Control of Fusarium wilt of lisianthus by reassembling the microbial community in infested soil through reductive soil disinfestation

The influence of crop rotation and wheather conditions on the Fusarium population in soil and on Fusarium wilt of flax was examined in the Institute of Native Natural Fibres in 1968-1975. The experiments were carried out in various regions of flax planting in Poland during 8 years. Crop rotation had a significant influence on the process of biological soil disinfestation. Fusarium population in soil increased after planting flax, wheat, barley and sugar-beet, and decreased or did not change after rape, hemp, potato and leguminous plants. The crop rotation significantly influenced the composition of the Fusarium population as far as species are concerned. The highest wilt infection of flax was found in plantings after flax, sugarbeet and wheat and the weakest in plantings after rape. A six-year crop rotation was sufficient to eliminate the wilt pathogen from infested soil. But on flax-sick soil the process of biological disinfestation proceeded more slowly and a seven-year interval between flax crops was too short.

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Soil disinfestation with dimethyl disulfide for management of Fusarium wilt on lettuce in Italy

Journal of Plant Diseases and Protection ◽

10.1007/s41348-017-0071-2 ◽

2017 ◽

Vol 124 (4) ◽

pp. 361-370 ◽

Cited By ~ 12

Author(s):

G. Gilardi ◽

M. L. Gullino ◽

A. Garibaldi

Keyword(s):

Fusarium Wilt ◽

Dimethyl Disulfide ◽

Soil Disinfestation

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Analysis of Environmental Variables and Carbon Input on Soil Microbiome, Metabolome and Disease Control Efficacy in Strawberry Attributable to Anaerobic Soil Disinfestation

Microorganisms ◽

10.3390/microorganisms9081638 ◽

2021 ◽

Vol 9 (8) ◽

pp. 1638

Author(s):

Shashika S. Hewavitharana ◽

Emmi Klarer ◽

Joji Muramoto ◽

Carol Shennan ◽

Mark Mazzola

Keyword(s):

Disease Control ◽

Fusarium Wilt ◽

Environmental Variables ◽

Incubation Temperature ◽

Soil Microbiome ◽

Charcoal Rot ◽

Soil Disinfestation ◽

Anaerobic Soil Disinfestation ◽

Input Type ◽

Incubation Duration

Charcoal rot and Fusarium wilt, caused by Macrophomina phaseolina and Fusarium oxysporum f. sp. fragariae, respectively, are major soil-borne diseases of strawberry that have caused significant crop losses in California. Anaerobic soil disinfestation has been studied as an industry-level option to replace soil fumigants to manage these serious diseases. Studies were conducted to discern whether Gramineae carbon input type, incubation temperature, or incubation duration influences the efficacy of this disease control tactic. In experiments conducted using ‘low rate’ amendment applications at moderate day/night temperatures (24/18 °C), and carbon inputs (orchard grass, wheat, and rice bran) induced an initial proliferation and subsequent decline in soil density of the Fusarium wilt pathogen. This trend coincided with the onset of anaerobic conditions and a corresponding generation of various anti-fungal compounds, including volatile organic acids, hydrocarbons, and sulfur compounds. Generation of these metabolites was associated with increases in populations of Clostridium spp. Overall, carbon input and incubation temperature, but not incubation duration, significantly influenced disease suppression. All Gramineae carbon inputs altered the soil microbiome and metabolome in a similar fashion, though the timing and maximum yield of specific metabolites varied with input type. Fusarium wilt and charcoal rot suppression were superior when anaerobic soil disinfestation was conducted using standard amendment rates of 20 t ha−1 at elevated temperatures combined with a 3-week incubation period. Findings indicate that anaerobic soil disinfestation can be further optimized by modulating carbon source and incubation temperature, allowing the maximum generation of antifungal toxic volatile compounds. Outcomes also indicate that carbon input and environmental variables may influence treatment efficacy in a target pathogen-dependent manner which will require pathogen-specific optimization of treatment protocols.

