Interactions between auxin and quercetin during grape berry development

2016 ◽  
Vol 205 ◽  
pp. 45-51
Author(s):  
Mei Luo ◽  
Sibao Wan ◽  
Xiangyu Sun ◽  
Tingting Ma ◽  
Weidong Huang ◽  
...  
Keyword(s):  
Grape Berry ◽  
Berry Development

scholarly journals Transcriptomic Analyses of Root Restriction Effects on Phytohormone Content and Signal Transduction during Grape Berry Development and Ripening

2018 ◽  
Vol 19 (8) ◽  
pp. 2300 ◽  
Author(s):  
Feng Leng ◽  
Jinping Cao ◽  
Shiping Wang ◽  
Ling Jiang ◽  
Xian Li ◽  
...  
Keyword(s):  
Signal Transduction ◽  
High Resolution Mass Spectrometry ◽  
Transcript Abundance ◽  
Ultra Performance Liquid Chromatography ◽  
Grape Berry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Signal Transduction Pathways ◽  
Berry Development ◽  
Seedless Grape ◽  
Root Restriction

Phytohormones strongly influence growth, development and nutritional quality of agricultural products by modulating molecular and biochemical changes. The purpose of the present study was to investigate the influence of root restriction (RR) treatment on the dynamic changes of main phytohormones during the berry development and ripening of “Summer Black” early ripening seedless grape (Vitis vinifera × V. labrusca), and to analyze the changes in the biosynthesis and signal transduction pathways of phytohormones by transcriptomics. Enzyme-linked immunosorbent assay (ELISA) and Ultra Performance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS) were used to quantify the phytohormone levels, and RNA-Seq was used to analyze the transcript abundance. The results showed that 23 transcripts involved in the phytohormone biosynthesis and 34 transcripts involved in the signal transduction pathways were significantly changed by RR treatment. RR also increased abscisic acid, brassinosteroid, ethylene, jasmonic acid and salicylic acid levels, while decreasing auxin, cytokinin, and gibberellin contents. The results of the present study suggest that RR treatment can accelerate the grape ripening process, and specific candidate genes were identified for further functional analysis.

Transcriptomic Analysis of Root Restriction Effects on Phenolic Metabolites during Grape Berry Development and Ripening

2020 ◽  
Vol 68 (34) ◽  
pp. 9090-9099
Author(s):  
Feng Leng ◽  
Jinping Cao ◽  
Zhiwei Ge ◽  
Yue Wang ◽  
Chenning Zhao ◽  
...  
Keyword(s):  
Grape Berry ◽  
Transcriptomic Analysis ◽  
Berry Development ◽  
Phenolic Metabolites ◽  
Root Restriction

Grape berry development : A review

OENO One ◽  
2002 ◽  
Vol 36 (3) ◽  
pp. 109 ◽  
Author(s):  
Paraskevi Diakou-Verdin ◽  
Jean-Pierre Carde ◽  
Jean-Pierre Gaudillère ◽  
François Barrieu ◽  
Nathalie Ollat ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sugar Metabolism ◽  
Growth Period ◽  
Early Growth ◽  
Grape Berry ◽  
Special Focus ◽  
Berry Development ◽  
Growth Structure ◽  
Berry Size ◽  
Highly Sensitive

<p style="text-align: justify;">Grape berry development is reviewed with special focus on berry growth, structure, substances imported, organic acid and sugar metabolism. Berry growth is divided into two growth periods. Berry structure and ultra structure are adapted to sink function. Exocarp cells are characterized by intensive metabolic capacities, flesh cells by a storage role. Early growth is highly sensitive to internal and external parameters. Berry size is largely defined during the first growth period. After "véraison", the berry becomes a major storage sink. Many changes occur in berry metabolism and gene expression. Genomic researches are promising to elucidate the mechanisms of berry development.</p>

scholarly journals Iso-Seq allows genome-independent transcriptome profiling of grape berry development

2018 ◽  
Author(s):  
Andrea Minio ◽  
Mélanie Massonnet ◽  
Rosa Figueroa-Balderas ◽  
Amanda M. Vondras ◽  
Barbara Blanco-Ulate ◽  
...  
Keyword(s):  
Single Molecule ◽  
Transcriptional Profiling ◽  
Transcriptome Profiling ◽  
Grape Berry ◽  
Cabernet Sauvignon ◽  
Berry Development ◽  
Grape Berries ◽  
Gene Space ◽  
A Genome ◽  
Computationally Intensive

