E. coli metabolic engineering for gram scale production of a plant-based anti-inflammatory agent

L-Fucose, one of the major monomeric sugars in brown algae, possesses high potential for use in the large-scale production of bio-based products. Although fucose catabolic pathways have been enzymatically evaluated, the effects of fucose as a carbon source on intracellular metabolism in industrial microorganisms such as Escherichia coli are still not identified. To elucidate the effects of fucose on cellular metabolism and to find clues for efficient conversion of fucose into bio-based products, comparative metabolomic and transcriptomic analyses were performed on E. coli on L-fucose and on D-glucose as a control. When fucose was the carbon source for E. coli, integration of the two omics analyses revealed that excess gluconeogenesis and quorum sensing led to severe depletion of ATP, resulting in accumulation and export of fucose extracellularly. Therefore, metabolic engineering and optimization are needed for E. coil to more efficiently ferment fucose. This is the first multi-omics study investigating the effects of fucose on cellular metabolism in E. coli. These omics data and their biological interpretation could be used to assist metabolic engineering of E. coli producing bio-based products using fucose-containing brown macroalgae.

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Use of a genetically engineered E. coli overexpressing β-glucuronidase accompanied by glycyrrhizic acid, a natural and anti-inflammatory agent, for directed treatment of colon carcinoma in a mouse model

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2020.119159 ◽

2020 ◽

Vol 579 ◽

pp. 119159 ◽

Cited By ~ 4

Author(s):

Amin Afkhami-Poostchi ◽

Mansour Mashreghi ◽

Mehrdad Iranshahi ◽

Maryam M. Matin

Keyword(s):

Mouse Model ◽

Colon Carcinoma ◽

Glycyrrhizic Acid ◽

Genetically Engineered ◽

E Coli ◽

Inflammatory Agent ◽

Anti Inflammatory

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Glucan particles as anti-inflammatory agent and drug delivery system for natural compounds in experimentally induced intestinal inflammation in vivo

10.1055/s-0037-1608375 ◽

2017 ◽

Author(s):

D Rotrekl ◽

Z Vochyánová ◽

L Paráková ◽

I Saloň ◽

P Šalamúnová ◽

...

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Delivery System ◽

Intestinal Inflammation ◽

Natural Compounds ◽

Inflammatory Agent ◽

Anti Inflammatory ◽

Experimentally Induced

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Faculty Opinions recommendation of Occupationally induced fixed drug eruption caused by a non-steroidal anti-inflammatory agent.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718023699.793479484 ◽

2013 ◽

Author(s):

Khalid Al Aboud

Keyword(s):

Drug Eruption ◽

Fixed Drug Eruption ◽

Inflammatory Agent ◽

Fixed Drug ◽

Anti Inflammatory

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Helenalin: An Anti-Inflammatory and Anti-Neoplastic Agent: A Review

Current Bioactive Compounds ◽

10.2174/1573407216666191226121004 ◽

2020 ◽

Vol 16 (8) ◽

pp. 1134-1146

Author(s):

Priyanka Kriplani ◽

Kumar Guarve ◽

Uttam S. Baghel

Keyword(s):

Rheumatoid Arthritis ◽

Adverse Effects ◽

Chronic Conditions ◽

Review Article ◽

Google Scholar ◽

Medical Community ◽

Inflammatory Agent ◽

Skin Cancers ◽

Medicinal Properties ◽

Anti Inflammatory

Objective: Helenalin is a natural anti-inflammatory agent that is proving its efficacy to treat various medical conditions. Though many plants are proving their effectiveness but their mechanisms are still not well understood. The objective of the review is to summarize various mechanisms of helenalin to treat inflammatory disorders and cancers, adverse effects, and avenues of further research. Methods: Structured research was carried out including Pub med, Science direct Medline, Research Gate and Google Scholar to find all articles published on helenalin. Various keywords used were “helenalin”, “Arnica”, “cancer”, “anti-inflammatory”, “cardiovascular”, “IBD”, “pharmacokinetics” etc. The aim of the review was to find out the problem prevailing in the data published to date which will help the researchers to investigate the molecule clinically. Results: Seventy articles are included in the review. Helenalin is found to cure chronic conditions like rheumatoid arthritis, ulcers and malignancies like stomach, colon, breast, larynx, lung and skin cancers via multiple mechanisms. These diseases do not proceed via a unilateral pathway. So, it can be a useful molecule to treat numerous diseases. Conclusion: This review article will help us to systemically analyze the wealth of information concerning the medicinal properties of helenalin and to recognize the gaps which have vetoed its pervasive application in the medical community.

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