scholarly journals Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Adelina Simamora ◽  
Adit W. Santoso ◽  
Kris H. Timotius ◽  
Ika Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Xanthine Oxidase ◽  
Metabolic Diseases ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Food Ingredient ◽  
Pbr322 Plasmid ◽  
Total Antioxidant

Premna serratifolia, commonly known as Arogo in Tentena-Sulawesi, is a popular vegetable. As a promising herbal tea and food ingredient, further investigation is required to find the best knowledge for medicinal use of P. serratifolia leaves. This research investigated the antioxidant activity of the ethanol (EEPS) and water (WEPS) extracts of P. serratifolia leaves, based on their scavenging activities on DPPH radicals and their reducing capacities (CuPRAC, total antioxidant/phosphomolybdenum, and ferric thiocyanate reducing power assays). The DNA-protecting effect by EEPS was tested using pBR322 plasmid DNA against •OH radical-induced damage. The inhibition potentials of both extracts against several enzymes related to metabolic diseases (α-glucosidase, α-amylase, xanthine oxidase, and protease) were evaluated. The phytochemical analysis was conducted by an LC-QTOF-MS/MS technique. EEPS proved to be a better antioxidant and had higher phenolic content compared to WEPS. EEPS demonstrated a protective effect on DNA with recovery percentage linearly correlated with EEPS concentrations. Strong inhibition on α-glucosidase and α-amylase was observed for EEPS; however, EEPS and WEPS showed weak inhibitions on xanthine oxidase and protease. LC-QTOF-MS/MS analysis identified seven main components in EEPS, namely scroside E, forsythoside A and forsythoside B, lavandulifolioside, diosmin, nobilin D, campneoside I, and isoacteoside. These components may be responsible for the observed enzymes inhibitions and antioxidant properties. Premna serratifolia leaves can be an appropriate choice for the development of nutraceutical and drug preparations.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

2018 ◽  
Vol 11 (6) ◽  
pp. 136
Author(s):  
Chandrasekaran Swaminathan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Root Extracts ◽  
Wide Range ◽  
Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions. 

scholarly journals Total Phenolic Contents and Antioxidant Properties of Algerian Arbutus unedo L. Extracts

2020 ◽  
Vol 10 (3-s) ◽  
pp. 159-168
Author(s):  
Saliha Laouicha ◽  
Abderrahmane Senator ◽  
Abdallah Kherbache ◽  
Hamama Bouriche
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Arbutus Unedo ◽  
Phenolic Contents ◽  
Antioxidant Agents ◽  
Ic50 Values

Arbutus unedo L. is a plant widely distributed in the Mediterranean basin and North Africa, frequently used in traditional folk medicine to treat diabetes and arterial hypertension. This study aims to evaluate the phenolic composition and antioxidant activity of ethyl acetate (EA.E) and n-butanolic (But.E) extracts prepared from leaves of Arbutus unedo. Total phenolic and flavonoids contents were determined spectrophotometrically. The antioxidant activity was evaluated using DPPH•, OH•, H2O2, O•-2, ß-carotene bleaching and AAPH-induced erythrocyte oxidative hemolysis assays. The phytochemical analysis showed the presence of polyphenols and flavonoids in both extracts. The high amount was observed in EA.E which exerted the stronger antioxidant effect, with IC50 values of 3.43 μg/mL, 323.45 µg/mL, 38.40 µg/mL and 11.56 µg/mL, in DPPH•, OH•, O•-2 and H2O2 tests, respectively. Both extracts inhibited β-carotene bleaching, but EA.E is always more potent (92%) than But.E (85%). Furthermore, the EA.E showed the highest protective effect on erythrocyte hemolysis induced by AAPH, with half time hemolysis (HT50) of 122.02 min at 40 µg/mL. Taken together, this study showed that Arbutus unedo leaf extracts possess strong antioxidant potential, which may be attributed to the presence of a high amount of polyphenolic constituents. So, this plant might be exploited as a potential source of natural antioxidant agents for pharmaceutical and food applications. Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo.

scholarly journals PHYTOCHEMICAL SCREENING AND EVALUATION OF LEAF PARTS OF HYPOESTES PHYLLOSTACHYA ROSEA FOR IN VITRO ANTIOXIDANT ACTIVITIES

2020 ◽  
pp. 54-58
Author(s):  
JENSON JACOB ◽  
MEENU JOHN
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Standard Procedure ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus ◽  
Total Antioxidant ◽  
Phytochemical Compounds ◽  
Reducing Power Assay

