Antifungal activity of nanoemulsion from Cleome viscosa essential oil against food-borne pathogenic Candida albicans

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

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Mexican oregano (Lippia graveolens) essential oil-in-water emulsions: impact of emulsifier type on the antifungal activity of Candida albicans

Food Science and Biotechnology ◽

10.1007/s10068-018-0499-6 ◽

2018 ◽

Vol 28 (2) ◽

pp. 441-448 ◽

Cited By ~ 3

Author(s):

S. E. Herrera-Rodríguez ◽

R. J. López-Rivera ◽

E. García-Márquez ◽

M. Estarrón-Espinosa ◽

H. Espinosa-Andrews

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Oil In Water

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Antifungal Activity of Rosmarinus Officinalis Essential Oil and Nystatin on Store Isolate of Candida species from HIV/AIDS Patients with Oral Candidiasis

Berkala Ilmu Kesehatan Kulit dan Kelamin ◽

10.20473/bikk.v32.3.2020.167-173 ◽

2020 ◽

Vol 32 (3) ◽

pp. 167

Author(s):

Bernadya Yogatri Anjuwita ◽

Iskandar Zulkarnain ◽

Muhammad Yulianto Listiawan ◽

Evy Ervianti ◽

Rahmadewi Rahmadewi ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Candida Species ◽

Oral Candidiasis ◽

Rosmarinus Officinalis ◽

Minimum Fungicidal Concentration ◽

Microdilution Method ◽

Rosemary Essential Oil

Background: Oral candidiasis is caused by the mycotic activity of Candida albicans present in the oral cavity, and it is one of the most common opportunistic infections found in patients with Human Immunodeficiency Virus (HIV)/acquired immune deficiency syndrome (AIDS). The growing resistance and side effects to common antifungal drugs have promoted herbal essential oils as antifungal agents in recent years. In this study, essential oils (EO) of Rosmarinus officinalis (Lamiaceae) were examined for in vitro antifungal activity against Candida species. Purpose: To evaluate the antifungal activity of essential oils of Rosmarinus officinalis (Lamiaceae) and nystatin using the microdilution technique by determining the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of Candida spesies. Methods: This was an experimental laboratory study with a post-test-only design conducted in Dr. Soetomo General Academic Teaching Hospital, Surabaya. Forty isolates consisted of twenty isolates of Candida albicans and twenty isolates of Candida non-albicans were collected. The isolates were tested for antifungal activity using the microdilution on 96-well plates. Result: There was a significant difference from the results of the MIC concentration of rosemary essential 100% to 6.25% microdilution method between nystatin and rosemary essential oil (p < 0.05). Conclusion: The antifungal activity of rosemary essential oil was better than nystatin that the lowest MIC value, which was 6.25%, has been obtained the microdilution method. The minimum fungicidal concentration of rosemary essential oil was 25%, while the minimum fungicidal concentration nystatin was higher than 100%.

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Antifungal Activity of Artemisia Nilagirica Essential Oil from the Western Ghats Nilgiris against Food Borne Fungi

Journal of Applied Microbiology and Biochemistry ◽

10.21767/2576-1412.100022 ◽

2018 ◽

Vol 02 (02) ◽

Author(s):

Kishmu Lingan

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Western Ghats ◽

Food Borne ◽

Artemisia Nilagirica ◽

The Western Ghats

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Antifungal activity of Rosmarinus officinalis Linn. essential oil against Candida albicans, Candida dubliniensis, Candida parapsilosis and Candida krusei

Revista Pan-Amazônica de Saúde ◽

10.5123/s2176-62232014000100007 ◽

2014 ◽

Vol 5 (1) ◽

pp. 61-66 ◽

Cited By ~ 6

Author(s):

Lurdete Maria Rocha Gauch ◽

Simone Soares Pedrosa ◽

Renata Antunes Esteves ◽

Fabíola Silveira-Gomes ◽

Ely Simone Cajueiro Gurgel ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Candida Parapsilosis ◽

Rosmarinus Officinalis ◽

Candida Krusei ◽

Candida Dubliniensis

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Antifungal activity of onion (Allium cepa L.) essential oil on Candida albicans

Ilmu Gizi Indonesia ◽

10.35842/ilgi.v3i1.128 ◽

2019 ◽

Vol 3 (1) ◽

pp. 59

Author(s):

Miftahur Rahmi ◽

Felicia Aileen Agustia

Keyword(s):

