2-O-β-d-glucopyranosyl- -ascorbic acid, a novel vitamin C derivative from Lycium barbarum, prevents oxidative stress

Large elastic artery compliance is reduced and arterial blood pressure (BP) is increased in the central (cardiothoracic) circulation with aging. Reactive oxygen species may tonically modulate central arterial compliance and BP in humans, and oxidative stress may contribute to adverse changes with aging. If so, antioxidant administration may have beneficial effects. Young (Y; 26 ± 1 yr, mean ± SE) and older (O; 63 ± 2 yr, mean ± SE) healthy men were studied at baseline and during acute (intravenous infusion; Y: n = 13, O: n = 12) and chronic (500 mg/day for 30 days; Y: n = 10, O: n = 10) administration of ascorbic acid (vitamin C). At baseline, peripheral (brachial artery) BP did not differ in the two groups, but carotid artery compliance was 43% lower (1.2 ± 0.1 vs. 2.1 ± 0.1 mm2/mmHg × 10–1, P < 0.01) and central (carotid) BP (systolic: 116 ± 5 vs. 101 ± 3 mmHg, P < 0.05, and pulse pressure: 43 ± 4 vs. 36 ± 3 mmHg, P = 0.16), carotid augmentation index (AIx; 27.8 ± 7.8 vs. –20.0 ± 6.6%, P < 0.001), and aortic pulse wave velocity (PWV; 950 ± 88 vs. 640 ± 38 cm/s, P < 0.01) were higher in the older men. Plasma ascorbic acid concentrations did not differ at baseline (Y: 71 ± 5 vs. O: 61 ± 7 μmol/l, P = 0.23), increased ( P < 0.001) to supraphysiological levels during infusion (Y: 1,240 ± 57 and O: 1,056 ± 83 μmol/l), and were slightly elevated ( P < 0.001 vs. baseline) with supplementation (Y: 96 ± 5 μmol/l vs. O: 85 ± 6). Neither ascorbic acid infusion nor supplementation affected peripheral BP, heart rate, carotid artery compliance, central BP, carotid AIx, or aortic PWV (all P > 0.26). These results indicate that the adverse changes in large elastic artery compliance and central BP with aging in healthy men are not 1) mediated by ascorbic acid-sensitive oxidative stress (infusion experiments) and 2) affected by short-term, moderate daily ascorbic acid (vitamin C) supplementation.

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The protective role of ascorbic acid (vitamin C) against chlorpyrifos-induced oxidative stress in Oreochromis niloticus

Fish Physiology and Biochemistry ◽

10.1007/s10695-011-9544-6 ◽

2011 ◽

Vol 38 (3) ◽

pp. 635-643 ◽

Cited By ~ 17

Author(s):

Ferbal Özkan ◽

Suna Gül Gündüz ◽

Mehmet Berköz ◽

Arzu Özlüer Hunt ◽

Serap Yalın

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Vitamin C ◽

Oreochromis Niloticus ◽

Protective Role

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Study of oxidative stress biomarkers in obese children

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20184042 ◽

2018 ◽

Vol 6 (10) ◽

pp. 3335

Author(s):

