Antifibrotic effects of Hypocrellin A combined with LED red light irradiation on keloid fibroblasts by counteracting the TGF-β/Smad/autophagy/apoptosis signalling pathway

2021 ◽  
Vol 34 ◽  
pp. 102202
Author(s):  
Tianhui Niu ◽  
Yan Tian ◽  
Ying Shi ◽  
Guangjin Guo ◽  
Ying Tong ◽  
...  
Keyword(s):  
Red Light ◽  
Signalling Pathway ◽  
Light Irradiation ◽  
Hypocrellin A ◽  
Keloid Fibroblasts

scholarly journals Antifibrotic activity of Hypocrellin A united LED red light irradiation on Keloid Fibroblasts through counteracting TGF-β/Smad/autophagy/apoptosis signaling pathway

2020 ◽  
Author(s):  
Tianhui Niu ◽  
Yan Tian ◽  
Ying Shi ◽  
Guangjin Guo ◽  
Ying Tong ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Light Emitting Diode ◽  
Red Light ◽  
Systematic Investigation ◽  
Light Irradiation ◽  
Light Emitting ◽  
Hypocrellin A ◽  
Keloid Fibroblast ◽  
Smad Signaling Pathway ◽  
Tgf Β1

Abstract Keloid disease is characterized by abnormal proliferation of fibroblast and continuous deposition of extracellular matrix (ECM) components. More and more attention in dermopathic is paid to photodynamic therapy (PDT) with visible light. The natural photosensitizer Hypocrellin A (HA) is identified to develop a splendid light induced anticancer, antimicrobial and antiviral activity. In this experiment, we investigated the impacts of HA united light-emitting diode (LED) red light irradiation on human keloid fibroblast cells (KFs). Our results showed that HA united red light irradiation treatment (HA-R-PDT) decreased KFs viability, reduced KFs collagen production and ECM accumulation, inhibited cell proliferation, suppressed cell invasion and induced cell apoptosis. Moreover, our observations demonstrated that TGF-β/Smad signaling pathway and autophagy were restrained by HA-R-PDT. TGF-β1 could accommodate autophagy in KFs through both Smad and ERK pathway, while inhibiting autophagy could mediate TGF-β1 level by the negative feedback. Therefore, HA-R-PDT could suppress cell hyper-proliferation, collagen synthesis and ECM accumulation in KFs via regulating TGF-β1-ERK-autophagy-apoptosis signaling pathway. HA-R-PDT deserves systematic investigation as a potential therapeutic strategy for keloid treatment and autophagy might be a promising candidate in the therapy of KFs.

scholarly journals LED and UV Light Utilizing Flavonoids and Limonoids to Investigate the Effect of Post-harvest Light Irradiation

2020 ◽  
Author(s):  
Shengyu Liu
Keyword(s):  
White Light ◽  
Uv Light ◽  
Light Emitting Diode ◽  
Red Light ◽  
Principal Component ◽  
Experimental Period ◽  
Light Irradiation ◽  
Flavonoid Glycosides ◽  
Clear Correlation ◽  
Post Harvest

To investigate the effect of post-harvest light irradiation on the accumulation of flavonoids and limonoids, harvestedNewhall navel oranges were continuously exposed to light-emitting diode (LED) and ultraviolet (UV) light irradiationfor 6 days, and the composition and content of flavonoids and limonoids in the segments were determined usingUPLC-qTOF-MS at 0, 6, and 15 days after harvest. In total, six polymethoxylated flavonoids (PMFs), fiveflavoneO/C-glycosides, seven flavanone-O-glycosides, and three limonoids were identified in the segments. Theaccumulation of these components was altered by light irradiation. Red and blue light resulted in higher levels ofPMFs during exposure periods. The accumulation of PMFs was also significantly induced after white light, UVBand UVC irradiation were removed. Red and UVC irradiation induced the accumulation of flavone and flavanoneglycosides throughout the entire experimental period. Single light induced limonoid accumulation during exposureperiods, but limonoid levels decreased significantly when irradiation was removed. Principal component analysisshowed a clear correlation between PMFs and white light, between flavonoid glycosides and red light and UVC,and between limonoids and UVC. These results suggest that the accumulation of flavonoids and limonoids in citrusis regulated by light irradiation. White light, red light and UVC irradiation might be a good potential method forimproving the nutrition and flavor quality of post-harvest citrus.

Effect of pH on Absorption Spectra of Pea 114 and 121 Kilodalton Phytochromes during and after Red-Light Irradiation

1986 ◽  
Vol 27 (5) ◽  
pp. 765-773 ◽  
Author(s):  
Satoru Tokutomi ◽  
Yasunori Inoue ◽  
Naoki Sato ◽  
Kotaro T. Yamamoto ◽  
Masaki Furuya
Keyword(s):  
Absorption Spectra ◽  
Red Light ◽  
Light Irradiation ◽  
Effect Of Ph

scholarly journals Effect of Post-Harvest LED and UV Light Irradiation on the Accumulation of Flavonoids and Limonoids in the Segments of Newhall Navel Oranges (Citrus sinensis Osbeck)

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1755 ◽  
Author(s):  
Shengyu Liu ◽  
Linping Hu ◽  
Dong Jiang ◽  
Wanpeng Xi
Keyword(s):  
White Light ◽  
Uv Light ◽  
Light Emitting Diode ◽  
Red Light ◽  
Principal Component ◽  
Light Irradiation ◽  
Flavonoid Glycosides ◽  
Clear Correlation ◽  
Post Harvest ◽  
Uv Light Irradiation