Candida Albicans ◽

Minimum Inhibitory Concentration ◽

Antifungal Activity ◽

Essential Oil ◽

Allium Cepa ◽

Inhibitory Concentration ◽

Fungal Growth ◽

Diffusion Method ◽

Minimum Concentration ◽

Allium Cepa L

Background:Onions (Allium cepa L.) contain essential oil that have sulfur compounds called thiosulfinates or isothiocyanates which are known to have antibacterial and antifungal effects. Minimum Inhibition Concentration (MIC) test is needed to determine the minimum concentration of onion essential oil which can inhibit or kill fungus test. Objectives: To determine the antifungal activity of essential oil in onion (Allium cepa L.) and to find out the Minimum Inhibitory Concentration (MIC) on Candida albicans. Methods:This research was an experimental study with disc diffusion method (Kirby Bauer) using Sabourond Dextrose Agar (SDA) media. Results: The results were obtained at concentrations of 20% (C1); 10% (C2); 5% (C3); 2.5% (C4); 1.25% (C5); 0.625% (C6); and 0.312% (C7) provided inhibition with an average diameter of 13.14 mm; 9.33 mm; 8.49 mm; 6.90 mm; 0 mm; 0 mm; and 0 mm respectively. Conclusion: Essential oil of onion (Allium cepa L.) could inhibit fungal growth of Candida albicans and Minimum Inhibitory Concentration (MIC) of essential oil onions (Allium cepa L.) indicated on concentration of 2.5% with a flat diameter average of 6.90 mm.

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Comparison of In Vitro Testing Antifungal Activity between Rosemary Essentials Oil and Fluconazol on Candida species Isolate from HIV/AIDS Patients with Candidiasis Oral

Berkala Ilmu Kesehatan Kulit dan Kelamin ◽

10.20473/bikk.v32.3.2020.182-188 ◽

2020 ◽

Vol 32 (3) ◽

pp. 182

Author(s):

Putri Intan Primasari ◽

Dwi Murtiastutik ◽

Pepy Dwi Endraswari ◽

Cita Rosita Sigit Prakoeswa ◽

Evy Ervianti

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Inhibition Zone ◽

Aids Patients ◽

Candida Sp ◽

Significant Difference ◽

Rosemary Essential Oil ◽

Hiv Aids

Background: The most common opportunistic infection obtained in patients with Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS) is Oral Candidiasis (OC). Currently widely reported about increased fluconazole resistance, as a therapy that is often used in patients with HIV/AIDS with OC. For this reason, effective antifungal agents are needed as alternative therapies. Several studies have reported some antifungal activity from rosemary essential oils. Purpose: The aim of this study is to evaluate the antifungal activity between rosemary essential oils and fluconazole against the isolates used by Candida sp. taken from HIV / AIDS patients with OC. Methods: This research was an experimental laboratory study with a post-test only design of 40 Candida sp. isolates from HIV / AIDS patients with OC in the Outpatient Unit and Inpatient Installation of the Infectious Disease Intermediate Care Unit (UPIPI) Dr. Soetomo General Academic Teaching Hospital, Surabaya. Examination of antifungal activity using conventional test methods with disk diffusion. Result: Disk diffusion test result revealed average inhibition zone of rosemary essential oil in Candida albicans 1,75±3,64 mm and Candida non-albicans 1,5±3,08 mm, while the average inhibition zone of fluconazole in Candida albicans 17,9±8,62 mm and Candida non-albicans 4,85±7,9 mm. There was significant difference (p <0.001) between the inhibition zone formed in Candida albicans by fluconazole and rosemary essential oil, but no significant difference of inhibition zone formed in Candida non-albicans. Conclusion: Antifungal activity fluconazole has greater than Rosemary essential oils in Candida albicans.

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Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg

PeerJ ◽

10.7717/peerj.5476 ◽

2018 ◽

Vol 6 ◽

pp. e5476 ◽

Cited By ~ 8

Author(s):

Delmacia G. de Macêdo ◽

Marta Maria A. Souza ◽

Maria Flaviana B. Morais-Braga ◽

Henrique Douglas M. Coutinho ◽

Antonia Thassya L. dos Santos ◽

...

Keyword(s):

Candida Albicans ◽

Chemical Composition ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Fungal Infections ◽

