IN VITRO EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AN INDIAN MEDICINAL PLANT: CELASTRUS PANICULATUS WILLD

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Pradeep K Naik ◽  
Debasmita Dubey ◽  
Priya Cheruvanachari ◽  
Animesh Pattnaik ◽  
Swaraj K Babu
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Dpph Method ◽  
Celastrus Paniculatus

Aim: To evaluate the antibacterial efficacy, the presence of different phytoconstituents of the leaf extracts of Indian plant Celastrus paniculatus against 6 antibiotic-resistant bacteria along with the evaluation of their antioxidant potentiality. Methods: The leaf extracts were prepared using 4 solvents, water, methanol, n-butanol, and acetone. The antibacterial property was evaluated using agar well diffusion and micro-broth dilution method. Qualitative phytochemical analysis was performed using standard protocols for each solvent extract to check for the presence of the secondary metabolites and phytochemicals which attributes to the antibacterial effectivity of C. paniculatus. Further, the antioxidant potentiality of all the 4 leaf-solvent-extracts was estimated using the DPPH method. Results: The n-butanol leaf extract exhibited the highest antibacterial and antioxidant activity, followed by acetone, methanol extracts. The aqueous extracts exhibited negligible antibacterial activity. Conclusion: This study validated the antibacterial properties of C. paniculatus leaf extracts against both gram-positive and negative bacteria and the phytochemical analysis revealed the presence of many secondary importance which can be attributed to its therapeutic properties. Hence, this plant can be further used as a complementary or alternative choice of drug for combatting multidrug-resistant bacterial pathogens. Keywords: Antibiotic resistance; Medicinal plants; Celastrus Paniculatus; Antibacterial activity, Phytochemical Analysis; Antioxidant activity

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

2018 ◽  
Vol 11 (6) ◽  
pp. 136
Author(s):  
Chandrasekaran Swaminathan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Root Extracts ◽  
Wide Range ◽  
Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions. 

scholarly journals Evaluation of antibacterial and antioxidant potential of Euphorbia cotinifolia linn. leaf extracts

2014 ◽  
Vol 20 (1) ◽  
pp. 19-28 ◽  
Author(s):  
B. Jayalakshmi ◽  
K.A. Raveesha ◽  
K.N. Amruthesh
Keyword(s):  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Klebsiella Pneumonia ◽  
Phytochemical Analysis ◽  
Significant Activity ◽  
Leaf Extracts ◽  
Dpph Method ◽  
Ic50 Values

Antibacterial activity of aqueous and solvent extracts of E. cotinifolia leaves were tested against some human pathogenic bacteria viz. Escherichia coli, Klebsiella pneumonia, Bacillus subtilis, Bacillus cereus, Salmonella typhi, Enterobacter aerogenes and Staphylococcus aureus by agar cup diffusion and broth microdilution methods. Antioxidant properties were evaluated for different solvent extracts by diphenyl picryl hydrazyl (DPPH), nitric oxide (NO) and hydrogen peroxide methods and IC50 values were calculated and compared with the standard ascorbic acid and butylated hydroxyanisole. Among the aqueous and organic solvent extracts, methanol and ethyl acetate, showed significant activity against B. subtilis and E. aerogenes which recorded a maximum inhibition zone of 17.25 mm. Minimum inhibitory concentration of methanol and ethyl acetate extracts for different bacteria ranged from 0.3- 1.25 mg/mL. In DPPH method, IC50 values of chloroform, petroleum ether, ethyl acetate and methanol were found to be 15, 17, 18 and 19 mg/mL, respectively, lesser than the standard, ascorbic acid (25 mg/mL). Phytochemical analysis of aqueous, ethyl acetate and methanol extract showed the presence of flavonoids, terpenoids, tannins and steroids. Further work is in progress to isolate the active compound(s).

scholarly journals Mexican Sage (Salvia officinalis) Extraction Using Factorial Design and Its Effect on Chemical and Antibacterial Properties

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
L. A. Conde-Hernández ◽  
M. L. Luna-Guevara ◽  
J. J. Luna-Guevara ◽  
J. Pérez-Vázquez ◽  
R. J. Aranda-García
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Factorial Design ◽  
Antibacterial Properties ◽  
Particle Morphology ◽  
Salvia Officinalis ◽  
Resistant Bacteria ◽  
Enterobacter Agglomerans ◽  
Pareto Chart ◽  
Pharmaceutical Industries

