Antimicrobial potential of bioactive metabolites, Silver nanoparticle from Bacillus spp. and some antibiotics against Multidrug Resistant Salmonella spp.

ABSTRACT: This study aimed to evaluate the microbiological quality and the transmission of multidrug-resistant bacteria in different spices sold in town fairs (local food markets) in the municipalities of Recôncavo Baiano. Samples of black pepper, oregano, and cinnamon were collected over a period of six months and investigated for coliforms at 45 °C, Staphylococcus spp., Staphylococcus aureus, Bacillus spp., Bacillus cereus, Escherichia coli and Salmonella spp. The contamination in the black pepper samples (log 4.66 CFU g-1) was higher (P>0.05), than those of cinnamon (log 2.55 CFU g-1) and oregano (log 2.49 CFU g-1), particularly for B. cereus. E. coli (89%) and Salmonella spp. (67%) were isolated only from black pepper. B. cereus and S. aureus showed greater resistance to β-lactams (penicillin, oxacillin, and cefepime), with approximately 40% of the strains with a multiple antimicrobial resistance (MAR) index of 0.33 (i.e., resistant to three antimicrobials). E. coli was more resistant to ampicillin and Salmonella spp. to nalidixic acid, ampicillin, and ceftriaxone. Salmonella spp. had a MAR index ranging from 0.16 to 0.91 (i.e, resistant to up to 11 antimicrobials), and E. coli to up to 0.58 (i.e., resistant to 7 antimicrobials). In conclusion, the spices sold in the town fairs of Recôncavo Baiano are of low microbiological quality, with the presence of pathogens, of which some display high resistance to antimicrobials that are commonly used for treating foodborne illnesses.

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Influence of tannery wastes and the surrounding environment of tannery industries on microbial growth and proliferation

Stamford Journal of Microbiology ◽

10.3329/sjm.v7i1.40065 ◽

2017 ◽

Vol 7 (1) ◽

pp. 14-18

Author(s):

Nayan Chandra Das ◽

Farzana Hossaini ◽

Saurab Kishore Munshi

Keyword(s):

Pathogenic Bacteria ◽

Multidrug Resistant ◽

Salmonella Spp ◽

Tannery Waste ◽

Surrounding Environment ◽

Bacillus Spp ◽

Tannery Wastes ◽

Bacteria And Fungi ◽

Staphylococcus Spp ◽

Klebsiella Spp

The present study was carried out to assess the degree of microbiological proliferation in tannery wastes and the surrounding environment of the tannery industries. In this regard, a total of 8 tannery waste (n=4) and environmental (n=4) samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 108 cfu/g or ml. An extended numbers of pathogenic bacteria were recovered. Among the pathogenic bacteria, Staphylococcus spp. was predominant. Most of the samples exhibited the presence of Pseudomonas spp. Salmonella spp. and fecal coliform were found each in one sample. Bacillus spp., Escherichia coli, Klebsiella spp. and Vibrio spp. were found in few samples. The average load of the pathogens was 104 cfu/g or ml. All the pathogenic isolates were found to be multidrug resistant. Higher resistance was found against penicillin and streptomycin. Tannery waste after lather treatment sample showed antibacterial activity against all the pathogens tested. Overall, presence of pathogenic microorganisms with multidrug resistance traits may pose serious public health threats. Stamford Journal of Microbiology, Vol.7(1) 2017: 14-18

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Antimicrobial Potential of Bioactive Metabolites and Silver Nanoparticles from Bacillus spp. and of Some Antibiotics Against Multidrug Resistant Salmonella spp.

