Phytochemical analysis and antioxidant activity of leaf extracts from Morella serrata (Lam.) Killick (Myricaceae)

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions.

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Antioxidant Activity and Phenolic Content of Bergenia crassifolia, B. x ornata and B. ciliata

Natural Product Communications ◽

10.1177/1934578x1400900424 ◽

2014 ◽

Vol 9 (4) ◽

pp. 1934578X1400900 ◽

Cited By ~ 2

Author(s):

Helena Hendrychová ◽

Anna Vildová ◽

Nina Kočevar-Glavač ◽

Lenka Tůmová ◽

Elnura Abdykerimova Kanybekovna ◽

...

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Environmental Influence ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Different Seasons ◽

Bergenia Crassifolia ◽

Radical Scavenging Properties

This study focused on a phytochemical analysis of Bergenia crassifolia (L.) Fritsch., B. ciliata (Haw.) Sternb., and B. x ornata Stein. and evaluation of their free radical scavenging properties. Arbutin and total tannin contents of the leaves of the Bergenia species were determined during different seasons. The present study also aimed at analyzing, for the first time, environmental influence on concentrations of phenolic metabolites in Bergenia leaves. The highest total tannin content was found in the leaves of B. crassifolia (24.9 – 48.7 mg.g−1 DW) and B. x ornata (36.9 mg.g−1 DW). The highest amount of arbutin was in the leaves of B. x ornata (35.8 – 51.0 mg.g−1 DW) and B. crassifolia (24.6 – 41.7 mg.g−1 DW). Autumn was better than spring for the collection of Bergenia leaves for the highest amount of arbutin ( B. x ornata: 51.0 mg.g−1 DW). Free radical scavenging potential, in DPPH and ABTS assays, of the water leaf extracts revealed that extracts of B. crassifolia and B. x ornata are the most active radical scavengers. Antioxidant activity correlated well with the content of total tannin, especially in the ABTS assay, which suggests an important role for these compounds in antioxidant activity. It was shown that phenolic concentrations in Bergenia leaves are affected by seasonal factors. A significant correlation was found between arbutin and tannin contents and the average humidity.

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Total Phenolic Contents and Antioxidant Properties of Algerian Arbutus unedo L. Extracts

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i3-s.4182 ◽

2020 ◽

Vol 10 (3-s) ◽

pp. 159-168

Author(s):

Saliha Laouicha ◽

Abderrahmane Senator ◽

Abdallah Kherbache ◽

Hamama Bouriche

Keyword(s):

Antioxidant Activity ◽

Folk Medicine ◽

Antioxidant Properties ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Arbutus Unedo ◽

Phenolic Contents ◽

Antioxidant Agents ◽

Ic50 Values

Arbutus unedo L. is a plant widely distributed in the Mediterranean basin and North Africa, frequently used in traditional folk medicine to treat diabetes and arterial hypertension. This study aims to evaluate the phenolic composition and antioxidant activity of ethyl acetate (EA.E) and n-butanolic (But.E) extracts prepared from leaves of Arbutus unedo. Total phenolic and flavonoids contents were determined spectrophotometrically. The antioxidant activity was evaluated using DPPH•, OH•, H2O2, O•-2, ß-carotene bleaching and AAPH-induced erythrocyte oxidative hemolysis assays. The phytochemical analysis showed the presence of polyphenols and flavonoids in both extracts. The high amount was observed in EA.E which exerted the stronger antioxidant effect, with IC50 values of 3.43 μg/mL, 323.45 µg/mL, 38.40 µg/mL and 11.56 µg/mL, in DPPH•, OH•, O•-2 and H2O2 tests, respectively. Both extracts inhibited β-carotene bleaching, but EA.E is always more potent (92%) than But.E (85%). Furthermore, the EA.E showed the highest protective effect on erythrocyte hemolysis induced by AAPH, with half time hemolysis (HT50) of 122.02 min at 40 µg/mL. Taken together, this study showed that Arbutus unedo leaf extracts possess strong antioxidant potential, which may be attributed to the presence of a high amount of polyphenolic constituents. So, this plant might be exploited as a potential source of natural antioxidant agents for pharmaceutical and food applications. Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo.

