Prebiotics and iron bioavailability? Unveiling the hidden association - A review

Pulse crop seed coats are a sustainable source of antioxidant polyphenols, but are typically treated as low-value products, partly because some polyphenols reduce iron bioavailability in humans. This study correlates antioxidant/iron chelation capabilities of diverse seed coat types from five major pulse crops (common bean, lentil, pea, chickpea and faba bean) with polyphenol composition using mass spectrometry. Untargeted metabolomics was used to identify key differences and a hierarchical analysis revealed that common beans had the most diverse polyphenol profiles among these pulse crops. The highest antioxidant capacities were found in seed coats of black bean and all tannin lentils, followed by maple pea, however, tannin lentils showed much lower iron chelation among these seed coats. Thus, tannin lentils are more desirable sources as natural antioxidants in food applications, whereas black bean and maple pea are more suitable sources for industrial applications. Regardless of pulse crop, proanthocyanidins were primary contributors to antioxidant capacity, and to a lesser extent, anthocyanins and flavan-3-ols, whereas glycosylated flavonols contributed minimally. Higher iron chelation was primarily attributed to proanthocyanidin composition, and also myricetin 3-O-glucoside in black bean. Seed coats having proanthocyanidins that are primarily prodelphinidins show higher iron chelation compared with those containing procyanidins and/or propelargonidins.

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Iron Bioavailability from Diets Consumed by Different Socioeconomic Strata of the Venezuelan Population

Journal of Nutrition ◽

10.1093/jn/125.7.1860 ◽

1995 ◽

Vol 125 (7) ◽

pp. 1860-1868 ◽

Cited By ~ 30

Author(s):

Peter G. Taylor ◽

Hernán Méndez-Castellanos ◽

Carlos Martínez-Torres ◽

Werner Jaffe ◽

Mercedes López de Blanco ◽

...

Keyword(s):

Iron Bioavailability ◽

Socioeconomic Strata ◽

Venezuelan Population

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Expression of a Truncated Yeast Ccc1 Vacuolar Transporter Increases the Accumulation of Endogenous Iron

Genes ◽

10.3390/genes12081120 ◽

2021 ◽

Vol 12 (8) ◽

pp. 1120

Author(s):

Raquel Sorribes-Dauden ◽

María Teresa Martínez-Pastor ◽

Sergi Puig

Keyword(s):

Iron Homeostasis ◽

Sufficient Conditions ◽

Constitutive Expression ◽

Iron Bioavailability ◽

Full Length ◽

Yeast Cells ◽

Deficiency Anemia ◽

Functional Protein ◽

Amino Terminal ◽

In Cells

Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox cofactor in multiple metabolic processes. Iron bioavailability is highly restricted due to the low solubility of its oxidized form, frequently leading to iron deficiency anemia. The baker’s yeast Saccharomyces cerevisiae is used as a model organism for iron homeostasis studies, but also as a food supplement and fermentative microorganism in the food industry. Yeast cells use the vacuolar Ccc1 transporter to detoxify and store excess iron in the vacuoles. Here, we modulate CCC1 expression and properties to increase iron extraction from the environment. We show that constitutive expression of full-length CCC1 is toxic, whereas deletion of its cytosolic amino-terminal (Nt) domain (NtDCCC1) rescues this phenotype. Toxicity is exacerbated in cells lacking AFT1 transcription factor. Further characterization of NtDCcc1 protein suggests that it is a partially functional protein. Western blot analyses indicate that deletion of Ccc1 Nt domain does not significantly alter GFP-Ccc1 protein stability. A functional full-length GFP-Ccc1 protein localized to particular regions of the vacuolar membrane, whereas GFP-NtDCcc1 protein was evenly distributed throughout this endogenous membrane. Interestingly, expression of NtDCCC1 increased the accumulation of endogenous iron in cells cultivated under iron-sufficient conditions, a strategy that could be used to extract iron from media that are not rich in iron.

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Petrobactin, a siderophore produced by Alteromonas, mediates community iron acquisition in the global ocean

The ISME Journal ◽

10.1038/s41396-021-01065-y ◽

2021 ◽

Author(s):

Lauren E. Manck ◽

Jiwoon Park ◽

Benjamin J. Tully ◽

Alfonso M. Poire ◽

Randelle M. Bundy ◽

...

