Evaluating the intrinsic capacity of oral bacteria to produce hydrogen peroxide (H2O2) in liquid cultures: Interference by bacterial growth media

Background. Hydrogen peroxide (H2O2) has been used for more than a century clinically to control plaque and gingival inflammation, with unclear supporting evidence. Aim. The aim of the present systematic review of the literature is to assess the effect of mouth rinses with H2O2 on dental plaque, gingival inflammation, and oral microorganisms. Methods. Five databases (PubMed, Scopus, Embase, Cochrane Library, and Web of Science) were searched with the following focused question: what is the effect of hydrogen peroxide, in comparison to chlorhexidine or to a placebo solution, in oral microbiota control, dental plaque, and gingival inflammatory outcomes? Two independent examiners retrieved the articles and evaluated the evidence. Results. The majority of included studies were performed with 1.5% H2O2. Results related to plaque accumulation generally demonstrate a slightly better effect of H2O2 as compared to placebo mouth rinses, however with a lower performance as compared to chlorhexidine. In terms of gingival inflammation, H2O2 performs better than placebo and more clearly demonstrates an anti-inflammation effect. No studies evaluated the effect of H2O2 against viruses or fungi. In terms of bacteria, H2O2 demonstrates an antibacterial effect. Conclusion. Rinsing with H2O2 has the potential to affect plaque, gingivitis, and oral bacteria, as compared to placebo. However, the antibacterial results are not comparable to the performance of chlorhexidine.

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Evaluation of Bacterial and Fungal Growth Media for Ability to Neutralize Cosmetic Preservatives

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/63.6.1200 ◽

1980 ◽

Vol 63 (6) ◽

pp. 1200-1204

Author(s):

Allan M Littell ◽

Michael J Palmieri ◽

Neil B Bisciello

Keyword(s):

Bacterial Growth ◽

Fungal Growth ◽

Tween 80 ◽

Fungal Species ◽

Growth Media ◽

Malt Extract ◽

Bacterial Strains ◽

Standard Methods ◽

Bacterial Cultures ◽

Plate Counts

Abstract Standard methods agar (SMA) and letheen agar (essentially SMA plus lecithin and Tween 80) were compared for bacterial growth and ability to neutralize cosmetic preservatives. Potato dextrose and malt extract agars (each prepared with and without lecithin and Tween 80) were compared with letheen agar and SMA in similar studies with fungi. Twelve bacterial strains, representing 8 species, and 2 fungal species were used as inocula. Plate counts of bacterial cultures (no preservatives present) ranged from 0 to 50% higher on letheen agar than on SMA except for 3 strains of Staphylococcus, which were 8-29% lower. Fungal counts were about the same on all media. Cosmetics (10 g) representing 4 preservative systems (hexachlorophene, benzoin, formaldehyde, and parabens) were inoculated with diluted cultures. Counts at 10−1 and 10−2 dilutions were typically 10-200% higher on letheen agar; however, in one case (benzoin, S. aureus, 10−1) the count was 400 on SMA vs 20 000 on letheen agar. Although differences in fungal counts were not as great, letheen agar partially neutralized the preservatives’ action. Results show that product dilution does not sufficiently reduce the effects of preservative carryover and neutralizers should be incorporated into plating media for this purpose.

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Rapid Evaporative Ionization Mass Spectrometry Imaging Platform for Direct Mapping from Bulk Tissue and Bacterial Growth Media

Analytical Chemistry ◽

10.1021/ac5046752 ◽

2015 ◽

Vol 87 (5) ◽

pp. 2527-2534 ◽

Cited By ~ 66

Author(s):

Ottmar Golf ◽

Nicole Strittmatter ◽

Tamas Karancsi ◽

Steven D. Pringle ◽

Abigail V. M. Speller ◽

...

Keyword(s):

Mass Spectrometry ◽

Bacterial Growth ◽

Mass Spectrometry Imaging ◽

Ionization Mass Spectrometry ◽

Growth Media ◽

Ionization Mass ◽

Direct Mapping ◽

Bulk Tissue

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The effect of hydrogen peroxide on the nutritive value of milk

Journal of Dairy Research ◽

10.1017/s0022029900010761 ◽

1961 ◽

Vol 28 (2) ◽

pp. 177-182 ◽

Cited By ~ 10

Author(s):

Margaret E. Gregory ◽

Kathleen M. Henry ◽

S. K. Kon ◽

J. W. G. Porter ◽

S. Y. Thompson ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Incubation Period ◽

Bacterial Growth ◽

Nutritive Value ◽

Milk Proteins ◽

Bacterial Count ◽

B Complex Vitamins

SummaryTreatment of milk with 0·05% (w/v) H2O2 for 8 h at 24°C had no significant effect on the concentrations of seven of the B-complex vitamins or of the fatsoluble vitamins A and E or of carotene. Rat tests showed that the nutritive value of the milk proteins was slightly reduced. This finding was confirmed by microbiological tests with Streptococcus zymogenes, which also showed that the loss was probably connected with that of methionine. The concentration of H2O2 used was effective in controlling bacterial growth during the incubation period, even in a sample of milk with a high initial bacterial count.

