Detection of antibiotic residues and association of cefquinome residues with the occurrence of Extended-Spectrum β-Lactamase (ESBL)-producing bacteria in waste milk samples from dairy farms in England and Wales in 2011

2014 ◽  
Vol 96 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Luke Randall ◽  
Katharina Heinrich ◽  
Robert Horton ◽  
Lucy Brunton ◽  
Matthew Sharman ◽  
...  
Keyword(s):  
Dairy Farms ◽  
Antibiotic Residues ◽  
England And Wales ◽  
Milk Samples ◽  
Extended Spectrum ◽  
Waste Milk

A survey of antimicrobial usage on dairy farms and waste milk feeding practices in England and Wales

2012 ◽  
Vol 171 (12) ◽  
pp. 296-296 ◽  
Author(s):  
L. A. Brunton ◽  
D. Duncan ◽  
N. G. Coldham ◽  
L. C. Snow ◽  
J. R. Jones
Keyword(s):  
Dairy Farms ◽  
Feeding Practices ◽  
England And Wales ◽  
Antimicrobial Usage ◽  
Waste Milk ◽  
Milk Feeding

Presence of Mycobacterium avium subs. paratuberculosis DNA in milk used to feed calves in Portugal

2017 ◽  
Vol 84 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Célia Leão ◽  
Ana Botelho ◽  
Elisabete Martins ◽  
Carla Aguiar ◽  
Inês Rebelo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Disease Transmission ◽  
Dairy Farms ◽  
Mitigation Strategies ◽  
Male And Female ◽  
Milk Samples ◽  
Single Origin ◽  
Waste Milk ◽  
Potential Risks ◽  
Bulk Tank

This Technical Research communication describes results of a study aimed at detecting the presence of Map in milk fed to calves, and identifying possible risk factors for that presence. A questionnaire was performed on 37 dairy farms and waste milk samples were collected on 3 occasions separated by a minimum of 1 week. For farms not feeding waste milk, bulk tank milk samples were collected instead. A real time PCR for the detection of the IS900 sequence was performed for the detection of Map. A majority of farms (89·2%) fed waste milk, with only one pasteurising the milk before feeding it to calves. Results of the PCR showed that 51·5% of the farms that were feeding waste milk had a positive result for Map on that milk. None of the studied risk factors were significantly associated with the presence of Map in milk samples, possibly due to the small number of farms entering the study. However, the prevalence of positive samples for Map on PCR was 3·5 times higher for farms that bought in animals from a single origin and 1·9 times higher for farms that bought from multiple farms, when compared with closed farms. Having a calving area for multiple cows also increased the risk of a positive Map result by 1·5 when compared with single pens. The risk of having a positive Map result on waste milk was 1·6 times higher for farms feeding that milk to male calves and 1·4 for farms feeding to both male and female calves, when compared with farms not feeding waste milk. This study highlights paratuberculosis as one of the potential risks of feeding waste milk to calves, and the need for mitigation strategies to be in place to avoid unnecessary disease transmission.

Characterisation of AmpC and extended-spectrum beta-lactamase producing E. coli from New Zealand dairy farms

2021 ◽  
Vol 117 ◽  
pp. 104998
Author(s):  
Sara A. Burgess ◽  
Jacinda Aplin ◽  
Patrick J. Biggs ◽  
Georgia Breckell ◽  
Jackie Benschop ◽  
...  
Keyword(s):  
New Zealand ◽  
Dairy Farms ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

Characterization of the microbiota and resistome of bulk tank milk samples from Prince Edward Island dairy farms

2021 ◽  
Author(s):  
Landon M.C. Warder ◽  
Enrique Doster ◽  
Jennifer K. Parker ◽  
Paul S. Morley ◽  
J.T. McClure ◽  
...  
Keyword(s):  
Prince Edward Island ◽  
Dairy Farms ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Occurrence of aflatoxin M1 in bovine milk and associated risk factors among dairy farms of Punjab, India

2021 ◽  
pp. 1-10
Author(s):  
H. Thukral ◽  
P. Dhaka ◽  
J. Singh Bedi ◽  
R. Singh Aulakh
Keyword(s):  
Risk Factors ◽  
European Union ◽  
Bovine Milk ◽  
Dairy Farms ◽  
Buffalo Milk ◽  
Cow Milk ◽  
Milk Samples ◽  
Significant Difference ◽  
Associated Risk Factors ◽  
Mean Concentration