Fungal Resistance ◽

Chemical Profile ◽

Minimum Fungicidal Concentration ◽

Broth Microdilution Method

Medicinal plants play a crucial role in the search for components that are capable of neutralizing the multiple mechanisms of fungal resistance. Psidium salutare (Kunth) O. Berg is a plant native to Brazil used as both food and traditional medicine to treat diseases and symptoms such as stomach ache and diarrhea, whose symptoms could be related to fungal infections from the genus Candida. The objective of this study was to investigate the influence of seasonal variability on the chemical composition of the Psidium salutare essential oil, its antifungal potential and its effect on the Candida albicans morphogenesis. The essential oils were collected in three different seasonal collection periods and isolated by the hydrodistillation process in a modified Clevenger apparatus with identification of the chemical composition determined by gas chromatography coupled to mass spectrometry (GC/MS). The antifungal assays were performed against Candida strains through the broth microdilution method to determine the minimum fungicidal concentration (MFC). Fungal growth was assessed by optical density reading and the Candida albicans dimorphic effect was evaluated by optical microscopy in microculture chambers. The chemical profile of the essential oils identified 40 substances in the different collection periods with γ-terpinene being the predominant constituent. The antifungal activity revealed an action against the C. albicans, C. krusei and C. tropicalis strains with an IC50 ranging from 345.5 to 2,754.2 µg/mL and a MFC higher than 1,024 µg/mL. When combined with essential oils at sub-inhibitory concentrations (MIC/16), fluconazole had its potentiated effect, i.e. a synergistic effect was observed in the combination of fluconazole with P.salutare oil against all Candida strains; however, for C. albicans, its effect was reinforced by the natural product in all the collection periods. The results show that the Psidium salutare oil affected the dimorphic transition capacity, significantly reducing the formation of hyphae and pseudohyphae in increasing concentrations. The results show that P. salutare oil exhibits a significant antifungal activity against three Candida species and that it can act in synergy with fluconazole. These results support the notion that this plant may have a potential use in pharmaceutical and preservative products.

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THE ESSENTIAL OIL CONTENTS OF JERINGAU (Acorus calamus L.) RHIZOMES AND THEIR ANTIFUNGAL ACTIVITY AGAINST Candida albicans

Journal of Health Sciences and Medicine ◽

10.24843/jhsm.2017.v01.i01.p09 ◽

2017 ◽

Vol 1 (1) ◽

pp. 33 ◽

Cited By ~ 2

Author(s):

Wiwik Susanah Rita ◽

Retno Kawuri ◽

I Made Dira Swantara

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Steam Distillation ◽

Inhibitory Concentration ◽

Diffusion Method ◽

Acorus Calamus ◽

Oil Concentration ◽

Trans Methyl

Acorus calamus L. rhizome was trusted having antibacterial activity. This study aimed to identify the compounds in the Acorus Calamus L. rhizomes essential oils and to recognize the antifungal activity of the oils against Candida albicans. The extraction of essential oils from rhizome was carried out by steam distillation technique. Identification of compounds in the oils was conducted by Gas Chromatography- Mass Spectroscopy (GCMS), while the antifungal test against Candida albicans was done by well diffusion method. Extraction of 10 kg of rhizomes produced 16.53 mL essential oil with a yield of 0.1653% (? = 1.066), the oil was brownish yellow and very flavorful. GC-MS analysis showed that the essential oil contained 11 compounds, they are (E)-3,7 dimethyl-1,3,6-Octatriene (trans-?-Ocimene) (3,73%), linalool (1,07%), ?-elemene (1,15%), trans methyl isoeugenol (7,68%), shyobunon (15,74%), bicyclogermakren (0,93%), dehidroxy-isocalamendiol (2,61%), ?-calacorene (3,34%), euasarone (26,84), cis-asarone (18,62%); dan trans- asarone (18,29%). Antifungal activity test showed that the growth and biomass inhibition of C. albicans increased with the increase of the oil concentration. Minimum Inhibitory Concentration (MIC) of essential oil toward C. albicans was 1% with the inhibition of 7.83 mm.

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Antibacterial and Antifungal Activity Analysis of Essential Oil of Pogostemon cablin (Blanco) Benth

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v30i1-2.28446 ◽

2016 ◽

Vol 30 (1-2) ◽

pp. 7-10 ◽

Cited By ~ 5

Author(s):

Puspa Das ◽

Shoma Dutta ◽

Jaripa Begum ◽

Md Nural Anwar

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Bacillus Cereus ◽

Pathogenic Bacteria ◽

Pathogenic Fungi ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Pogostemon Cablin ◽

Antibacterial And Antifungal

The essential oil of Pogostemon cablin (Blanco) Benth, also known as Patchouli oil was subjected for its antimicrobial investigation against a panel of ten human pathogenic bacteria and six human pathogenic fungi by Agar well diffusion method and Macrobroth dilution technique using Ampicillin (20ìg/well) and Nystatin (20ìg/well) as control. Antibacterial activity revealed that, the essential oil was more active against Gram positive bacteria than Gram negative bacteria. The largest zone of inhibition was 35 mm (against Bacillus cereus) with 20 ìl of oil. Ditermination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) showed that, Bacillus cereus exhibited the lowest MIC (250 ìg/ml) and MBC (750 ìg/ml). The oil showed moderate antifungal activity against all tested organisms. Candida albicans showed greater zone of inhibition (16 mm) than Saccharomyces cerevisiae (14 mm) with 20 ìl and Candida albicans showed lowest MIC and MFC (both were 750 ìg/ml). The zone of inhibition was 25 mm for each filamentous fungal strain with 20 ìl, except for Rhizopus oligosporus (15 mm) and the lowest MIC (250 ìg/ml) and MFC (500 ìg/ml) were reported for Aspergillus fumigatus.Bangladesh J Microbiol, Volume 30, Number 1-2,June-Dec 2013, pp 7-10

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