Effect of air drying on bacterial viability: A multiparameter viability assessment

2012 ◽  
Vol 90 (2) ◽  
pp. 86-95 ◽  
Author(s):  
Andreas Nocker ◽  
Priscilla Sossa Fernández ◽  
Roy Montijn ◽  
Frank Schuren
1998 ◽  
Vol 64 (7) ◽  
pp. 2697-2700 ◽  
Author(s):  
P. Lebaron ◽  
P. Catala ◽  
N. Parthuisot

ABSTRACT The effectiveness of SYTOX Green nucleic acid stain for measuring bacterial viability was tested on starved populations ofEscherichia coli and Salmonella typhimurium. This stain underestimates the fraction of dead cells within starved populations containing cells with damaged nucleic acids or membranes. Its application to natural samples should be considered with caution.


Small ◽  
2021 ◽  
pp. 2103765
Author(s):  
Manon Tardif ◽  
Emmanuel Picard ◽  
Victor Gaude ◽  
Jean‐Baptiste Jager ◽  
David Peyrade ◽  
...  

2009 ◽  
Vol 4 (4) ◽  
pp. 424-435 ◽  
Author(s):  
Ido Shamir ◽  
Eran Zahavy ◽  
Yosef Steinberger

Carbon ◽  
2017 ◽  
Vol 120 ◽  
pp. 95-102 ◽  
Author(s):  
Fang Lu ◽  
Yuxiang Song ◽  
Hui Huang ◽  
Yang Liu ◽  
Yijun Fu ◽  
...  

2000 ◽  
Vol 89 (2) ◽  
pp. 370-380 ◽  
Author(s):  
N. Parthuisot ◽  
P. Catala ◽  
K. Lemarchand ◽  
J. Baudart ◽  
P. Lebaron

Author(s):  
T. G. Naymik

Three techniques were incorporated for drying clay-rich specimens: air-drying, freeze-drying and critical point drying. In air-drying, the specimens were set out for several days to dry or were placed in an oven (80°F) for several hours. The freeze-dried specimens were frozen by immersion in liquid nitrogen or in isopentane at near liquid nitrogen temperature and then were immediately placed in the freeze-dry vacuum chamber. The critical point specimens were molded in agar immediately after sampling. When the agar had set up the dehydration series, water-alcohol-amyl acetate-CO2 was carried out. The objectives were to compare the fabric plasmas (clays and precipitates), fabricskeletons (quartz grains) and the relationship between them for each drying technique. The three drying methods are not only applicable to the study of treated soils, but can be incorporated into all SEM clay soil studies.


Author(s):  
Linda M. Sicko ◽  
Thomas E. Jensen

The use of critical point drying is rapidly becoming a popular method of preparing biological samples for scanning electron microscopy. The procedure is rapid, and produces consistent results with a variety of samples. The preservation of surface details is much greater than that of air drying, and the procedure is less complicated than that of freeze drying. This paper will present results comparing conventional air-drying of plant specimens to critical point drying, both of fixed and unfixed material. The preservation of delicate structures which are easily damaged in processing and the use of filter paper as a vehicle for drying will be discussed.


Author(s):  
C.A. Baechler ◽  
W. C. Pitchford ◽  
J. M. Riddle ◽  
C.B. Boyd ◽  
H. Kanagawa ◽  
...  

Preservation of the topographic ultrastructure of soft biological tissues for examination by scanning electron microscopy has been accomplished in the past by using lengthy epoxy infiltration techniques, or dehydration in ethanol or acetone followed by air drying. Since the former technique requires several days of preparation and the latter technique subjects the tissues to great stress during the phase change encountered during air-drying, an alternate rapid, economical, and reliable method of surface structure preservation was developed. Turnbill and Philpott had used a fluorocarbon for the critical point drying of soft tissues and indicated the advantages of working with fluids having both moderately low critical pressures as well as low critical temperatures. Freon-116 (duPont) which has a critical temperature of 19. 7 C and a critical pressure of 432 psi was used in this study.


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