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Soil microbial community structure in cucumber rhizosphere of different resistance cultivars to fusarium wilt

FEMS Microbiology Ecology ◽

10.1111/j.1574-6941.2010.00859.x ◽

2010 ◽

Vol 72 (3) ◽

pp. 456-463 ◽

Cited By ~ 44

Author(s):

Huaiying Yao ◽

Fengzhi Wu

Keyword(s):

Community Structure ◽

Microbial Community ◽

Fusarium Wilt ◽

Microbial Community Structure ◽

Soil Microbial Community ◽

Soil Microbial Community Structure ◽

Soil Microbial

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Histopathology comparison and phenylalanine ammonia lyase (PAL) gene expressions in Fusarium wilt infected watermelons

Australian Journal of Agricultural Research ◽

10.1071/ar08066 ◽

2008 ◽

Vol 59 (12) ◽

pp. 1146 ◽

Cited By ~ 17

Author(s):

P.-F. L. Chang ◽

C.-C. Hsu ◽

Y.-H. Lin ◽

K.-S. Chen ◽

J.-W. Huang ◽

...

Keyword(s):

Fusarium Wilt ◽

Phenylalanine Ammonia Lyase ◽

Citrullus Lanatus ◽

Spontaneous Mutation ◽

Limiting Factors ◽

Lower Percentage ◽

Post Inoculation ◽

Infested Soil ◽

Gene Expressions ◽

Shoot Base

Fusarium wilt disease of watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai), caused by Fusarium oxysporum f. sp. niveum (FON), is one of the limiting factors of worldwide watermelon production. In this study, a Fusarium wilt resistant watermelon JSB, which was derived from a spontaneous mutation of the susceptible Sugar Baby (SB), was used to investigate histopathology. The number and diameter of xylem vessels in the root (10 mm below the shoot base) of resistant JSB plants were significantly higher than those in susceptible SB plants. At 9 days post inoculation (dpi), using the plate assay on Nash-PCNB media, FON could be recovered from 86% of the roots in the symptomless plants of both watermelon lines, and from 55% and 64% of the stem segments (5 mm above the shoot base) in resistant and susceptible plants, respectively. In paraffin and free-hand tissue sections, at 8, 13, and 35 dpi, the xylem of roots and stems close to the soil surface in resistant watermelon JSB plants was also colonised by FON, but to a much lower percentage than the susceptible SB ones. No colonisation below the middle of stems was observed in the resistant JSB plants. The susceptible plants grown in infested soil were all dead by 35 dpi, while the resistant plants remained healthy. These observations suggest that reducing FON colonisation in the vascular systems of the host may contribute to the resistance in JSB. Furthermore, the higher expression of the phenylalanine ammonia lyase (PAL) gene in JSB induced by FON and the effects of PAL inhibitor on the resistance of JSB suggested that PAL is involved in resistance of watermelon to Fusarium wilt pathogen.

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Bacillus amyloliquefaciens B1408 suppresses Fusarium wilt in cucumber by regulating the rhizosphere microbial community

Applied Soil Ecology ◽

10.1016/j.apsoil.2018.12.011 ◽

2019 ◽

Vol 136 ◽

pp. 55-66 ◽

Cited By ~ 15

Author(s):

Lingjuan Han ◽

Zeyu Wang ◽

Na Li ◽

Yonghong Wang ◽

Juntao Feng ◽

...

Keyword(s):

Microbial Community ◽

Fusarium Wilt ◽

Bacillus Amyloliquefaciens ◽

Rhizosphere Microbial Community

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In Planta and Soil Quantification of Fusarium oxysporum f. sp. ciceris and Evaluation of Fusarium Wilt Resistance in Chickpea with a Newly Developed Quantitative Polymerase Chain Reaction Assay

Phytopathology ◽

10.1094/phyto-07-10-0190 ◽

2011 ◽

Vol 101 (2) ◽

pp. 250-262 ◽

Cited By ~ 35

Author(s):

Daniel Jiménez-Fernández ◽

Miguel Montes-Borrego ◽

Rafael M. Jiménez-Díaz ◽

Juan A. Navas-Cortés ◽

Blanca B. Landa

Keyword(s):