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In Vitro Inhibition of Bacterial Growth Using Different Dental Adhesive Systems

Operative Dentistry ◽

10.2341/06-115 ◽

2007 ◽

Vol 32 (4) ◽

pp. 388-393 ◽

Cited By ~ 12

Author(s):

R. Walter ◽

W. R. Duarte ◽

P. N. R. Pereira ◽

H. O. Heymann ◽

E. J. Swift ◽

...

Keyword(s):

Bacterial Growth ◽

Clinical Relevance ◽

Antibacterial Effect ◽

Oral Bacteria ◽

Adhesive Systems ◽

Dental Adhesive ◽

In Vitro Inhibition

Clinical Relevance All materials tested, especially iBond, have a potential long-term antibacterial effect against the oral bacteria tested.

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Antimicrobial Effects of Equine Platelet Lysate

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.703414 ◽

2021 ◽

Vol 8 ◽

Author(s):

Julie Gordon ◽

Sonsiray Álvarez-Narváez ◽

John F. Peroni

Keyword(s):

Bacterial Growth ◽

Antimicrobial Properties ◽

Normal Growth ◽

Platelet Lysate ◽

Resistant Bacteria ◽

Growth Media ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

E Coli

The development of antimicrobial resistant bacteria and the lack of novel antibiotic strategies to combat those bacteria is an ever-present problem in both veterinary and human medicine. The goal of this study is to evaluate platelet lysate (PL) as a biological alternative antimicrobial product. Platelet lysate is an acellular platelet-derived product rich in growth factors and cytokines that is manufactured via plateletpheresis and pooled from donor horses. In the current study, we sought to define the antimicrobial properties of PL on select gram-positive and gram-negative bacteria. Results from an end-point in vitro assay showed that PL did not support bacterial growth, and in fact significantly reduced bacterial content compared to normal growth media. An in vitro assay was then utilized to further determine the effects on bacterial growth dynamics and showed that all strains exhibited a slower growth rate and lower yield in the presence of PL. The specific effects of PL were unique for each bacterial strain: E. coli and P. aeruginosa growth was affected in a concentration-dependent manner, such that higher amounts of PL had a greater effect, while this was not true for S. aureus or E. faecalis. Furthermore, the onset of exponential growth was delayed for E. coli and P. aeruginosa in the presence of PL, which has significant clinical implications for developing a dosing schedule. In conclusion, our findings demonstrate the potential value of PL as a broad-spectrum antimicrobial that would offer an alternative to traditional antibiotics for the treatment of bacterial infection in equine species.

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Stability of β-lactam antibiotics in bacterial growth media

10.1101/2020.04.15.044123 ◽

2020 ◽

Cited By ~ 1

Author(s):

Rebecca Brouwers ◽

Hugh Vass ◽

Angela Dawson ◽

Tracey Squires ◽

Sharareh Tavaddod ◽

...

Keyword(s):

Aqueous Solution ◽

Minimum Inhibitory Concentration ◽

Growth Medium ◽

Bacterial Growth ◽

Half Life ◽

Inhibitory Concentration ◽

Luria Bertani ◽

Growth Media ◽

Rapid Degradation ◽

Degradation Rates

AbstractLaboratory assays such as MIC tests assume that antibiotic molecules are stable in the chosen growth medium - but rapid degradation has been observed for antibiotics including β-lactams under some conditions in aqueous solution. Degradation rates in bacterial growth medium are less well known. Here, we develop a ‘delay time bioassay’ that provides a simple way to estimate antibiotic stability in bacterial growth media. We use the bioassay to measure degradation half-lives of the β-lactam antibiotics mecillinam, aztreonam and cefotaxime in widely-used bacterial growth media based on MOPS and Luria-Bertani (LB) broth. We find that mecillinam degradation can occur rapidly, with a half-life as short as 2 hours in MOPS medium at 37°C and pH 7.4, and 4-5 hours in LB, but that adjusting the pH and temperature can increase its stability to a half-life around 6 hours without excessively perturbing growth. Aztreonam and cefotaxime were found to have half-lives longer than 6 hours in MOPS medium at 37°C and pH 7.4, but still shorter than the timescale of a typical minimum inhibitory concentration (MIC) assay. Taken together, our results suggest that care is needed in interpreting MIC tests and other laboratory growth assays for β-lactam antibiotics, since there may be significant degradation of the antibiotic during the assay.

