Red blood cell biomimetic nanoparticle with anti-inflammatory, anti-oxidative and hypolipidemia effect ameliorated atherosclerosis therapy

2022 ◽  
pp. 102519
Author(s):  
Xiaoyu Liang ◽  
Huiyang Li ◽  
Aiai Zhang ◽  
Xinxin Tian ◽  
Haoyang Guo ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Anti Inflammatory

scholarly journals ANTI-INFLAMMATORY STUDY OF ANREDERA CORDIFOLIA LEAVES AND CENTELLA ASIATICA HERBS AND ITS COMBINATIONS USING HUMAN RED BLOOD CELL-MEMBRANE STABILIZATION METHOD

2016 ◽  
Vol 9 (5) ◽  
pp. 78 ◽  
Author(s):  
Entris Sutrisno ◽  
Ketut Adnyana I ◽  
Elin Yulinah Sukandar ◽  
Irda Fidrianny ◽  
Widhya Aligita
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Ethyl Alcohol ◽  
Red Blood Cell ◽  
Centella Asiatica ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Alcohol Extract ◽  
Red Blood Cell Membrane ◽  
Anti Inflammatory

ABSTRACTObjective: Inflammation is body reactions in response to tissue injury and infection. In 2011, non-steroidal anti-inflammatory drug (NSAID) was thehighest demand drug in Indonesia. However, long-term treatment using NSAID can cause several side effects to cardiovascular and digestive system.This research aimed to investigate anti-inflammatory properties of binahong leaves (Anredera cordifolia) and pegagan herbs (Centella asiatica).Methods: Ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs was evaluated for its anti-inflammatory properties using human redblood cell (RBC) – membrane stabilization assay. The extract concentrations used in this study was 100, 200, 400, and 800-ppm, and apigenin andasiaticoside concentration were 1, 2, 3, 4, 5, 6, 10, and 100 ppm. Diclofenac natrium (DN) was used as a standard drug.Results: The results showed that A. cordifolia extract (ACE) alone, C. asiatica extract (CAE) alone, and the combination of ACE and CAE could inhibit thehemolysis of RBC in hypotonic solution. The optimum concentration for ACE alone was 100 ppm; for CAE alone was 400 ppm; and for the combinationof ACE and CAE was 50 ppm and 50 ppm, respectively. Apigenin and asiaticoside in concentration of 1-10 ppm showed more than 97% inhibition ofhemolysis. DN as a standard drug showed optimum inhibition at concentration of 400 ppm.Conclusion: The ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs showed anti-inflammatory activity, both as a single treatment or ascombinations, and apigenin and asiaticoside were responsible for anti-inflammatory activity in C. asiatica.Keywords: Anti-inflammation, Human red blood cell – membrane stabilization, Anredera cordifolia, Centella asiatica.

scholarly journals UJI AKTIVITAS ANTIINFLAMASI INFUSA BUNGA PUKUL EMPAT SECARA IN VITRO

2021 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
Hidayatul Ihsan ◽  
Iman Surya Pratama ◽  
Nisa Isnaeni Hanifa
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Test Group ◽  
Inflammatory Activity ◽  
Test Results ◽  
Membrane Stabilization ◽  
Red Blood Cell Membrane ◽  
Mirabilis Jalapa ◽  
Anti Inflammatory

The stew of Mirabilis jalapa L. flower has been used traditionally as an anti-inflammatory. Phytochemical screening of Mirabilis jalapa L. flower showed flavonoid content which is known to have anti-inflammatory activity. This study aims to determine the anti-inflammatory activity of Mirabilis jalapa L. flower infusion using the red blood cell membrane stabilization method by inducing hypotonicity and heat. The test group consisted of Mirabilis jalapa L. flower infusion with a concentration of 2.5%; 5%; and 10% w / v, positive control (diclofenac sodium 0.01% w / v and aspirin 0.01% w / v), and negative control (distilled water). The anti-inflammatory activity of Mirabilis jalapa L. flower infusion was determined by the percentage of membrane stabilization and inhibition of red blood cell hemolysis. Data from the test results were statistically analyzed both parametric and nonparametric with SPSS. Mirabilis jalapa L. flower infusion contains flavonoids, tannins and terpenoids. The test results showed that the 10% concentration of Mirabilis jalapa L. flower infusion had the largest percentage of red blood cell hemolysis protection on hypotonicity induction, that is 99.50% (p>0.05) and the largest percentage of hemolysis inhibition on heat induction, that is 50.27% ( p≤0.05). From these results, it can be concluded that the Mirabilis jalapa L. flower infusion has potential as an anti-inflammatory by stabilizing the red blood cell membrane.