To investigate the effect of post-harvest light irradiation on the accumulation of flavonoids and limonoids, harvested Newhall navel oranges were continuously exposed to light-emitting diode (LED) and ultraviolet (UV) light irradiation for 6 days, and the composition and content of flavonoids and limonoids in the segments were determined using UPLC-qTOF-MS at 0, 6, and 15 days after harvest. In total, six polymethoxylated flavonoids (PMFs), five flavone-O/C-glycosides, seven flavanone-O-glycosides, and three limonoids were identified in the segments. The accumulation of these components was altered by light irradiation. Red and blue light resulted in higher levels of PMFs during exposure periods. The accumulation of PMFs was also significantly induced after white light, UVB and UVC irradiation were removed. Red and UVC irradiation induced the accumulation of flavone and flavanone glycosides throughout the entire experimental period. Single light induced limonoid accumulation during exposure periods, but limonoid levels decreased significantly when irradiation was removed. Principal component analysis showed a clear correlation between PMFs and white light, between flavonoid glycosides and red light and UVC, and between limonoids and UVC. These results suggest that the accumulation of flavonoids and limonoids in citrus is regulated by light irradiation. White light, red light and UVC irradiation might be a good potential method for improving the nutrition and flavor quality of post-harvest citrus.

scholarly journals Role of elemental carbon in the photochemical aging of soot

2018 ◽  
Vol 115 (30) ◽  
pp. 7717-7722 ◽  
Author(s):  
Meng Li ◽  
Fengxia Bao ◽  
Yue Zhang ◽  
Wenjing Song ◽  
Chuncheng Chen ◽  
...  
Keyword(s):  
Light Absorption ◽  
Elemental Carbon ◽  
Red Light ◽  
Field Measurements ◽  
Light Irradiation ◽  
Photochemical Oxidation ◽  
Chemical Mechanism ◽  
Strong Light ◽  
Electron Transfer Pathway ◽  
Photochemical Aging

Soot, which consists of organic carbon (OC) and elemental carbon (EC), is a significant component of the total aerosol mass in the atmosphere. Photochemical oxidation is an important aging pathway for soot. It is commonly believed that OC is photoactive but EC, albeit its strong light absorption, is photochemically inert. Here, by taking advantage of the different light absorption properties of OC and EC, we provide direct experimental evidence that EC also plays an important role in the photochemical aging of soot by initiating the oxidation of OC, even under red light irradiation. We show that nascent soot, in addition to undergoing photochemical oxidation under blue light with a wavelength of 440 nm, undergoes similar oxidation under red light irradiation of λ = 648 nm (L648). However, separated OC (extracted from soot by n-hexane) and EC exhibit little reactivity under L648. These observations indicate that EC plays a pivotal role in photoaging of soot by adsorbing light to initiate the oxidation of OC. Comparison of in situ IR spectra and photoelectrochemical behaviors suggests that EC-initiated photooxidation of OC proceeds through an electron transfer pathway, which is distinct from the photoaging induced by light absorption of OC. Since the absorption spectra of EC have a much larger overlap with the solar spectra than those of OC, our results provide insight into the chemical mechanism leading to rapid soot aging by organic species observed from atmospheric field measurements.

scholarly journals Trifluoroethoxy-Coated Subphthalocyanine affects Trifluoromethylation of Alkenes and Alkynes even under Low-Energy Red-Light Irradiation

ChemistryOpen ◽  
2017 ◽  
Vol 6 (2) ◽  
pp. 226-230 ◽  
Author(s):  
Kohei Matsuzaki ◽  
Tomoya Hiromura ◽  
Etsuko Tokunaga ◽  
Norio Shibata
Keyword(s):  
Red Light ◽  
Low Energy ◽  
Light Irradiation

Biological Properties of Nanocrystalline Silicon Particles for Biomedical Applications

2006 ◽  
Vol 958 ◽  
Author(s):  
Keisuke Sato ◽  
Satoshi Yanagisawa ◽  
Akio Funakubo ◽  
Yasuhiro Fukui ◽  
Kenji Hirakuri ◽  
...  
Keyword(s):  
Small Intestine ◽  
Biomedical Applications ◽  
Uv Light ◽  
Red Light ◽  
Nanocrystalline Silicon ◽  
Biological Properties ◽  
Light Irradiation ◽  
Uv Light Irradiation ◽  
Luminescence Peak ◽  
Red Luminescence

ABSTRACTWe have studied the biological properties of nanocrystalline silicon (nc-Si) particles after injection at various places in a mouse. The nc-Si particles with a size of 2.5 nm and a concentration of 1.3 mg/ml were dispersed in a normal saline solution (NSS). The NSS dispersible nc-Si particles were safely injected into the mouse. When the nc-Si particles in the NSS were directly injected into the subcutaneous vein and the coronary artery of the heart by syringe, the condition of bloodstream at each place was confirmed by the red luminescence (peak wavelength at 720 nm) from the nc-Si particles under the ultraviolet (UV) light-irradiation. Moreover, the nc-Si particles in the NSS, which were injected into the vein in the sole, smoothly flowed to the small intestine, and the smooth fluidity of nc-Si particles was also observed for the condition of the peristalsis of the small intestine. The nc-Si particles in the small intestine emitted red light during peristalsis under the UV light-irradiation. The red luminescence at each place was very bright and could be clearly seen with the naked eye. These phenomenons were achieved by the utilization of the harmless material, the formation of nc-Si particles with the single-order-size and the realization of the stable surface modification to the nc-Si particles.

Sign in / Sign up

Export Citation Format

Share Document