Keyword(s):

Biosynthetic Pathway ◽

Iron Acquisition ◽

Biogeochemical Cycling ◽

Iron Bioavailability ◽

Siderophore Production ◽

Global Ocean ◽

The North ◽

The North Pacific ◽

Alteromonas Macleodii

AbstractIt is now widely accepted that siderophores play a role in marine iron biogeochemical cycling. However, the mechanisms by which siderophores affect the availability of iron from specific sources and the resulting significance of these processes on iron biogeochemical cycling as a whole have remained largely untested. In this study, we develop a model system for testing the effects of siderophore production on iron bioavailability using the marine copiotroph Alteromonas macleodii ATCC 27126. Through the generation of the knockout cell line ΔasbB::kmr, which lacks siderophore biosynthetic capabilities, we demonstrate that the production of the siderophore petrobactin enables the acquisition of iron from mineral sources and weaker iron-ligand complexes. Notably, the utilization of lithogenic iron, such as that from atmospheric dust, indicates a significant role for siderophores in the incorporation of new iron into marine systems. We have also detected petrobactin, a photoreactive siderophore, directly from seawater in the mid-latitudes of the North Pacific and have identified the biosynthetic pathway for petrobactin in bacterial metagenome-assembled genomes widely distributed across the global ocean. Together, these results improve our mechanistic understanding of the role of siderophore production in iron biogeochemical cycling in the marine environment wherein iron speciation, bioavailability, and residence time can be directly influenced by microbial activities.

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Improving iron bioavailability and nutritional value of maize (Zea maysL.) in sulfur-treated calcareous soil

Archives of Agronomy and Soil Science ◽

10.1080/03650340.2016.1266484 ◽

2016 ◽

Vol 63 (9) ◽

pp. 1255-1266 ◽

Cited By ~ 3

Author(s):

Pia Muhammad Adnan Ramzani ◽

Muhammad Khalid ◽

Shazia Anjum ◽

Waqas-ud-Din Khan ◽

Muhammad Iqbal ◽

...

Keyword(s):

Calcareous Soil ◽

Nutritional Value ◽

Iron Bioavailability

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Comparative study of not from concentrate and reconstituted from concentrate of pomegranate juices on nutritional and sensory profile

Food Science and Technology International ◽

10.1177/10820132211003707 ◽

2021 ◽

pp. 108201322110037

Author(s):

G Brunda ◽

Urs Kavyashree ◽

Shilpa S Shetty ◽

Kirti Sharma

Keyword(s):

Antioxidant Activity ◽

Comparative Study ◽

Essential Elements ◽

Iron Bioavailability ◽

Sensory Characteristics ◽

Sensory Profile ◽

Pomegranate Juice ◽

Anthocyanin Content ◽

Total Anthocyanin ◽

Hepg2 Cell Lines

Pomegranate juice is popular due to its unique health benefits, sensory characteristics and also a good source of bioactive compounds. Comparative study on processing effect of Not from Concentrate (NFC) and Reconstituted from concentrate (RFC) pomegranate juice on the nutritional and sensory characteristics of ‘Ganesh’ variant was conducted. Results showed that not much differences observed in parameters like pH, acidity, essential elements, protein, total sugars and polyphenol content between NFC and RFC. As per the study NFC had a better antioxidant activity with intracellular ROS inhibition of 11% higher with significant ( p < 0.05) than RFC in HepG2 cell lines. Total anthocyanin content was significantly different ( p < 0.05) in NFC (428.05 mg/l) compared to 326.74 mg/l in RFC expressed as cyanidin-3-glucoside. Iron uptake was 40 units (µg/mg protein) higher in NFC than RFC ( p < 0.05) in HepG2 cells. Sensory flavor profile showed NFC having significant differences with respect to characteristic pomegranate freshness, fruitiness, sweetness and astringency mouthfeel. RFC had higher sweetish and cooked flavor with additional vegetable like notes of beet and carrot. Based on the data better antioxidant activity, iron bioavailability, anthocyanin content and sensory attributes were captured in pomegranate NFC juices over RFC juices.

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Hemicellulose Does Not Affect Iron Bioavailability in Chicks

Journal of Nutrition ◽

10.1093/jn/126.1.308 ◽

1996 ◽

Vol 126 (1) ◽

pp. 308-316 ◽

Cited By ~ 10

Author(s):

Alyce D. Fly ◽

Gail L. Czarnecki-Maulden ◽

George C. Fahey ◽

Evan C. Titgemeyer

Keyword(s):

Iron Bioavailability

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Iron stable isotopes track pelagic iron cycling during a subtropical phytoplankton bloom

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1421576112 ◽

2014 ◽

Vol 112 (1) ◽

pp. E15-E20 ◽

Cited By ~ 33

Author(s):

Michael J. Ellwood ◽

David A. Hutchins ◽

Maeve C. Lohan ◽

Angela Milne ◽

Philipp Nasemann ◽

...