The essential oils (EOs) extracted by hydrodistillation (HYDRO) and steam distillation (SD) from Mexican Salvia officinalis L were analyzed for yield, chemical composition (GC-MS), particle morphology (SEM), antioxidant activity (ABTS), and antibacterial activity against Enterobacter agglomerans, Citrobacter freundii, Salmonella sp, E. coli, and Pseudomonas aeruginosa. The influence of the factors (method, quantity, and sample) was evaluated using a 23 full factorial design, Pareto chart, normal probability plot, main effects, and interaction plots in variance analysis on yield and antioxidant activity. The quantity, methods, sample, and the methods × sample and methods × quantity interactions were the most significant factors on yield (%). The sample, methods, and quantity × sample interaction were significant for antioxidant activity. EO yields were between 0.35 and 1.27 (% w/w), and the highest value was obtained by the HYDRO method using 50 g of whole sage leaves. The antioxidant activity values were in the range of 2.35 to 3.44 mg Trolox equivalent/g. Camphor, limonene, camphene, and caryophyllene were the main compounds identified. Micrographs of sage leaves showed relevant changes in the structure after extraction. The antibacterial activity was confirmed with the inhibition diameter and inhibition percentage of all bacteria, and P. aeruginosa was the most resistant bacteria. Finally, S. officinalis EO potentials can be considered an alternative natural preservative for the food and pharmaceutical industries.

scholarly journals Antibacterial Activity of Guyabano, Ulasimang Bato, Sambong, and Tsaang Gubat Leaf Extracts against Common Drug-resistant Bacteria

2020 ◽  
Vol 54 (1) ◽  
Author(s):  
Cecilia C. Maramba-Lazarte ◽  
Lolita L. Cavinta ◽  
Ma. Carmelita L. Sara
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Moderate Activity ◽  
Alcoholic Extract ◽  
Drug Resistant ◽  
Leaf Extracts ◽  
E Coli

Background. Using plants as antimicrobials has long been a practice of traditional healers and validating these customs may lead to the discovery and development of useful herbal medicines. Objective. This study aimed to determine the antibacterial activity of guyabano, tsaang gubat, sambong, and ulasimang bato against common pathogens. Methods. Aqueous or alcoholic leaf extracts of the different medicinal plants were prepared. The solid agar dilution method was used to determine the MIC of guyabano, tsaang gubat, sambong, and ulasimang bato against common pathogens including Staphylococcus aureus, Echerichia coli, Streptococcus pneumoniae, Hemophilus influenzae, Pseudomonas aeruginosa, Salmonella typhi, and Shigella flexneri. Results. The alcoholic leaf extract of guyabano showed moderate activity against oxacillin-sensitive S. aureus with an MIC of 5-6.3 mg/mL. However, tsaang gubat did not exhibit any antibacterial activity for drug-resistant enteric organisms (S. typhi, S. flexneri, and E. coli) and S. aureus at a concentration of 25 mg/mL. Even at a concentration of 100mg/mL, ulasimang bato failed to show any antibacterial activity against drug-resistant S. aureus, S. pneumonia, H. influenzae, E. coli, and P. aeruginosa. Sambong alcoholic extract had some antibacterial activity against penicillin-resistant S. pneumoniae with an MIC of 12.5 mg/mL. Conclusions. Guyabano alcoholic leaf extract showed moderate antibacterial activity against oxacillin-sensitive S. aureus. Sambong alcoholic extract likewise exhibited inhibitory activity against S. pneumoniae. However, tsaang gubat and ulasimang bato aqueous extracts failed to show significant antibacterial activity for the pathogens tested.

scholarly journals Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
RIDWANTI BATUBARA ◽  
TENGKU ISMANELLY HANUM ◽  
ODING AFFANDI ◽  
HENNY SRI WAHYUNI
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Chemical Compounds ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Mature Leaves ◽  
Dpph Method ◽  
North Sumatra ◽  
Chemical Contents

Abstract. Batubara R, Hanum TI, Affandi O, Wahyuni HS. 2020. Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties. Biodiversitas 21: 4616-4622. The genus Wikstroemia is one of the genus of agarwood producing trees which has not been widely researched and published. One type of this genus is Wikstroemia tenuiramis Miq, which grows naturally in North Sumatra, the leaves are like other types of agarwood leaves can be used as raw material for agarwood leaves tea. A research has been conducted to determine the chemical contents contained in the young and mature leaves of agarwood plants species W. Tenuiramis. This research methods applied qualitative analysis using phytochemical screening, quantitative analysis to determine tannin content using titration method, antioxidant activity analysis using 2,2-diphenil-1-picrylhydrazil (DPPH) method, and compound tracing using Py-gas chromatography-mass spectrometry (Py-GC-MS) instruments. The qualitative phytochemical analysis results showed no difference in chemical compounds contained in both the young and mature leaves. Both the young and mature leaves contained flavonoids, triterpenoids, and tannins, and did not contain alkaloids, steroids and saponins. The young contained tannins of 1.079 ± 0.001% and the mature contained tannins of 4.645 ± 0.021%, not significant statistically. Py-GC-MS analysis showed the presence of various 30 bioactive compounds contained in both young and mature leaves. The analysis results also showed that both the young and mature leaves had very strong antioxidant activity. Our findings suggest that the two types of leaves have equal utility value of in term of chemical contents and antioxidant potentials.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT PROPERTIES BY DPPH RADICAL SCAVENGER ACTIVITY OF RUELLIA BRITTONIANA FLOWER

