Colonic stem cells mediated drug delivery to treat ulcerative colitis

Abstract Background Muscle stem cells (MuSCs) are absolutely required for the formation, repair, and regeneration of skeletal muscle tissue. Increasing evidence demonstrated that tissue stem cells, especially mesenchymal stem cells (MSCs), can exert therapeutic effects on various degenerative and inflammatory disorders based on their immunoregulatory properties. Human mesenchymal stem cells (hMSCs) treated with interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were reported to possess anti-inflammatory functions by producing TNF-stimulated gene 6 (TSG-6). However, whether human muscle stem cells (hMuSCs) also possess TSG-6 mediated anti-inflammatory functions has not been explored. Methods The ulcerative colitis mouse model was established by subjecting mice to dextran sulfate sodium (DSS) in drinking water for 7 days. hMuSCs were pretreated with IFN-γ and TNF-α for 48 h and were then transplanted intravenously at day 2 of DSS administration. Body weights were monitored daily. Indoleamine 2,3-dioxygenase (IDO) and TSG-6 in hMuSCs were knocked down with short hairpin RNA (shRNA) and small interfering RNA (siRNA), respectively. Colon tissues were collected for length measurement and histopathological examination. The serum level of IL-6 in mice was measured by enzyme-linked immunosorbent assay (ELISA). Real-time PCR and Western blot analysis were performed to evaluate gene expression. Results hMuSCs treated with inflammatory factors significantly ameliorated inflammatory bowel disease (IBD) symptoms. IDO and TSG-6 were greatly upregulated and required for the beneficial effects of hMuSCs on IBD. Mechanistically, the tryptophan metabolites, kynurenine (KYN) or kynurenic acid (KYNA) produced by IDO, augmented the expression of TSG-6 through activating their common receptor aryl hydrocarbon receptor (AHR). Conclusion Inflammatory cytokines-treated hMuSCs can alleviate DSS-induced colitis through IDO-mediated TSG-6 production.

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Recent Advances in Nanoparticle-Mediated Treatment of Inflammatory Bowel Diseases

Applied Sciences ◽

10.3390/app11010438 ◽

2021 ◽

Vol 11 (1) ◽

pp. 438

Author(s):

Andreea Nedelcu ◽

Ofelia Mosteanu ◽

Teodora Pop ◽

Teodora Mocan ◽

Lucian Mocan

Keyword(s):

Ulcerative Colitis ◽

Drug Delivery ◽

Inflammatory Bowel Disease ◽

Drug Therapy ◽

Immune Responses ◽

Biomedical Engineering ◽

Target Therapy ◽

Incidence Rates ◽

Bowel Diseases ◽

Inflammatory Bowel

There have been continuous advances in nanoscience since the beginning of the 21st century, and the emerging field of computational nanomedicine, the development of nanomaterial-based sensors or the prominent biomedical engineering applications should be mentioned. Intestinal disorders causing prolonged inflammation of the digestive tract, largely known as inflammatory bowel disease (IBD), include Crohn’s disease (CD) and ulcerative colitis (UC), have seen a significant increase in incidence rates. Nanoparticle-based approaches to locally target therapy could help regulate immune responses and act as an anti-inflammatory in individual patients diagnosed with IBD. The results of the paper emphasize the major role that nanoparticle-mediated drug delivery has in IBD treatment, giving IBD patients in remission the chance for a more effective drug therapy with a decreased medication load.

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Evaluation of osteogenesis and angiogenesis of icariin loaded on micro/nano hybrid structured hydroxyapatite granules as a local drug delivery system for femoral defect repair

Journal of Materials Chemistry B ◽

10.1039/c5tb00621j ◽

2015 ◽

Vol 3 (24) ◽

pp. 4871-4883 ◽

Cited By ~ 20

Author(s):

Yuqiong Wu ◽

Lunguo Xia ◽

Yuning Zhou ◽

Wudi Ma ◽

Na Zhang ◽

...

Keyword(s):

Drug Delivery ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Drug Delivery System ◽

Local Drug Delivery ◽

Bone Mesenchymal Stem Cells ◽

Femoral Defect ◽

Defect Repair ◽

Local Drug Delivery System

Icariin has been identified to promote osteogenic differentiation of bone mesenchymal stem cells (BMSCs).

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Signal regulation genes screening in colorectal epithelial cells differentiated from mesenchymal stem cells after transplantation in rat with ulcerative colitis

Cytotherapy ◽

10.1016/j.jcyt.2013.01.072 ◽

2013 ◽

Vol 15 (4) ◽

pp. S20

Author(s):

Y. Wei

Keyword(s):

Ulcerative Colitis ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Epithelial Cells ◽

Signal Regulation

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Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165by Ultrasound-Targeted Microbubble Destruction

Journal of Biomedicine and Biotechnology ◽

10.1155/2011/839653 ◽

2011 ◽

Vol 2011 ◽

pp. 1-4 ◽

Cited By ~ 5

Author(s):

Zhaoxia Pu ◽

Xiangdong You ◽

Qiyuan Xu ◽

Feng Gao ◽

Xiaojie Xie ◽

...

Keyword(s):

Drug Delivery ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Protein Expression ◽

Specific Gene ◽

Elisa Kit ◽

The Third ◽

Marrow Mesenchymal Stem Cells ◽

Organ Specific ◽

A New Technique

Ultrasound-targeted microbubble destruction (UTMD) has been proposed as a new technique for organ-specific gene transfer and drug delivery. This study was performed to investigate the effect of UTMD on marrow mesenchymal stem cells (MSCs) transfected with pcDNA3.1−-hVEGF165.pcDNA3.1−-hVEGF165were transfected into the third passage of MSCs, with or without UTMD under different ultrasound conditions. Protein expression was quantified by hVEGF165-ELISA kit after transfection for 24, 48, and 72 hours. UTMD-mediated transfection of MSCs yielded a significant protein expression. UTMD of mechanic index (MI) 0.6 for 90 seconds led to the highest level of protein expression.

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