scholarly journals Comparative Studies on Anti-Inflammatory, Antioxidant and Antimutagenic Activities of Crassocephalum crepidioides (Bent) Leaf Cold and Hot Water Extracts

2019 ◽  
pp. 1-12 ◽  
Author(s):  
B. A. Akinpelu ◽  
A. Godwin ◽  
T. Gbadegesin ◽  
N. Ajakaye ◽  
S. E. Omotosho ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Radical Scavenging ◽  
Hot Water ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Aim: To investigate the anti-inflammatory, anti-oxidant and genotoxicity activities of Crassocephalum crepidioides leaf. Study Design: Comparative investigations of the medicinal value and toxicity profile of cold water (CW) and hot water (HW) extracts of C. crepidioides leaf. Place and Duration of Study: Biochemistry and Molecular Biology Department, Obafemi Awolowo University, Ile-Ife. January 2015-October 2016. Materials and Methods: CW and HW of C. crepidioides were analyzed for anti-inflammatory activity via red blood cell membrane stabilization technique and in vitro methods using DPPH radical scavenging activity, thiobarbituric acid-reactive substances (TBARS), ferric reducing antioxidant power (FRAP) and inhibition of oxidative haemolysis were employed to evaluate the antioxidant property. Allium cepa chromosomal assay was adopted to investigate the genotoxic effect of the extracts. Total flavonoid and phenolic contents of the extracts were estimated spectrophotometrically. Results: Both extracts stabilized stressed red blood cell membranes with maximum percentage stability of 50.97±0.06 and 90.90±0.02 at 0.5 and 2.0 mg/ml for CW and HW extracts respectively. The CW extract elicited no DPPH radical scavenging (IC50 -0.63±0.02 mg/ml) and lipid peroxidation (IC50 -0.32±0.00) activities. HW extract had IC50 of 0.29±0.02 and 0.17±0.00 mg/ml for DPPH and lipid peroxidation. CW and HW extracts exhibited FRAP activity of 1186.96±0.01 and 1015.54±0.01 µmol AAE/g respectively. CW extract displayed a weaker protection (29.01±0.01%) against oxidative haemolysis compared to HW extract (68.70 ± 0.00%). CW extract contained higher phenolic contents (2.16±0.03 µmolGAE/g extract) while the HW extract contained higher flavonoids (0.61±0.05 µmolQE/g extract). CW and HW extracts inhibited A. cepa root growth to 71.40±0.02 and 59.10±0.02% respectively. A. cepa mitotic index was reduced to 8.85±0.01 and 8.67±0.02 for CW and HW extracts as compared with control (26.62%). Conclusion: The study concluded that consumption of C. crepidioides leaf in cooked form has more medicinal values however, both CW and HW extracts are capable of causing cellular damage at high doses.

Uji Aktivitas Anti Inflamasi Ekstrak Bawang Dayak (Eleutherine Americana L. Merr) terhadap Stabilisasi Membran Sel Darah Merah

2019 ◽  
Vol 2 (1) ◽  
pp. 30
Author(s):  
Roji Septian Hardi ◽  
Slamet Slamet ◽  
Laila Kamilla
Keyword(s):  
Diabetes Mellitus ◽  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Diclofenac Sodium ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Purposive Sampling ◽  
Anti Inflammatory ◽  
Eleutherine Americana

