scholarly journals Low-cost internal controls for detection of Giardia cysts in water samples

2019 ◽  
Vol 71 ◽  
pp. 177-179
Author(s):  
Melissa Solano Barquero ◽  
Eric Morales Mora ◽  
Luz Chacón Jiménez ◽  
Erick Cordero Jara ◽  
Liliana Reyes Lizano ◽  
...  
Keyword(s):  
Water Samples ◽  
Low Cost ◽  
Internal Controls ◽  
Giardia Cysts

Cryptosporidium Oocysts and Giardia Cysts on Salad Products Irrigated with Contaminated Water

2010 ◽  
Vol 73 (6) ◽  
pp. 1138-1140 ◽  
Author(s):  
INMACULADA AMORÓS ◽  
JOSÉ L. ALONSO ◽  
GONZALO CUESTA
Keyword(s):  
Water Samples ◽  
Environmental Protection Agency ◽  
Internal Controls ◽  
Protozoan Parasites ◽  
Cryptosporidium Oocysts ◽  
Salad Vegetables ◽  
Texas Red ◽  
Irrigation Waters ◽  
Environmental Protection Agency Method ◽  
Giardia Cysts

A field study in Valencia, Spain, was done to determine the occurrence of Giardia and Cryptosporidium on salad products that are frequently eaten raw, such as lettuces and Chinese cabbage, and in irrigation waters. Four water samples were taken weekly 1 month before harvesting the vegetables. All water samples were analyzed using techniques included in the U.S. Environmental Protection Agency Method 1623. Standard methods for detecting protozoan parasites on salad vegetables are not available. Published techniques for the isolation of parasites from vegetables generally have low and variable recovery efficiencies. In this study, vegetables were analyzed using a recently reported method for detection of Cryptosporidium oocysts and Giardia cysts on salad products. The waters tested were positive for both Cryptosporidium and Giardia. Of 19 salad products studied, we observed Cryptosporidium in 12 samples and Giardia in 10 samples. Recoveries of the Texas Red–stained Cryptosporidium and Giardia, which were used as internal controls, were 24.5% ± 3.5% for Cryptosporidium and 16.7% ± 8.1% for Giardia (n = 8). This study provides data on the occurrence of Cryptosporidium and Giardia in salad products in Spain. The method was useful in the detection of Cryptosporidium oocysts and Giardia cysts on the vegetables tested, and it provides a useful analytical tool for occurrence monitoring.

Occurrences of Giardia cysts in beach water

1998 ◽  
Vol 38 (12) ◽  
pp. 73-76 ◽  
Author(s):  
B. S. W. Ho ◽  
T.-Y Tam
Keyword(s):  
Health Risk ◽  
Water Sample ◽  
Water Samples ◽  
High Concentration ◽  
Potential Health ◽  
Infectious Dose ◽  
Potential Health Risk ◽  
E Coli ◽  
Beach Water ◽  
Giardia Cysts

A total of 64 beach water samples with various bacteriological quality (Grades 1 to 4) were analysed for their bacteriological and parasitological contents (E coli and Giardia cysts respectively). Results indicated that Giardia cysts were detected in less than 10% of the Grade 1 beach water samples with E coli concentrations of <24/100mL. For Grades 2, 3 & 4 beach water samples, Giardia cysts were found, respectively, in 85, 50 and 64% of the samples. Except for one beach water sample which had an unusually high concentration of Giardia cysts (23 cysts/L), they were generally present at moderate concentrations (<10 cysts/L) in all other beach water samples. Despite moderate levels of Giardia cysts present in beach water of different grades, the potential health risk faced by swimmers bathing in local beach water needs to be carefully assessed as Giardia is known to have a low infectious dose.

