Formulation of nanoparticle-based fluorescence dye for detection of excitable cell

2021 ◽  
Author(s):  
Jayanta Sarmah Boruah ◽  
Pankaj Kalita ◽  
Devasish Chowdhury ◽  
Manash Barthakur
Keyword(s):  
Excitable Cell ◽  
Fluorescence Dye

Deposition of Polypyrrole Onto a Novel Polymer Electrode for Use in a Biomimetic Artificial Excitable Cell Membrane

2010 ◽  
Author(s):  
Christina V. Haden ◽  
Donald A. Jordan ◽  
Pamela M. Norris
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Membrane ◽  
Surface Morphology ◽  
Polymer Membrane ◽  
Electroactive Polymer ◽  
Gating System ◽  
Excitable Cell ◽  
Semi Solid ◽  
Scanning Electron

A novel and inexpensive bucky gel electrode has been investigated for use as the electrode substrate for deposition of polypyrrole. The electroactive polymer membrane was successfully deposited and the surface morphology studied using scanning electron microscopy. Given the properties of the bucky gel electrode and its ability to conduct ions, this work establishes the first step towards a semi-solid ion-gating system to be used in further applications.

scholarly journals Non-invasive, real-time reporting drug release in vitro and in vivo

2015 ◽  
Vol 51 (32) ◽  
pp. 6948-6951 ◽  
Author(s):  
Yanfeng Zhang ◽  
Qian Yin ◽  
Jonathan Yen ◽  
Joanne Li ◽  
Hanze Ying ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Reporting System ◽  
Real Time Monitoring ◽  
Near Infrared Fluorescence ◽  
Non Invasive ◽  
Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

scholarly journals The mechanism of the NH4 ion oscillatory transport across the excitable cell membrane

2005 ◽  
pp. 5-19
Author(s):  
Cedomir Radenovic ◽  
Milos Beljanski ◽  
Georgij Maksimov ◽  
Aleksandar Kalauzi ◽  
Milan Drazic
Keyword(s):  
Membrane Potential ◽  
Cell Membrane ◽  
Transport Processes ◽  
Excitable Cell ◽  
Direct Dependence ◽  
Nh4cl Solution ◽  
Oscillatory Processes

This paper presents results on typical oscillations of the membrane potential induced by the excitation of the cell membrane by different concentrations of the NH4Cl solution. The existence of four classes of oscillations of the membrane potential and several different single and local impulses rhythmically occurring were determined. It is known that the oscillatory processes of the membrane potential are in direct dependence on oscillatory transport processes of NH4 and Cl ions across the excitable cell membrane. A hypothesis on a possible mechanism of oscillatory transport processes of NH4 and Cl ions across the excitable cell membrane is also presented.

scholarly journals Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue

2021 ◽  
Vol 22 (21) ◽  
pp. 11467
Author(s):  
Joshua Kleine ◽  
Sandra Leisz ◽  
Chalid Ghadban ◽  
Tim Hohmann ◽  
Julian Prell ◽  
...  
Keyword(s):  
Hippocampal Slice ◽  
Cellular Damage ◽  
Regenerated Cellulose ◽  
Clinical Settings ◽  
Oxidized Regenerated Cellulose ◽  
Tissue Properties ◽  
Neuronal Tissue ◽  
Fluorescence Dye ◽  
Isolectin B4 ◽  
Hematoxylin Eosin

Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery. The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue. Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells. Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation. Whereas Equicel® led to a neutral pH, Tabotamp® (pH 2.8) and Equitamp® (pH 4.8) caused a significant reduction of pH (p < 0.001). Equicel® and Tabotamp® increased cytotoxicity significantly in several cell lines (p < 0.01). On OHSC, Tabotamp® and Equicel® led to a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01). Equicel® increased strongly the number of microglia cells after 24 h (p < 0.001). Microglia cells were not detectable after Tabotamp® treatment, presumably due to an artifact caused by strong pH reduction. In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.

Sperm activation in species with external fertilisation

Zygote ◽  
1994 ◽  
Vol 2 (4) ◽  
pp. 359-361 ◽  
Author(s):  
Elisabetta Tosit
Keyword(s):  
Sea Urchin ◽  
Acrosome Reaction ◽  
Quiescent State ◽  
Sperm Activation ◽  
Excitable Cell

The spermatoozoon is an excitable cell that responds to specific effectors by rapidly changing its behaviour. In species with external fertilisation, spermatozoa are stored in a quiescent state in the testis, but within seconds after spawning, dilution into water triggers several activation events such as increases in motility and respiration. In some species, these are followed by the acrosome reaction, an exocytotic process that allows the spermatozoon to penetrate the egg investments and activate the egg (Dale, 1983). The majority of information on sperm activation has come from the sea urchin; secondarily teleosts and starfish have proved to be useful models.

Dynamic oxygen mapping in the root zone by fluorescence dye imaging combined with neutron radiography

2011 ◽  
Vol 12 (1) ◽  
pp. 63-74 ◽  
Author(s):  
Nicole Rudolph ◽  
Hanna G. Esser ◽  
Andrea Carminati ◽  
Ahmad B. Moradi ◽  
André Hilger ◽  
...  
Keyword(s):  
Root Zone ◽  
Neutron Radiography ◽  
Fluorescence Dye

scholarly journals Fluorescence-Labeled Amyloid Beta Monomer: A Molecular Dynamical Study

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3524
Author(s):  
János Gera ◽  
Gábor Paragi
Keyword(s):  
Single Molecule ◽  
Early Stage ◽  
Conformational Space ◽  
Dynamic Simulations ◽  
Dynamical Study ◽  
Fluorescence Dye ◽  
Aβ Aggregation ◽  
The Difference ◽  
Open Question ◽  
The Impact

The aggregation process of the Amyloidβ (Aβ) peptide is one of the central questions in Alzheimers’s research. Fluorescence-labeled single-molecule detection is a novel technique concerning the early stage investigation of Aβ aggregation, where the labeling dyes are covalently bound to the Aβ monomer. As the influence of the dye on the conformational space of the Aβ monomer can be significant, its effect on the seeding process is an open question. The applied fluorescent molecule continuously switches between an active (ON) and an inactive (OFF) state, where the latter supports an extra rotational restriction at many commercially available dyes. However, only a few theoretical studies simulated the Aβ monomer in the presence of a dye and none of them considered the difference between the ON and the OFF states. Therefore, we examined the impact of a selected fluorescence dye (Alexa 568) on the conformational space of the monomeric Aβ(1–42) peptide in its ON and OFF state by replica exchange molecular dynamic simulations. Investigations on secondary structure elements as well as dye-peptide contact analysis for the monomers are presented. Experimental and theoretical NMR shifts were contrasted to qualify the calculation protocol and theoretical values of the labeled and the non-labeled peptide were also compared. We found that the first five residues have higher helical propensity in the presence of the dye, and electrostatic properties could strongly affect the connection between the dye and the peptide parts.

scholarly journals A high-sensitive and non-radioisotopic fluorescence dye method for evaluating bacterial adhesion to denture materials

2013 ◽  
Vol 32 (4) ◽  
pp. 585-591 ◽  
Author(s):  
Yoko SAKUMA ◽  
Jumpei WASHIO ◽  
Keiichi SASAKI ◽  
Nobuhiro TAKAHASHI
Keyword(s):  
Bacterial Adhesion ◽  
Fluorescence Dye
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