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PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF CLERODENDRUM PANICULATUM (L.) LEAF AND FLOWER EXTRACTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2022.v15i1.43361 ◽

2021 ◽

pp. 31-34

Author(s):

DEEPA R HEBBAR ◽

DEVIKA M ◽

RASHMI GN ◽

NALINI MS

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Scavenging Activity ◽

Leaf Extracts ◽

Hexane Extracts ◽

Reducing Power Assay

Objective: This study was designed to evaluate the phytochemicals present in the flower and leaf extracts of Clerodendrum paniculatum L., collected from Nelji village of Kodagu district . Methods: The healthy leaves and flowers of C. paniculatum were collected and the plant extracts were prepared using ethanol, hexane and distilled water separately. Phytochemical analysis was conducted using standard procedurs for the flower and leaf extracts of C. paniculatum. The antioxidant activity in leaf and flower extracts was determined by three assays, estimation of total phenolic content, reducing power assay and radical scavenging activity (DPPH) using standard procedures. Results: Phytochemical screening conducted for the flower and leaf extracts of C. paniculatum showed the presence of three phytochemicals, namely saponins, alkaloids and terpenoids. Terpenoids were commonly present in all the extracts of flower and leaf that is in both polar (aqueous and ethanol) solvent and in non-polar (hexane) solvents. The extracts tested for the antioxidant activity showed the presence of total phenolics in ethanol, aqueous and hexane extracts. The aqueous extract showed high redox potential followed by ethanol and hexane extracts. The aqueous leaf extract showed high radical scavenging activity when compared to the flower extracts of C. paniculatum. Conclusion: The present study showes C. paniculatum to be an important medicinal plant, since the flower and leaves showed good antioxidant activity. Thus it may used in the treatment of diseases and may also used in the preparation of natural or herbal drugs due to the presence of antioxidants.

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IN VITRO EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AN INDIAN MEDICINAL PLANT: CELASTRUS PANICULATUS WILLD

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v9i1.726 ◽

2020 ◽

Vol 9 (1) ◽

Author(s):

Pradeep K Naik ◽

Debasmita Dubey ◽

Priya Cheruvanachari ◽

Animesh Pattnaik ◽

Swaraj K Babu

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Antioxidant Properties ◽

Antibacterial Properties ◽

Resistant Bacteria ◽

Dilution Method ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Dpph Method ◽

Celastrus Paniculatus

Aim: To evaluate the antibacterial efficacy, the presence of different phytoconstituents of the leaf extracts of Indian plant Celastrus paniculatus against 6 antibiotic-resistant bacteria along with the evaluation of their antioxidant potentiality. Methods: The leaf extracts were prepared using 4 solvents, water, methanol, n-butanol, and acetone. The antibacterial property was evaluated using agar well diffusion and micro-broth dilution method. Qualitative phytochemical analysis was performed using standard protocols for each solvent extract to check for the presence of the secondary metabolites and phytochemicals which attributes to the antibacterial effectivity of C. paniculatus. Further, the antioxidant potentiality of all the 4 leaf-solvent-extracts was estimated using the DPPH method. Results: The n-butanol leaf extract exhibited the highest antibacterial and antioxidant activity, followed by acetone, methanol extracts. The aqueous extracts exhibited negligible antibacterial activity. Conclusion: This study validated the antibacterial properties of C. paniculatus leaf extracts against both gram-positive and negative bacteria and the phytochemical analysis revealed the presence of many secondary importance which can be attributed to its therapeutic properties. Hence, this plant can be further used as a complementary or alternative choice of drug for combatting multidrug-resistant bacterial pathogens. Keywords: Antibiotic resistance; Medicinal plants; Celastrus Paniculatus; Antibacterial activity, Phytochemical Analysis; Antioxidant activity

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Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

International Journal of Food Science ◽

10.1155/2020/3436940 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Adelina Simamora ◽

