Effect of ammonium acetate on alcohol fermentation in cassava-alcohol fermentation process

2021 ◽  
pp. 126868
Author(s):  
Xinchao Yang ◽  
Jiadong Huang ◽  
Yuling Yang ◽  
Chunjiang Ye ◽  
Yuanxiu Wang ◽  
...  
Chirality ◽  
2011 ◽  
Vol 23 (5) ◽  
pp. 424-428 ◽  
Author(s):  
Yuele Lu ◽  
Jinling Diao ◽  
Xu Gu ◽  
Yanfeng Zhang ◽  
Peng Xu ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5737
Author(s):  
Josef Licek ◽  
Mojmir Baron ◽  
Jiri Sochor

This study deals with the effects of the use of a mixture of medium-chain fatty acids (MCFA) at the end of the alcohol fermentation process on the content of carbonyl compounds in wine. During the experiment, the effects of the addition of MCFA at doses of 10 and 20 mg/L were compared to the termination of alcohol fermentation using cross-flow filtration and chilling treatments. Individual carbonyl compounds were determined by HPLC analysis. The experiment showed that the addition of MCFA caused a reduction of the acetaldehyde content compared to the chilling process, and a reduction of the diacetyl content compared to cross-flow filtration. Throughout the experiment, a lower level of total carbonyl compounds was observed after the addition of MCFA.


2021 ◽  
Vol 5 (1) ◽  
pp. 73
Author(s):  
Desi Riana Saputri ◽  
Fenzy Putri Liewenti ◽  
Stanislaus Dimas Indra

Cocoa beans from Indonesian farmers are not optimal to utilize dan has low quality because they have not done fermentation processing. The aim of this study was to process cocoa beans through fermentation by using Aspergillus niger and Saccharomyces cerevisiae by developing cocoa-pulp byproduct that can be converted to be alcohol. Fermentation process had done in two type of containers such as plastic and stainless steel material. It was observed to know the presence of olygodinamic effect from stainless steel material. The results showed that cacao pulp can be fermented by using Aspergillus niger and Saccharomyces cerevisiae in sensory test method with hyphae growth and strong aromatic from alcohol observation. Stainless steel material in fermentor tub did not give the oligodynamic effect for Aspergillus niger fungus and Saccharomyces cerevisiae growth. The Fermentation process produced 0.66 mL of alcohol from one kilogram fresh cocoa beans. The maximum bioethanol product is that happened in 4th day fermentation process about 5.30 %.


Author(s):  
A.-M. Ladhoff ◽  
B.J. Thiele ◽  
Ch. Coutelle ◽  
S. Rosenthal

The suggested precursor-product relationship between the nuclear pre-mRNA and the cytoplasmic mRNA has created increased interest also in the structure of these RNA species. Previously we have been published electron micrographs of individual pre-mRNA molecules from erythroid cells. An intersting observation was the appearance of a contour, probably corresponding to higher ordered structures, on one end of 10 % of the pre-mRNA molecules from erythroid rabbit bone marrow cells (Fig. 1A). A virtual similar contour was observed in molecules of 9S globin mRNA from rabbit reticulocytes (Fig. 1B). A structural transformation in a linear contour occurs if the RNA is heated for 10 min to 90°C in the presence of 80 % formamide. This structural transformation is reversible when the denatured RNA is precipitated and redissolved in 0.2 M ammonium acetate.


Author(s):  
N. H. Sarkar ◽  
Dan H. Moore

Mouse mammary tumor virus (MTV) is believed to contain about 0.8% single stranded ribonucleic acid (RNA). This value of RNA content was estimated on a dry weight basis. The subject of this report is an attempt to visualize the RNA molecules of MTV particles.MTV particles were isolated from RIII mouse (tumor incidence approximately 80%) milk according to the method described by Lyons and Moore. Purified virions from 5 ml of milk were finally suspended in 0.2 ml of PBS, pH 7.4 and was mixed with an equal volume of pronase (5 mg/ml). This mixture was incubated at 37°C for an hour. RNA was extracted three times using freshly prepared cold phenol. It was then treated three times with cold ethyl ether to remove any trace of phenol. The RNA thus extracted was divided into two parts. One part was diluted four fold with 8M urea to avoid aggregation of the molecules. The other part was left untreated. Both samples were then mixed with an equal volume of 1M ammonium acetate, adjusted to pH 8.0 with NH3 containing chymotrypsin at a concentration of 0.01%.


Author(s):  
E.T. O’Toole ◽  
G.P. Wray ◽  
J.R. Kremer ◽  
J.R. Mcintosh

Ultrarapid freezing and cryomicroscopy of frozen hydrated material makes it possible to visualize samples that have never been exposed to chemical fixatives, dehydration, or stains. In principle, freezing and cryoimaging methods avoid artifacts associated with chemical fixation and processing and allow one to visualize the specimen in a condition that is close to its native state. Here we describe a way to use a high voltage electron microscope (HVEM) for the cryoimaging of frozen hydrated PTK1 cells.PTK1 cells were cultured on formvar-coated, carbon stabilized gold grids. After three days in culture, the grids were removed from the culture medium and blotted in a humidity chamber at 35° C. In some instances, the grids were rinsed briefly in 0.16 M ammonium acetate buffer (pH 7.2) prior to blotting. After blotting, the grids were transferred to a plunging apparatus and plunged into liquid ethane held directly above its freezing point. The plunging apparatus consists of a vertical slide rail that guides the fall of a mounted pair of forceps that clamp the specimen. The forceps are surrounded by a plexiglass humidity chamber mounted over a dewar of liquid nitrogen containing an ethane chamber. After freezing, the samples were transferred to liquid nitrogen and viewed in a JEOL JEM 1000 equipped with a top entry cold stage designed and built by Mr. George Wray (Univ. Colorado). The samples were routinely exposed to electron doses of 1 e/Å2/sec, and viewed at a temperature of −150° C. A GATAN video system was used to enhance contrast and to estimate the correct amount of underfocus needed to obtain phase contrast at various magnifications. Low dose micrographs were taken using two second exposures of Kodak 4463 film. The state of the solid water in the specimen was determined by diffraction using a 30/μm field limiting aperture and a camera length of 1 meter.


2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Moch Agus Choiron ◽  
Siti Azizah ◽  
Nafisah Arina Hidayati

Arjowilangun Village is a post-TKI village. People choose to become sheep farmers with less capital than being cattle farmers. Based on the previous discussion, waste management is required to ensure the sustainability of environment in Arjowilangun Village. Processing sheep and agricultural waste program is held by Doktor Mengabdi team. Farmers can learn how to make Bokashi from waste. The method used is composting aerobic or anaerobic starters for composting organic material. A decomposer as collection of several good microbes from fungi work to control pathogenic microbes assists the fermentation process. Based on the results of the pre-test and post-test during the training, it can be showed that understanding of the breeders' potential of the environment is increased.


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