Anti-inflammatory, antibacterial and antioxidant activity of leaf and cell cultures extracts of Randia aculeata L. and its chemical components by GC-MS

Taxus species in different parts of the world prevent the formation of hepatoprotective, anticoagulant, antiulcerogenic, anti-coagulant, antifungal and tumour cells. In this study, biological activity studies were performed on the leaves, branches and fruits of the methanol extract of Taxus baccata L. species collected from Sivaslı district of Uşak province. The methanol extract of the T.baccata species, the total amount of phenolic content of the sample was found to be 14.76 mg GAEQ/1g dry sample in the highest leaves. In T.baccata methanol extract, the maximum amount of flavonoid content was 0.468±mg QE/g dry sample. Antioxidant activity value as DPPH radical; % inhibition value was found as 93.21%. Based on the results; It can be concluded that the leaves of T.baccata have higher antioxidant activity than fruits and branches. Future studies need to explore the chemical components contained in the T.baccata species to determine and investigate in further detail.

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Design and Synthesis of Novel Anti-inflammatory/Anti-ulcer Hybrid Molecules with Antioxidant Activity

Medicinal Chemistry ◽

10.2174/1573406416666200930114340 ◽

2020 ◽

Vol 16 ◽

Author(s):

Bhim Bahadur Chaudhari ◽

Alka Bali ◽

Ajitesh Balaini

Keyword(s):

Antioxidant Activity ◽

Oral Absorption ◽

Radical Scavenging ◽

Reducing Power ◽

Antioxidant Potential ◽

Inflammatory Activity ◽

In Vivo Models ◽

Standard Drug ◽

Hybrid Molecules ◽

Anti Inflammatory

Background: NSAIDs are the most widely prescribed medications worldwide for their anti-inflammatory, antipyretic, and analgesic effects However, their chronic use can lead to several adverse drug events including GI toxicity. The selective COX-2 inhibitors developed as gastro-sparing NSAIDs also suffer from serious adverse effects which limit their efficacy. Objective: Local generation of reactive oxygen species is implicated in NSAID-mediated gastric ulceration and their combination with H2 antagonists like famotidine reduces the risk of ulcers. The objective of this work was to design and synthesize novel methanesulphonamido isoxazole derivatives by hybridizing the structural features of NSAIDs with those of antiulcer drugs (ranitidine, famotidine, etc.) to utilize a dual combination of anti-inflammatory activity and reducing (antioxidant) potential. Method: The designing process utilized three dimensional similarity studies and utilized an isoxazole core having a potential for anti-inflammatory as well as radical scavenging antioxidant activity. The compounds were assayed for their antiinflammatory activity in established in vivo models. The in vitro antioxidant activity was assessed in potassium ferricyanide reducing power (PFRAP) assay employing ascorbic acid as the standard drug. Results: Compounds (5, 6, 9 and 10) showed anti-inflammatory activity comparable to the standard drugs and were also found to be non-ulcerogenic at the test doses. Compounds 6-10 exhibited good antioxidant effect in the concentration range of 1.0-50.0 µmol/ml. The test compounds were also found to comply with the Lipinski rule suggesting good oral absorption. Conclusion: A new series of isoxazole based compounds is being reported with good anti-inflammatory activity coupled with antioxidant potential as gastro-sparing anti-inflammatory agents.

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Chemical Composition and Antibacterial and Antioxidant Activity of a Citrus Essential Oil and Its Fractions

Molecules ◽

10.3390/molecules26102888 ◽

2021 ◽

Vol 26 (10) ◽

pp. 2888

Author(s):

Carmen M. S. Ambrosio ◽

Gloria L. Diaz-Arenas ◽

Leidy P. A. Agudelo ◽

Elena Stashenko ◽

Carmen J. Contreras-Castillo ◽

...

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Biological Activities ◽

E Coli ◽

Beneficial Bacterium ◽

Minor Compounds ◽

Antibacterial And Antioxidant Activity ◽

High Relative Amount

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.

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ANTIMICROBIAL AND ANTIOXIDANT EFFECT OF NATURAL EXTRACTS FROM LEAVES, ROOT, STEM AND FLOWERS OF BACCHARIS LATIFOLIA FROM ECUADOR

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i2.37495 ◽

2020 ◽

pp. 78-84

Author(s):