AbstractTranscriptomics has been widely applied to study grape berry development. With few exceptions, transcriptomic studies in grape are performed using the available genome sequence, PN40024, as reference. However, differences in gene content among grape accessions, which contribute to phenotypic differences among cultivars, suggest that a single reference genome does not represent the species’ entire gene space. Though whole genome assembly and annotation can reveal the relatively unique or “private” gene space of any particular cultivar, transcriptome reconstruction is a more rapid, less costly, and less computationally intensive strategy to accomplish the same goal. In this study, we used single molecule-real time sequencing (Iso-Seq) to sequence full-length cDNA and reconstruct the transcriptome of Cabernet Sauvignon berries during berry ripening. In addition, Illumina short reads from ripening berries were used to error-correct low-expression isoforms and to profile isoform expression. By comparing the annotated gene space of Cabernet Sauvignon to other grape cultivars, we demonstrate that the transcriptome reference built with Iso-Seq data represents most of the expressed genes in the grape berries and includes 1,501 cultivar-specific genes. Iso-Seq produced transcriptome profiles similar to those obtained after mapping on a complete genome reference. Together, these results justify the application of Iso-Seq to identify cultivar-specific genes and build a comprehensive reference for transcriptional profiling that circumvents the necessity of a genome reference with its associated costs and computational weight.

scholarly journals Influence of Light on Grape Berry Growth and Composition Varies during Fruit Development

1996 ◽  
Vol 121 (5) ◽  
pp. 869-874 ◽  
Author(s):  
N.K. Dokoozlian ◽  
W.M. Kliewer
Keyword(s):  
Fruit Development ◽  
Light Exposure ◽  
Grape Berry ◽  
Pinot Noir ◽  
Stage Iii ◽  
Cabernet Sauvignon ◽  
Fruit Softening ◽  
Berry Development ◽  
Development Stages ◽  
Final Sample

Potted `Cabernet Sauvignon' and `Pinot noir' grapevines (Vitis vinifera L.) grown in a sunlit phytotron were used to study the influence of cluster light exposure during various stages of fruit development on berry growth and composition. Clusters grown without light during berry development stages I and II, stage III, or stages I, II, and III, were compared to clusters exposed to light throughout fruit development (control). The temperature of light-exposed and nonexposed fruit was similar. The weights and diameters of berries grown without light during stages I and II, or stages I, II, and III, were similar and significantly lower than those of the control. Fruit softening in both cultivars, as well as the initiation of berry coloration, was delayed when berries were grown without light during stages I and II. Following fruit softening, berries grown without light during stages I, II, and III were lower in sugar than the control. On the final sample date, `Cabernet Sauvignon' berries grown without light during stages I, II, and III were higher in malate compared to the control. `Pinot noir' berries grown without light during stages I and II, or stages I, II, and III, were lower in malate before fruit softening, and higher in malate following fruit softening, than the control. Control berries had greater skin anthocyanins and phenolics compared to the remaining treatments. Berries grown without light during stages I and II, or stage III, were greater in anthocyanins and phenolics than fruit grown without light during stages I, II, and III. Light had no effect on fruit tartrate concentration or juice pH. Light had its greatest impact on fruit development during the initial stages of berry growth. Berry growth was reduced and ripening delayed when fruit were grown without light during stages I and II. Normal fruit development was not fully restored when these fruit were exposed to light during stage III.

scholarly journals Sucrose Breakdown and Synthesis in the Ripening Grape Berry

1967 ◽  
Vol 20 (2) ◽  
pp. 465 ◽  
Author(s):  
PJ Hardy
Keyword(s):  
Sugar Content ◽  
Grape Berry ◽  
The Other ◽  
Berry Development ◽  
Stages Of Development ◽  
Early Stages

Sucrose is the major translocated sugar in the grapevine (Swanson and EIShishiny 1958), but in the berry glucose and fructose together make up the bulk of the sugar content at all stages of development (Kliewer 1965a). The inversion of sucrose to glucose and fructose in ripening berries is suggested by the presence of invertase (Arnold 1965) and by the occurrence of approximately equal amounts of glucose and fructose (Kliewer 1965a). During the early stages of berry development, glucose and fructose concentrations are low, but at the onset of ripening there begins a phase of rapid glucose and fructose accumulation (Kliewer 1965a). Besides sugar, the other major soluble constituents of grapes are malate and tartrate. The concentrations of both substances increase rapidly in the immature berry, but when ripening commences sharp decreases occur, particularly in the concentration of malate (Kliewer 1965b). Some workers believe that malate and tartrate are translocated from the leaves (Amerine 1956; Peynaud and Maurie 1958) but there is evidence that some at least of the malate and tartrate is synthesized in the berry (Hale 1962). The mechanisms of the syntheses of malate and tartrate in the berry are not known.

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