Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

AbstractImplant-associated infections still pose serious problems in modern medicine. The development of fabrication processes to generate functional surfaces, which inhibit bacterial attachment, is of major importance. Sharklet™-like as well as grooves and grid micro-structures having similar dimensions were fabricated on the common implant material titanium by ultra-short pulsed laser ablation. Investigations on the biofilm formation of

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Bond Strengthening in Oral Bacterial Adhesion to Salivary Conditioning Films

Applied and Environmental Microbiology ◽

10.1128/aem.01119-08 ◽

2008 ◽

Vol 74 (17) ◽

pp. 5511-5515 ◽

Cited By ~ 40

Author(s):

Henny C. van der Mei ◽

Minie Rustema-Abbing ◽

Joop de Vries ◽

Henk J. Busscher

Keyword(s):

Bacterial Adhesion ◽

Biofilm Formation ◽

Specific Interactions ◽

Adhesion Forces ◽

Bacterial Strains ◽

Bacterial Interactions ◽

Initial Adhesion ◽

Conditioning Film ◽

Interacting Surfaces ◽

Conditioning Films

ABSTRACT Transition from reversible to irreversible bacterial adhesion is a highly relevant but poorly understood step in initial biofilm formation. We hypothesize that in oral biofilm formation, irreversible adhesion is caused by bond strengthening due to specific bacterial interactions with salivary conditioning films. Here, we compared the initial adhesion of six oral bacterial strains to salivary conditioning films with their adhesion to a bovine serum albumin (BSA) coating and related their adhesion to the strengthening of the binding forces measured with bacteria-coated atomic force microscopy cantilevers. All strains adhered in higher numbers to salivary conditioning films than to BSA coatings, and specific bacterial interactions with salivary conditioning films were accompanied by stronger initial adhesion forces. Bond strengthening occurred on a time scale of several tens of seconds and was slower for actinomyces than for streptococci. Nonspecific interactions between bacteria and BSA coatings strengthened twofold faster than their specific interactions with salivary conditioning films, likely because specific interactions require a closer approach of interacting surfaces with the removal of interfacial water and a more extensive rearrangement of surface structures. After bond strengthening, bacterial adhesion forces with a salivary conditioning film remained stronger than those with BSA coatings.

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A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation

Sensors ◽

10.3390/s21082771 ◽

2021 ◽

Vol 21 (8) ◽

pp. 2771

Author(s):

Tobias Wieland ◽

Julia Assmann ◽

Astrid Bethe ◽

Christian Fidelak ◽

Helena Gmoser ◽

...

Keyword(s):

Antimicrobial Peptides ◽

Real Time ◽

Bacterial Adhesion ◽

Biofilm Formation ◽

Pathogenic Bacteria ◽

Bovine Mastitis ◽

Inhibitory Effect ◽

Thermal Sensor ◽

Bacterial Cells ◽

Static Conditions

The increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm formation, which starts out as bacterial adhesion, poses additional challenges for antibiotics targeting bacterial cells. The objective of this study was to establish a real-time method for the monitoring of the inhibition of (a) bacterial adhesion to a defined substrate and (b) biofilm formation by AMPs using an innovative thermal sensor. We provide evidence that the thermal sensor enables continuous monitoring of the effect of two potent AMPs, protamine and OH-CATH-30, on surface colonization of bovine mastitis-associated Escherichia (E.) coli and Staphylococcus (S.) aureus. The bacteria were grown under static conditions on the surface of the sensor membrane, on which temperature oscillations generated by a heater structure were detected by an amorphous germanium thermistor. Bacterial adhesion, which was confirmed by white light interferometry, caused a detectable amplitude change and phase shift. To our knowledge, the thermal measurement system has never been used to assess the effect of AMPs on bacterial adhesion in real time before. The system could be used to screen and evaluate bacterial adhesion inhibition of both known and novel AMPs.