Modern nanobiotechnologies for efficient detection and remediation of mercury

Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mulayam Singh Gaur ◽  
Rajni Yadav ◽  
Mamta Kushwah ◽  
Anna Nikolaevna Berlina
Keyword(s):  
Heavy Metals ◽  
Water Samples ◽  
Low Cost ◽  
High Sensitivity ◽  
Environmental Water ◽  
Mercury Ions ◽  
Content Type ◽  
Efficient Detection ◽  
Sensitivity And Selectivity ◽  
Selection Of

Purpose This information will be useful in the selection of materials and technology for the detection and removal of mercury ions at a low cost and with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. Design/methodology/approach Different nano- and bio-materials allowed for the development of a variety of biosensors – colorimetric, chemiluminescent, electrochemical, whole-cell and aptasensors – are described. The materials used for their development also make it possible to use them in removing heavy metals, which are toxic contaminants, from environmental water samples. Findings This review focuses on different technologies, tools and materials for mercury (heavy metals) detection and remediation to environmental samples. Originality/value This review gives up-to-date and systemic information on modern nanotechnology methods for heavy metal detection. Different recognition molecules and nanomaterials have been discussed for remediation to water samples. The present review may provide valuable information to researchers regarding novel mercury ions detection sensors and encourage them for further research/development.

scholarly journals Removal of Cyanide-Contaminated Water by Vetiver Grasses

2015 ◽  
Vol 9 (13) ◽  
pp. 252 ◽  
Author(s):  
Piyada Wachirawongsakorn ◽  
Tongsai Jamnongkan ◽  
Mohd Talib Latif
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Sri Lanka ◽  
Removal Efficiency ◽  
Water Samples ◽  
Low Cost ◽  
Contaminated Water ◽  
Vetiver Grass ◽  
Vetiveria Zizanioides ◽  
Concentration Levels

<p>Vetiver grass and it usages have been widely investigated in many researches as the preferred plant species due to its known efficiency, low cost, the ease of availability and spread. This research aimed to use four different vetiver grass (<em>Vetiveria zizanioides</em>) ecotypes to remove cyanide (CN<sup>-</sup>)-contaminated water for improve its quality. Growth capability, tolerance and removal efficiency were evaluated. The results showed that the vetiver grass had a 100% survival rate for one month after planting. Songkhlar3 had the longest leaves, followed by Surat-Thani, Sri Lanka and Monto, respectively. Root lengths of all ecotypes showed no significant differences (p ≤ 0.05). All vetiver grass ecotypes could potentially purify CN<sup>-</sup>-contaminated water at lower concentrations of ≤ 35 mg CN<sup>-</sup>/L. The Monto ecotype had the highest CN<sup>-</sup> removal efficiency at all CN<sup>-</sup> concentration levels, showing 100% CN<sup>-</sup> removal from the 5-45 mg CN<sup>-</sup>/L contaminated water samples within 2-5 weeks growth. The tolerance of vetiver grass to CN<sup>-</sup> was a more important factor than growth rate when selecting a vetiver grass ecotype for CN<sup>-</sup> phytoremediation.</p>

scholarly journals Technical Note: Evaluation of a low-cost evaporation protection method for portable water samplers

2020 ◽  
Author(s):  
Jana von Freyberg ◽  
Julia L. A. Knapp ◽  
Andrea Rücker ◽  
Bjørn Studer ◽  
James W. Kirchner
Keyword(s):  
Water Samples ◽  
Low Cost ◽  
Isotopic Fractionation ◽  
Field Tests ◽  
Isotopic Signature ◽  
Technical Note ◽  
Stable Water Isotopes ◽  
Sample Collection ◽  
Protection Method ◽  
Cost Efficient

Abstract. Automated field sampling of streamwater or precipitation for subsequent analysis of stable water isotopes (2H and 18O) is often conducted with off-the-shelf automated samplers. However, water samples stored in the field for days and weeks in open bottles inside autosamplers undergo isotopic fractionation and vapor mixing, thus altering their isotopic signature. We therefore designed an evaporation protection method which modifies autosampler bottles using a syringe housing and silicone tube, and tested whether this method reduces evaporative fractionation and vapor mixing in water samples stored for up to 24 days in ISCO autosamplers (Teledyne ISCO., Lincoln, US). Laboratory and field tests under different temperature and humidity conditions showed that water samples in bottles with evaporation protection were far less altered by evaporative fractionation and vapor mixing than samples in conventional open bottles. Our design is a cost-efficient approach to upgrade the 1-litre sample bottles of ISCO 6712 Full-size Portable Samplers, allowing secure water sample collection in warm and dry environments. Our design can be readily adapted (e.g., by using a different syringe size) to fit the bottles used by many other field autosamplers.

