Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat

2004 ◽  
Vol 22 (3) ◽  
pp. 544-551 ◽  
Author(s):  
Moyo C. Kruyt ◽  
Wouter J. A. Dhert ◽  
Huipin Yuan ◽  
Clayton E. Wilson ◽  
Clemens A. van Blitterswijk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Critical Size Defect

Electrical impedance spectroscopy – a potential method for the study and monitoring of a bone critical-size defect healing process treated with bone tissue engineering and regenerative medicine approaches

2016 ◽  
Vol 4 (16) ◽  
pp. 2757-2767 ◽  
Author(s):  
Evgeny Kozhevnikov ◽  
Xiaolu Hou ◽  
Shupei Qiao ◽  
Yufang Zhao ◽  
Chunfeng Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Healing Process ◽  
Potential Method ◽  
Electrical Impedance Spectroscopy ◽  
Critical Size Defect ◽  
Critical Size Defects

The development of strategies of bone tissue engineering and regenerative medicine has been drawing considerable attention to treat bone critical-size defects (CSDs).

scholarly journals 3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Ye Lin Park ◽  
Kiwon Park ◽  
Jae Min Cha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Critical Size ◽  
Current Knowledge ◽  
3D Bioprinting ◽  
The Past ◽  
Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

scholarly journals Vascularization Strategies in Bone Tissue Engineering

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1749
Author(s):  
Filip Simunovic ◽  
Günter Finkenzeller
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Ex Vivo ◽  
Angiogenic Growth Factors ◽  
Hypoxic Conditions ◽  
Surgical Methods ◽  
Artificial Tissue

Bone is a highly vascularized tissue, and its development, maturation, remodeling, and regeneration are dependent on a tight regulation of blood vessel supply. This condition also has to be taken into consideration in the context of the development of artificial tissue substitutes. In classic tissue engineering, bone-forming cells such as primary osteoblasts or mesenchymal stem cells are introduced into suitable scaffolds and implanted in order to treat critical-size bone defects. However, such tissue substitutes are initially avascular. Because of the occurrence of hypoxic conditions, especially in larger tissue substitutes, this leads to the death of the implanted cells. Therefore, it is necessary to devise vascularization strategies aiming at fast and efficient vascularization of implanted artificial tissues. In this review article, we present and discuss the current vascularization strategies in bone tissue engineering. These are based on the use of angiogenic growth factors, the co-implantation of blood vessel forming cells, the ex vivo microfabrication of blood vessels by means of bioprinting, and surgical methods for creating surgically transferable composite tissues.

Bone regenerative engineering via controlled release of simple signaling molecules

2018 ◽  
Author(s):  
◽  
Soheila Aliakbarighavimi
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Calcium Phosphates ◽  
Signaling Molecules ◽  
Engineering Applications ◽  
Regenerative Engineering ◽  
Critical Size Defects

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] More than 1.7 billion people worldwide are suffering from bone defects that are due to trauma or medical conditions such as degenerative diseases. Bone can repair and remodel itself, however, in the case of critical size defects, healing is impossible without intervention. Bone regenerative engineering is a new field that focuses on the development of bone substitutes that can stimulate the body to remodel bone tissue in the defect site, most commonly utilizing calcium phosphates in their design to mimic the inorganic phase of bone. Most of previous research has overlooked that calcium phosphates consist of two non-proteinous signaling molecules calcium ions (Ca2+) and phosphate ions (Pi) which are referred as simple signaling molecules that are osteoinductive in a time-dependent and concentration-dependent manner. Higher concentrations of these ions are not only non-inductive, but are also cytotoxic. In my PhD research, I first identified the therapeutic range of Ca2+ and Pi after which I developed two novel platforms capable of controllably delivering these ions within their inductive therapeutic windows. The first platform was comprised of synthetic, hydrophobic, five-carbon polyesters incorporated with rapid dissoluting monobasic calcium phosphate as a scaffold for critical size defects in long bones. The second platform consisted of natural, hydrophilic, chitosan-based hydrogels incorporated with slow dissoluting dibasic calcium phosphate for the treatment of vertebral compression fractures. While I established a new aspect for controllably delivering Ca2+ and Pi as bioactive additives for different bone tissue engineering applications, we are interested in other simple signaling molecules as well. Moving forward, our research group is interested to investigate the effect of hydrogen sulfide (H2S), hydrogen peroxide (H2O2), and carbon monoxide (CO) as cytoprotective, angiogenic, and neuroinductive simple signaling molecules, respectively. The spatiotemporal delivery of multiple simple signaling molecules can be promising for complex bone tissue engineering applications.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

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