Adit W. Santoso ◽

Kris H. Timotius ◽

Ika Rahayu

Keyword(s):

Antioxidant Activity ◽

Xanthine Oxidase ◽

Metabolic Diseases ◽

Antioxidant Properties ◽

Reducing Power ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Food Ingredient ◽

Pbr322 Plasmid ◽

Total Antioxidant

Premna serratifolia, commonly known as Arogo in Tentena-Sulawesi, is a popular vegetable. As a promising herbal tea and food ingredient, further investigation is required to find the best knowledge for medicinal use of P. serratifolia leaves. This research investigated the antioxidant activity of the ethanol (EEPS) and water (WEPS) extracts of P. serratifolia leaves, based on their scavenging activities on DPPH radicals and their reducing capacities (CuPRAC, total antioxidant/phosphomolybdenum, and ferric thiocyanate reducing power assays). The DNA-protecting effect by EEPS was tested using pBR322 plasmid DNA against •OH radical-induced damage. The inhibition potentials of both extracts against several enzymes related to metabolic diseases (α-glucosidase, α-amylase, xanthine oxidase, and protease) were evaluated. The phytochemical analysis was conducted by an LC-QTOF-MS/MS technique. EEPS proved to be a better antioxidant and had higher phenolic content compared to WEPS. EEPS demonstrated a protective effect on DNA with recovery percentage linearly correlated with EEPS concentrations. Strong inhibition on α-glucosidase and α-amylase was observed for EEPS; however, EEPS and WEPS showed weak inhibitions on xanthine oxidase and protease. LC-QTOF-MS/MS analysis identified seven main components in EEPS, namely scroside E, forsythoside A and forsythoside B, lavandulifolioside, diosmin, nobilin D, campneoside I, and isoacteoside. These components may be responsible for the observed enzymes inhibitions and antioxidant properties. Premna serratifolia leaves can be an appropriate choice for the development of nutraceutical and drug preparations.

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Phytochemical Analysis and Evaluation of Antimicrobial Activity of Peumus boldus, Psidium guajava, Vernonia polysphaera, Persea Americana, Eucalyptus citriodora Leaf Extracts and Jatropha multifida Raw Sap

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190409104910 ◽

2019 ◽

Vol 20 (5) ◽

pp. 433-444 ◽

Cited By ~ 1

Author(s):

Jhonatas Emílio Ribeiro da Cruz ◽

Joyce Ferreira da Costa Guerra ◽

Marcos de Souza Gomes ◽

Guilherme Ramos Oliveira e Freitas ◽

Enyara Rezende Morais

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Medicinal Plants ◽

Psidium Guajava ◽

Persea Americana ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Eucalyptus Citriodora ◽

Leaf Extracts ◽

Jatropha Multifida

Background: Currently, the treatment of infectious diseases has not always been successful due to the emergence of microbial resistance worldwide. Objective: This study aimed to evaluate the antioxidant activity, content of total phenolic compounds and flavonoids, antifungal potential and antibacterial action of six medicinal plants found in the Cerrado, leaf extracts of Boldo (Peumus boldus), Goiaba (Psidium guajava), Assa-Peixe (Vernonia polysphaera), Abacate (Persea americana), Eucalipto (Eucalyptus citriodora) and raw sap of Bálsamo (Jatropha multifida). Methods: The antioxidant activity was also determined through the DPPH, ABTS and phosphomolybdenum assays. In addition, the total phenolic content and flavonoid dosage were analyzed using the Folin- Ciocalteu method and the aluminum chloride test, respectively. Results: All extracts, except from Assa-Peixe, showed promising values against Staphylococcus aureus, with halos varying from 13-20 mm. Analysis of the minimum inhibitory concentration (MIC) values of the six medicinal plants revealed inhibitory activity of S. aureus, with concentrations varying from 3.12-12.5 mg/mL, which is a significant result considering that S. aureus is one of the main causes of hospital infections. Conclusion: In the analysis of the phytochemical profile, Goiaba contained the best yield of phenolic compounds and total flavonoids, as well as higher antioxidant activity by DPPH and phosphomolybdenum, demonstrating that this species contains antioxidant components that can sequester free radicals under in vitro conditions. Therefore, the crude extracts investigated are promising and their antibacterial and antioxidant actions should be thoroughly studied.

