scholarly journals TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways

2021 ◽  
Author(s):  
Iva A. Tchasovnikarova ◽  
Sharon K. Marr ◽  
Manashree Damle ◽  
Robert E. Kingston
Keyword(s):  
Cell Lines ◽  
Reporter Cell

Pharmacological characterization of receptor guanylyl cyclase reporter cell lines

2013 ◽  
Vol 698 (1-3) ◽  
pp. 131-136 ◽  
Author(s):  
Frank Wunder ◽  
Annette Woermann ◽  
Andreas Geerts ◽  
Markus Milde
Keyword(s):  
Cell Lines ◽  
Guanylyl Cyclase ◽  
Reporter Cell ◽  
Pharmacological Characterization

scholarly journals Generation of Double-Labeled Reporter Cell Lines for Studying Co-Dynamics of Endogenous Proteins in Individual Human Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (10) ◽  
pp. e13524 ◽  
Author(s):  
Irina Issaeva ◽  
Ariel A. Cohen ◽  
Eran Eden ◽  
Cellina Cohen-Saidon ◽  
Tamar Danon ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cells ◽  
Reporter Cell ◽  
Endogenous Proteins ◽  
Individual Human

scholarly journals Pharmacological characterization of receptor guanylyl cyclase reporter cell lines

2009 ◽  
Vol 9 (S1) ◽  
Author(s):  
Frank Wunder ◽  
Daniel Barufe ◽  
Annette Woermann ◽  
Markus Milde
Keyword(s):  
Cell Lines ◽  
Guanylyl Cyclase ◽  
Reporter Cell ◽  
Pharmacological Characterization

Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands

Luminescence ◽  
2001 ◽  
Vol 16 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Patrick Balaguer ◽  
Anne-Marie Boussioux ◽  
Ediz Demirpence ◽  
Jean-Claude Nicolas
Keyword(s):  
Biological Activity ◽  
Cell Lines ◽  
Nuclear Receptor ◽  
Receptor Ligands ◽  
Reporter Cell ◽  
Nuclear Receptor Ligands

scholarly journals Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro

2014 ◽  
Vol 13 (2) ◽  
pp. 251-261 ◽  
Author(s):  
Dmitry A. Ovchinnikov ◽  
Drew M. Titmarsh ◽  
Patrick R.J. Fortuna ◽  
Alejandro Hidalgo ◽  
Samah Alharbi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Reporter Cell ◽  
Selection Of

Genetic Reporter Cell Lines: Tools for Stem Cell Biology and Drug Discovery

2015 ◽  
pp. 251-260
Author(s):  
Cameron P.J. Hunt ◽  
Bradley Watmuff ◽  
Brigham J. Hartley ◽  
Colin W. Pouton ◽  
John M. Haynes
Keyword(s):  
Stem Cell ◽  
Drug Discovery ◽  
Cell Lines ◽  
Cell Biology ◽  
Stem Cell Biology ◽  
Reporter Cell

scholarly journals Sugar and Polymer Excipients Enhance Uptake and Splice-Switching Activity of Peptide-Dendrimer/Lipid/Oligonucleotide Formulations

Pharmaceutics ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 666
Author(s):  
Osama Saher ◽  
Taavi Lehto ◽  
Olof Gissberg ◽  
Dhanu Gupta ◽  
Oskar Gustafsson ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Transfection Efficiency ◽  
Protein Translation ◽  
Cervical Cancer Cell Line ◽  
Switching Activity ◽  
Formulation Development ◽  
Reporter Cell ◽  
Biodistribution Studies ◽  
Peptide Dendrimer

Non-viral transfection vectors are commonly used for oligonucleotide (ON) delivery but face many challenges before reaching the desired compartments inside cells. With the support of additional compounds, it might be more feasible for a vector to endure the barriers and achieve efficient delivery. In this report, we screened 18 different excipients and evaluated their effect on the performance of peptide dendrimer/lipid vector to deliver single-stranded, splice-switching ONs under serum conditions. Transfection efficiency was monitored in four different reporter cell lines by measuring splice-switching activity on RNA and protein levels. All reporter cell lines used had a mutated human β-globin intron 2 sequence interrupting the luciferase gene, which led to an aberrant splicing of luciferase pre-mRNA and subsidence of luciferase protein translation. In the HeLa Luc/705 reporter cell line (a cervical cancer cell line), the lead excipients (Polyvinyl derivatives) potentiated the splice-switching activity up to 95-fold, compared to untreated cells with no detected cytotoxicity. Physical characterization revealed that lead excipients decreased the particle size and the zeta potential of the formulations. In vivo biodistribution studies emphasized the influence of formulations as well as the type of excipients on biodistribution profiles of the ON. Subsequently, we suggest that the highlighted impact of tested excipients would potentially assist in formulation development to deliver ON therapeutics in pre-clinical and clinical settings.

scholarly journals Cell Cultures for Virology: Usability, Advantages, and Prospects

2020 ◽  
Vol 21 (21) ◽  
pp. 7978
Author(s):  
Alexander A. Dolskiy ◽  
Irina V. Grishchenko ◽  
Dmitry V. Yudkin
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Virus Isolation ◽  
Virus Detection ◽  
Clinical Samples ◽  
Laboratory Setting ◽  
Reporter Cell ◽  
Isolation And Identification ◽  
Reporter Systems ◽  
Reporter Proteins

Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines. These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.

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