Avni Kanji Fariya ◽

Bina F. Dias

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Ascorbic Acid ◽

Childhood Obesity ◽

Vitamin C ◽

Low Grade ◽

Obese Children ◽

Oxidative Stress Biomarkers ◽

Plasma Ascorbic Acid ◽

Increased Risk

Background: The objective is to study the oxidative stress in obese and non-obese children by assessing the biomarkers of lipid peroxidation and antioxidant status, Malondialdehyde (MDA) and Ascorbic acid (vitamin C) respectively. Childhood obesity is a growing global epidemic that requires attention due to the burden placed on the healthcare system for children and adults. Consumption of fatty foods and a high sugar, fat diet, and no exercise qualify as the main reasons for obesity among children and adults. Childhood obesity is connected with an increased risk of various diseases such as diabetes, cardiovascular, stroke, certain types of cancer later in life, social problems and depression among youths. Obesity is also characterized by chronic low grade inflammation with permanently increased oxidative stress (OS). Over-expression of oxidative stress damages cellular structures together with under-production of anti-oxidant mechanisms, leading to the development of obesity-related complications.Methods: The study involved 25 obese children for Vitamin C, 20 obese children for Malondialdehyde (MDA) and 18 non obese children for both Vitamin C and MDA in the age group of 5-14 years, without any complications. This study was conducted at L.T.M.M College. Plasma Ascorbic Acid was estimated colorimetrically by using 2,6- dicholrophenol indophenol dye and similarly Malondialdehyde was estimated colorimetrically by MDA-TBA colored complex.Results: The study showed significantly higher values of MDA and lower level of Vitamin C in obese children as compared with non-obese children.Conclusions: The levels of lipid peroxidation marker Malondialdehyde (MDA) is higher and level of antioxidant marker Ascorbic Acid (Vitamin C) is lower in obese children as compared with non-obese children. Thereby increasing oxidative stress and hence the oxidative damage to cells.

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The Vitamin C Analogue 2-O-β-D-Glucopyranosyl-L-ascorbic Acid in Rhizomes, Stems and Leaves of Lycium barbarum

CHIMIA International Journal for Chemistry ◽

10.2533/chimia.2020.828 ◽

2020 ◽

Vol 74 (10) ◽

pp. 828-830

Author(s):

Carole Bubloz ◽

Isabelle Udrisard ◽

Fabrice Micaux ◽

Umberto Piantini ◽

Lara Amini-Rentsch ◽

...

Keyword(s):

Ascorbic Acid ◽

Vitamin C ◽

Bioactive Compounds ◽

Dry Weight ◽

Plant Tissues ◽

Lycium Barbarum ◽

Natural Sources ◽

Acid Analogue ◽

Goji Berries ◽

Stems And Leaves

Awareness of health benefits of goji berries coming from their bioactive compounds, mostly antioxidants like ascorbic acid, has grown. Recently, an ascorbic acid analogue from goji berries, the 2-O-β-D-glucopyranosyl-L-ascorbic acid has been reported. In rats, the analogue is absorbed intact and in the form of free vitamin C and consequently has been proposed as a provitamin C. Synthesis of the analogue is demanding and laborious and therefore reliable natural sources are searched. Knowledge concerning the analogue's occurrence in other parts of goji plant is lacking. The aim of this study was to evaluate the contents of 2-O-β-D-glucopyranosyl-L-ascorbic acid in rhizomes, stems and leaves from Lycium barbarum. Rhizomes, stems and leaveswere extracted and the content of 2-O-β-D-glucopyranosyl-L-ascorbic acid and non glucosylated, free ascorbic acid was determined by HPLC-DAD. 2-O-β-D-glucopyranosyl-L-ascorbic acid was found in all goji plant tissues investigated. Based on dry weight, 3.34 mg/100 g were found in the leaves, 4.05 mg/100 g in the stems and up to 12.6 mg/100 g in the rhizomes. Nevertheless, the analogue content in goji berries is much higher (40 to 280 mg/100 g dry weight). The present study confirmed the presence of 2-O-β-D-glucopyranosyl-L-ascorbic acid in rhizomes, stems and leaves of Lycium barbarum. However, their content compared to goji berries is considerably lower.