Abstract:  Dayak onion plants (Eleutherine americana L. Merr) is one of the most common herbaceous plants used by the community as atraditional medicinal plants. Benefts of dayak onion as a medicine for various diseases such as breast cancer, diabetes mellitus, lowering hypertension, anti-inflammatory and lowering cholesterol.. Based on the research, Dayak bulb extract contains compounds such as Flavonoid, Phenol and Tanin are known to have anti-inflammatory activity. This study aims to determine the difference of anti-inflammatory activity of Dayak extract (Eleutherine americana L. Merr) compared with diclofenac sodium to stabilization of red blood cell membrane. This research is quasi experimental using stabilization method of red blood cell membrane. This study used purposive sampling technique with Dayak extract on concentration samples 0.005, 0.01, 0.02, 0.04 and 0.08% with the repetition of 5 times. Red blood cell lysis Inhibition induced by hipotonis solutionis used as an anti-inflammatory activity measurements. Anti-inflammatory activity of the extract is then compared to positive control (diclofenac sodium). The result of anti-inflammatory activity test showed that the dayak extract which has the highest anti-inflammatory activity was on the extract of 0.08% concentration which was 72.74%, while the most effective concentration of Dayak extract was at a Concentration of 0,02% that is equal to 59,58% of Tukey’s statistical results showed that 0.02% concentration did not differ signifcantly or identical with positive control (diclofenac sodium) at 0.01% concentration of 60.39% with a sample signifcance value of 0.757 ≥ α 0.05 which means that the onion dayak has potential as an antiinflammatoryAbstrak: Tanaman bawang dayak (Eleutherine americana L. Merr) merupakan salah satu jenis tanaman herbal semusim yang lazim digunakan oleh masyarakat sebagai tanaman obat tradisional. Manfaat tanaman bawang dayak sebagai obat berbagai jenis penyakit seperti kanker payudara, diabetes mellitus, menurunkan hipertensi, antiinflamasi dan menurunkan kadar kolesterol. Berdasarkan penelitian, ekstrak umbi bawang dayak mengandung senyawa berupa Flavonoid, Fenol dan Tanin yang diketahui memiliki aktivitas antiinflamasi. Penelitian ini bertujuan untuk mengetahui perbedaan aktivitas antiinflamasi ekstrak bawang dayak (Eleutherine americana L. Merr) yang dibandingkan dengan natrium diklofenak terhadap stabilisasi membran sel darah merah. Penelitian ini merupakan penelitian eksperimental semu dengan menggunakan metode stabilisasi membran sel darah merah. Penelitian ini menggunakan teknik purposive sampling dengan sampel ekstrak bawang dayak konsentrasi 0,005, 0,01, 0,02, 0,04 dan 0,08% dengan dilakukan pengulangan sebanyak 5. Penghambatan lisis sel darah merah akibat induksi larutan hipotonis digunakan sebagai ukuran aktivitas antiinflamasi. Aktivitas antiinflamasi dari ekstrak tersebut kemudian dibandingkan dengan kontrol positif (natriun diklofenak). Hasil uji aktivitas antiinflamasi menunjukkan bahwa ekstrak bawang dayak yang memiliki aktivitas antiinflamasi paling tinggi adalah pada ekstrak konsentrasi 0,08% yaitu sebesar 72,74%, sedangkan konsentrasi yang paling efektif dari ekstrak bawang dayak adalah pada konsentrasi 0,02% yaitu sebesar 59,58% dari hasil uji statistik Tukey menunjukkan pada konsentrasi 0,02% tidak berbeda secara bermakna atau identik dengan kontrol positif (natrium diklofenak) pada konsentrasi 0,01% yaitu sebesar 60,39% dengan nilai signifkansi sampel 0,757 ≥ 0,05 ini menunjukkan bahwa bawang dayak memiliki potensi sebagai antiinflamasi.

scholarly journals Anti-Inflammatory of Papaya Leaf Extract (Carica Papaya L) Towards Membrane Stabilization of Red Blood Cells

2021 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Laila Kamilla ◽  
Sri Tumpuk ◽  
Maulidiyah Salim
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Methanol Extract ◽  
Diclofenac Sodium ◽  
Positive Control ◽  
Modern Medicine ◽  
Inflammatory Activity ◽  
Quasi Experimental ◽  
Anti Inflammatory ◽  
Red Blood Cell Membranes