2019 ◽  
pp. 24-28
Author(s):  
NADZILA ANINDYA TEJAPUTRI ◽  
ADE ARSIANTI ◽  
FONA QORINA ◽  
QOTRUNNADA FITHROTUNNISA
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Chemical Components ◽  
Phytochemical Analysis ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Dpph Method ◽  
Tlc Analysis

Objective: The genus Ruellia has been widely used in traditional and Ayurvedic medicine as an antioxidant. This study seeks to examine the antioxidant activity of the species Ruellia brittoniana. Methods: In this study, Ruellia brittoniana flowers were acquired from Depok, West Java, Indonesia. The flowers were cleaned and ground to form a powder, then dissolved in hexane, ethanol and ethyl acetate solvents. These three extracts were then tested for phytochemicals and thin layer chromatography (TLC) analysis. Ethanol and ethyl acetate extracts were also analyzed for antioxidants using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: Phytochemical results from the three extracts proved that Ruellia brittoniana contains flavonoids, alkaloids, tannins, glycosides and triterpenes. These results are comparable to the results from TLC analysis, which showed the samples contained 4–5 chemical components. Furthermore, the best antioxidant activity resulted from the ethyl acetate extract of the Ruellia brittoniana flower with an IC50 value of 68.42 ppm. Conclusion: An ethyl acetate extract from the Ruellia brittoniana flower can be used as a natural source of additional antioxidants.

scholarly journals Total Phenolic Contents and Antioxidant Properties of Algerian Arbutus unedo L. Extracts

2020 ◽  
Vol 10 (3-s) ◽  
pp. 159-168
Author(s):  
Saliha Laouicha ◽  
Abderrahmane Senator ◽  
Abdallah Kherbache ◽  
Hamama Bouriche
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Arbutus Unedo ◽  
Phenolic Contents ◽  
Antioxidant Agents ◽  
Ic50 Values

Arbutus unedo L. is a plant widely distributed in the Mediterranean basin and North Africa, frequently used in traditional folk medicine to treat diabetes and arterial hypertension. This study aims to evaluate the phenolic composition and antioxidant activity of ethyl acetate (EA.E) and n-butanolic (But.E) extracts prepared from leaves of Arbutus unedo. Total phenolic and flavonoids contents were determined spectrophotometrically. The antioxidant activity was evaluated using DPPH•, OH•, H2O2, O•-2, ß-carotene bleaching and AAPH-induced erythrocyte oxidative hemolysis assays. The phytochemical analysis showed the presence of polyphenols and flavonoids in both extracts. The high amount was observed in EA.E which exerted the stronger antioxidant effect, with IC50 values of 3.43 μg/mL, 323.45 µg/mL, 38.40 µg/mL and 11.56 µg/mL, in DPPH•, OH•, O•-2 and H2O2 tests, respectively. Both extracts inhibited β-carotene bleaching, but EA.E is always more potent (92%) than But.E (85%). Furthermore, the EA.E showed the highest protective effect on erythrocyte hemolysis induced by AAPH, with half time hemolysis (HT50) of 122.02 min at 40 µg/mL. Taken together, this study showed that Arbutus unedo leaf extracts possess strong antioxidant potential, which may be attributed to the presence of a high amount of polyphenolic constituents. So, this plant might be exploited as a potential source of natural antioxidant agents for pharmaceutical and food applications. Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo.

scholarly journals Antioxidant and antimicrobial effects of grape pomace extracts

2019 ◽  
Vol 15 ◽  
pp. 04006 ◽  
Author(s):  
C.E. Luchian ◽  
V.V. Cotea ◽  
L. Vlase ◽  
A.M. Toiu ◽  
L.C. Colibaba ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Hplc Method ◽  
Grape Pomace ◽  
Bioactive Metabolites ◽  
Frap Assay ◽  
Antioxidant Agents ◽  
Dpph Method ◽  
Grape Varieties

