Microbial enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase: An elixir for plant under stress

2021 ◽  
pp. 101664
Author(s):  
Chaitanya Kumar Jha ◽  
Priyanka Sharma ◽  
Arpit Shukla ◽  
Paritosh Parmar ◽  
Rohit Patel ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Acc Deaminase ◽  
Microbial Enzyme

scholarly journals Draft Genome Sequence of Endophytic BacteriumEnterobacter asburiaePDA134, Isolated from Date Palm (Phoenix dactyliferaL.) Roots

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Mahmoud W. Yaish
Keyword(s):  
Acetic Acid ◽  
Carboxylic Acid ◽  
Salinity Stress ◽  
Genome Sequence ◽  
Bacterial Strain ◽  
Date Palm ◽  
Draft Genome ◽  
Acc Deaminase ◽  
Enterobacter Asburiae ◽  
Indole 3 Acetic Acid

In this report, a draft of theEnterobacter asburiaestrain PDA134 genome was sequenced. This bacterial strain was isolated from the root tissue of a date palm, where it has the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and indole-3-acetic acid (IAA) under salinity stress.

scholarly journals Molecular Mechanisms of the 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Deaminase Producing Trichoderma asperellum MAP1 in Enhancing Wheat Tolerance to Waterlogging Stress

2021 ◽  
Vol 11 ◽  
Author(s):  
Mamoona Rauf ◽  
Muhammad Awais ◽  
Aziz Ud-Din ◽  
Kazim Ali ◽  
Humaira Gul ◽  
...  
Keyword(s):  
Gene Expression ◽  
Carboxylic Acid ◽  
Stomatal Conductance ◽  
Molecular Mechanisms ◽  
Acc Deaminase ◽  
Antioxidant Enzyme Activities ◽  
Trichoderma Asperellum ◽  
Wheat Growth ◽  
Wheat Seedlings ◽  
Waterlogging Stress

Waterlogging stress (WS) induces ethylene (ET) and polyamine (spermine, putrescine, and spermidine) production in plants, but their reprogramming is a decisive element for determining the fate of the plant upon waterlogging-induced stress. WS can be challenged by exploring symbiotic microbes that improve the plant’s ability to grow better and resist WS. The present study deals with identification and application of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing fungal endophyte Trichoderma asperellum (strain MAP1), isolated from the roots of Canna indica L., on wheat growth under WS. MAP1 positively affected wheat growth by secreting phytohormones/secondary metabolites, strengthening the plant’s antioxidant system and influencing the physiology through polyamine production and modulating gene expression. MAP1 inoculation promoted yield in comparison to non-endophyte inoculated waterlogged seedlings. Exogenously applied ethephon (ET synthesis inducer) and 1-aminocyclopropane carboxylic acid (ACC; ET precursor) showed a reduction in growth, compared to MAP1-inoculated waterlogged seedlings, while amino-oxyacetic acid (AOA; ET inhibitor) application reversed the negative effect imposed by ET and ACC, upon waterlogging treatment. A significant reduction in plant growth rate, chlorophyll content, and stomatal conductance was noticed, while H2O2, MDA production, and electrolyte leakage were increased in non-inoculated waterlogged seedlings. Moreover, in comparison to non-inoculated waterlogged wheat seedlings, MAP1-inoculated waterlogged wheat exhibited antioxidant–enzyme activities. In agreement with the physiological results, genes associated with the free polyamine (PA) biosynthesis were highly induced and PA content was abundant in MAP1-inoculated seedlings. Furthermore, ET biosynthesis/signaling gene expression was reduced upon MAP1 inoculation under WS. Briefly, MAP1 mitigated the adverse effect of WS in wheat, by reprogramming the PAs and ET biosynthesis, which leads to optimal stomatal conductance, increased photosynthesis, and membrane stability as well as reduced ET-induced leaf senescence.

Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings

2001 ◽  
Vol 47 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Donna M Penrose ◽  
Barbara A Moffatt ◽  
Bernard R Glick
Keyword(s):  
Carboxylic Acid ◽  
Plant Growth ◽  
Root Elongation ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Psychrophilic Bacterium ◽  
Ethylene Inhibitor ◽  
Seedling Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ*Tag Amino Acid Analysis Method(tm) was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.

scholarly journals Disruption and Overexpression of the Gene Encoding ACC (1-Aminocyclopropane-1-Carboxylic Acid) Deaminase in Soil-Borne Fungal Pathogen Verticillium dahliae Revealed the Role of ACC as a Potential Regulator of Virulence and Plant Defense

2019 ◽  
Vol 32 (6) ◽  
pp. 639-653 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
lakovos S. Pantelides ◽  
Aliki K. Tzima ◽  
Seogchan Kang ◽  
Epaminondas J. Paplomatas ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Plant Defense ◽  
Verticillium Dahliae ◽  
Fungal Pathogen ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Gene Encoding ◽  
Pathogen Virulence ◽  
Plant Growth Promoting