Turkish Journal of Pharmaceutical Sciences ◽

10.4274/tjps.galenos.2019.46548 ◽

2020 ◽

Vol 17 (5) ◽

pp. 511-522

Author(s):

Bukola Christianah Adebayo-Tayo ◽

Oluwadara EKUNDAYO-OBABA ◽

Olutayo Israel FALODUN

Keyword(s):

Silver Nanoparticles ◽

Multidrug Resistant ◽

Bioactive Metabolites ◽

Antimicrobial Potential

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Microbiological investigation and determination of the antimicrobial potential of cow dung samples

Stamford Journal of Microbiology ◽

10.3329/sjm.v8i1.42437 ◽

2019 ◽

Vol 8 (1) ◽

pp. 34-37 ◽

Cited By ~ 1

Author(s):

Saurab Kishore Munshi ◽

Juel Roy ◽

Rashed Noor

Keyword(s):

Antimicrobial Activity ◽

Pathogenic Bacteria ◽

Cow Dung ◽

Agricultural Field ◽

Salmonella Spp ◽

Antimicrobial Potential ◽

Bacillus Spp ◽

Bacteria And Fungi ◽

Staphylococcus Spp

Cow dung is being used in agriculture as well for the household and religious purposes from the ancient time. Cow dung is known to possess antimicrobial activity and contains a wide variety of microorganisms with variable properties. Therefore, the present study was carried out to assess the microbial diversity including pathogenic ones of the cow dung samples as well as to determine the antimicrobial traits of the samples. In this regard, a total of 8 fresh cow dung samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 108 and 107 cfu/g, respectively. An extended number of pathogenic bacteria were recovered. Among the pathogenic bacteria, Staphylococcus spp. and Bacillus spp. were predominantly found in every sample. The presence of Pseudomonas spp. Escherichia coli, Klebsiella spp. and fecal coliform were exhibited in most of the samples. Salmonella spp. and Vibrio spp. were found in 3 and 5 samples, respectively. The average load of the pathogens was 104 cfu/g. All the samples showed substantial degree of antimicrobial activity against all the pathogens tested. Samples 1, 2, 3 and 4 were effective in inhibiting the growth of all the tested pathogens. The findings of the present study revealed the need for safe application of cow dung in the agricultural field and of further investigation for the antimicrobial potential of the diversified microflora of cow dung offering agricultural, environmental and medical applications. Stamford Journal of Microbiology, Vol.8(1) 2018: 34-37

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Occurrence of drug resistant bacteria in household waste samples

Stamford Journal of Microbiology ◽

10.3329/sjm.v9i1.45652 ◽

2020 ◽

Vol 9 (1) ◽

pp. 15-18

Author(s):

Aklima Akter ◽

Sadia Ahmed ◽

Tamanna Islam ◽

Saurab Kishore Munshi

Keyword(s):

Bacterial Load ◽

Liquid Waste ◽

Drug Susceptibility ◽

Multidrug Resistant ◽

Household Waste ◽

Resistant Bacteria ◽

Drug Resistant ◽

Salmonella Spp ◽

Drug Resistant Bacteria ◽

Bacillus Spp

Current study was carried out to investigate the presence of drug resistant bacterial isolates in the biodegradable household waste samples. In this respect, a total of six domestic waste samples including three kitchen waste samples and one sample each of home dust, dustbin waste and liquid waste were collected. Samples were analyzed for quantification of bacterial load and along with their drug susceptibility pattern. Huge array of total viable bacteria was present in all the samples (in average of 108 cfu/g or ml). Among the specific bacteria, Bacillus spp. was predominant and Vibrio spp. was found in almost all samples except liquid waste. Presence of Staphylococcus spp., E. coli, Klebsiella spp., Pseudomonas spp., Salmonella spp. and fecal coliform were evident in some samples. All isolates were found to be multidrug resistant. Notably, 100% resistance was documented against cefuroxime and amoxicillin. All the isolates showed sensitivity against meropenem, amikacin and ceftriaxone. Presence of drug resistant bacteria in household waste samples in present study critically raises the requirement for proper management and disposal of the accumulated domestic wastes by the municipal and government authorities. Stamford Journal of Microbiology, Vol.9(1) 2019: 15-18

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Multidrug Resistant Bacteria in the Respiratory Tract of Apparently Healthy Quails

Microbes and Health ◽

10.3329/mh.v1i2.14088 ◽

2013 ◽

Vol 1 (2) ◽

pp. 46-49 ◽

Cited By ~ 1

Author(s):

