Identification of salt tolerance genes from the mangrove plant Bruguiera gymnorhiza using Agrobacterium functional screening

AbstractSalt stress is one of the abiotic stresses affecting crop growth and yield. The functional screening and mechanism investigation of the genes in response to salt stress are essential for the development of salt-tolerant crops. Here, we found that OXIDATIVE STRESS 2 (OXS2) was a salinity-induced gene, and the mutant oxs2-1 was hypersensitive to salt stress during seed germination and root elongation processes. In the absence of stress, OXS2 was predominantly localized in the cytoplasm; when the plants were treated with salt, OXS2 entered the nuclear. Further RNA-seq analysis and qPCR identification showed that, in the presence of salt stress, a large number of differentially expressed genes (DEGs) were activated, which contain BOXS2 motifs previously identified as the binding element for AtOXS2. Further ChIP analysis revealed that, under salt stress, OXS2 associated with CA1 and Araport11 directly through binding the BOXS2 containing fragments in the promoter regions. In conclusion, our results indicate that OXS2 is required for salt tolerance in Arabidopsis mainly through associating with the downstream CA1 and Araport11 directly.

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Overexpression of monodehydroascorbate reductase from a mangrove plant (AeMDHAR) confers salt tolerance on rice

Journal of Plant Physiology ◽

10.1016/j.jplph.2011.09.004 ◽

2012 ◽

Vol 169 (3) ◽

pp. 311-318 ◽

Cited By ~ 48

Author(s):

Shahanaz Sultana ◽

Choy-Yuen Khew ◽

Md. Mahbub Morshed ◽

Parameswari Namasivayam ◽

Suhaimi Napis ◽

...

Keyword(s):

Salt Tolerance ◽

Monodehydroascorbate Reductase ◽

Mangrove Plant

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Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

International Journal of Molecular Sciences ◽

10.3390/ijms19113446 ◽

2018 ◽

Vol 19 (11) ◽

pp. 3446 ◽

Cited By ~ 6

Author(s):

Mei Zhang ◽

Hui Zhang ◽

Jie-Xuan Zheng ◽

Hui Mo ◽

Kuai-Fei Xia ◽

...

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Cdna Library ◽

Molecular Mechanisms ◽

Full Length ◽

Cdna Clones ◽

Functional Screening ◽

Yeast Mutant ◽

Full Length Cdna ◽

Stress Related Genes

Ipomoea pes-caprae is a seashore halophytic plant and is therefore a good model for studying the molecular mechanisms underlying salt and stress tolerance in plant research. Here, we performed Full-length cDNA Over-eXpressor (FOX) gene hunting with a functional screening of a cDNA library using a salt-sensitive yeast mutant strain to isolate the salt-stress-related genes of I. pes-caprae (IpSR genes). The library was screened for genes that complemented the salt defect of yeast mutant AXT3 and could grow in the presence of 75 mM NaCl. We obtained 38 candidate salt-stress-related full-length cDNA clones from the I. pes-caprae cDNA library. The genes are predicted to encode proteins involved in water deficit, reactive oxygen species (ROS) scavenging, cellular vesicle trafficking, metabolic enzymes, and signal transduction factors. When combined with the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses, several potential functional salt-tolerance-related genes were emphasized. This approach provides a rapid assay system for the large-scale screening of I. pes-caprae genes involved in the salt stress response and supports the identification of genes responsible for the molecular mechanisms of salt tolerance.

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Proteomic Analysis of Salt-Responsive Proteins in the Mangrove Plant, Bruguiera gymnorhiza

Plant and Cell Physiology ◽

10.1093/pcp/pcp002 ◽

2009 ◽

Vol 50 (3) ◽

pp. 439-446 ◽

Cited By ~ 52

Author(s):

Y. Tada ◽

T. Kashimura

Keyword(s):

Proteomic Analysis ◽

Mangrove Plant ◽

Bruguiera Gymnorhiza

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Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

International Journal of Molecular Sciences ◽

10.3390/ijms21010118 ◽

2019 ◽

Vol 21 (1) ◽

pp. 118 ◽

Cited By ~ 2

Author(s):

Yi-Ling Liu ◽

Zhi-Jun Shen ◽

Martin Simon ◽

Huan Li ◽

Dong-Na Ma ◽

...