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Ascorbic acid as a sensor of oxidative stress and a regulator of gene expression: The Yin and Yang of vitamin C

Medical Hypotheses ◽

10.1016/j.mehy.2007.02.008 ◽

2007 ◽

Vol 69 (4) ◽

pp. 953-954 ◽

Cited By ~ 11

Author(s):

Antonella Grano ◽

Mario C. De Tullio

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Ascorbic Acid ◽

Vitamin C ◽

Yin And Yang

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Kolaviron and L-Ascorbic Acid Attenuate Chlorambucil-Induced Testicular Oxidative Stress in Rats

Journal of Toxicology ◽

10.1155/2014/587015 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Ebenezer Tunde Olayinka ◽

Ayokanmi Ore

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Vitamin C ◽

Wistar Rats ◽

Lymphocytic Leukaemia ◽

Protective Capacity ◽

Butanoic Acid ◽

Garcinia Kola ◽

Male Wistar Rats

Chlorambucil (4-[4-[bis(2-chloroethyl)amino]phenyl]butanoic acid) is an alkylating agent, indicated in chronic lymphocytic leukaemia. Kolaviron (KV), a biflavonoid complex fromGarcinia kola,and L-ascorbic acid (AA) are known to protect against oxidative damagein vivo. This study evaluates the protective capacity of KV and AA on chlorambucil-induced oxidative stress in the testes of rat. Twenty male Wistar rats (180–200 g) were randomized into four groups: I: control, II: chlorambucil (0.2 mg/kg b.w.), III: 0.2 mg/kg chlorambucil and 100 mg/kg KV, and IV: 0.2 mg/kg chlorambucil and 100 mg/kg AA. After 14 days of treatments, results indicated that chlorambucil caused significant reduction (P<0.05) in testicular vitamin C and glutathione by 32% and 39%, respectively, relative to control. Similarly, activities of testicular GST, SOD, and CAT reduced significantly by 48%, 47%, and 49%, respectively, in chlorambucil-treated rats relative to control. Testicular MDA and activities of ALP, LDH, and ACP were increased significantly by 53%, 51%, 64%, and 70%, respectively, in the chlorambucil-treated rat. However, cotreatment with KV and AA offered protection and restored the levels of vitamin C, GSH, and MDA as well as SOD, CAT, GST, ACP, ALP, and LDH activities. Overall, kolaviron and L-ascorbic acid protected against chlorambucil-induced damage in the testes of the rat.

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Nickel and Oxidative Stress: Cell Signaling Mechanisms and Protective Role of Vitamin C

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666191205122249 ◽

2020 ◽

Vol 20 (7) ◽

pp. 1024-1031

Author(s):

Swastika Das ◽

Rachamalla C. Reddy ◽

Kailash S. Chadchan ◽

Arun J. Patil ◽

Mallanagouda S. Biradar ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Ascorbic Acid ◽

Vitamin C ◽

Hypoxia Inducible Factor ◽

Protective Role ◽

Leukotriene B4 ◽

Low Oxygen ◽

Gene Expressions

Background: Nickel activates the signaling pathways through the oxygen sensing mechanism and the signaling cascades that control hypoxia-inducible transcriptional gene expressions through oxidative stress. This review emphasizes on the recent updates of nickel toxicities on oxidant and antioxidant balance, molecular interaction of nickel and its signal transduction through low oxygen microenvironment in the in-vivo physiological system. Discussion: ickel alters intracellular chemical microenvironment by increasing ionized calcium concentration, lipid peroxidation, cyclooxygenase, constitutive nitric oxide synthase, leukotriene B4, prostaglandin E2, interleukins, tumor necrosis factor-α, caspases, complement activation, heat shock protein 70 kDa and hypoxia-inducible factor-1α. The oxidative stress induced by nickel is responsible for the progression of metastasis. It has been observed that nickel exposure induces the generation of reactive oxygen species which leads to the increased expression of p53, NF-kβ, AP-1, and MAPK. Ascorbic acid (vitamin C) prevents lipid peroxidation, oxidation of low-density lipoproteins and advanced oxidation protein products. The mechanism involves that vitamin C is capable of reducing ferric iron to ferrous iron in the duodenum, thus the availability of divalent ferrous ion increases which competes with nickel (a divalent cation itself) and reduces its intestinal absorption and nickel toxicities. Conclusion: Reports suggested the capability of ascorbic acid as a regulatory factor to influence gene expression, apoptosis and other cellular functions of the living system exposed to heavy metals, including nickel.

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