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Proximate, Phytochemicals and Reducing Power of Leaf Extracts of Colocasia esculenta and Ipomoea batatas

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2019/v28i430158 ◽

2020 ◽

pp. 1-11

Author(s):

Achadu Abraham Eleojo ◽

Umeh Chisom Charles ◽

Mohammed Nimat

Keyword(s):

Antioxidant Activity ◽

Ipomoea Batatas ◽

Reducing Power ◽

Sodium Content ◽

Colocasia Esculenta ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Calcium Iron ◽

Analysis Determination

Background: Reports has shown that edible leaves of vegetable plants serve both nutritional and medicinal purposes, yet are poorly utilized due to inadequate enlightenment of the major populace on their compositions. Aims: The aim of this study was to determine the proximate, phytochemicals and reducing power of leaf extracts of Colocasia esculenta and Ipomoea batatas. Methodology: Washed and air dried leaf samples were milled and subjected to proximate and phytochemical analysis. Determination of calcium, iron and sodium content was by Atomic absorption spectroscopy. Reducing power was determined by the Potassium ferricyanide reducing power method. Results: Ipomoea batatas leaves was found to be higher in moisture (14.05%) and carbohydrate (29.33%) while Colocasia esculenta leaves was higher in ash (10.00%), Crude fiber (16.27%), Fat (10.17%) and protein (29.41%). A better antioxidant activity and higher levels of all phytochemicals and minerals were observed in leaves of Colocasia esculenta compared to leaves of Ipomoea batatas. Conclusion: This study suggests that both leaves are of importance to human nutrition considering the observed levels of nutrients, phytochemicals and antioxidant activity. While C. esculenta leaves should be preferred for its nutrient and antioxidant advantages, both leaves can contribute immensely to the daily nutrient requirements.

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Phytochemical analysis and antioxidant activity of leaf extracts of some selected plants of the family Acanthaceae

Plant Science Today ◽

10.14719/pst.2020.7.2.717 ◽

2020 ◽

Vol 7 (2) ◽

pp. 264

Author(s):

Kshetrimayum Kripasana ◽

Jobi Xavier

Keyword(s):

Antioxidant Activity ◽

Linear Regression Analysis ◽

Antioxidant Property ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Ic50 Value ◽

Dpph Method ◽

Phytochemical Compounds ◽

Dpph Scavenging ◽

Immense Potential

The present era of scientific research has witnessed an enumerable amount of evidences to showcase the immense potential of medicinal plants. In the present investigation, the phytochemical analysis of Phlogacanthus pubinervius T. Anderson., Adhatoda vasica (L.) Nees,Phlogacanthus thyrsiflorus Nees, Phlogacanthus curviflorus (Wall.) Nees, and Ruellia tuberosa L. was carried out for the different plants extracted with methanol. Analysis was carried out to estimate the quantity of phenols, carbohydrates, tannins, flavonoids and proteins. The antioxidant property of these plants were analysed using DPPH method. The concentration of the plant samples required to decrease the DPPH concentration by 50% was calculated by interpolation from linear regression analysis and denoted IC50 value (µg/ml). The qualitative analysis showed the presence of alkaloids, tannins, saponins, proteins, carbohydrate and phenols in all the sample extracts. The highest amount of tannins and phenols was observed in P. thyrsiflorus. P. pubinervius (77.83%), A. vasica (74.81%), P. curviflorus (94.20%), and R. tuberosa (70.78%) which showed highest antioxidant activity of DPPH-scavenging at 150 µg/ml of methanol extract. The high percent of scavenging activities of those plants add value to their medicinal properties. The presence of the high amount of phytochemical compounds suggests that the plants have high amount of medicinal compounds and can be extensively used to extract the natural compounds.

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