saqib Farooq ◽  
Mazhar Abbas ◽  
Eman A. Alabbad ◽  
Hind Albalawi ◽  
...  

Abstract This study reports green synthesis and characterization of silver nanoparticles (Ag NPs) from Sophora mollis leaf extract. The use of S. mollis extract for preparation of Ag NPs was investigated using different techniques. Dark brown color indicates formation of nanoparticles. Fourier transform infra-red (FTIR) analysis revealed that plant extract act as a reducing and capping agent. Morphological aspects of Ag NPs were ascertained by means of SEM studies. Energy dispersive and FTIR spectroscopy results showed chemical composition and plant extract functionality respectively. X-ray diffraction (XRD) analysis showed particle size of 70 nm. Antibacterial activity of NPs was investigated by disc diffusion and minimum inhibitory concentration method. Antioxidant activity of NPs was shown by DPPH assay. The photo catalytic efficiency of synthesized Ag NPs was evaluated by degradation of methylene blue (MB) dye under UV irradiation. Ag NPs degraded MB dye up to 88% in 160 min. It is concluded that these NPs could be employed for degradation of toxic industrial effluents. Result proved the green synthesis of Ag NPs from S. mollis extract is clean, economical and safe method.


Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1007
Author(s):  
Azam Ali ◽  
Mariyam Sattar ◽  
Fiaz Hussain ◽  
Muhammad Humble Khalid Tareen ◽  
Jiri Militky ◽  
...  

The versatile one-pot green synthesis of a highly concentrated and stable colloidal dispersion of silver nanoparticles (Ag NPs) was carried out using the self-assembled tannic acid without using any other hazardous chemicals. Tannic acid (Plant-based polyphenol) was used as a reducing and stabilizing agent for silver nitrate in a mild alkaline condition. The synthesized Ag NPs were characterized for their concentration, capping, size distribution, and shape. The experimental results confirmed the successful synthesis of nearly spherical and highly concentrated (2281 ppm) Ag NPs, capped with poly-tannic acid (Ag NPs-PTA). The average particle size of Ag NPs-PTA was found to be 9.90 ± 1.60 nm. The colloidal dispersion of synthesized nanoparticles was observed to be stable for more than 15 months in the ambient environment (25 °C, 65% relative humidity). The synthesized AgNPs-PTA showed an effective antimicrobial activity against Staphylococcus Aureus (ZOI 3.0 mM) and Escherichia coli (ZOI 3.5 mM). Ag NPs-PTA also exhibited enhanced catalytic properties. It reduces 4-nitrophenol into 4-aminophenol in the presence of NaBH4 with a normalized rate constant (Knor = K/m) of 615.04 mL·s−1·mg−1. For comparison, bare Ag NPs show catalytic activity with a normalized rate constant of 139.78 mL·s−1·mg−1. Furthermore, AgNPs-PTA were stable for more than 15 months under ambient conditions. The ultra-high catalytic and good antimicrobial properties can be attributed to the fine size and good aqueous stability of Ag NPs-PTA. The unique core-shell structure and ease of synthesis render the synthesized nanoparticles superior to others, with potential for large-scale applications, especially in the field of catalysis and medical.


AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susanna Gevorgyan ◽  
Robin Schubert ◽  
Mkrtich Yeranosyan ◽  
Lilit Gabrielyan ◽  
Armen Trchounian ◽  
...  

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.


2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Akshay Rajeev Geetha ◽  
Elizabeth George ◽  
Akshay Srinivasan ◽  
Jameel Shaik

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.


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