A hybrid roll-to-roll AFM set-up for high throughput tip-based nano-machining

2015 ◽  
Vol 6 ◽  
pp. 10-13 ◽  
Author(s):  
Emmanuel Brousseau ◽  
Raheem S.J. Al-Musawi ◽  
Damien Lebiez
Keyword(s):  
High Throughput ◽  
Roll To Roll ◽  
Set Up

scholarly journals High Throughput Roll-to-Roll Production of Microfluidic Chips

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 1054
Author(s):  
Martin Smolka ◽  
Stephan Ruttloff ◽  
Dieter Nees ◽  
Christine Prietl ◽  
Valentin Satzinger ◽  
...  
Keyword(s):  
High Throughput ◽  
Inorganic Ions ◽  
Soil Nutrient ◽  
Microfluidic Chips ◽  
Large Area ◽  
Nutrient Analysis ◽  
Roll To Roll ◽  
Large Numbers ◽  
Set Up

A high throughput manufacturing process of microfluidic chips based on Roll-to-Roll imprinting is presented. With this procedure, microfluidic patterns can be produced on large area polymer substrates. The subsequent steps of inlet drilling, bonding and electrode printing are set-up on large area processes, too. Overall, this strategy allows highly parallelized processing of large numbers of chips—all costly steps of individual chip handling are avoided. The chips were used for the characterization of inorganic ions for soil nutrient analysis.

scholarly journals Automatic 1D 1H NMR Metabolite Quantification for Bioreactor Monitoring

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 157
Author(s):  
Roy Chih Chung Wang ◽  
David A. Campbell ◽  
James R. Green ◽  
Miroslava Čuperlović-Culf
Keyword(s):  
High Throughput ◽  
Nmr Metabolomics ◽  
Gene Therapies ◽  
Quantification Method ◽  
Minimal Sample ◽  
Enhanced Production ◽  
Bioreactor Monitoring ◽  
Online Measurements ◽  
Metabolite Quantification ◽  
Set Up

High-throughput metabolomics can be used to optimize cell growth for enhanced production or for monitoring cell health in bioreactors. It has applications in cell and gene therapies, vaccines, biologics, and bioprocessing. NMR metabolomics is a method that allows for fast and reliable experimentation, requires only minimal sample preparation, and can be set up to take online measurements of cell media for bioreactor monitoring. This type of application requires a fully automated metabolite quantification method that can be linked with high-throughput measurements. In this review, we discuss the quantifier requirements in this type of application, the existing methods for NMR metabolomics quantification, and the performance of three existing quantifiers in the context of NMR metabolomics for bioreactor monitoring.

scholarly journals Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Marie-Christine Carpentier ◽  
Cécile Bousquet-Antonelli ◽  
Rémy Merret
Keyword(s):  
Gene Expression ◽  
Quality Control ◽  
Transcriptional Regulation ◽  
High Throughput ◽  
Library Preparation ◽  
Working Day ◽  
Set Up ◽  
Post Transcriptional Regulation ◽  
Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

Modular automated bottom-up proteomic sample preparation for high-throughput applications v1

2021 ◽  
Author(s):  
Yan Chen ◽  
Nurgul Kaplan Lease ◽  
Jennifer Gin ◽  
Tad Ogorzalek ◽  
Paul D. Adams ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Tryptic Digestion ◽  
Gram Negative Bacteria ◽  
Bottom Up ◽  
Preparation Methods ◽  
Gram Negative ◽  
E Coli ◽  
Protocol Development ◽  
Set Up

