Regulation of cell proliferation by NADPH oxidase-mediated signaling: Potential roles in tissue repair, regenerative medicine and tissue engineering

Stem cells have been extensively used in regenerative medicine and tissue engineering; however, they often lose their functionality because of the inflammatory microenvironment. This leads to their poor survival, retention, and engraftment at transplantation sites. Considering the rapid loss of transplanted cells due to poor cell-cell and cell-extracellular matrix (ECM) interactions during transplantation, it has been reasoned that stem cells mainly mediate reparative responses via paracrine mechanisms, including the secretion of extracellular vesicles (EVs). Ameliorating poor cell-cell and cell-ECM interactions may obviate the limitations associated with the poor retention and engraftment of transplanted cells and enable them to mediate tissue repair through the sustained and localized presentation of secreted bioactive cues. Biomaterial-mediated strategies may be leveraged to confer stem cells enhanced immunomodulatory properties, as well as better engraftment and retention at the target site. In these approaches, biomaterials have been exploited to spatiotemporally present bioactive cues to stem cell-laden platforms (e.g., aggregates, microtissues, and tissue-engineered constructs). An array of biomaterials, such as nanoparticles, hydrogels, and scaffolds, has been exploited to facilitate stem cells function at the target site. Additionally, biomaterials can be harnessed to suppress the inflammatory microenvironment to induce enhanced tissue repair. In this review, we summarize biomaterial-based platforms that impact stem cell function for better tissue repair that may have broader implications for the treatment of various diseases as well as tissue regeneration.

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Bioactive Materials for Soft Tissue Repair

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.613787 ◽

2021 ◽

Vol 9 ◽

Cited By ~ 2

Author(s):

Elisa Mazzoni ◽

Maria Rosa Iaquinta ◽

Carmen Lanzillotti ◽

Chiara Mazziotta ◽

Martina Maritati ◽

...

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Regenerative Medicine ◽

Tissue Repair ◽

Soft Tissues ◽

High Efficiency ◽

Calcium Phosphates ◽

Tissue Growth ◽

Tissue Healing ◽

Soft Tissue Engineering

Over the past decades, age-related pathologies have increased abreast the aging population worldwide. The increased age of the population indicates that new tools, such as biomaterials/scaffolds for damaged tissues, which display high efficiency, effectively and in a limited period of time, for the regeneration of the body's tissue are needed. Indeed, scaffolds can be used as templates for three-dimensional tissue growth in order to promote the tissue healing stimulating the body's own regenerative mechanisms. In tissue engineering, several types of biomaterials are employed, such as bioceramics including calcium phosphates, bioactive glasses, and glass–ceramics. These scaffolds seem to have a high potential as biomaterials in regenerative medicine. In addition, in conjunction with other materials, such as polymers, ceramic scaffolds may be used to manufacture composite scaffolds characterized by high biocompatibility, mechanical efficiency and load-bearing capabilities that render these biomaterials suitable for regenerative medicine applications. Usually, bioceramics have been used to repair hard tissues, such as bone and dental defects. More recently, in the field of soft tissue engineering, this form of scaffold has also shown promising applications. Indeed, soft tissues are continuously exposed to damages, such as burns or mechanical traumas, tumors and degenerative pathology, and, thereby, thousands of people need remedial interventions such as biomaterials-based therapies. It is known that scaffolds can affect the ability to bind, proliferate and differentiate cells similar to those of autologous tissues. Therefore, it is important to investigate the interaction between bioceramics and somatic/stem cells derived from soft tissues in order to promote tissue healing. Biomimetic scaffolds are frequently employed as drug-delivery system using several therapeutic molecules to increase their biological performance, leading to ultimate products with innovative functionalities. This review provides an overview of essential requirements for soft tissue engineering biomaterials. Data on recent progresses of porous bioceramics and composites for tissue repair are also presented.

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Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine

Biomolecules ◽

10.3390/biom9090470 ◽

2019 ◽

Vol 9 (9) ◽

pp. 470 ◽

Cited By ~ 38

Author(s):

Bolat Sultankulov ◽

Dmitriy Berillo ◽

Karina Sultankulova ◽

Tursonjan Tokay ◽

Arman Saparov

Keyword(s):

Tissue Engineering ◽

Regenerative Medicine ◽

Tissue Repair ◽

Chemically Modified ◽

Tissue Repair And Regeneration ◽

Current Review ◽

Free Amine ◽

Natural Polysaccharide ◽

Amine Groups ◽

Made In

Over the last few decades, chitosan has become a good candidate for tissue engineering applications. Derived from chitin, chitosan is a unique natural polysaccharide with outstanding properties in line with excellent biodegradability, biocompatibility, and antimicrobial activity. Due to the presence of free amine groups in its backbone chain, chitosan could be further chemically modified to possess additional functional properties useful for the development of different biomaterials in regenerative medicine. In the current review, we will highlight the progress made in the development of chitosan-containing bioscaffolds, such as gels, sponges, films, and fibers, and their possible applications in tissue repair and regeneration, as well as the use of chitosan as a component for drug delivery applications.