Traditional medical plants are known to society long ago. Apart from easily obtained and inexpensive, it can cure diseases with few side effects than modern medicine. Papaya leaves were used not only because of contained various chemical compounds with pharmacological effects but also alkaloids, flavonoids, saponins, and tannins compounds indicated as anti-inflammatory. This study applied a quasi-experimental design to test the papaya leaves anti-inflammatory activity. The red blood cell stabilization method was employed because analogous to the lysosomal membrane affected the inflammatory process. Purposive sampling was used, creating papaya leaves extract of 50 ppm, 100 ppm, 200 ppm, 400 ppm, 600 ppm, and 800 ppm concentration, made 24 total samples by four times replication. Based on the red blood cell lysis inhibition, the anti-inflammatory activity was measured and was compared with the positive control (diclofenac sodium). The papaya methanol extract result showed the highest anti-inflammatory activity at 800 ppm of 74.29%. The most effective concentration was at 200 ppm of 62.19%. Tukey's test showed p1.000 ≥ 0.05, suggesting H0 was accepted. There was no difference between the anti-inflammatory activity of papaya leaf methanol extract and diclofenac sodium showing stabilization of red blood cell membranes, indicating papaya potentially as an anti-inflammatory.

scholarly journals Pro- and Anti-Inflammatory Cytokines during Immune Stimulation: Modulation of Iron Status and Red Blood Cell Profile

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
A. M. Koorts ◽  
P. F. Levay ◽  
P. J. Becker ◽  
M. Viljoen
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Iron Status ◽  
Immune Stimulation ◽  
Reactive Protein ◽  
Cytokine Levels ◽  
Rbc Indices ◽  
Anti Inflammatory ◽  
Cell Profile

Forty-eight patients were subdivided according to C-reactive protein (CRP) levels, resulting in 19 patients with normal (2.8 ± 2.8 mg/L) and 29 with elevated (82.2 ± 76.2 mg/L) CRP levels. The elevated CRP group had iron and red blood cell (RBC) profiles characteristic of chronic immune stimulation (CIS), and the normal CRP group, profiles of true iron deficiency. Normal relationships between storage iron, bioavailable iron, and RBC indices were absent in the elevated CRP group—implying the role of iron as major determinant of the RBC profile to be diminished during CIS. The elevated CRP group had significant increases in proinflammatory cytokines (INF-γ, TNF-α, Il-1β, Il-6, and Il-8). Anti-inflammatory cytokine levels were normal, except for Il-10, supporting previous indications that Il-10 contributes to reducing bioavailable iron. Regression analysis suggested decreases in transferrin to be related to increases in Il-8 and an increase in ferritin to be related to a decrease in Il-12 levels. TGF-βlevels were positively related to transferrin and negatively to ferritin.

scholarly journals Hematological Parameters in Cows in Early Lactation Treated with Ketoprofen and their Relationship with Lipid Mobilization and Ketogenesis

2018 ◽  
Vol 44 (1) ◽  
pp. 6
Author(s):  
Zorana Kovacevic ◽  
Dragica Stojanovic ◽  
Marko Cincovic ◽  
Branislava Belic ◽  
Ivana Davidov ◽  
...  
Keyword(s):  
Blood Cell ◽  
Dairy Cows ◽  
Red Blood Cell ◽  
Mean Platelet Volume ◽  
Hematological Parameters ◽  
Control Group ◽  
Low Grade ◽  
Platelet Volume ◽  
Lipid Mobilization ◽  
Anti Inflammatory