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Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering

International Journal of Implant Dentistry ◽

10.1186/s40729-020-00261-3 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Daphne de Camargo Reis Mello ◽

Lais Morandini Rodrigues ◽

Fabia Zampieri D’Antola Mello ◽

Thais Fernanda Gonçalves ◽

Bento Ferreira ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Cell Differentiation ◽

Stromal Cells ◽

Biofilm Formation ◽

Bone Marrow Stromal Cells ◽

Pure Titanium ◽

Marrow Stromal Cells ◽

The Other ◽

Cell Based Therapy

Abstract Background An effective biomaterial for bone replacement should have properties to avoid bacterial contamination and promote bone formation while inducing rapid cell differentiation simultaneously. Bone marrow stem cells are currently being investigated because of their known potential for differentiation in osteoblast lineage. This makes these cells a good option for stem cell-based therapy. We have aimed to analyze, in vitro, the potential of pure titanium (Ti), Ti-35Nb-7Zr alloy (A), niobium (Nb), and zirconia (Zr) to avoid the microorganisms S. aureus (S.a) and P. aeruginosa (P.a). Furthermore, our objective was to evaluate if the basic elements of Ti-35Nb-7Zr alloy have any influence on bone marrow stromal cells, the source of stem cells, and observe if these metals have properties to induce cell differentiation into osteoblasts. Methods Bone marrow stromal cells (BMSC) were obtained from mice femurs and cultured in osteogenic media without dexamethasone as an external source of cell differentiation. The samples were divided into Ti-35Nb-7Zr alloy (A), pure titanium (Ti), Nb (niobium), and Zr (zirconia) and were characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). After predetermined periods, cell interaction, cytotoxicity, proliferation, and cell differentiation tests were performed. For monotypic biofilm formation, standardized suspensions (106 cells/ml) with the microorganisms S. aureus (S.a) and P. aeruginosa (P.a) were cultured for 24 h on the samples and submitted to an MTT test. Results All samples presented cell proliferation, growth, and spreading. All groups presented cell viability above 70%, but the alloy (A) showed better results, with statistical differences from Nb and Zr samples. Zr expressed higher ALP activity and was statistically different from the other groups (p < 0.05). In contrast, no statistical difference was observed between the samples as regards mineralization nodules. Lower biofilm formation of S.a and P.a. was observed on the Nb samples, with statistical differences from the other samples. Conclusion Our results suggest that the basic elements present in the alloy have osteoinductive characteristics, and Zr has a good influence on bone marrow stromal cell differentiation. We also believe that Nb has the best potential for reducing the formation of microbial biofilms.

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The Effect of Curcumin against In Vitro Adhesion of Implant Device-Associated Bacteria on Nanosized Titanium Dioxide

Journal of Nano Research ◽

10.4028/www.scientific.net/jnanor.23.83 ◽

2013 ◽

Vol 23 ◽

pp. 83-90

Author(s):

Seung Han Oh ◽

In Young Na ◽

Kyoung Hee Choi

Keyword(s):

Titanium Dioxide ◽

Bacterial Adhesion ◽

Curcuma Longa ◽

Human Tooth ◽

Bacterial Strains ◽

Associated Bacteria ◽

Tooth Surfaces ◽

Titanium Surfaces ◽

Main Component

Although titanium dioxide (TiO2) is an implantable biomaterial with its antibacterial activity, infection on TiO2 surfaces remains a problem for medical settings. According to our previous studies, curcumin, the main component of turmeric (Curcuma longa), partially hindered the attachment of Streptococcus mutans to human tooth surfaces. Therefore, it was examined whether several implant device-associated bacteria were able to adhere to nanosized TiO2 surfaces. In addition, the effect of curcumin on the bacterial adhesion was investigated. Bacterial strains were cultured on pure Ti and TiO2 surfaces with various nanotube sizes in the absence or presence of curcumin and observed by scanning electron microscopy. Consequently, most bacteria adhered to Ti and TiO2 surfaces. However, curcumin increased the adhesion of bacteria including S. mutans. The results suggest that bacterial adhesion to implant titanium surfaces can be augmented via curcumin ingestion.