Keyword(s):

Salt Tolerance ◽

Proteomic Analysis ◽

Carbon Fixation ◽

Triosephosphate Isomerase ◽

High Salinity ◽

Chlorophyll Biosynthesis ◽

Primary Metabolism ◽

Kandelia Obovata ◽

Mangrove Species ◽

Mangrove Plant

As a dominant mangrove species, Kandelia obovata is distributed in an intertidal marsh with an active H2S release. Whether H2S participates in the salt tolerance of mangrove plants is still ambiguous, although increasing evidence has demonstrated that H2S functions in plant responses to multiple abiotic stresses. In this study, NaHS was used as an H2S donor to investigate the regulatory mechanism of H2S on the salt tolerance of K. obovata seedlings by using a combined physiological and proteomic analysis. The results showed that the reduction in photosynthesis (Pn) caused by 400 mM of NaCl was recovered by the addition of NaHS (200 μM). Furthermore, the application of H2S enhanced the quantum efficiency of photosystem II (PSII) and the membrane lipid stability, implying that H2S is beneficial to the survival of K. obovata seedlings under high salinity. We further identified 37 differentially expressed proteins by proteomic approaches under salinity and NaHS treatments. Among them, the proteins that are related to photosynthesis, primary metabolism, stress response and hormone biosynthesis were primarily enriched. The physiological and proteomic results highlighted that exogenous H2S up-regulated photosynthesis and energy metabolism to help K. obovata to cope with high salinity. Specifically, H2S increased photosynthetic electron transfer, chlorophyll biosynthesis and carbon fixation in K. obovata leaves under salt stress. Furthermore, the abundances of other proteins related to the metabolic pathway, such as antioxidation (ascorbic acid peroxidase (APX), copper/zinc superoxide dismutase (CSD2), and pancreatic and duodenal homeobox 1 (PDX1)), protein synthesis (heat-shock protein (HSP), chaperonin family protein (Cpn) 20), nitrogen metabolism (glutamine synthetase 1 and 2 (GS2), GS1:1), glycolysis (phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI)), and the ascorbate–glutathione (AsA–GSH) cycle were increased by H2S under high salinity. These findings provide new insights into the roles of H2S in the adaptations of the K. obovata mangrove plant to high salinity environments.

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Transcriptome Analysis of Salt Stress in Hibiscus hamabo Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing

International Journal of Molecular Sciences ◽

10.3390/ijms23010138 ◽

2021 ◽

Vol 23 (1) ◽

pp. 138

Author(s):

Longjie Ni ◽

Zhiquan Wang ◽

Xiangdong Liu ◽

Shuting Wu ◽

Jianfeng Hua ◽

...

Keyword(s):

Signal Transduction ◽

Salt Stress ◽

Salt Tolerance ◽

Differentially Expressed Genes ◽

Single Molecule ◽

Treatment Time ◽

Full Length ◽

Differentially Expressed ◽

Illumina Platform ◽

Mangrove Plant

Hibiscus hamabo Sieb. et Zucc is an important semi-mangrove plant with great morphological features and strong salt resistance. In this study, by combining single molecule real time and next-generation sequencing technologies, we explored the transcriptomic changes in the roots of salt stressed H. hamabo. A total of 94,562 unigenes were obtained by clustering the same isoforms using the PacBio RSII platform, and 2269 differentially expressed genes were obtained under salt stress using the Illumina platform. There were 519 differentially expressed genes co-expressed at each treatment time point under salt stress, and these genes were found to be enriched in ion signal transduction and plant hormone signal transduction. We used Arabidopsis thaliana (L.) Heynh. transformation to confirm the function of the HhWRKY79 gene and discovered that overexpression enhanced salt tolerance. The full-length transcripts generated in this study provide a full characterization of the transcriptome of H. hamabo and may be useful in mining new salt stress-related genes specific to this species, while facilitating the understanding of the salt tolerance mechanisms.

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