Polymerase Chain Reaction ◽

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Plant Root ◽

Quantitative Polymerase Chain Reaction ◽

Infested Soil ◽

In Planta ◽

Field Plot ◽

Chain Reaction ◽

Polymerase Chain

Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceris can be managed by risk assessment and use of resistant cultivars. A reliable method for the detection and quantification of F. oxysporum f. sp. ciceris in soil and chickpea tissues would contribute much to implementation of those disease management strategies. In this study, we developed a real-time quantitative polymerase chain reaction (q-PCR) protocol that allows quantifying F. oxysporum f. sp. ciceris DNA down to 1 pg in soil, as well as in the plant root and stem. Use of the q-PCR protocol allowed quantifying as low as 45 colony forming units of F. oxysporum f. sp. ciceris per gram of dry soil from a field plot infested with several races of the pathogen. Moreover, the q-PCR protocol clearly differentiated susceptible from resistant chickpea reactions to the pathogen at 15 days after sowing in artificially infested soil, as well as the degree of virulence between two F. oxysporum f. sp. ciceris races. Also, the protocol detected early asymptomatic root infections and distinguished significant differences in the level of resistance of 12 chickpea cultivars that grew in that same field plot infested with several races of the pathogen. Use of this protocol for fast, reliable, and cost-effective quantification of F. oxysporum f. sp. ciceris in asymptomatic chickpea tissues at early stages of the infection process can be of great value for chickpea breeders and for epidemiological studies in growth chambers, greenhouses and field-scale plots.

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Suppression of Fusarium Wilt Caused by Fusarium oxysporum f. sp. niveum Race 2 on Grafted Triploid Watermelon

Plant Disease ◽

10.1094/pdis-01-14-0005-re ◽

2014 ◽

Vol 98 (10) ◽

pp. 1326-1332 ◽

Cited By ~ 13

Author(s):

Anthony P. Keinath ◽

Richard L. Hassell

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Disease Incidence ◽

The United States ◽

Infested Soil ◽

Marketable Yield ◽

Race 1 ◽

Susceptible Control ◽

Triploid Watermelon ◽

Race 2

Fusarium wilt of watermelon, caused by the soilborne fungal pathogen Fusarium oxysporum f. sp. niveum race 2, is a serious, widespread disease present in major watermelon-growing regions of the United States and other countries. ‘Fascination,’ a high yielding triploid resistant to race 1, is grown in southeastern states in fields that contain a mixture of races 1 and 2. There is some benefit to using cultivars with race 1 resistance in such fields, even though Fascination is susceptible to Fusarium wilt caused by race 2. Experiments in 2012 and 2013 were done in fields infested primarily with race 2 and a mixture of races 1 and 2, respectively. Fascination was grafted onto four rootstock cultivars: bottle gourd (Lagenaria siceraria) ‘Macis’ and ‘Emphasis’ and interspecific hybrid squash (Cucurbita maxima× C. moschata) ‘Strong Tosa’ and ‘Carnivor.’ Nongrafted and self-grafted Fascination were used as susceptible control treatments. In both experiments, mean incidence of plants with symptoms of Fusarium wilt was ≥52% in the susceptible control treatments and ≤6% on the grafted rootstocks. Disease incidence did not differ between rootstock species or cultivars. In both years, Fascination grafted onto Strong Tosa and Macis produced more marketable-sized fruit than the susceptible control treatments. Grafted Emphasis and Carnivor also produced more fruit than the control treatments in 2012. The cucurbit rootstocks suppressed Fusarium wilt caused by race 2 and increased marketable yield of triploid watermelon grown in infested soil.

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Soil Microbial Functional Diversity from Different Infection Grades of Banana Fusarium Wilt (Fusarium oxysporum f. sp. Cubense)

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.295-298.2274 ◽

2013 ◽

Vol 295-298 ◽

pp. 2274-2280 ◽

Cited By ~ 2

Author(s):

Xiao Deng ◽

Qin Fen Li ◽

Xian Wen Hou ◽

Chun Yuan Wu

Keyword(s):

Microbial Community ◽

Fusarium Oxysporum ◽

Functional Diversity ◽

Fusarium Wilt ◽

Soil Microbial Community ◽

Rhizosphere Soil ◽

Principal Component ◽

Soil Microbial ◽

Metabolic Characteristics ◽

Environmental Measures

Thirty rhizosphere and non-rhizosphere soil samples from different infection grades(0, I, III, V and VII) of three typical banana plots(Jianfeng, Shiyuetian, Chongpo) infected by banana fusarium wilt (Fusarium oxysporum f. sp. cubense) in Hainan province were collected to study the microbial community functional diversity applying Biolog-ECO microplates technology. The results are as follows: (1) Overall carbon source metabolic capacities of soil microbial community weaken with increasing of infection grades of banana fusarium wilt. (2) Richness indices, Simpson indices, Shannon indices and McIntosh indices of soil microbial community gradually decreased with increasing of infection grades of banana fusarium wilt. (3) Principal component analysis show that metabolic characteristics of soil microbial community significantly change between the healthy plants and diseased plants in the same banana plot. The results would provide information for explaining the pathogenesis of banana fusarium wilt and controlling its incidence by applying microbial ecology to regulate soil environmental measures.

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