FAVIAN BAYAS-MOREJON ◽

ANGELICA TIGRE ◽

RIVELINO RAMON ◽

DANILO YANEZ

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Potassium Phosphate ◽

Natural Antioxidants ◽

Butylated Hydroxytoluene ◽

Penicillin G ◽

Diffusion Method ◽

E Coli ◽

Antibacterial And Antioxidant Activity

Objective: The increase in chronic and degenerative diseases and the use of synthetic antioxidants such as (butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT)) are being restricted because they can be considered carcinogenic. Therefore, there is a growing interest in the search for natural antioxidants, especially from plants, due to their content in different bioactive compounds, such as antioxidants and antimicrobials. To evaluate the antibacterial and antioxidant activity of Baccharislatifolia extracts. Methods: For the determination of the antimicrobial activity of extracts of leaves, root, stem and flowers of Baccharislatifolia (Bl), the disk plate diffusion method was used, the strains of Listeria, Salmonella and E. coli were studied; antibiotics Penicillin G and Ciprofloxacin were the controls. For the antioxidant activity, a solution of H2O2 (Abs at 230 nm) was prepared in Potassium Phosphate Monobasic-Sodium Hydroxide buffer. Results: The antimicrobial activity against Listeria and Salmonella, showed that the extracts of leaves and flowers were more effective with inhibition zones>15 mm and>20 mm respectively. In front of E. coli, the extracts of flowers and stem were the best with zones>7.0 mm. Antibiotics studied inhibited the development of Listeria and Salmonella. However, E. coli isolates were resistant. In the antioxidant activity, the flower extract of Bl in 60 mg/ml presents a higher effect with 47.25%. Conclusion: Bl extracts from leaves and flowers were more efficient both in their antimicrobial and antioxidant capacity.

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Phytochemical Analysis of Anti-Inflammatory and Antioxidant Effects of Mahonia aquifolium Flower and Fruit Extracts

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/2879793 ◽

2018 ◽

Vol 2018 ◽

pp. 1-12 ◽

Cited By ~ 13

Author(s):

Andra-Diana Andreicut ◽

Alina Elena Pârvu ◽

Augustin Cătălin Mot ◽

Marcel Pârvu ◽

Eva Fischer Fodor ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Stress Index ◽

Tnf Alpha ◽

Phytochemical Analysis ◽

Anti Inflammatory ◽

Mahonia Aquifolium ◽

Antioxidant Effects

Oxidative stress and inflammation are interlinked processes. The aim of the study was to perform a phytochemical analysis and to evaluate the antioxidant and anti-inflammatory activities of ethanolic Mahonia aquifolium flower (MF), green fruit (MGF), and ripe fruit (MRF) extracts. Plant extract chemical composition was evaluated by HLPC. A DPPH test was used for the in vitro antioxidant activity. The in vivo antioxidant effects and the anti-inflammatory potential were tested on a rat turpentine oil-induced inflammation, by measuring serum nitric oxide (NOx) and TNF-alpha, total oxidative status (TOS), total antioxidant reactivity (TAR), oxidative stress index (OSI), 3-nitrothyrosine (3NT), malondialdehyde (MDA), and total thiols (SH). Extracts were administrated orally in three dilutions (100%, 50%, and 25%) for seven days prior to inflammation. The effects were compared to diclofenac. The HPLC polyphenol and alkaloid analysis revealed chlorogenic acid as the most abundant compound. All extracts had a good in vitro antioxidant activity, decreased NOx, TOS, and 3NT, and increased SH. TNF-alpha was reduced, and TAR increased only by MF and MGF. MDA was not influenced. Our findings suggest that M. aquifolium has anti-inflammatory and antioxidant effects that support the use in primary prevention of the inflammatory processes.

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Chemical composition, antibacterial and antioxidant activity of essential oil of Eupatorium adenophorum Spreng. from Eastern Uttar Pradesh, India

Food Bioscience ◽

10.1016/j.fbio.2014.06.001 ◽

2014 ◽

Vol 7 ◽

pp. 80-87 ◽

Cited By ~ 16

Author(s):

Abhay K. Pandey ◽

Manindra Mohan ◽

Pooja Singh ◽

Uma T. Palni ◽

N.N. Tripathi

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Uttar Pradesh ◽

Eupatorium Adenophorum ◽

Eupatorium Adenophorum Spreng ◽

Eastern Uttar Pradesh ◽

Antibacterial And Antioxidant Activity

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Anti-inflammatory and Antioxidant Activity of Ageratum conyzoides

Pharmaceutical Biology ◽

10.1076/phbi.39.5.336.5891 ◽

2001 ◽

Vol 39 (5) ◽

pp. 336-339 ◽

Cited By ~ 16

Author(s):

E.M. Galati ◽

N. Miceli ◽

M.F. Taviano ◽

R. Sanogo ◽

E. Raneri

Keyword(s):

Antioxidant Activity ◽

Ageratum Conyzoides ◽

Anti Inflammatory

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Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry ◽

10.2174/1871523020666210126144506 ◽

2021 ◽

Vol 20 ◽

Author(s):

Vinodhini Velu ◽

Swagata Banerjee ◽

Vidya Rajendran ◽

Gaurav Gupta ◽

Dinesh Kumar Chellappan ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Ethyl Acetate Extract ◽

Petroleum Ether Extract ◽

Inflammatory Activity ◽

Ether Extract ◽

Membrane Stabilization ◽

Leaf Extracts ◽

Anti Inflammatory

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

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