It has been suggested that some microorganisms, including plant growth–promoting rhizobacteria, manipulate the level of ethylene in plants by degrading 1-aminocyclopropane-1-carboxylic acid (ACC), an ethylene precursor, into α-ketobutyrate and ammonia, using ACC deaminase (ACCd). Here, we investigated whether ACCd of Verticillium dahliae, a soil-borne fungal pathogen of many important crops, is involved in causing vascular wilt disease. Overexpression of the V. dahliae gene encoding this enzyme, labeled as ACCd, significantly increased virulence in both tomato and eggplant, while disruption of ACCd reduced virulence. Both types of mutant produced more ethylene than a wild-type (70V-WT) strain, although they significantly differed in ACC content. Overexpression strains lowered ACC levels in the roots of infected plants, while the amount of ACC in the roots of plants infected with deletion mutants increased. To test the hypothesis that ACC acts as a signal for controlling defense, roots of WT and Never-ripe (Nr) tomato plants were treated with ACC before V. dahliae inoculation. Plants pretreated with ACC displayed less severe symptoms than untreated controls. Collectively, our results suggest a novel role of ACC as a regulator of both plant defense and pathogen virulence.

Effect of transferring 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase genes intoPseudomonas fluorescensstrain CHA0 and itsgacA derivative CHA96 on their growth-promoting and disease-suppressive capacities

2000 ◽  
Vol 46 (10) ◽  
pp. 898-907 ◽  
Author(s):  
Chunxia Wang ◽  
Edouard Knill ◽  
Bernard R Glick ◽  
Geneviève Défago
Keyword(s):  
Carboxylic Acid ◽  
Root Length ◽  
Regulatory Region ◽  
Acc Deaminase ◽  
Soft Rot ◽  
Plant Diseases ◽  
Potato Tubers ◽  
Experimental Conditions ◽  
Growth Promoting

Pseudomonas fluorescens strain CHA0, a root colonizing bacterium, has a broad spectrum of biocontrol activity against plant diseases. However, strain CHA0 is unable to utilize 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of plant ethylene, as a sole source of nitrogen. This suggests that CHA0 does not contain the enzyme ACC deaminase, which cleaves ACC to ammonia and α-ketobutyrate, and was previously shown to promote root elongation of plant seedlings treated with bacteria containing this enzyme. An ACC deaminase gene, together with its regulatory region, was transferred into P. fluorescens strains CHA0 and CHA96, a global regulatory gacA mutant of CHA0. ACC deaminase activity was expressed in both CHA0 and CHA96. Transformed strains with ACC deaminase activity increased root length of canola plants under gnotobiotic conditions, whereas strains without this activity had no effect. Introduction of ACC deaminase genes into strain CHA0 improved its ability to protect cucumber against Pythium damping-off, and potato tubers against Erwinia soft rot in small hermetically sealed containers. In contrast, ACC deaminase activity had no significant effect on the ability of CHA0 to protect tomato against Fusarium crown and root rot, and potato tubers against soft rot in large hermetically sealed containers. These results suggest that (i) ACC deaminase activity may have lowered the level of plant ethylene thereby increasing root length; (ii) the role of stress-generated plant ethylene in susceptibility or resistance depends on the host-pathogen system, and on the experimental conditions used; and (iii) the constructed strains could be developed as biosensors for the role of ethylene in plant diseases.Key words: biocontrol, plant growth-promoting rhizobacteria, ACC deaminase, ethylene biosensors.

scholarly journals Overexpression of 1-Aminocyclopropane-1-Carboxylic Acid Deaminase (acdS) Gene in Petunia hybrida Improves Tolerance to Abiotic Stresses

2021 ◽  
Vol 12 ◽  
Author(s):  
Aung Htay Naing ◽  
Hui Yeong Jeong ◽  
Sung Keun Jung ◽  
Chang Kil Kim
Keyword(s):  
Carboxylic Acid ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Ethylene Production ◽  
Abiotic Stresses ◽  
Petunia Hybrida ◽  
Ornamental Plants ◽  
Acc Oxidase ◽  
Acc Deaminase ◽  
Ethylene Biosynthesis

Abiotic stress induces the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in plants, which consequently enhances ethylene production and inhibits plant growth. The bacterial ACC deaminase enzyme encoded by the acdS gene reduces stress-induced ethylene production and improves plant growth in response to stress. In this study, overexpression of acdS in Petunia hybrida (‘Mirage Rose’) significantly reduced expression of the ethylene biosynthesis gene ACC oxidase 1 (ACO1) and ethylene production relative to those in wild type (WT) under various abiotic stresses (cold, drought, and salt). The higher reduction of stress-induced ethylene in the transgenic plants, which was due to the overexpression of acdS, led to a greater tolerance to the stresses compared to that in the WT plants. The greater stress tolerances were proven based on better plant growth and physiological performance, which were linked to stress tolerance. Moreover, expression analysis of the genes involved in stress tolerance also supported the increased tolerance of transgenics relative to that with the WT. These results suggest the possibility that acdS is overexpressed in ornamental plants, particularly in bedding plants normally growing outside the environment, to overcome the deleterious effect of ethylene on plant growth under different abiotic stresses. The development of stress-tolerant plants will be helpful to advance the floricultural industry.

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