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Spatiotemporal Control over Cell Proliferation and Differentiation for Tissue Engineering and Regenerative Medicine Applications Using Silk Fibroin Scaffolds

ACS Applied Bio Materials ◽

10.1021/acsabm.0c00305 ◽

2020 ◽

Vol 3 (6) ◽

pp. 3476-3493 ◽

Cited By ~ 1

Author(s):

Smita Patil ◽

Vartika Dhyani ◽

Tejinder Kaur ◽

Neetu Singh

Keyword(s):

Tissue Engineering ◽

Cell Proliferation ◽

Regenerative Medicine ◽

Silk Fibroin ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation ◽

Spatiotemporal Control

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Engineering Extracellular Vesicles as Nanotherapeutics for Regenerative Medicine

Biomolecules ◽

10.3390/biom10010048 ◽

2019 ◽

Vol 10 (1) ◽

pp. 48 ◽

Cited By ~ 8

Author(s):

Lalithasri Ramasubramanian ◽

Priyadarsini Kumar ◽

Aijun Wang

Keyword(s):

Cell Proliferation ◽

Regenerative Medicine ◽

Extracellular Vesicles ◽

Tissue Repair ◽

Functional Heterogeneity ◽

Isolation And Purification ◽

Natural Properties ◽

New Class ◽

Matrix Organization ◽

Recycled Waste

Long thought of to be vesicles that primarily recycled waste biomolecules from cells, extracellular vesicles (EVs) have now emerged as a new class of nanotherapeutics for regenerative medicine. Recent studies have proven their potential as mediators of cell proliferation, immunomodulation, extracellular matrix organization and angiogenesis, and are currently being used as treatments for a variety of diseases and injuries. They are now being used in combination with a variety of more traditional biomaterials and tissue engineering strategies to stimulate tissue repair and wound healing. However, the clinical translation of EVs has been greatly slowed due to difficulties in EV isolation and purification, as well as their limited yields and functional heterogeneity. Thus, a field of EV engineering has emerged in order to augment the natural properties of EVs and to recapitulate their function in semi-synthetic and synthetic EVs. Here, we have reviewed current technologies and techniques in this growing field of EV engineering while highlighting possible future applications for regenerative medicine.

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Neural stem cell proliferation and differentiation in the conductive PEDOT-HA/Cs/Gel scaffold for neural tissue engineering

Biomaterials Science ◽

10.1039/c7bm00633k ◽

2017 ◽

Vol 5 (10) ◽

pp. 2024-2034 ◽

Cited By ~ 34

Author(s):

Shuping Wang ◽

Shui Guan ◽

Jianqiang Xu ◽

Wenfang Li ◽

Dan Ge ◽

...

Keyword(s):

Tissue Engineering ◽

Cell Proliferation ◽

Stem Cell ◽

Regenerative Medicine ◽

Neural Tissue ◽

Neural Tissue Engineering ◽

Stem Cell Proliferation ◽

Neural Stem Cell Proliferation ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

Engineering scaffolds with excellent electro-activity is increasingly important in tissue engineering and regenerative medicine.

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Effects of Electrical Stimulation on Stem Cells

Current Stem Cell Research & Therapy ◽

10.2174/1574888x15666200129154747 ◽

2020 ◽

Vol 15 (5) ◽

pp. 441-448 ◽

Cited By ~ 1

Author(s):

Wang Heng ◽

Mit Bhavsar ◽

Zhihua Han ◽

John H. Barker

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Cell Proliferation ◽

Stem Cell ◽

Electrical Stimulation ◽

Regenerative Medicine ◽

Cell Function ◽

Recent Interest ◽

Scaffold Materials ◽

Block Cell Proliferation

Recent interest in developing new regenerative medicine- and tissue engineering-based treatments has motivated researchers to develop strategies for manipulating stem cells to optimize outcomes in these potentially, game-changing treatments. Cells communicate with each other, and with their surrounding tissues and organs via electrochemical signals. These signals originate from ions passing back and forth through cell membranes and play a key role in regulating cell function during embryonic development, healing, and regeneration. To study the effects of electrical signals on cell function, investigators have exposed cells to exogenous electrical stimulation and have been able to increase, decrease and entirely block cell proliferation, differentiation, migration, alignment, and adherence to scaffold materials. In this review, we discuss research focused on the use of electrical stimulation to manipulate stem cell function with a focus on its incorporation in tissue engineering-based treatments.

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Stimuli-responsive supramolecules for bone tissue engineering

Biointerface Research in Applied Chemistry ◽

10.33263/briac102.122127 ◽

2020 ◽

Vol 10 (2) ◽

pp. 5122-5127

Keyword(s):

Tissue Engineering ◽

Ionic Strength ◽

Regenerative Medicine ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tissue Repair ◽

3D Bioprinting ◽

Stimuli Responsive ◽

Hydrogel Scaffolds ◽

Physical Stimuli

In the recent scenario Stimuli responsive supramolecules are used for bone tissue engineering. Stimuli responsive Supramolecules are responding towards the desired stimuli. This has a property to change their dynamics and undergo impulsive and continual assembly or disassembly processes under specific conditions. These supramolecules respond towards chemical and physical stimuli which include: pH, temperature, light, ionic strength, magnetic and electric field sensitive. Stimuli responsive supramolecules are used to various preparations such as hydrogels, scaffolds, hydrogel scaffolds, 3D bioprinting, 4D bioprinting, nanogels and microgels used for the bone tissue repair and regenerative medicine. Manuscript deals with various approaches used to prepare stimuli responsive supramolecules for bone engineering applications.

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