Shobnom Sultana ◽

Md Ariful Islam ◽

Mst. Minara Khatun ◽

Shamima Nasrin

Keyword(s):

Respiratory Tract ◽

Nalidixic Acid ◽

Antibiotic Sensitivity ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Salmonella Spp ◽

Japanese Quails ◽

Bacillus Spp ◽

Staphylococcus Spp ◽

Apparently Healthy

The study was undertaken for isolation, identification and in vitro antibiotic sensitivity assay of bacteria present in the respiratory tract of apparently healthy Japanese quails. A total of 50 samples comprised of tracheal swabs (n = 18), tracheal washings (n = 8), air sacs (n = 8), lungs (n = 8) and exudates of infraorbital sinuses (n = 8) were aseptically collected from 26 apparently healthy Japanese quails. The samples were inoculated onto a variety of media for isolation of bacteria. Identification of bacteria was performed by colony morphology, Grams staining and biochemical tests. In total, 25 (50%), 9 (18%), 22 (44%), 20 (40%) and 24 (48%) isolates were identified as Escherichia coli, Salmonella spp., Pasteurella spp., Bacillus spp. and Staphylococcus spp. respectively. Antibiotic sensitivity tests of one randomly selected isolate from each genus of bacteria were performed against 10 common antibiotics. E. coli showed resistance to four antibiotics (amoxicillin, gentamycin, nalidixic acid and tetracycline); Salmonella spp. showed resistance to seven antibiotics (amoxicillin, ampicillin, erythromycin, gentamycin, kanamycin, nalidixic acid and tetracycline); Pasteurella spp. to three antibiotics (erythromycin, sulphamethoxazole and tetracycline); Bacillus spp. to chloramphenicol and nalidixic acid and Staphylococcus spp. to amoxicillin and ampicillin. All the isolates were susceptible to ciprofloxacin. Data of this study indicate that multidrug resistant bacteria are present in the respiratory tract of clinically healthy quails. This is the first report of isolation, identification and antibiogram profile of bacteria present in the respiratory tract of quails in Bangladesh. DOI: http://dx.doi.org/10.3329/mh.v1i2.14088 Microbes and Health, 2012 1(2): 46-49

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Antimicrobial Resistance and Presence of Class 1 Integrons Among Different Serotypes of Salmonella spp. Recovered From Children with Diarrhea in Tehran, Iran

Infectious Disorders - Drug Targets ◽

10.2174/1871526519666190130171020 ◽

2020 ◽

Vol 20 (2) ◽

pp. 160-166

Author(s):

Seyedeh Hanieh Eshaghi Zadeh ◽

Hossein Fahimi ◽

Fatemeh Fardsanei ◽

Mohammad Mehdi Soltan Dallal

Keyword(s):

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Multidrug Resistant ◽

Salmonella Spp ◽

Class 1 Integron ◽

Class 1 Integrons ◽

Food Borne ◽

Class 1 ◽

Resistance Patterns ◽

C 30

Background: Salmonellosis is a major food-borne disease worldwide. The increasing prevalence of antimicrobial resistance among food-borne pathogens such as Salmonella spp. is concerning. Objective: The main objective of this study is to identify class 1 integron genes and to determine antibiotic resistance patterns among Salmonella isolates from children with diarrhea. Methods: A total of 30 Salmonella isolates were recovered from children with diarrhea. The isolates were characterized for antimicrobial susceptibility and screened for the presence of class 1 integron genes (i.e. intI1, sulI1, and qacEΔ1). Results: The most prevalent serotype was Enteritidis 36.7%, followed by Paratyphi C (30%), and Typhimurium (16.7%). The highest rates of antibiotic resistance were obtained for nalidixic acid (53.3%), followed by streptomycin (40%), and tetracycline (36.7%). Regarding class 1 integrons, 36.7%, 26.7%, and 33.3% of the isolates carried intI1, SulI, and qacEΔ1, respectively, most of which (81.8%) were multidrug-resistant (MDR). Statistical analysis revealed that the presence of class 1 integron was significantly associated with resistance to streptomycin and tetracycline (p = 0.042). However, there was no association between class 1 integron and other antibiotics used in this study (p > 0.05). Conclusion: The high frequency of integron class 1 gene in MDR Salmonella strains indicates that these mobile genetic elements are versatile among different Salmonella serotypes, and associated with reduced susceptibility to many antimicrobials.