Keyword(s):

Stable Isotopes ◽

Mixed Layer ◽

Phytoplankton Bloom ◽

Iron Bioavailability ◽

Global Ocean ◽

Minor Role ◽

Surface Mixed Layer ◽

Spring Phytoplankton Bloom ◽

Dissolved Iron ◽

Particulate Iron

The supply and bioavailability of dissolved iron sets the magnitude of surface productivity for ∼40% of the global ocean. The redox state, organic complexation, and phase (dissolved versus particulate) of iron are key determinants of iron bioavailability in the marine realm, although the mechanisms facilitating exchange between iron species (inorganic and organic) and phases are poorly constrained. Here we use the isotope fingerprint of dissolved and particulate iron to reveal distinct isotopic signatures for biological uptake of iron during a GEOTRACES process study focused on a temperate spring phytoplankton bloom in subtropical waters. At the onset of the bloom, dissolved iron within the mixed layer was isotopically light relative to particulate iron. The isotopically light dissolved iron pool likely results from the reduction of particulate iron via photochemical and (to a lesser extent) biologically mediated reduction processes. As the bloom develops, dissolved iron within the surface mixed layer becomes isotopically heavy, reflecting the dominance of biological processing of iron as it is removed from solution, while scavenging appears to play a minor role. As stable isotopes have shown for major elements like nitrogen, iron isotopes offer a new window into our understanding of the biogeochemical cycling of iron, thereby allowing us to disentangle a suite of concurrent biotic and abiotic transformations of this key biolimiting element.

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An intensified training schedule in recreational male runners is associated with increases in erythropoiesis and inflammation and a net reduction in plasma hepcidin

American Journal of Clinical Nutrition ◽

10.1093/ajcn/nqy247 ◽

2018 ◽

Vol 108 (6) ◽

pp. 1324-1333 ◽

Cited By ~ 6

Author(s):

Diego Moretti ◽

Samuel Mettler ◽

Christophe Zeder ◽

Carsten Lundby ◽

Anneke Geurts-Moetspot ◽

...

Keyword(s):

Exercise Training ◽

Iron Absorption ◽

Iron Status ◽

Geometric Mean ◽

Iron Bioavailability ◽

Oral Iron ◽

Low Grade ◽

Hemoglobin Mass ◽

Low Grade Inflammation ◽

Iron Utilization

ABSTRACT Background Iron status is a determinant of physical performance, but training may induce both low-grade inflammation and erythropoiesis, exerting opposing influences on hepcidin and iron metabolism. To our knowledge, the combined effects on iron absorption and utilization during training have not been examined directly in humans. Objective We hypothesized that 3 wk of exercise training in recreational male runners would decrease oral iron bioavailability by increasing inflammation and hepcidin concentrations. Design In a prospective intervention, nonanemic, iron-sufficient men (n = 10) completed a 34-d study consisting of a 16-d control phase and a 22-d exercise-training phase of 8 km running every second day. We measured oral iron absorption and erythroid iron utilization using oral 57Fe and intravenous 58Fe tracers administered before and during training. We measured hemoglobin mass (mHb) and total red blood cell volume (RCV) by carbon monoxide rebreathing. Iron status, interleukin-6 (IL-6), plasma hepcidin (PHep), erythropoietin (EPO), and erythroferrone were measured before, during, and after training. Results Exercise training induced inflammation, as indicated by an increased mean ± SD IL-6 (0.87 ± 1.1 to 5.17 ± 2.2 pg/mL; P < 0.01), while also enhancing erythropoiesis, as indicated by an increase in mean EPO (0.66 ± 0.42 to 2.06 ± 1.6 IU/L), mHb (10.5 ± 1.6 to 10.8 ± 1.8 g/kg body weight), and mean RCV (30.7 ± 4.3 to 32.7 ± 4.6 mL/kg) (all P < 0.05). Training tended to increase geometric mean iron absorption by 24% (P = 0.083), consistent with a decreased mean ± SD PHep (7.25 ± 2.14 to 5.17 ± 2.24 nM; P < 0.05). The increase in mHb and erythroid iron utilization were associated with the decrease in PHep (P < 0.05). Compartmental modeling indicated that iron for the increase in mHb was obtained predominantly (>80%) from stores mobilization rather than from increased dietary absorption. Conclusions In iron-sufficient men, mild intensification of exercise intensity increases both inflammation and erythropoiesis. The net effect is to decrease hepcidin concentrations and to tend to increase oral iron absorption. This trial was registered at clinicaltrials.gov as NCT01730521.

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