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Bacterial adhesion to collagens: implications for biofilm formation and disease progression in the oral cavity

Critical Reviews in Microbiology ◽

10.1080/1040841x.2021.1944054 ◽

2021 ◽

pp. 1-13

Author(s):

Simón Álvarez ◽

Camila Leiva-Sabadini ◽

Christina M. A. P. Schuh ◽

Sebastian Aguayo

Keyword(s):

Oral Cavity ◽

Disease Progression ◽

Bacterial Adhesion ◽

Biofilm Formation

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Monolithic Ceramics: Effect of Finishing Techniques on Surface Properties, Bacterial Adhesion and Cell Viability

Operative Dentistry ◽

10.2341/17-011-l ◽

2018 ◽

Vol 43 (3) ◽

pp. 315-325 ◽

Cited By ~ 20

Author(s):

AMO Dal Piva ◽

LPC Contreras ◽

FC Ribeiro ◽

LC Anami ◽

SEA Camargo ◽

...

Keyword(s):

Mtt Assay ◽

Bacterial Adhesion ◽

Biofilm Formation ◽

Nonparametric Tests ◽

Lithium Silicate ◽

Gingival Fibroblasts ◽

Sem Images ◽

High Profile ◽

Colony Forming Units ◽

Monolithic Ceramics

SUMMARY Introduction: This study evaluated the morphology, biofilm formation, and viability of human gingival fibroblasts in contact with two monolithic ceramics after two different finishing techniques: polishing or glazing. For this, 92 blocks (4.5 × 4.5 × 1.5 mm) of each ceramic were made using high translucency zirconia partially stabilized by yttrium (YZHT) and lithium silicate reinforced by zirconium (ZLS). Methods and Materials: Blocks were sintered and then divided into glazing (g) or polishing (p) surface finish. Surface roughness (Ra and RSm) was evaluated through a contact rugosimeter and profilometry. Specimens were contaminated for heterotypic biofilm formation with Streptococcus mutans, Streptococcus sanguinis and Candida albicans for 16 hours. Biofilm was quantified by counting the colony forming units (CFU/mL) and analyzed by scanning electron microscopy (SEM). Fibroblast viability was evaluated by MTT assay. Surface free energy (SFE) was also determined. Roughness data were evaluated using nonparametric tests, while SFE, MTT and CFU results were evaluated by analysis of variance and Tukey test, and MTT data were also submitted to t-test (all, α=0.05). Results: Results showed that polished samples presented a lower high profile mean (p<0.001); however, YZHTg presented less space between defects (p=0.0002). SFE showed that YZHT presented higher SFE than ZLS. Profilometry evidenced more homogeneity on polished surfaces. The interaction of finishing technique and microorganisms influenced the CFU (p=0.00). MTT assay demonstrated initial severe cytotoxic behavior for polished surfaces. SEM images showed homogeneous surfaces, except for glazed YZHT. Conclusion: Glazed surfaces have a greater roughness and tend to accumulate more biofilm. Polished surfaces have higher SFE; however, they are temporarily cytotoxic.

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Antimicrobial Resistance and Biofilm Formation of Pseudomonas aeruginosa

The International Arabic Journal of Antimicrobial Agents ◽

10.3823/846 ◽

2020 ◽

Vol 10 (2) ◽

Author(s):

Abdelraouf A Elmanama ◽

Suhaila Al-Sheboul ◽

Renad I Abu-Dan

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Quorum Sensing ◽

Bacterial Adhesion ◽

Biofilm Formation ◽

Bacterial Infections ◽

Developmental Stages ◽

Infection Site ◽

Major Virulence Factor

Abstract Pseudomonas aeruginosa threatens patient’s care. It is considered as the most complicated health care associated pathogen to be eliminated from infection site. The biofilm forming ability of P. aeruginosa, being a major virulence factor for most pathogenic microorganism, protects it from host immunity and contribute to antibiotic resistance of this organism. It is estimated that about 80% of infectious diseases are due to biofilm mode of growth. Biofilm forming ability of bacteria imparts antimicrobial resistance that leads to many persistent and chronic bacterial infections. The world is becoming increasingly under the threat of entering the “post-antibiotic era”, an era in which the rate of death from bacterial infections is higher than from cancer. This review focus on P. aeruginosa biofilm forming ability; definition, developmental stages, and significance. In addition, the quorum sensing and the antibiotic resistance of this pathogen is discussed. Keywords: Biofilm; bacterial adhesion; Pseudomonas aeruginosa; antimicrobial resistance; quorum sensing.

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