Objective: The aim of this study was focussed on phytochemical analysis, in vitro antioxidant activities of different solvent extracts of Hypoestes phyllostachya leaf. Methods: The leaves were washed efficiently, shade dried and coarsely powdered. The powdered leaf material of Hypoestes phyllostachya was successively extracted with hexane, chloroform, methanol and water using Soxhlet apparatus. Preliminary phytochemical screenings for polyphenolics, proteins, phytosteroids, flavonoids, tannins, glycosides, alkaloids carbohydrates, polyphenolics, saponins were done by following standard procedure. In vitro antioxidant activities of various solvent extracts were assessed using DPPH, ABTS, Hydroxy radical, total antioxidant capacity and reducing power assay. Polyphenolics and flavonoids were also estimated. Results: The results showed that most of the phytochemical compounds such as carbohydrates, proteins, steroids, fixed oils and fatty acids, flavonoids, alkaloids, saponins, tannins, polyphenolics and glycosides present in the methanolic and aqueous extract of Hypoestes phyllostachya. The in vitro antioxidant activity of Hypoestes phyllostachya leaf clearly showed that it has better antioxidant activity, which was confirmed by different assays. Conclusion: From this work, it can be concluded that Hypoestes phyllostachya has the potential to be a good antioxidant but have only a lesser amount of flavonoids and polyphenolic contents. This was the first report on the pharmacological activity of the Hypoestes phyllostachya.

scholarly journals Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India

2021 ◽  
Vol 33 (9) ◽  
pp. 2237-2243
Author(s):  
Pooja N. Akhand ◽  
Veena Sharma ◽  
Anupam K. Pathak
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Central India ◽  
Total Phenolic ◽  
Climatic Zones ◽  
Phenolic Contents ◽  
Total Antioxidant ◽  
Sphaeranthus Indicus

In present study, the total phenolic, flavonoid, tannin contents and the antioxidant activity of various solvent extracts of Sphaeranthus indicus collected from three different regions of central India were assessed. Plants extracts were prepared using Soxhlation method, while the total phenols, flavonoids and tannins were measured by the spectrophotometric method. DPPH, metal chelating, nitric oxide, superoxide oxide scavenging activity and FRAP, reducing power, total antioxidant assays were also evaluated. The highest phenolic contents 268.22 GAE equivalent, flavonoids 441.33 QE equivalents and tannin content was 120.32 tannic acid equivalents (mg/g) obtained from ethanolic extracts of sample SIEE-1 compared to other two plants extract. Similarly, the highest flavonoid contents was observed in SIAE-1 aqueous extract and lowest in SIAE-2 and SIAE-3. Extract of SIEE-1 possessed maximum antioxidant potentiality and SIAE-2 shown the least antioxidant activity in all assays. It could be concluded that different agroclimatic conditions have effects on the total phenolics, flavonoids, tannin contents and antioxidant potentiality of S. indicus plant.

scholarly journals PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF CLERODENDRUM PANICULATUM (L.) LEAF AND FLOWER EXTRACTS

2021 ◽  
pp. 31-34
Author(s):  
DEEPA R HEBBAR ◽  
DEVIKA M ◽  
RASHMI GN ◽  
NALINI MS
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Hexane Extracts ◽  
Reducing Power Assay

Objective: This study was designed to evaluate the phytochemicals present in the flower and leaf extracts of Clerodendrum paniculatum L., collected from Nelji village of Kodagu district . Methods: The healthy leaves and flowers of C. paniculatum were collected and the plant extracts were prepared using ethanol, hexane and distilled water separately. Phytochemical analysis was conducted using standard procedurs for the flower and leaf extracts of C. paniculatum. The antioxidant activity in leaf and flower extracts was determined by three assays, estimation of total phenolic content, reducing power assay and radical scavenging activity (DPPH) using standard procedures. Results: Phytochemical screening conducted for the flower and leaf extracts of C. paniculatum showed the presence of three phytochemicals, namely saponins, alkaloids and terpenoids. Terpenoids were commonly present in all the extracts of flower and leaf that is in both polar (aqueous and ethanol) solvent and in non-polar (hexane) solvents. The extracts tested for the antioxidant activity showed the presence of total phenolics in ethanol, aqueous and hexane extracts. The aqueous extract showed high redox potential followed by ethanol and hexane extracts. The aqueous leaf extract showed high radical scavenging activity when compared to the flower extracts of C. paniculatum. Conclusion:  The present study showes C. paniculatum to be an important medicinal plant, since the flower and leaves showed good antioxidant activity. Thus it may used in the treatment of diseases and may also used in the preparation of natural or herbal drugs due to the presence of antioxidants.