Occurrence of Cryptosporidium oocysts and Giardia cysts in a conventional water purification plant

2001 ◽  
Vol 43 (12) ◽  
pp. 89-92 ◽  
Author(s):  
A. Hashimoto ◽  
T. Hirata ◽  
S. Kunikane
Keyword(s):  
Water Purification ◽  
Water Samples ◽  
Geometric Mean ◽  
Giardia Cyst ◽  
Raw Water ◽  
Observation Level ◽  
Cryptosporidium Oocysts ◽  
Purification Plant ◽  
Mean Concentration ◽  
Giardia Cysts

A one-year monitoring of Cryptosporidium oocysts and Giardia cysts was conducted at a water purification plant. A total of thirteen 50 L samples of river source water and twenty-six 2,000 L samples of filtered water (treated by coagulation-flocculation, sedimentation and rapid filtration) were concentrated using a hollow fibre ultrafiltration membrane module at a purification plant. Cryptosporidium oocysts were detected in all raw water samples with a geometric mean concentration of 400 oocysts/m3 (range 160-1,500 oocysts/m3). Giardia cysts were detected in 12/13 raw waters (92%) with a geometric mean concentration of 170 cysts/m3 (range 40-580 oocysts/m3). Probability distributions of both Cryptosporidium oocyst and Giardia cyst concentration in raw water were nearly log-normal. In filtered water samples, Cryptosporidium oocysts were detected in 9/26 samples (35%) with a geometric mean concentration of 1.2 oocysts/m3 (range 0.5-8 oocysts/m3) and Giardia cysts in three samples (12%) with 0.8 cysts/m3 (range 0.5-2 oocysts/m3). The estimated removal of Cryptosporidium oocysts and Giardia cysts was, respectively, 2.54 log10 and 2.53 log10 on the basis of geometric means, 3.20 and 3.57 log10 on the basis of 50% observation level and 2.70 and 2.90 log10 on the basis of 90% observation level.

scholarly journals Utility of a PCR-based method for rapid and specific detection of toxigenic Microcystis spp. in farm ponds

2020 ◽  
Vol 32 (3) ◽  
pp. 369-381 ◽  
Author(s):  
Jian Yuan ◽  
Hyun-Joong Kim ◽  
Christopher T. Filstrup ◽  
Baoqing Guo ◽  
Paula Imerman ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Water Samples ◽  
Low Cost ◽  
Farm Animals ◽  
Rrna Gene ◽  
Analytical Sensitivity ◽  
Water Safety ◽  
Farm Ponds ◽  
Pcr Method

Microcystis is a widespread freshwater cyanobacterium that can produce microcystin, a potent hepatotoxin harmful to animals and humans. Therefore, it is crucial to monitor for the presence of toxigenic Microcystis spp. to provide early warning of potential microcystin contamination. Microscopy, which has been used traditionally to identify Microcystis spp., cannot differentiate toxigenic from non-toxigenic Microcystis. We developed a PCR-based method to detect toxigenic Microcystis spp. based on detection of the microcystin synthetase C ( mcyC) gene and 16S rRNA gene. Specificity was validated against toxic and nontoxic M. aeruginosa strains, as well as 4 intergeneric freshwater cyanobacterial strains. Analytical sensitivity was as low as 747 fg/µL genomic DNA (or 3 cells/µL) for toxic M. aeruginosa. Furthermore, we tested 60 water samples from 4 farm ponds providing drinking water to swine facilities in the midwestern United States using this method. Although all water samples were positive for Microcystis spp. (i.e., 16S rRNA gene), toxigenic Microcystis spp. were detected in only 34 samples (57%). Seventeen water samples contained microcystin (0.1–9.1 μg/L) determined with liquid chromatography–mass spectrometry, of which 14 samples (82%) were positive for mcyC. A significant correlation was found between the presence of toxigenic Microcystis spp. and microcystin in water samples ( p = 0.0004). Our PCR method can be a low-cost molecular tool for rapid and specific identification of toxigenic Microcystis spp. in farm ponds, improving detection of microcystin contamination, and ensuring water safety for farm animals.

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