Set-up for combinatorial electrochemical synthesis and high-throughput investigation of organic semiconductor polymers for electronic devices

High-throughput metabolomics can be used to optimize cell growth for enhanced production or for monitoring cell health in bioreactors. It has applications in cell and gene therapies, vaccines, biologics, and bioprocessing. NMR metabolomics is a method that allows for fast and reliable experimentation, requires only minimal sample preparation, and can be set up to take online measurements of cell media for bioreactor monitoring. This type of application requires a fully automated metabolite quantification method that can be linked with high-throughput measurements. In this review, we discuss the quantifier requirements in this type of application, the existing methods for NMR metabolomics quantification, and the performance of three existing quantifiers in the context of NMR metabolomics for bioreactor monitoring.

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Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽

10.3390/plants10030466 ◽

2021 ◽

Vol 10 (3) ◽

pp. 466

Author(s):

Marie-Christine Carpentier ◽

Cécile Bousquet-Antonelli ◽

Rémy Merret

Keyword(s):

Gene Expression ◽

Quality Control ◽

Transcriptional Regulation ◽

High Throughput ◽

Library Preparation ◽

Working Day ◽

Set Up ◽

Post Transcriptional Regulation ◽

Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

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Modular automated bottom-up proteomic sample preparation for high-throughput applications v1

10.17504/protocols.io.bwxypfpw ◽

2021 ◽

Author(s):

Yan Chen ◽

Nurgul Kaplan Lease ◽

Jennifer Gin ◽

Tad Ogorzalek ◽

Paul D. Adams ◽

...

Keyword(s):

Sample Preparation ◽

High Throughput ◽

Tryptic Digestion ◽

Gram Negative Bacteria ◽

Bottom Up ◽

Preparation Methods ◽

Gram Negative ◽

E Coli ◽

Protocol Development ◽

Set Up

Manual proteomic sample preparation methods limit sample throughput and often lead to poor data quality when thousands of samples must be analyzed. Automated workflows are increasingly used to overcome these issues for some (or even all) of the sample preparation steps. Here, we detail three optimised step-by-step protocols to: (A) lyse Gram-negative bacteria and fungal cells; (B) quantify the amount of protein extracted; and (C) normalize the amount of protein and set up tryptic digestion. These protocols have been developed to facilitate rapid, low variance sample preparation of hundreds of samples, be easily implemented on widely-available Beckman-Coulter Biomek automated liquid handlers, and allow flexibility for future protocol development. By using this workflow 50 micrograms of peptides for 96 samples can be prepared for tryptic digestion in under an hour. We validate these protocols by analyzing 47 E. coli and R. toruloides samples and show that this modular workflow provides robust, reproducible proteomic samples for high-throughput applications. The expected results from these protocols are 94 peptide samples from Gram-negative bacterial and fungal cells prepared for bottom-up quantitative proteomic analysis without the need for desalting column cleanup and with peptide variance (CVs) below 15%.

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Analysis of low-frequency noise characterisation set-up for electronic devices

2018 Australian Microwave Symposium (AMS) ◽

10.1109/ausms.2018.8346971 ◽

2018 ◽

Author(s):

Jason T. Hodges ◽

Michael Heimlich ◽

Sourabh Khandelwal

Keyword(s):

Electronic Devices ◽

Low Frequency ◽

Frequency Noise ◽

Low Frequency Noise ◽

Set Up

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High-Throughput Crystallization Pipeline at the Crystallography Core Facility of the Institut Pasteur

Molecules ◽

10.3390/molecules24244451 ◽

2019 ◽

Vol 24 (24) ◽

pp. 4451 ◽

Cited By ~ 6

Author(s):

Patrick Weber ◽

Cédric Pissis ◽

Rafael Navaza ◽

Ariel E. Mechaly ◽

Frederick Saul ◽

...

Keyword(s):

High Throughput ◽

Sequence Data ◽

Data Bank ◽

Whole Genome Sequence ◽

Whole Genome ◽

X Ray Diffraction ◽

Sequencing Technology ◽

X Ray ◽

Set Up ◽

General User

The availability of whole-genome sequence data, made possible by significant advances in DNA sequencing technology, led to the emergence of structural genomics projects in the late 1990s. These projects not only significantly increased the number of 3D structures deposited in the Protein Data Bank in the last two decades, but also influenced present crystallographic strategies by introducing automation and high-throughput approaches in the structure-determination pipeline. Today, dedicated crystallization facilities, many of which are open to the general user community, routinely set up and track thousands of crystallization screening trials per day. Here, we review the current methods for high-throughput crystallization and procedures to obtain crystals suitable for X-ray diffraction studies, and we describe the crystallization pipeline implemented in the medium-scale crystallography platform at the Institut Pasteur (Paris) as an example.