Manual proteomic sample preparation methods limit sample throughput and often lead to poor data quality when thousands of samples must be analyzed. Automated workflows are increasingly used to overcome these issues for some (or even all) of the sample preparation steps. Here, we detail three optimised step-by-step protocols to: (A) lyse Gram-negative bacteria and fungal cells; (B) quantify the amount of protein extracted; and (C) normalize the amount of protein and set up tryptic digestion. These protocols have been developed to facilitate rapid, low variance sample preparation of hundreds of samples, be easily implemented on widely-available Beckman-Coulter Biomek automated liquid handlers, and allow flexibility for future protocol development. By using this workflow 50 micrograms of peptides for 96 samples can be prepared for tryptic digestion in under an hour. We validate these protocols by analyzing 47 E. coli and R. toruloides samples and show that this modular workflow provides robust, reproducible proteomic samples for high-throughput applications. The expected results from these protocols are 94 peptide samples from Gram-negative bacterial and fungal cells prepared for bottom-up quantitative proteomic analysis without the need for desalting column cleanup and with peptide variance (CVs) below 15%.

scholarly journals Toward High‐Throughput Texturing of Polymer Foils for Enhanced Light Trapping in Flexible Perovskite Solar Cells Using Roll‐to‐Roll Hot Embossing

2020 ◽  
Vol 22 (4) ◽  
pp. 1901217 ◽  
Author(s):  
Marcos Soldera ◽  
Qiong Wang ◽  
Flavio Soldera ◽  
Valentin Lang ◽  
Antonio Abate ◽  
...  
Keyword(s):  
Solar Cells ◽  
High Throughput ◽  
Light Trapping ◽  
Perovskite Solar Cells ◽  
Hot Embossing ◽  
Roll To Roll ◽  
Polymer Foils

scholarly journals HIGH-THROUGHPUT ROLL-TO-ROLL PRODUCTION OF BIO-FUNCTIONALIZED POLYMER COMPONENTS

2020 ◽  
Vol 5 (1) ◽  
pp. 11-16
Author(s):  
Pelin TOREN ◽  
◽  
Markus RUMPLER ◽  
Martin SMOLKA ◽  
Anja HAASE ◽  
...  
Keyword(s):  
High Throughput ◽  
Roll To Roll

scholarly journals High-throughput roll-to-roll production of polymer biochips for multiplexed DNA detection in point-of-care diagnostics

Lab on a Chip ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 4106-4117
Author(s):  
Pelin Toren ◽  
Martin Smolka ◽  
Anja Haase ◽  
Ursula Palfinger ◽  
Dieter Nees ◽  
...  
Keyword(s):  
High Throughput ◽  
Nanoimprint Lithography ◽  
Dna Detection ◽  
Point Of Care ◽  
Roll To Roll ◽  
Flexible Polymer ◽  
Nano Structures ◽  
Point Of Care Diagnostics ◽  
Polymer Foils

Roll-to-roll UV nanoimprint lithography has superior advantages for high-throughput manufacturing of micro- or nano-structures on flexible polymer foils with various geometries and configurations.

OLED Manufacturing on Flexible Substrates Towards Roll-to-Roll

MRS Advances ◽  
2019 ◽  
Vol 4 (24) ◽  
pp. 1367-1375 ◽  
Author(s):  
Dongxiang Wang ◽  
Jacqueline Hauptmann ◽  
Christian May
Keyword(s):  
High Throughput ◽  
Present Status ◽  
Manufacturing Cost ◽  
Flexible Substrates ◽  
Large Area ◽  
Thin Glass ◽  
Production Capability ◽  
Roll To Roll ◽  
Pet Film

ABSTRACTLarge area lighting OLEDs manufactured in a Roll-to-Roll (R2R) fashion enable the well-longed production capability with considerably high throughput based on flexible substrates, hence largely reduced OLED manufacturing cost. This paper will outline the present status of R2R OLED fabrication on ultra-thin glass with the focus on transparent OLED devices and how to perform segmentation by printing of silver- and dielectric pastes. Ultra-thin glass (UTG) is laminated on a PET film to avoid fabrication interruptions when glass cracks occur during the Roll-to-Roll process. The R2R fabricated flexible OLEDs also show key-values comparable to conventional OLEDs fabricated on small rigid glass in lab-scale.

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