The use of antioxidants and antibacterials in food industry has become increasingly necessary to ensure the high quality of food. Grape pomace is the main by-product of winemaking industry that concentrates bioactive metabolites with more studied antioxidant activity and possible antibacterial activity. The grape pomace contains fragmented skin, broken cells, pulp remains, stalks and seeds with high amount of phenolic compounds due to their poor extraction during the winemaking process. Anthocyanins, catechins, flavonol glycosides, phenolic acids, alcohols and stilbenes have been identified among the compounds present in grape pomace. In this study, antibacterial activity against different pathogens (Escherichia coli ATCC 25922, Candida albicansATCC 90028, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923) was evaluated and the relation with polyphenols content and antioxidant activity of grape pomace from selected grapes from Iaşi vineyard was studied. The grape pomace samples were obtained after the fermentation process from the 2017 harvest of Sauvignon Blanc, Traminer, Busuioacă de Bohotin, Cabernet Sauvignon, Merlot, Fetească Neagră and Fetească Regală grape varieties. The antioxidant properties were evaluated using the DPPH method, FRAP assay and Folin Ciocalteu method. The content of resveratrol was quantified using an HPLC method. Samples with antioxidant activity showed the highest phenolics content. This study reveals that grape pomace is a potential source of natural antioxidant agents. The pomace extracts were tested to establish the effects on Gram-positive and Gram-negative bacteria. The analysed samples exhibited insignificant antibacterial activity and the method requires optimization. Grape marc represents an important source of resveratrol and other bioactive compounds that could be a valuable source of antioxidants for further utilization in food and pharmaceutical industry.

scholarly journals Phenolic Compounds in Extracts of Hibiscus acetosella (Cranberry Hibiscus) and Their Antioxidant and Antibacterial Properties

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4190 ◽  
Author(s):  
Jae Il Lyu ◽  
Jaihyunk Ryu ◽  
Chang Hyun Jin ◽  
Dong-Gun Kim ◽  
Jung Min Kim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Antibacterial Properties ◽  
Activity Level ◽  
Leaf Extracts ◽  
Abts Assay ◽  
Activity Group ◽  
Zones Of Inhibition ◽  
Low Activity

Hibiscus species are rich in phenolic compounds and have been traditionally used for improving human health through their bioactive activities. The present study investigated the phenolic compounds of leaf extracts from 18 different H. acetosella accessions and evaluated their biofunctional properties, focusing on antioxidant and antibacterial activity. The most abundant phenolic compound in H. acetosella was caffeic acid, with levels ranging from 14.95 to 42.93 mg/100 g. The antioxidant activity measured by the ABTS assay allowed the accessions to be classified into two groups: a high activity group with red leaf varieties (74.71–84.02%) and a relatively low activity group with green leaf varieties (57.47–65.94%). The antioxidant activity was significantly correlated with TAC (0.933), Dp3-Sam (0.932), Dp3-Glu (0.924), and Cy3-Sam (0.913) contents (p < 0.001). The H. acetosella phenolic extracts exhibited antibacterial activity against two bacteria, with zones of inhibition between 12.00 and 13.67 mm (Staphylococcus aureus), and 10.67 and 13.33 mm (Pseudomonas aeruginosa). All accessions exhibited a basal antibacterial activity level (12 mm) against the Gram-positive S. aureus, with PI500758 and PI500764 exhibiting increased antibacterial activity (13.67 mm), but they exhibited a more dynamic antibacterial activity level against the Gram-negative P. aeruginosa.

scholarly journals Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Adelina Simamora ◽  
Adit W. Santoso ◽  
Kris H. Timotius ◽  
Ika Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Xanthine Oxidase ◽  
Metabolic Diseases ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Food Ingredient ◽  
Pbr322 Plasmid ◽  
Total Antioxidant

Premna serratifolia, commonly known as Arogo in Tentena-Sulawesi, is a popular vegetable. As a promising herbal tea and food ingredient, further investigation is required to find the best knowledge for medicinal use of P. serratifolia leaves. This research investigated the antioxidant activity of the ethanol (EEPS) and water (WEPS) extracts of P. serratifolia leaves, based on their scavenging activities on DPPH radicals and their reducing capacities (CuPRAC, total antioxidant/phosphomolybdenum, and ferric thiocyanate reducing power assays). The DNA-protecting effect by EEPS was tested using pBR322 plasmid DNA against •OH radical-induced damage. The inhibition potentials of both extracts against several enzymes related to metabolic diseases (α-glucosidase, α-amylase, xanthine oxidase, and protease) were evaluated. The phytochemical analysis was conducted by an LC-QTOF-MS/MS technique. EEPS proved to be a better antioxidant and had higher phenolic content compared to WEPS. EEPS demonstrated a protective effect on DNA with recovery percentage linearly correlated with EEPS concentrations. Strong inhibition on α-glucosidase and α-amylase was observed for EEPS; however, EEPS and WEPS showed weak inhibitions on xanthine oxidase and protease. LC-QTOF-MS/MS analysis identified seven main components in EEPS, namely scroside E, forsythoside A and forsythoside B, lavandulifolioside, diosmin, nobilin D, campneoside I, and isoacteoside. These components may be responsible for the observed enzymes inhibitions and antioxidant properties. Premna serratifolia leaves can be an appropriate choice for the development of nutraceutical and drug preparations.

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