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Non-covalent interaction controlled 2D organic semiconductor films: Molecular self-assembly, electronic and optical properties, and electronic devices

Surface Science Reports ◽

10.1016/j.surfrep.2020.100481 ◽

2020 ◽

Vol 75 (2) ◽

pp. 100481 ◽

Cited By ~ 3

Author(s):

Jia Lin Zhang ◽

Xin Ye ◽

Chengding Gu ◽

Cheng Han ◽

Shuo Sun ◽

...

Keyword(s):

Optical Properties ◽

Organic Semiconductor ◽

Self Assembly ◽

Electronic Devices ◽

Semiconductor Films ◽

Electronic And Optical Properties ◽

Covalent Interaction

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A biotechnological approach for the development of new antifungal compounds to protect the environment and the human health

Journal of Public Health Research ◽

10.4081/jphr.2015.613 ◽

2015 ◽

Vol 4 (3) ◽

Cited By ~ 3

Author(s):

Claudia Zani ◽

Francesco Maria Restivo ◽

Mauro Carcelli ◽

Donatella Feretti ◽

Giorgio Pelosi ◽

...

Keyword(s):

Human Health ◽

High Throughput ◽

High Throughput Screening ◽

Animal Feed ◽

Po Valley ◽

Biotechnological Approach ◽

Relevant Role ◽

Food Economy ◽

Set Up ◽

New Compounds

Background. In the Po Valley aflatoxins play a relevant role: the local food economy is heavily based on cereal cultivations for animal feed and human nutrition. Aims of this project are the identification of new compounds that inhibit Aspergillus proliferation, the development of new inhibitors of aflatoxins production, and the set-up a practical screening procedure to identify the most effective and safe compounds. Design and Methods. New compounds will be synthetized with natural origin molecules as ligands and endogenous metal ions to increase their bioavailability for the fungi as metal complexes. A biotechnological high-throughput screening will be set up to identify efficiently the most powerful substances. The newly synthesized compounds with effective antifungal activities, will be evaluated with battery of tests with different end-points to assess the toxic potential risk for environmental and human health. Expected impact of the study for public health. The fundamental step in the project will be the synthesis of new compounds and the study of their capability to inhibit aflatoxin biosynthesis. A new, simple, inexpensive and high-throughput method to screen the anti-fungine and anti-mycotoxin activity of the new synthesised compounds will be applied. The evaluation of possible risks for humans due to toxic and genotoxic activities of the molecules will be made with a new approach using different types of cells (bacteria, plants and human cells).

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Assay Concordance between SPA and TR-FRET in High-Throughput Screening

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057106288183 ◽

2006 ◽

Vol 11 (6) ◽

pp. 606-616 ◽

Cited By ~ 25

Author(s):

Oliver Von Ahsen ◽

Anne Schmidt ◽

Monika Klotz ◽

Karsten Parczyk

Keyword(s):

High Throughput ◽

High Throughput Screening ◽

Statistical Significance ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Time Resolved ◽

Detection Technology ◽

Significant Difference ◽

Set Up ◽

Detection Technologies

High-throughput screening (HTS) of large chemical libraries has become the main source of new lead compounds for drug development. Several specialized detection technologies have been developed to facilitate the cost- and time-efficient screening of millions of compounds. However, concerns have been raised, claiming that different HTS technologies may produce different hits, thus limiting trust in the reliability of HTS data. This study was aimed to investigate the reliability of the authors most frequently used assay techniques: scintillation proximity assay (SPA) and homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET). To investigate the data concordance between these 2 detection technologies, the authors screened a large subset of the Schering compound library consisting of 300,000 compounds for inhibitors of a nonreceptor tyrosine kinase. They chose to set up this study in realistic HTS scale to ensure statistical significance of the results. The findings clearly demonstrate that the choice of detection technology has no significant impact on hit finding, provided that assays are biochemically equivalent. Data concordance is up to 90%. The little differences in hit findings are caused by threshold setting but not by systematic differences between the technologies. The most significant difference between the compared techniques is that in the SPA format, more false-positive primary hits were obtained.

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