IN VITRO EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AN INDIAN MEDICINAL PLANT: CELASTRUS PANICULATUS WILLD

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Pradeep K Naik ◽  
Debasmita Dubey ◽  
Priya Cheruvanachari ◽  
Animesh Pattnaik ◽  
Swaraj K Babu
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Dpph Method ◽  
Celastrus Paniculatus

Aim: To evaluate the antibacterial efficacy, the presence of different phytoconstituents of the leaf extracts of Indian plant Celastrus paniculatus against 6 antibiotic-resistant bacteria along with the evaluation of their antioxidant potentiality. Methods: The leaf extracts were prepared using 4 solvents, water, methanol, n-butanol, and acetone. The antibacterial property was evaluated using agar well diffusion and micro-broth dilution method. Qualitative phytochemical analysis was performed using standard protocols for each solvent extract to check for the presence of the secondary metabolites and phytochemicals which attributes to the antibacterial effectivity of C. paniculatus. Further, the antioxidant potentiality of all the 4 leaf-solvent-extracts was estimated using the DPPH method. Results: The n-butanol leaf extract exhibited the highest antibacterial and antioxidant activity, followed by acetone, methanol extracts. The aqueous extracts exhibited negligible antibacterial activity. Conclusion: This study validated the antibacterial properties of C. paniculatus leaf extracts against both gram-positive and negative bacteria and the phytochemical analysis revealed the presence of many secondary importance which can be attributed to its therapeutic properties. Hence, this plant can be further used as a complementary or alternative choice of drug for combatting multidrug-resistant bacterial pathogens. Keywords: Antibiotic resistance; Medicinal plants; Celastrus Paniculatus; Antibacterial activity, Phytochemical Analysis; Antioxidant activity

scholarly journals PHYTOCHEMICAL PROFILING USING LC-Q-TOF-MS ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A RARE SALT-SECRETING MANGROVE AEGIALITIS ROTUNDIFOLIA ROXB. LEAVES EXTRACT

2019 ◽  
pp. 37-47
Author(s):  
Debjit Ghosh ◽  
Sumanta Mondal ◽  
Prof. Karipeddi Ramakrishna
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Aegialitis Rotundifolia ◽  
Tof Ms

Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro. Methods: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The antioxidant properties were investigated comprehensively using seven in vitro models viz., 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, superoxide (SOD) radical scavenging, lipid peroxidation (LPO) assay, reducing power (RP), and total antioxidant activity. Results: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC-Q-TOF-MS analysis detected 25 phytoconstituents in the extract mostly belonging to flavonoids and alkaloids. The test extract showed strong dose-dependent antioxidant activity in all the seven in vitro models, however, the activity of the extracts was slightly lower compared to the reference standard ascorbic acid. Conclusion: The test extract showed strong antioxidant properties which could be possibly due to the phytoconstituents detected in the extract.

scholarly journals Antioxidant Properties and Effect of Ethanolic Extract of Pulcria crispa on Biochemical and Hematological Parameters of Albino Rats

2020 ◽  
pp. 14-22
Author(s):  
Haytham M. Daradka ◽  
Ramadan Al- Shdefat ◽  
Areej A. Eskandrani
Keyword(s):  
Antioxidant Activity ◽  
Hematological Parameters ◽  
Antioxidant Properties ◽  
Weight Ratio ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Behavioral Changes ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Different Doses

Background: Pulcria crispa (P. crispa) is an herbal plant traditionally used to treat common ailments. Objective: In this study, we investigated P. crispa for its phytochemical constituents, antioxidant properties and effects on biochemical and hematological parameters as well as safety in albino rats. Methods: Phytochemical analysis of ethanolic extract of P. crispa was conducted using standard procedures. In vitro 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay were used for the investigation of antioxidant activity of extract. Acute effects on physical and behavioral changes and mortality were monitored up to 72 h after administration of different doses of C. crispa  Chronic effects on body to organ ratio, biochemical and hematological parameters were measured after administration of rats with different doses of P. crispa extract for 30 days. Results: Alkaloids, flavonoids, phenols and tannins were the most abundant constituents found in P. crispa extract, which displayed a significant antioxidant activity measured by reducing power and DPPH assays. No physical, behavioral changes and mortality were noted following the acute treatment of rats with the extract. Similarly, no significant change in body to organ weight ratio was observed after chronic treatment. Hematological parameters including RBCs, Hb, PCV, MCV, MCH and MCHC values were unaltered while W.B.C count was elevated in P.crispa administered rats compared to control. crispa extract significantly reduced glucose, urea, creatinine, cholesterol, bilirubin, AST and ALT levels, whereas, triglycerides and total protein levels were increased in response to P.crispa treatment. Conclusions: This study demonstrates that P. crispa extract is rich in bioactive compounds and possesses significant antioxidant properties. Extract was also found to be safe and had no significant adverse effects on hematological parameters and exerted beneficial effects on biochemical parameters.