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Antimicrobial Activities of Marine Sponge-Associated Bacteria

Microorganisms ◽

10.3390/microorganisms9010171 ◽

2021 ◽

Vol 9 (1) ◽

pp. 171

Author(s):

Yitayal S. Anteneh ◽

Qi Yang ◽

Melissa H. Brown ◽

Christopher M. M. Franco

Keyword(s):

Pathogenic Bacteria ◽

South Australia ◽

Multidrug Resistant ◽

Antimicrobial Activities ◽

Marine Sponges ◽

Bioactive Metabolites ◽

Human Pathogens ◽

Sponge Associated Bacteria ◽

Microbial Symbionts ◽

Bacteria And Fungi

The misuse and overuse of antibiotics have led to the emergence of multidrug-resistant microorganisms, which decreases the chance of treating those infected with existing antibiotics. This resistance calls for the search of new antimicrobials from prolific producers of novel natural products including marine sponges. Many of the novel active compounds reported from sponges have originated from their microbial symbionts. Therefore, this study aims to screen for bioactive metabolites from bacteria isolated from sponges. Twelve sponge samples were collected from South Australian marine environments and grown on seven isolation media under four incubation conditions; a total of 1234 bacterial isolates were obtained. Of these, 169 bacteria were tested in media optimized for production of antimicrobial metabolites and screened against eleven human pathogens. Seventy bacteria were found to be active against at least one test bacterial or fungal pathogen, while 37% of the tested bacteria showed activity against Staphylococcus aureus including methicillin-resistant strains and antifungal activity was produced by 21% the isolates. A potential novel active compound was purified possessing inhibitory activity against S. aureus. Using 16S rRNA, the strain was identified as Streptomyces sp. Our study highlights that the marine sponges of South Australia are a rich source of abundant and diverse bacteria producing metabolites with antimicrobial activities against human pathogenic bacteria and fungi.

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The Use of Bacteriophages in the Poultry Industry

Animals ◽

10.3390/ani10050872 ◽

2020 ◽

Vol 10 (5) ◽

pp. 872 ◽

Cited By ~ 6

Author(s):

Katarzyna Żbikowska ◽

Monika Michalczuk ◽

Beata Dolka

Keyword(s):

Foodborne Pathogens ◽

Pathogenic Bacteria ◽

Bacterial Resistance ◽

Health And Safety ◽

Legal Framework ◽

Multidrug Resistant ◽

Poultry Production ◽

Salmonella Spp ◽

Food Contact Surfaces ◽

Public Health And Safety

The emergence of multidrug-resistant infections and antibiotic failures have raised concerns over human and veterinary medicine worldwide. Poultry production has had to confront the problems of an alarming increase in bacterial resistance, including zoonotic pathogens. According to the European Food Safety Authority (EFSA), campylobacteriosis and salmonellosis have been the most frequently reported human foodborne diseases linked to poultry. This situation has strongly stimulated a renewal of scientists’ interest in bacteriophages (phages) since the beginning of the 21st century. Bacteriophages are the viruses of bacteria. They are abundant in nature, and accompany bacteria in each environment they colonize, including human microbiota. In this review, we focused on the use of bacteriophages as therapeutic agents to treat infections and reduce counts of pathogenic bacteria in poultry, as biocontrol agents to eliminate foodborne pathogens on/in food, and also as disinfectants to reduce contamination on food-contact surfaces or poultry carcasses in industrial conditions. Most of the phage-based products are targeted against the main foodborne pathogens, such as Campylobacter jejuni, Salmonella spp., Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Clostridium perfringens. Phages are currently addressed at all stages of the poultry production "from farm to fork", however, their implementation into live birds and food products still provokes discussions especially in the context of the current legal framework, limitations, as well as public health and safety.

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