Synthesis and antimicrobial potential of spirooxindolopyrrolidine tethered oxindole heterocyclic hybrid against multidrug resistant microbial pathogens

2022 ◽  
Author(s):  
Shatha Ibrahim Alaqeel ◽  
Natarajan Arumugam ◽  
Abdulrahman I. Almansour ◽  
Raju Suresh Kumar ◽  
Karuppiah Ponmurugan ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Microbial Pathogens ◽  
Antimicrobial Potential

Green and red Brazilian propolis: antimicrobial potential and anti‐virulence against ATCC and clinically isolated multidrug‐resistant bacteria

2021 ◽  
Author(s):  
Thayná de Souza Silva ◽  
Júlia M. B. Silva ◽  
Gláucia H. Braun ◽  
Jennyfer A. A. Mejia ◽  
Gari V. C. Ccapatinta ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Antimicrobial Potential ◽  
Brazilian Propolis

In vitro antimicrobial potential of Terminalia chebula fruit extracts against multidrug–resistant uropathogens

2012 ◽  
Vol 2 (3) ◽  
pp. S1883-S1887 ◽  
Author(s):  
Anwesa Bag ◽  
Subir Kumar Bhattacharyya ◽  
Nishith Kumar Pal ◽  
Rabi Ranjan Chattopadhyay
Keyword(s):  
Multidrug Resistant ◽  
Terminalia Chebula ◽  
Fruit Extracts ◽  
Antimicrobial Potential

scholarly journals Alkylaminoquinolines inhibit the bacterial antibiotic efflux pump in multidrug-resistant clinical isolates

2003 ◽  
Vol 376 (3) ◽  
pp. 801-805 ◽  
Author(s):  
Monique MALLÉA ◽  
Abdallah MAHAMOUD ◽  
Jacqueline CHEVALIER ◽  
Sandrine ALIBERT-FRANCO ◽  
Pierre BROUANT ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Efflux Pump ◽  
Efflux Pumps ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Microbial Pathogens ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
High Level ◽  
Antibiotic Efflux

Over the last decade, MDR (multidrug resistance) has increased worldwide in microbial pathogens by efflux mechanisms, leading to treatment failures in human infections. Several Gram-negative bacteria efflux pumps have been described. These proteinaceous channels are capable of expelling structurally different drugs across the envelope and conferring antibiotic resistance in various bacterial pathogens. Combating antibiotic resistance is an urgency and the blocking of efflux pumps is an attractive response to the emergence of MDR phenotypes in infectious bacteria. In the present study, various alkylaminoquinolines were tested as potential inhibitors of drug transporters. We showed that alkylaminoquinolines are capable of restoring susceptibilities to structurally unrelated antibiotics in clinical isolates of MDR Gram-negative bacteria. Antibiotic efflux studies indicated that 7-nitro-8-methyl-4-[2´-(piperidino)ethyl]aminoquinoline acts as an inhibitor of the AcrAB–TolC efflux pump and restores a high level of intracellular drug concentration. Inhibitory activity of this alkylaminoquinoline is observed on clinical isolates showing different resistance phenotypes.

scholarly journals Antibacterial potential of indigenous plant extracts against multidrug-resistant bacterial strains isolated from New Delhi region

2021 ◽  
Vol 14 (2) ◽  
pp. 185-196
Author(s):  
Jatin Chadha ◽  
Manish Gupta ◽  
Nishtha Nagpal ◽  
Madhav Sharma ◽  
Tarun Adarsh ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Bacterial Infections ◽  
Intervention Strategy ◽  
Multidrug Resistant ◽  
Microbial Pathogens ◽  
Ocimum Sanctum ◽  
Human Pathogens ◽  
Bacterial Strains ◽  
Antibacterial Potential

The extensive use of antibiotics to treat bacterial infections has led to the widespread emergence of multidrug-resistant (MDR) pathogens, becoming increasingly difficult to treat with currently available antibacterial agents. The present study is based on prospecting the ethnomedicinal potential of Indian plant varieties for the treatment of MDR bacteria. Plants produce an array of diverse pharmacological compounds in defence against microbial pathogens which may be employed as a novel intervention strategy to combat MDR human pathogens. In the present study, the antimicrobial activity of extracts of four common Indian plants: Azadirachta indica (Neem), Murraya koenigii (Kadipatta), Phyllanthus emblica (Amla), and Ocimum sanctum (Tulsi) prepared in four solvents, water, methanol, ethanol, and chloroform was tested against nine MDR bacterial isolates. Kirby-Bauer well diffusion assays were adopted to assess the antimicrobial activity of plant extracts against the MDR strains. The potency of plant extracts was examined by determining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). All MDR isolates including Staphylococcus haemolyticus, Bacillus subtilis, B. thuringiensis, B. cereus, Enterobacter xiangfangensis, Klebsiella pneumoniae, and Pseudomonas aeruginosa were significantly inhibited by the plant extracts. Test extracts showed promising antibacterial potential against MDR P. aeruginosa and Bacillus sp. with low MIC values ranging between 0.02-1.56 mg/ml, while most plant extracts exhibited either moderate MBC values or bacteriostatic effects. To the best of our knowledge, this is the first study that demonstrates the potential use of endemic A. indica, M. koenigii, P. emblica, and O. sanctum as therapeutic agents against circulating MDR human pathogens in the national capital.