Aflatoxin M1 (AFM1) contamination in milk and milk products may pose a major public health concern. The present cross-sectional study was aimed to estimate the prevalence of AFM1 in bovine milk across all districts of Punjab, India and to identify the associated animal and farm level risk factors. A total of 402 milk samples (266 cow milk and 136 buffalo milk) were analysed using commercial ELISA and representative samples were confirmed using HPLC-FLD. The results revealed that 56.2 and 13.4% of the milk samples exceeded the maximum levels of the European Union, i.e. 0.05 μg/l and Food Safety and Standards Authority of India (FSSAI), i.e. 0.5 μg/l for AFM1 in milk, respectively. On analysis of species variation, buffalo milk (prevalence: 56.6%; mean concentration: 0.42±0.9 μg/l) was found to have higher AFM1 levels than cow milk (prevalence: 56.0%; mean concentration: 0.19±0.3 μg/l), with statistically significant difference between mean concentrations (P<0.01) and non-significant difference between AFM1 prevalence (P=0.91). Furthermore, milk from commercial dairy farms (prevalence: 64.7%; mean concentration: 0.34±0.65 μg/l) was found to be more contaminated than from household dairy establishments (prevalence: 47.8%; mean concentration: 0.19±0.65 μg/l). The risk factors ‘above average milk yield/day’ (odds ratio (OR): 2.4) and ‘poor animal hygiene’ (OR: 1.9) were identified at animal level, and ‘intensive dairy farming’ (OR: 3.1) and ‘animal feed without aflatoxin binder’ (OR: 4.7) as farm level risk factors for AFM1 excretion above maximum levels of European Union in milk. Among cow breeds, the milk from ‘non-descript’ breed (OR: 11.5) was found to be most contaminated with AFM1 and the least from Jersey breed (OR: 1.0). The present study highlighted the presence of AFM1 in milk samples; therefore, regular monitoring of AFM1 in milk is required so that high risk regions and associated risk factors can be addressed appropriately.

The Post-quota performance of dairy farms in england and wales

1990 ◽  
Vol 22 (10) ◽  
pp. 1399-1406 ◽  
Author(s):  
P. J. Dawson ◽  
B. White
Keyword(s):  
Dairy Farms ◽  
England And Wales

Performance of Various Tests Used to Screen Antibiotic Residues in Milk Samples from Individual Animals

1994 ◽  
Vol 77 (4) ◽  
pp. 862-870 ◽  
Author(s):  
James S Cullor ◽  
Alison Van Eenennaam ◽  
Ian Gardner ◽  
Lynn Perani ◽  
Jon Dellbvger ◽  
...  
Keyword(s):  
False Positive ◽  
Dairy Industry ◽  
Initial Therapy ◽  
Treatment Withdrawal ◽  
Quality Assurance Program ◽  
Antibiotic Residues ◽  
Withdrawal Time ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Abstract The 10-point Milk and Dairy Beef Quality Assurance Program was developed collaboratively by the National Milk Producers Federation and the American Veterinary Medical Association and is designed to promote and document the responsible use of antibiotics in the dairy industry. One area of emphasis in this program is testing of individual animals for antibiotic residues after a specified post-treatment withdrawal time. We examined the performance of various assay systems on milk samples from individual cows. These assays are used at present on bulk tank milk samples by regulatory agencies, processing plants, producers, and veterinarians to detect the presence of β-lactam antibiotics. A high proportion of false-positive results was obtained for both the pretreatment milk samples from cows with clinical mastitis and the milk samples obtained 21 days after initial therapy (nonantibiotic and antibiotic) for the treatment of mastitis. A high proportion of false-positive outcomes was obtained from the milk of clinically normal cows that had not received any medication for at least 30 days prior to evaluation. The results indicate a serious problem in the use of some assays that were designed to evaluate residues bulk tank milk samples to analyze samples from individual cows. This error in assay specificity results in the unjustifiable discarding of milk that meets regulatory standards and may be misused to accuse the producer or veterinarian of not adhering to regulatory guidelines. Maintaining a safe, high-quality milk supply is a constant goal of the dairy industry, which must be provided the appropriate tools and techniques to meet this challenge.

Microbiological Screening Tests to Detect Antibiotic Residues in Cull Dairy Cows

1987 ◽  
Vol 50 (2) ◽  
pp. 97-102 ◽  
Author(s):  
JOSEPH P. TRITSCHLER ◽  
ROBERT T. DUBY ◽  
STEPHEN P. OLIVER ◽  
ROBERT W. PRANGE
Keyword(s):  
Bacillus Subtilis ◽  
Dairy Cows ◽  
Dairy Farms ◽  
Urine Samples ◽  
Screening Tests ◽  
Antibiotic Residues ◽  
Live Animal ◽  
Subsequent Investigation ◽  
On Farm ◽  
Microbiological Procedures

Two microbiological procedures were evaluated to detect antibiotic residues in dairy cows at slaughter. Inhibition of Bacillus subtilis growth was used for determining the presence or absence of antibiotic residues. The two tests differed only in the concentration of B. subtilis used. The Swab Test on Premises (STOP) was used to detect antibiotic residues in kidney and muscle tissue and the Live Animal Swab Test (LAST) was used to detect residues in urine of cull dairy cows. Kidney samples from 3% of cull dairy cows were positive. Confirmation by standard reference procedures and a subsequent investigation on antibiotic residues in urine from 317 cows and heifers with known antibiotic treatment histories suggest that a high percentage of false-positive readings occurred in urine. In addition, 23 % of urine samples were difficult to interpret in that B. subtilis growth surrounding Swabs dipped in urine was reduced. While producer response was generally favorable for an on-farm screening test for antibiotic residues detection in cows going to slaughter, interpretation problems, difficulty in collecting urine samples, and concerns over the complexity and sensitivity make it unlikely that the acceptance of the LAST will be widespread on dairy farms.

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