Background: Dairy cows are exposed to numerous hematological and biochemical changes, what is bringing cows into the state of increased metabolic activity and physiological adaptations. These adaptive processes have resulted in increased lipid mobilization and ketogenesis with increased concentration of non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHB). As a non steroidal anti-inflammatory drug, ketoprofen produces anti-inflammatory effects. The main objective of the present study was to found relationship between hematological parameters and ketoprofen administration and high lipid mobilization and ketogenesis in cows treated with ketoprofen immediately after calving.Materials, Methods & Results: Ketoprofen was used (3 mg/kg body weight) intramuscularly for three consecutive days post-partum on 15 cows of Holstein-Friesian breed. Cows of the control group (n = 15) were not treated with ketoprofen. Blood samples were collected at the day of calving, in the first and in the second week after parturition from the coccygeal vein of the both groups. Hematological parameters (erythrocytes, hemoglobin, neutrophils, lymphocytes and mean platelet volume) were determined on the automatic hematological counter. Metabolic parameters (NEFA, BHB) were determined by standard colorimetric kits using a semi-automatic biochemistry analyzer. Student’s t-test was used to determine the influence of the application of ketoprofen to the hematological parameters. The data analysis was performed using SPSS, version 19.0, software package for Microsoft Windows (IBM, Armonk, NY, USA). The results of comparison with (P < 0.05) were considered to represent statistically significant differences. Difference in correlation test between NEFA and BHB and other blood parameters in experimental and control group of cows were determined by the Fischer r-to-z transformation. The research results show that there was a increased red blood cell count and a higher concentration of hemoglobin (P < 0.05), fewer neutrophils (P < 0.01), lower number of lymphocytes (P < 0.01) and fewer mean platelet volume in cows that received ketoprofen after calving than those of the control. Determined hematological parameters and values of NEFA and BHB are in correlation. The correlation coefficients were significantly lower in the experimental group of cows. The intensity of the connection between hematological parameters and value of NEFA and BHB decreases.Discussion: It is well known that dairy cows with excessive adiposity manifest a low-grade inflammation and that elevated NEFA concentrations present positive risk factors for many proinflammatory diseases. Analyzing the results in this examination, it has been noticed that the increase of NEFA and BHB after calving were significantly lower in group of cows treated with ketoprofen. Also, determined hematological parameters and values of NEFA and BHB are in correlation. The intensity of these connection decreases in ketoprofen treated cows. In current study, in cows treated with ketoprofen there is decrease in concentrations of inflammatory mediators such as neutrophils and lymphocytes. Red blood cell and hemoglobin concentration decreased in the peripartal period as a result of inflammation. In cows treated with ketoprofen these concentrations increased. In many inflammatory conditions mean platelets volume is increased while there is a decrease in ketoprofen treated cows. Presented results indicate strong relationship between the concentration of NEFA, as well as BHB and hematological parameters indicating that the dependence of hematological parameters of intensity of lipid mobilization and ketogenesis was significantly lower in cows treated with ketoprofen immediately after calving.

scholarly journals Anti-inflammatory potential of root and stem extracts of Sesbania sesban

2017 ◽  
Vol 6 (11) ◽  
pp. 1790
Author(s):  
Baljinder Singh ◽  
Rajiv Sharma ◽  
Rajiv Sharma ◽  
Kalia A.N. ◽  
Kalia A.N.
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Diclofenac Sodium ◽  
Disease Process ◽  
Sesbania Sesban ◽  
Paw Edema ◽  
Red Blood Cell Membrane ◽  
Anti Inflammatory

Successive extracts of root and stem of Sesbania sesban were investigated for in-vitro and in-vivo anti-inflammatory potential employing human red blood cell membrane stabilization and rats paw edema methods respectively. Currently much interest is being paid in the search of medicinal plants with potent anti-inflammatory activity which may lead to the discovery of new therapeutic entity. The plant based agents are not only used to suppress the inflammation but also used in different disease conditions where the inflammation responses are amplifying the disease process. The potency of the successive extracts of root and stem of Sesbania sesban were compared with standard diclofenac sodium (10 mg/kg/b.w.). The n-butanol, aqueous and ethyl acetate extracts showed the most significant (p<0.01) whereas chloroform and total alkaloidal extracts showed modereate anti-inflammatory effects on membrane stabilizing action on human red blood cell membrane and inhibition of rats paw edema methods.

Sign in / Sign up

Export Citation Format

Share Document