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ANALISIS VIABILITAS LACTOBACILLUS LACTIS PADA INOVASI MEDIA DASAR PERTUMBUHAN ALTERNATIF DAN MEDIA DASAR PENEPUNGAN BAKTERI ASAM LAKTAT

Jurnal TAMBORA ◽

10.36761/jt.v4i2.635 ◽

2020 ◽

Vol 4 (2) ◽

pp. 16-22

Author(s):

Ani Sulastri ◽

Baso Manguntungi

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Shelf Life ◽

Bacterial Growth ◽

Alternative Media ◽

Plate Count ◽

Growth Media ◽

Media Formulation ◽

The Media ◽

Lactobacillus Lactis

The limited shelf life in a food requires a natural preservative so that the food used is not easily damaged and has a longer shelf life, namely by using lactic acid bacteria (BAL) using alternative media. By using lactic acid bacteria, the time in the storage period food products can be extended. The purpose of this study was to determine the viability of the Lactobacillus lactis bacteria on an alternative growth base media and a media on the media of bacteria. Lactic acid bacteria were rejuvenated and culture propagation of 5 ?l was inoculated into 5 mL of MRSB media. Formulation media used for bacterial growth such as whey tofu + 5% sucrose + 1% urea. The alternative media was incubated for 24 hours. Bacterial growth was observed at 0, 4, 8 and 16 hours using the TPC (Total Plate count) method. Various media Lactobacillus lactis bacterial deposition was grown on MRSB media and dried with freeze dry for 48 hours and the viability of Lactobacillus lactis was tested. The basic growth media that can be used are Lactobacillus lactis bacteria, namely whey tofu + sucrose 5% + urea 1% as well as Lactobacillus lactis viability results in various media which are grown on MRS media and various alternative media shows that the media has a 100% carrageenan composition able to maintain the viability of Lactobacillus lactis cells.

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Impact of thrombocytes, on bacterial growth and antimicrobial activity of selected antibiotics

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-019-03762-1 ◽

2019 ◽

Vol 39 (3) ◽

pp. 593-597 ◽

Cited By ~ 1

Author(s):

Alina Karoline Nussbaumer-Pröll ◽

Sabine Eberl ◽

Birgit Reiter ◽

Thomas Stimpfl ◽

Walter Jäger ◽

...

Keyword(s):

Escherichia Coli ◽

Bacterial Growth ◽

Limiting Factor ◽

Growth Media ◽

Healthy Human ◽

Bacterial Killing ◽

Bacteria Growth ◽

The Impact

AbstractIn vitro pharmacodynamic models are used to optimize in vivo dosing regimens in antimicrobial drug development. One limiting factor of such models is the lack of host factors such as corpuscular blood components as erythrocytes which have already been shown to impact activity of antibiotics and/or growth of the pathogen. However, the impact of thrombocytes has not previously been investigated. We set out to investigate if the addition of thrombocytes (set to physiological concentrations in blood of healthy human, i.e., 5 × 105 thrombocytes/μL standard growth media Mueller Hinton Broth, MHB) has an influence on bacterial growth and on the efficacy of antibiotics against Gram+ and Gram− bacteria. Growth assays and time-killing-curves (TKC) were performed with ATCC-strains of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa in triplicate over 24 h. The same approach was followed for 5 clinical isolates of Escherichia coli. Meropenem, ciprofloxacin, and tigecycline were tested as representatives of broad-spectrum antibiotics, and concentrations several-fold above and below the minimal inhibitory concentration (MIC) were simulated. No significant impact of thrombocytes was found on bacterial growth or antimicrobial stability for the investigated agents. Bacteria reduced thrombocyte content to different degree, indicating direct interaction of pathogens and thrombocytes. Impact on bacterial killing was observed but was not fully reproducible when thrombocytes from different donors where used. While interaction of bacteria and thrombocytes was evident in the present study, interaction between antibiotic activity and thrombocytes seems unlikely. Whether variability was caused by different thrombocyte concentrates needs further investigation.

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Listeria Phage and Phage Tail Induction Triggered by Components of Bacterial Growth Media (Phosphate, LiCl, Nalidixic Acid, and Acriflavine)

Applied and Environmental Microbiology ◽

10.1128/aem.03235-14 ◽

2015 ◽

Vol 81 (6) ◽

pp. 2117-2124 ◽

Cited By ~ 12

Author(s):

Jean-Paul Lemaître ◽

Amandine Duroux ◽

Romain Pimpie ◽

Jean-Marie Duez ◽

Marie-Louise Milat

Keyword(s):

Listeria Monocytogenes ◽

Nalidixic Acid ◽

Bacterial Growth ◽

False Negative ◽

Brain Heart Infusion ◽

False Negative Result ◽

Growth Media ◽

Content Type ◽

Phage Tail ◽

Initial Ph

ABSTRACTThe detection ofListeria monocytogenesfrom food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth ofL. innocuacan occur in samples where both species are present. In this study, we analyzed the induction of phages and phage tails ofListeriaspp. in these media and in two brain heart infusion (BHI) broths (BHIM [bioMérieux] and BHIK [Biokar]) to identify putative effectors. It appears that Na2HPO4at concentrations ranging from 1 to 40 g/liter with an initial pH of 7.5 can induce phage or phage tail production ofListeriaspp., especially with 10 g/liter of Na2HPO4and a pH of 7.5, conditions present in half-Fraser and Fraser broths. Exposure to LiCl in BHIM (18 to 21 g/liter) can also induce phage and phage tail release, but in half-Fraser and Fraser broths, the concentration of LiCl is much lower (3 g/liter). Low phage titers were induced by acriflavine and/or nalidixic acid. We also show that the production of phages and phage tails can occur in half-Fraser and Fraser broths. This study points out that induction of phages and phage tails could be triggered by compounds present in enrichment media. This could lead to a false-negative result for the detection ofL. monocytogenesin food products.

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