scholarly journals Current Evaluation of the Tubiflora acaulis Kuntze (Acanthaceae): Pharmacognostical, Preliminary Phytochemical Investigation and Antioxidant Activity

2021 ◽  
pp. 89-106
Author(s):  
Pravin V. Gomase ◽  
Sunil Pawar
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Diagnostic Features ◽  
Standard Drug ◽  
Physical Evaluation ◽  
Phytochemical Investigation ◽  
Plant Collection

Aim: The present work was aimed at evolving with Pharmacognostical, Preliminary Phytochemical investigation and Antioxidant activity of plant Tubiflora acaulis Kuntze (Synonym Elytraria acaulis) belong to family Acanthaceae. Study Design: The permission was taken from Maharashtra State Biodiversity Board, Nagpur for plant collection, collected and authenticated and evaluated Pharmacognostical character including morphological and microscopical as well as Physical Evaluation, phytochemical process and antioxidant activity. Place and Duration of Study: The study was carried out at PSGVPMs College of Pharmacy, Shahada, Dist- Nandurbar (MS) Affiliated to KBCNMU, Jalgaon University, Jalgaon (MS), during 2019-21. Methodology: The plant material firstly taken permission from Maharashtra State Biodiversity Board, Nagpur for plant collection, collected and authenticated and evaluated Pharmacognostical character including morphological and microscopical as well as Physical Evaluation, extraction by successive solvent method, phytochemical process and antioxidant activity of Tubiflora acaulis Kuntze (Acanthaceae). Results: The macroscopical and microscopical studies revealed useful diagnostic features. Qualitative phytochemical analyses revels that Tubiflora acaulis Kuntze leaf separately for different extract like pet. Ether, chloroform and methanol and contained the secondary metabolites. The quantitative phytochemical analysis showed the presence of secondary metabolite. The extract of Tubiflora acaulis showed the antioxidant activity by DPPH, Nitric oxide and reducing power assay. Conclusion: The findings of the current research indicated that the ethanol leaf extract of Tubiflora acaulis Kuntze (Acanthaceae) had presence of secondary metabolites and potent antioxidant properties against standard drug.

scholarly journals Proximate, Phytochemicals and Reducing Power of Leaf Extracts of Colocasia esculenta and Ipomoea batatas

2020 ◽  
pp. 1-11
Author(s):  
Achadu Abraham Eleojo ◽  
Umeh Chisom Charles ◽  
Mohammed Nimat
Keyword(s):  
Antioxidant Activity ◽  
Ipomoea Batatas ◽  
Reducing Power ◽  
Sodium Content ◽  
Colocasia Esculenta ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Calcium Iron ◽  
Analysis Determination

Background: Reports has shown that edible leaves of vegetable plants serve both nutritional and medicinal purposes, yet are poorly utilized due to inadequate enlightenment of the major populace on their compositions. Aims: The aim of this study was to determine the proximate, phytochemicals and reducing power of leaf extracts of Colocasia esculenta and Ipomoea batatas. Methodology: Washed and air dried leaf samples were milled and subjected to proximate and phytochemical analysis. Determination of calcium, iron and sodium content was by Atomic absorption spectroscopy. Reducing power was determined by the Potassium ferricyanide reducing power method. Results: Ipomoea batatas leaves was found to be higher in moisture (14.05%) and carbohydrate (29.33%) while Colocasia esculenta leaves was higher in ash (10.00%), Crude fiber (16.27%), Fat (10.17%) and protein (29.41%). A better antioxidant activity and higher levels of all phytochemicals and minerals were observed in leaves of Colocasia esculenta compared to leaves of Ipomoea batatas. Conclusion: This study suggests that both leaves are of importance to human nutrition considering the observed levels of nutrients, phytochemicals and antioxidant activity. While C. esculenta leaves should be preferred for its nutrient and antioxidant advantages, both leaves can contribute immensely to the daily nutrient requirements.

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