scholarly journals Potent, specific MEPicides for treatment of zoonotic staphylococci

2019 ◽  
Author(s):  
Rachel L. Edwards ◽  
Isabel Heueck ◽  
Soon Goo Lee ◽  
Ishaan T. Shah ◽  
Andrew J. Jezewski ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Opportunistic Infections ◽  
Multidrug Resistant ◽  
Isoprenoid Biosynthesis ◽  
Forward Genetics ◽  
Microbial Pathogens ◽  
Cellular Transport ◽  
Human Welfare ◽  
Zoonotic Infections ◽  
Tract Infections

AbstractCoagulase-positive staphylococci, which frequently colonize the mucosal surfaces of animals, also cause a spectrum of opportunistic infections including skin and soft tissue infections, urinary tract infections, pneumonia, and bacteremia. However, recent advances in bacterial identification have revealed that these common veterinary pathogens are in fact, zoonoses that cause serious infections in human patients. The global spread of multidrug-resistant zoonotic staphylococci, in particular the emergence of methicillin-resistant organisms, is now a serious threat to both animal and human welfare. Accordingly, new therapeutic targets that can be exploited to combat staphylococcal infections are urgently needed. Enzymes of the methylerythritol phosphate pathway (MEP) of isoprenoid biosynthesis represent potential targets for treating zoonotic staphylococci. Here we demonstrate that fosmidomycin (FSM) inhibits the first step of the isoprenoid biosynthetic pathway catalyzed by deoxyxylulose phosphate reductoisomerase (DXR) in staphylococci. In addition, we have both enzymatically and structurally determined the mechanism by which FSM elicits its effect. Using a forward genetic screen, the glycerol-3-phosphate transporter GlpT that facilitates FSM uptake was identified in two zoonotic staphylococci, Staphylococcus schleiferi and Staphylococcus pseudintermedius. A series of lipophilic ester prodrugs (termed MEPicides) structurally related to FSM were synthesized, and data indicate that the presence of the prodrug moiety not only substantially increased potency of the inhibitors against staphylococci, but also bypassed the need for GlpT-mediated cellular transport. Collectively, our data indicate that the prodrug MEPicides selectively and robustly inhibit DXR in zoonotic staphylococci, and further, DXR represents a promising, druggable target for future development.Author SummaryThe proliferation of microbial pathogens resistant to the current pool of antibiotics is a major threat to public health. Drug resistance is pervasive in staphylococci, including several species that can cause serious zoonotic infections in humans. Thus, new antimicrobial agents are urgently need to combat these life-threatening, resistant infections. Here we establish the MEP pathway as a promising new target against zoonotic staphylococci. We determine that fosmidomycin (FSM) selectively targets the isoprenoid biosynthesis pathway in zoonotic staphylococci, and use forward genetics to identify the transporter that facilitates phosphonate antibiotic uptake. Employing this knowledge, we synthesized a series of potent antibacterial prodrugs that circumvent the transporter. Together, these novel prodrug inhibitors represent promising leads for further drug development against zoonotic staphylococci.

scholarly journals Antimicrobial Potential of Sonneratia alba and Sonneratia caseolaris against Shrimp Pathogens

2021 ◽  
pp. 7-14
Author(s):  
Dian Yuni Pratiwi
Keyword(s):  
Bioactive Compounds ◽  
Antimicrobial Agents ◽  
Shrimp Farming ◽  
Microbial Pathogens ◽  
Economic Losses ◽  
Negative Effects ◽  
Antimicrobial Potential ◽  
Sonneratia Caseolaris ◽  
Sonneratia Alba

Disease is one of the obstacles in shrimp farming. Many countries have experienced economic losses due to disease in shrimp caused by microbes. Many strategies are being used to overcome the problem such as antibiotics, formalin, probiotics, prebiotics, synbiotics, and others. However, the use antibiotics in long term can cause negative effects. So that, the development of potential new natural compounds is required to overcome this problem. This review article aims to explain the nutritional content, bioactive compounds, antimicrobial potential, and the effect of S. alba and S. caseolaris on shrimp survival. Sonneratia alba and Sonneratia caseolaris are plants that have many bioactive compounds such as alkaloids, flavonoids, terpenoids, and phenolics. They have also been shown to inhibit the growth of bacteria such as Vibrio harveyi, Escherichia coli, Staphylococcus aureus, Saprolegnia sp., and others. Application of S. alba and S. caseolaris can also increase the survival rate of infected shrimps. S. alba and S. caseolaris have the potential to be used as antimicrobial agents and can be used to protect shrimp from microbial pathogens.

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