Germplasm conservation, molecular identity and morphological characterization of persimmon (Diospyros kaki Thunb.) in the NFGP of China

In this study, carried out in University of Cukurova, Faculty of Agriculture, Department of Horticulture, morphological and molecular characterization of 20 persimmon accessions were performed. To determine morphological characterization, plant development stage, growth, and brunch structure, characteristics of leaf, fruit and seed were investigated. Data obtained by morphological characterization were subjected to similarity coefficient, principal components and cluster analyses to demonstrate the overall phenotypic relationships among these genotypes. The first 3 components contained 55.11% of the total multivariate variation. According to a cluster analysis, the similarity index of the population consists of 20 persimmon genotypes ranged between 6.01% and 67.47%. According to molecular characterization by full name (RAPD) markers, high level of genetic polymorphism was determined in persimmons. Although the high polymorphism, close genetic relationships have been determined among some genotypes. According to the RAPD markers, 82% similarity level was identified between ‘Hana Fuyu’ (Fr) and ‘Hachiya’ (Fr). Also ‘Fuji’ variety has shown a similarity of 80% between these genotypes. In addition, ‘Kaki Tipo’ (Fr), 07 TH 05, TH 01 07 TH 06 and 33 genotypes were clustered in the same group, and similarity level has been determined to be above 65%.

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MORPHOLOGICAL CHARACTERIZATION OF BROWN SARSON (BRASSICA RAPA L. ) GENOTYPES FOR DUS TRAITS

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.no1.041 ◽

2021 ◽

Vol 21 (no 1) ◽

Author(s):

Tahmeena Bano ◽

Shahina A. Nagoo ◽

M. Asif Iqbal Qureshi ◽

M. Altaf Wani ◽

Sanjay Kumar ◽

...

Keyword(s):

Brassica Rapa ◽

Edible Oils ◽

Common Knowledge ◽

Block Design ◽

Plant Genetic Resources ◽

Germplasm Conservation ◽

Morphological Characterization ◽

Breeding Programmes ◽

Oilseed Breeding

Rapeseed and mustard , the two important oleriferous Brassicae grown in India play a magnanimous role in the national market of edible oils and meals. But the production of the crop is low due to a narrow genetic base. Much effort has been put to diversifying the oilseed breeding. The morphological characterization is the first step towards diversity analysis and conservation of plant genetic resources. A major challenge facing those involved in the testing of new plant varieties for Distinctness, Uniformity, and Stability (DUS) is the need to compare them against all those of ‘common knowledge. Protection of Plant varieties and Farmer’s Right Act insists on Distinctness, Uniformity, and Stability (DUS) characterization of new varieties and recommends the registration of varieties for any one specific novel character. On this backdrop, the present experiment was carried out to evaluate 54 Brassica rapa L. genotypes for DUS characterization under randomized complete block design ( RCBD) with three replication at the experimental farm of GPB, FoA SKUAST-K, Wadura during rabi 2019-2020. The results of the study revealed wide variation in various traits among different Brassica rapa genotypes L. The assessment of 54 genotypes for 09 traits revealed that all the traits were informative with respect to trait expression cum characterization. The different characters studied were having great impact for selection of superior genotypes for further breeding programmes. Hence, the diversity observed among the genotypes could be utilized for cultivar improvement and germplasm conservation programs aimed at improving productivity in Brassica rapaL

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Morphological characterization of the IVIA persimmon (Diospyros kaki Thunb.) germplasm collection by multivariate analysis

Genetic Resources and Crop Evolution ◽

10.1007/s10722-012-9828-4 ◽

2012 ◽

Vol 60 (1) ◽

pp. 233-241 ◽

Cited By ~ 9

Author(s):

J. Martínez-Calvo ◽

M. Naval ◽

E. Zuriaga ◽

G. Llácer ◽

M. L. Badenes

Keyword(s):

Multivariate Analysis ◽

Germplasm Collection ◽

Morphological Characterization ◽

Diospyros Kaki

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Morphological Characterization of Mycobacteriophage R1

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051281 ◽

1974 ◽

Vol 32 ◽

pp. 254-255

Author(s):

B. L. Soloff ◽

T. A. Rado

Keyword(s):

Carbon Source ◽

Stationary Phase ◽

Growth Phase ◽

Minimal Medium ◽

Morphological Characterization ◽

Logarithmic Growth Phase ◽

Complete Lysis ◽

Lysogenic Culture ◽

Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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Morphological Characterization of Rhinacanthus Species of Sri Lanka

Planta Medica ◽

10.1055/s-0030-1251769 ◽

2010 ◽

Vol 76 (05) ◽

Author(s):

APPR Amarasinghe ◽

RP Karunagoda ◽

DSA Wijesundara

Keyword(s):

Sri Lanka ◽

Morphological Characterization

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Density and morphological characterization of bacteria in integrated and exclusive production systems

Scientific Electronic Archives ◽

10.36560/13920201194 ◽

2020 ◽

Vol 13 (11) ◽

pp. 1

Author(s):

A. R. B. Zanco ◽

A. Ferreira ◽

G. C. M. Berber ◽

E. N. Gonzaga ◽

D. C. C. Sabino

Keyword(s):

Bacterial Community ◽

Rainy Season ◽

Production Systems ◽

Cropping System ◽

Morphological Characterization ◽

Native Forest ◽

Exclusive Production ◽

Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season

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Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

Infectious Disorders - Drug Targets ◽

10.2174/1871526519666181226123541 ◽

2020 ◽

Vol 20 (4) ◽

pp. 448-454

Author(s):

Rahmita Burhamzah ◽

Gemini Alam ◽

Herlina Rante

Keyword(s):

Molecular Characterization ◽

Endophytic Fungi ◽

Morphological Characterization ◽

Test Method ◽

Analysis Method ◽

Nitrogen Base ◽

Rdna Genes ◽

Antibacterial Screening ◽

Planting Method

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

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Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

Membranes ◽

10.3390/membranes11050303 ◽

2021 ◽

Vol 11 (5) ◽

pp. 303

Author(s):

Rokayya Sami ◽

Schahrazad Soltane ◽

Mahmoud Helal

Keyword(s):

Ph Value ◽

Silica Nanoparticle ◽

Morphological Characterization ◽

Aerobic Bacteria ◽

Nano Materials ◽

Elongation At Break ◽

Stable Suspension ◽

Average Size ◽

Material Ductility

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.

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